P30620 (PSO2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA cross-link repair protein PSO2/SNM1
EC=3.1.-.-

Gene names

Name:	PSO2
Synonyms:	SNM1
Ordered Locus Names:	YMR137C
ORF Names:	YM9375.06C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	661 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for DNA interstrand cross-link repair. This requires cleavage of cross-linked DNA to generate DNA double strand breaks (DSBs). This protein has 5' exonuclease activity on single-stranded and double-stranded DNA, which appears to be necessary for the processing of DNA double strand breaks prior to ligation. Ref.6 Ref.7 Ref.9 Ref.11 Ref.12 Ref.13
Subcellular location	Nucleus Probable.
Induction	Expression is increased by ultraviolet light and agents which induce DNA cross-links such as nitrogen mustard and psoralen. Ref.5
Miscellaneous	Present with 259 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the DNA repair metallo-beta-lactamase (DRMBL) family.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Nucleus
Domain	Zinc-finger
Ligand	Magnesium Metal-binding Zinc
Molecular function	Exonuclease Hydrolase Nuclease
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	double-strand break repair Traceable author statement Ref.9. Source: SGD interstrand cross-link repair Inferred from mutant phenotype PubMed 22912599. Source: SGD
Cellular_component	nucleus Inferred from direct assay PubMed 22842922. Source: SGD
Molecular_function	5'-3' exonuclease activity Inferred from direct assay Ref.12. Source: SGD damaged DNA binding Inferred from mutant phenotype PubMed 7035927. Source: SGD metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 661	661	DNA cross-link repair protein PSO2/SNM1		PRO_0000209130
Regions
	Zinc finger	145 – 169	25	Potential
Amino acid modifications
	Modified residue	189	1	Phosphoserine Ref.14
	Modified residue	193	1	Phosphoserine Ref.14
Experimental info
	Mutagenesis	252	1	D → A: Abrogates exonuclease activity. Ref.9 Ref.12
	Mutagenesis	256	1	G → R in SNM1-2; increased sensitivity to DNA cross-linking agents at 36 degrees Celsius. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P30620 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 56F14DEBAC86EAE2	FASTA	661	76,399
	10 20 30 40 50 60 MSRKSIVQIR RSEVKRKRSS TASSTSEGKT LHKNTHTSSK RQRTLTEFNI PTSSNLPVRS 70 80 90 100 110 120 SSYSFSRFSC STSNKNTEPV IINDDDHNSI CLEDTAKVEI TIDTDEEELV SLHDNEVSAI 130 140 150 160 170 180 ENRTEDRIVT ELEEQVNVKV STEVIQCPIC LENLSHLELY ERETHCDTCI GSDPSNMGTP 190 200 210 220 230 240 KKNIRSFISN PSSPAKTKRD IATSKKPTRV KLVLPSFKII KFNNGHEIVV DGFNYKASET 250 260 270 280 290 300 ISQYFLSHFH SDHYIGLKKS WNNPDENPIK KTLYCSKITA ILVNLKFKIP MDEIQILPMN 310 320 330 340 350 360 KRFWITDTIS VVTLDANHCP GAIIMLFQEF LANSYDKPIR QILHTGDFRS NAKMIETIQK 370 380 390 400 410 420 WLAETANETI DQVYLDTTYM TMGYNFPSQH SVCETVADFT LRLIKHGKNK TFGDSQRNLF 430 440 450 460 470 480 HFQRKKTLTT HRYRVLFLVG TYTIGKEKLA IKICEFLKTK LFVMPNSVKF SMMLTVLQNN 490 500 510 520 530 540 ENQNDMWDES LLTSNLHESS VHLVPIRVLK SQETIEAYLK SLKELETDYV KDIEDVVGFI 550 560 570 580 590 600 PTGWSHNFGL KYQKKNDDDE NEMSGNTEYC LELMKNDRDN DDENGFEISS ILRQYKKYNK 610 620 630 640 650 660 FQVFNVPYSE HSSFNDLVKF GCKLKCSEVI PTVNLNNLWK VRYMTNWFQC WENVRKTRAA K

