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P30620

- PSO2_YEAST

UniProt

P30620 - PSO2_YEAST

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Protein

DNA cross-link repair protein PSO2/SNM1

Gene
PSO2, SNM1, YMR137C, YM9375.06C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Required for DNA interstrand cross-link repair. This requires cleavage of cross-linked DNA to generate DNA double strand breaks (DSBs). This protein has 5' exonuclease activity on single-stranded and double-stranded DNA, which appears to be necessary for the processing of DNA double strand breaks prior to ligation.6 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri145 – 16925 Reviewed predictionAdd
BLAST

GO - Molecular functioni

  1. 5'-3' exonuclease activity Source: SGD
  2. damaged DNA binding Source: SGD
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. DNA repair Source: SGD
  2. double-strand break repair Source: SGD
  3. interstrand cross-link repair Source: SGD
  4. nucleic acid phosphodiester bond hydrolysis Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Exonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-32830-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA cross-link repair protein PSO2/SNM1 (EC:3.1.-.-)
Gene namesi
Name:PSO2
Synonyms:SNM1
Ordered Locus Names:YMR137C
ORF Names:YM9375.06C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR137c.
SGDiS000004745. PSO2.

Subcellular locationi

Nucleus Inferred

GO - Cellular componenti

  1. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi252 – 2521D → A: Abrogates exonuclease activity. 2 Publications
Mutagenesisi256 – 2561G → R in SNM1-2; increased sensitivity to DNA cross-linking agents at 36 degrees Celsius. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 661661DNA cross-link repair protein PSO2/SNM1PRO_0000209130Add
BLAST

Proteomic databases

MaxQBiP30620.
PaxDbiP30620.
PRIDEiP30620.

Expressioni

Inductioni

Expression is increased by ultraviolet light and agents which induce DNA cross-links such as nitrogen mustard and psoralen.1 Publication

Gene expression databases

GenevestigatoriP30620.

Interactioni

Protein-protein interaction databases

BioGridi35314. 31 interactions.
DIPiDIP-6301N.
MINTiMINT-699963.
STRINGi4932.YMR137C.

Structurei

3D structure databases

ProteinModelPortaliP30620.
SMRiP30620. Positions 211-652.

Family & Domainsi

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri145 – 16925 Reviewed predictionAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG1236.
GeneTreeiENSGT00530000063183.
HOGENOMiHOG000115743.
KOiK15340.
OMAiLYERETH.
OrthoDBiEOG7VTDZ9.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
InterProiIPR001279. Beta-lactamas-like.
IPR011084. DRMBL.
[Graphical view]
PfamiPF07522. DRMBL. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.

Sequencei

Sequence statusi: Complete.

P30620-1 [UniParc]FASTAAdd to Basket

« Hide

MSRKSIVQIR RSEVKRKRSS TASSTSEGKT LHKNTHTSSK RQRTLTEFNI    50
PTSSNLPVRS SSYSFSRFSC STSNKNTEPV IINDDDHNSI CLEDTAKVEI 100
TIDTDEEELV SLHDNEVSAI ENRTEDRIVT ELEEQVNVKV STEVIQCPIC 150
LENLSHLELY ERETHCDTCI GSDPSNMGTP KKNIRSFISN PSSPAKTKRD 200
IATSKKPTRV KLVLPSFKII KFNNGHEIVV DGFNYKASET ISQYFLSHFH 250
SDHYIGLKKS WNNPDENPIK KTLYCSKITA ILVNLKFKIP MDEIQILPMN 300
KRFWITDTIS VVTLDANHCP GAIIMLFQEF LANSYDKPIR QILHTGDFRS 350
NAKMIETIQK WLAETANETI DQVYLDTTYM TMGYNFPSQH SVCETVADFT 400
LRLIKHGKNK TFGDSQRNLF HFQRKKTLTT HRYRVLFLVG TYTIGKEKLA 450
IKICEFLKTK LFVMPNSVKF SMMLTVLQNN ENQNDMWDES LLTSNLHESS 500
VHLVPIRVLK SQETIEAYLK SLKELETDYV KDIEDVVGFI PTGWSHNFGL 550
KYQKKNDDDE NEMSGNTEYC LELMKNDRDN DDENGFEISS ILRQYKKYNK 600
FQVFNVPYSE HSSFNDLVKF GCKLKCSEVI PTVNLNNLWK VRYMTNWFQC 650
WENVRKTRAA K 661
Length:661
Mass (Da):76,399
Last modified:April 1, 1993 - v1
Checksum:i56F14DEBAC86EAE2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X64004 Genomic DNA. Translation: CAA45405.1.
Z47071 Genomic DNA. Translation: CAA87351.1.
X76917 Genomic DNA. Translation: CAA54243.1.
BK006946 Genomic DNA. Translation: DAA10034.1.
PIRiS19646.
RefSeqiNP_013857.1. NM_001182639.1.

