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P30620

- PSO2_YEAST

UniProt

P30620 - PSO2_YEAST

Protein

DNA cross-link repair protein PSO2/SNM1

Gene

PSO2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 Apr 1993)
      Previous versions | rss
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    Functioni

    Required for DNA interstrand cross-link repair. This requires cleavage of cross-linked DNA to generate DNA double strand breaks (DSBs). This protein has 5' exonuclease activity on single-stranded and double-stranded DNA, which appears to be necessary for the processing of DNA double strand breaks prior to ligation.6 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri145 – 16925Sequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. 5'-3' exonuclease activity Source: SGD
    2. damaged DNA binding Source: SGD
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. DNA repair Source: SGD
    2. double-strand break repair Source: SGD
    3. interstrand cross-link repair Source: SGD
    4. nucleic acid phosphodiester bond hydrolysis Source: GOC

    Keywords - Molecular functioni

    Exonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    DNA damage, DNA repair

    Keywords - Ligandi

    Magnesium, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32830-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA cross-link repair protein PSO2/SNM1 (EC:3.1.-.-)
    Gene namesi
    Name:PSO2
    Synonyms:SNM1
    Ordered Locus Names:YMR137C
    ORF Names:YM9375.06C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR137c.
    SGDiS000004745. PSO2.

    Subcellular locationi

    Nucleus Curated

    GO - