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Protein

DNA cross-link repair protein PSO2/SNM1

Gene

PSO2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for DNA interstrand cross-link repair. This requires cleavage of cross-linked DNA to generate DNA double strand breaks (DSBs). This protein has 5' exonuclease activity on single-stranded and double-stranded DNA, which appears to be necessary for the processing of DNA double strand breaks prior to ligation.6 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri145 – 169Sequence analysisAdd BLAST25

GO - Molecular functioni

  • 5'-3' exodeoxyribonuclease activity Source: GO_Central
  • 5'-3' exonuclease activity Source: SGD
  • damaged DNA binding Source: SGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • DNA repair Source: SGD
  • double-strand break repair Source: SGD
  • double-strand break repair via nonhomologous end joining Source: GO_Central
  • interstrand cross-link repair Source: SGD
  • protection from non-homologous end joining at telomere Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Exonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-32830-MONOMER.
ReactomeiR-SCE-5693571. Nonhomologous End-Joining (NHEJ).

Names & Taxonomyi

Protein namesi
Recommended name:
DNA cross-link repair protein PSO2/SNM1 (EC:3.1.-.-)
Gene namesi
Name:PSO2
Synonyms:SNM1
Ordered Locus Names:YMR137C
ORF Names:YM9375.06C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR137C.
SGDiS000004745. PSO2.

Subcellular locationi

GO - Cellular componenti

  • nuclear chromosome, telomeric region Source: GO_Central
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi252D → A: Abrogates exonuclease activity. 2 Publications1
Mutagenesisi256G → R in SNM1-2; increased sensitivity to DNA cross-linking agents at 36 degrees Celsius. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002091301 – 661DNA cross-link repair protein PSO2/SNM1Add BLAST661

Proteomic databases

MaxQBiP30620.
PRIDEiP30620.

PTM databases

iPTMnetiP30620.

Expressioni

Inductioni

Expression is increased by ultraviolet light and agents which induce DNA cross-links such as nitrogen mustard and psoralen.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi35314. 30 interactors.
DIPiDIP-6301N.
MINTiMINT-699963.

Structurei

3D structure databases

ProteinModelPortaliP30620.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri145 – 169Sequence analysisAdd BLAST25

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00530000063183.
HOGENOMiHOG000115743.
InParanoidiP30620.
KOiK15340.
OMAiLYERETH.
OrthoDBiEOG092C1ILX.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
InterProiIPR011084. DRMBL.
IPR001279. Metallo-B-lactamas.
[Graphical view]
PfamiPF07522. DRMBL. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.

Sequencei

Sequence statusi: Complete.

P30620-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRKSIVQIR RSEVKRKRSS TASSTSEGKT LHKNTHTSSK RQRTLTEFNI
60 70 80 90 100
PTSSNLPVRS SSYSFSRFSC STSNKNTEPV IINDDDHNSI CLEDTAKVEI
110 120 130 140 150
TIDTDEEELV SLHDNEVSAI ENRTEDRIVT ELEEQVNVKV STEVIQCPIC
160 170 180 190 200
LENLSHLELY ERETHCDTCI GSDPSNMGTP KKNIRSFISN PSSPAKTKRD
210 220 230 240 250
IATSKKPTRV KLVLPSFKII KFNNGHEIVV DGFNYKASET ISQYFLSHFH
260 270 280 290 300
SDHYIGLKKS WNNPDENPIK KTLYCSKITA ILVNLKFKIP MDEIQILPMN
310 320 330 340 350
KRFWITDTIS VVTLDANHCP GAIIMLFQEF LANSYDKPIR QILHTGDFRS
360 370 380 390 400
NAKMIETIQK WLAETANETI DQVYLDTTYM TMGYNFPSQH SVCETVADFT
410 420 430 440 450
LRLIKHGKNK TFGDSQRNLF HFQRKKTLTT HRYRVLFLVG TYTIGKEKLA
460 470 480 490 500
IKICEFLKTK LFVMPNSVKF SMMLTVLQNN ENQNDMWDES LLTSNLHESS
510 520 530 540 550
VHLVPIRVLK SQETIEAYLK SLKELETDYV KDIEDVVGFI PTGWSHNFGL
560 570 580 590 600
KYQKKNDDDE NEMSGNTEYC LELMKNDRDN DDENGFEISS ILRQYKKYNK
610 620 630 640 650
FQVFNVPYSE HSSFNDLVKF GCKLKCSEVI PTVNLNNLWK VRYMTNWFQC
660
WENVRKTRAA K
Length:661
Mass (Da):76,399
Last modified:April 1, 1993 - v1
Checksum:i56F14DEBAC86EAE2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64004 Genomic DNA. Translation: CAA45405.1.
Z47071 Genomic DNA. Translation: CAA87351.1.
X76917 Genomic DNA. Translation: CAA54243.1.
BK006946 Genomic DNA. Translation: DAA10034.1.
PIRiS19646.
RefSeqiNP_013857.1. NM_001182639.1.

Genome annotation databases

EnsemblFungiiYMR137C; YMR137C; YMR137C.
GeneIDi855168.
KEGGisce:YMR137C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64004 Genomic DNA. Translation: CAA45405.1.
Z47071 Genomic DNA. Translation: CAA87351.1.
X76917 Genomic DNA. Translation: CAA54243.1.
BK006946 Genomic DNA. Translation: DAA10034.1.
PIRiS19646.
RefSeqiNP_013857.1. NM_001182639.1.

3D structure databases

ProteinModelPortaliP30620.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35314. 30 interactors.
DIPiDIP-6301N.
MINTiMINT-699963.

PTM databases

iPTMnetiP30620.

Proteomic databases

MaxQBiP30620.
PRIDEiP30620.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR137C; YMR137C; YMR137C.
GeneIDi855168.
KEGGisce:YMR137C.

Organism-specific databases

EuPathDBiFungiDB:YMR137C.
SGDiS000004745. PSO2.

Phylogenomic databases

GeneTreeiENSGT00530000063183.
HOGENOMiHOG000115743.
InParanoidiP30620.
KOiK15340.
OMAiLYERETH.
OrthoDBiEOG092C1ILX.

Enzyme and pathway databases

BioCyciYEAST:G3O-32830-MONOMER.
ReactomeiR-SCE-5693571. Nonhomologous End-Joining (NHEJ).

Miscellaneous databases

PROiP30620.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
InterProiIPR011084. DRMBL.
IPR001279. Metallo-B-lactamas.
[Graphical view]
PfamiPF07522. DRMBL. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPSO2_YEAST
AccessioniPrimary (citable) accession number: P30620
Secondary accession number(s): D6VZW0, Q07072
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: November 2, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 259 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.