Genome annotation databases

EnsemblFungiiYMR137C; YMR137C; YMR137C.
GeneIDi855168.
KEGGisce:YMR137C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X64004 Genomic DNA. Translation: CAA45405.1 .
Z47071 Genomic DNA. Translation: CAA87351.1 .
X76917 Genomic DNA. Translation: CAA54243.1 .
BK006946 Genomic DNA. Translation: DAA10034.1 .
PIRi S19646.
RefSeqi NP_013857.1. NM_001182639.1.

3D structure databases

ProteinModelPortali P30620.
SMRi P30620. Positions 211-652.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35314. 31 interactions.
DIPi DIP-6301N.
MINTi MINT-699963.
STRINGi 4932.YMR137C.

Proteomic databases

MaxQBi P30620.
PaxDbi P30620.
PRIDEi P30620.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YMR137C ; YMR137C ; YMR137C .
GeneIDi 855168.
KEGGi sce:YMR137C.

Organism-specific databases

CYGDi YMR137c.
SGDi S000004745. PSO2.

Phylogenomic databases

eggNOGi COG1236.
GeneTreei ENSGT00530000063183.
HOGENOMi HOG000115743.
KOi K15340.
OMAi LYERETH.
OrthoDBi EOG7VTDZ9.

Enzyme and pathway databases

BioCyci YEAST:G3O-32830-MONOMER.

Miscellaneous databases

NextBioi 978602.

Gene expression databases

Genevestigatori P30620.

Family and domain databases

Gene3Di 3.60.15.10. 1 hit.
InterProi IPR001279. Beta-lactamas-like.
IPR011084. DRMBL.
[Graphical view ]
Pfami PF07522. DRMBL. 1 hit.
[Graphical view ]
SUPFAMi SSF56281. SSF56281. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular structure of the DNA cross-link repair gene SNM1 (PSO2) of the yeast Saccharomyces cerevisiae."
    Richter D., Niegemann E., Brendel M.
    Mol. Gen. Genet. 231:194-200(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "A single amino acid change in SNM1-encoded protein leads to thermoconditional deficiency for DNA cross-link repair in Saccharomyces cerevisiae."
    Niegmann E., Brendel M.
    Mutat. Res. 315:275-279(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 252-467, MUTAGENESIS OF GLY-256.
  5. "Regulation of SNM1, an inducible Saccharomyces cerevisiae gene required for repair of DNA cross-links."
    Wolter R., Siede W., Brendel M.
    Mol. Gen. Genet. 250:162-168(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  6. "Saccharomyces cerevisiae lacking Snm1, Rev3 or Rad51 have a normal S-phase but arrest permanently in G2 after cisplatin treatment."
    Grossmann K.F., Ward A.M., Moses R.E.
    Mutat. Res. 461:1-13(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "S. cerevisiae has three pathways for DNA interstrand crosslink repair."
    Grossmann K.F., Ward A.M., Matkovic M.E., Folias A.E., Moses R.E.
    Mutat. Res. 487:73-83(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Metallo-beta-lactamase fold within nucleic acids processing enzymes: the beta-CASP family."
    Callebaut I., Moshous D., Mornon J.-P., de Villartay J.-P.
    Nucleic Acids Res. 30:3592-3601(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: DNA REPAIR METALLO-BETA-LACTAMASE FAMILY.
  9. "The beta-lactamase motif in Snm1 is required for repair of DNA double-strand breaks caused by interstrand crosslinks in S. cerevisiae."
    Li X., Moses R.E.
    DNA Repair 2:121-129(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF ASP-252.
  10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  11. "Microhomology-dependent end joining and repair of transposon-induced DNA hairpins by host factors in Saccharomyces cerevisiae."
    Yu J., Marshall K., Yamaguchi M., Haber J.E., Weil C.F.
    Mol. Cell. Biol. 24:1351-1364(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. "The yeast Snm1 protein is a DNA 5'-exonuclease."
    Li X., Hejna J., Moses R.E.
    DNA Repair 4:163-170(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF ASP-252.
  13. "DNA interstrand cross-link repair in the Saccharomyces cerevisiae cell cycle: overlapping roles for PSO2 (SNM1) with MutS factors and EXO1 during S phase."
    Barber L.J., Ward T.A., Hartley J.A., McHugh P.J.
    Mol. Cell. Biol. 25:2297-2309(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  14. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPSO2_YEAST
AccessioniPrimary (citable) accession number: P30620
Secondary accession number(s): D6VZW0, Q07072
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: June 11, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 259 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

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