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P30620 (PSO2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA cross-link repair protein PSO2/SNM1

EC=3.1.-.-
Gene names
Name:PSO2
Synonyms:SNM1
Ordered Locus Names:YMR137C
ORF Names:YM9375.06C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length661 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for DNA interstrand cross-link repair. This requires cleavage of cross-linked DNA to generate DNA double strand breaks (DSBs). This protein has 5' exonuclease activity on single-stranded and double-stranded DNA, which appears to be necessary for the processing of DNA double strand breaks prior to ligation. Ref.6 Ref.7 Ref.9 Ref.11 Ref.12 Ref.13

Subcellular location

Nucleus Probable.

Induction

Expression is increased by ultraviolet light and agents which induce DNA cross-links such as nitrogen mustard and psoralen. Ref.5

Miscellaneous

Present with 259 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the DNA repair metallo-beta-lactamase (DRMBL) family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 661661DNA cross-link repair protein PSO2/SNM1
PRO_0000209130

Regions

Zinc finger145 – 16925 Potential

Experimental info

Mutagenesis2521D → A: Abrogates exonuclease activity. Ref.9 Ref.12
Mutagenesis2561G → R in SNM1-2; increased sensitivity to DNA cross-linking agents at 36 degrees Celsius. Ref.4

Sequences

Sequence LengthMass (Da)Tools
P30620 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: 56F14DEBAC86EAE2

FASTA66176,399
        10         20         30         40         50         60 
MSRKSIVQIR RSEVKRKRSS TASSTSEGKT LHKNTHTSSK RQRTLTEFNI PTSSNLPVRS 

        70         80         90        100        110        120 
SSYSFSRFSC STSNKNTEPV IINDDDHNSI CLEDTAKVEI TIDTDEEELV SLHDNEVSAI 

       130        140        150        160        170        180 
ENRTEDRIVT ELEEQVNVKV STEVIQCPIC LENLSHLELY ERETHCDTCI GSDPSNMGTP 

       190        200        210        220        230        240 
KKNIRSFISN PSSPAKTKRD IATSKKPTRV KLVLPSFKII KFNNGHEIVV DGFNYKASET 

       250        260        270        280        290        300 
ISQYFLSHFH SDHYIGLKKS WNNPDENPIK KTLYCSKITA ILVNLKFKIP MDEIQILPMN 

       310        320        330        340        350        360 
KRFWITDTIS VVTLDANHCP GAIIMLFQEF LANSYDKPIR QILHTGDFRS NAKMIETIQK 

       370        380        390        400        410        420 
WLAETANETI DQVYLDTTYM TMGYNFPSQH SVCETVADFT LRLIKHGKNK TFGDSQRNLF 

       430        440        450        460        470        480 
HFQRKKTLTT HRYRVLFLVG TYTIGKEKLA IKICEFLKTK LFVMPNSVKF SMMLTVLQNN 

       490        500        510        520        530        540 
ENQNDMWDES LLTSNLHESS VHLVPIRVLK SQETIEAYLK SLKELETDYV KDIEDVVGFI 

       550        560        570        580        590        600 
PTGWSHNFGL KYQKKNDDDE NEMSGNTEYC LELMKNDRDN DDENGFEISS ILRQYKKYNK 

       610        620        630        640        650        660 
FQVFNVPYSE HSSFNDLVKF GCKLKCSEVI PTVNLNNLWK VRYMTNWFQC WENVRKTRAA 