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular structure of the DNA cross-link repair gene SNM1 (PSO2) of the yeast Saccharomyces cerevisiae." Richter D., Niegemann E., Brendel M. Mol. Gen. Genet. 231:194-200(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII." Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. , Skelton J., Walsh S.V., Whitehead S., Barrell B.G. Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"A single amino acid change in SNM1-encoded protein leads to thermoconditional deficiency for DNA cross-link repair in Saccharomyces cerevisiae." Niegmann E., Brendel M. Mutat. Res. 315:275-279(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 252-467, MUTAGENESIS OF GLY-256.
[5]	"Regulation of SNM1, an inducible Saccharomyces cerevisiae gene required for repair of DNA cross-links." Wolter R., Siede W., Brendel M. Mol. Gen. Genet. 250:162-168(1996) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[6]	"Saccharomyces cerevisiae lacking Snm1, Rev3 or Rad51 have a normal S-phase but arrest permanently in G2 after cisplatin treatment." Grossmann K.F., Ward A.M., Moses R.E. Mutat. Res. 461:1-13(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[7]	"S. cerevisiae has three pathways for DNA interstrand crosslink repair." Grossmann K.F., Ward A.M., Matkovic M.E., Folias A.E., Moses R.E. Mutat. Res. 487:73-83(2001) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[8]	"Metallo-beta-lactamase fold within nucleic acids processing enzymes: the beta-CASP family." Callebaut I., Moshous D., Mornon J.-P., de Villartay J.-P. Nucleic Acids Res. 30:3592-3601(2002) [PubMed] [Europe PMC] [Abstract] Cited for: DNA REPAIR METALLO-BETA-LACTAMASE FAMILY.
[9]	"The beta-lactamase motif in Snm1 is required for repair of DNA double-strand breaks caused by interstrand crosslinks in S. cerevisiae." Li X., Moses R.E. DNA Repair 2:121-129(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF ASP-252.
[10]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]	"Microhomology-dependent end joining and repair of transposon-induced DNA hairpins by host factors in Saccharomyces cerevisiae." Yu J., Marshall K., Yamaguchi M., Haber J.E., Weil C.F. Mol. Cell. Biol. 24:1351-1364(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[12]	"The yeast Snm1 protein is a DNA 5'-exonuclease." Li X., Hejna J., Moses R.E. DNA Repair 4:163-170(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF ASP-252.
[13]	"DNA interstrand cross-link repair in the Saccharomyces cerevisiae cell cycle: overlapping roles for PSO2 (SNM1) with MutS factors and EXO1 during S phase." Barber L.J., Ward T.A., Hartley J.A., McHugh P.J. Mol. Cell. Biol. 25:2297-2309(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[14]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-189 AND SER-193, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X64004 Genomic DNA. Translation: CAA45405.1. Z47071 Genomic DNA. Translation: CAA87351.1. X76917 Genomic DNA. Translation: CAA54243.1. BK006946 Genomic DNA. Translation: DAA10034.1.
PIR	S19646.
RefSeq	NP_013857.1. NM_001182639.1.
3D structure databases
ProteinModelPortal	P30620.
SMR	P30620. Positions 211-652.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6301N.
IntAct	P30620. 2 interactions.
MINT	MINT-699963.
STRING	4932.YMR137C.
Proteomic databases
PaxDb	P30620.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YMR137C; YMR137C; YMR137C.
GeneID	855168.
KEGG	sce:YMR137C.
Organism-specific databases
CYGD	YMR137c.
SGD	S000004745. PSO2.
Phylogenomic databases
eggNOG	COG1236.
GeneTree	ENSGT00530000063183.
HOGENOM	HOG000115743.
KO	K15340.
OMA	SEHSSFK.
OrthoDB	EOG4M68SB.
Enzyme and pathway databases
BioCyc	YEAST:G3O-32830-MONOMER.
Gene expression databases
Genevestigator	P30620.
GermOnline	YMR137C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.60.15.10. 1 hit.
InterPro	IPR001279. Beta-lactamas-like. IPR011084. DRMBL. [Graphical view]
Pfam	PF07522. DRMBL. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	978602.

Entry information

Entry name

PSO2_YEAST

Accession

Primary (citable) accession number: P30620
Secondary accession number(s): D6VZW0, Q07072

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	May 29, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program