K 

« Hide

References

« Hide 'large scale' references
[1]"Molecular structure of the DNA cross-link repair gene SNM1 (PSO2) of the yeast Saccharomyces cerevisiae."
Richter D., Niegemann E., Brendel M.
Mol. Gen. Genet. 231:194-200(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII."
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P. expand/collapse author list , Skelton J., Walsh S.V., Whitehead S., Barrell B.G.
Nature 387:90-93(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"A single amino acid change in SNM1-encoded protein leads to thermoconditional deficiency for DNA cross-link repair in Saccharomyces cerevisiae."
Niegmann E., Brendel M.
Mutat. Res. 315:275-279(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 252-467, MUTAGENESIS OF GLY-256.
[5]"Regulation of SNM1, an inducible Saccharomyces cerevisiae gene required for repair of DNA cross-links."
Wolter R., Siede W., Brendel M.
Mol. Gen. Genet. 250:162-168(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[6]"Saccharomyces cerevisiae lacking Snm1, Rev3 or Rad51 have a normal S-phase but arrest permanently in G2 after cisplatin treatment."
Grossmann K.F., Ward A.M., Moses R.E.
Mutat. Res. 461:1-13(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"S. cerevisiae has three pathways for DNA interstrand crosslink repair."
Grossmann K.F., Ward A.M., Matkovic M.E., Folias A.E., Moses R.E.
Mutat. Res. 487:73-83(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[8]"Metallo-beta-lactamase fold within nucleic acids processing enzymes: the beta-CASP family."
Callebaut I., Moshous D., Mornon J.-P., de Villartay J.-P.
Nucleic Acids Res. 30:3592-3601(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: DNA REPAIR METALLO-BETA-LACTAMASE FAMILY.
[9]"The beta-lactamase motif in Snm1 is required for repair of DNA double-strand breaks caused by interstrand crosslinks in S. cerevisiae."
Li X., Moses R.E.
DNA Repair 2:121-129(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF ASP-252.
[10]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[11]"Microhomology-dependent end joining and repair of transposon-induced DNA hairpins by host factors in Saccharomyces cerevisiae."
Yu J., Marshall K., Yamaguchi M., Haber J.E., Weil C.F.
Mol. Cell. Biol. 24:1351-1364(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[12]"The yeast Snm1 protein is a DNA 5'-exonuclease."
Li X., Hejna J., Moses R.E.
DNA Repair 4:163-170(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF ASP-252.
[13]"DNA interstrand cross-link repair in the Saccharomyces cerevisiae cell cycle: overlapping roles for PSO2 (SNM1) with MutS factors and EXO1 during S phase."
Barber L.J., Ward T.A., Hartley J.A., McHugh P.J.
Mol. Cell. Biol. 25:2297-2309(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[14]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X64004 Genomic DNA. Translation: CAA45405.1.
Z47071 Genomic DNA. Translation: CAA87351.1.
X76917 Genomic DNA. Translation: CAA54243.1.
BK006946 Genomic DNA. Translation: DAA10034.1.
PIRS19646.
RefSeqNP_013857.1. NM_001182639.1.

3D structure databases

ProteinModelPortalP30620.
SMRP30620. Positions 211-652.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35314. 31 interactions.
DIPDIP-6301N.
MINTMINT-699963.
STRING4932.YMR137C.

Proteomic databases

PaxDbP30620.
PRIDEP30620.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYMR137C; YMR137C; YMR137C.
GeneID855168.
KEGGsce:YMR137C.

Organism-specific databases

CYGDYMR137c.
SGDS000004745. PSO2.

Phylogenomic databases

eggNOGCOG1236.
GeneTreeENSGT00530000063183.
HOGENOMHOG000115743.
KOK15340.
OMALYERETH.
OrthoDBEOG7VTDZ9.

Enzyme and pathway databases

BioCycYEAST:G3O-32830-MONOMER.

Gene expression databases

GenevestigatorP30620.

Family and domain databases

Gene3D3.60.15.10. 1 hit.
InterProIPR001279. Beta-lactamas-like.
IPR011084. DRMBL.
[Graphical view]
PfamPF07522. DRMBL. 1 hit.
[Graphical view]
SUPFAMSSF56281. SSF56281. 1 hit.
ProtoNetSearch...

Other

NextBio978602.

Entry information

Entry namePSO2_YEAST
AccessionPrimary (citable) accession number: P30620
Secondary accession number(s): D6VZW0, Q07072
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: April 16, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIII

Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families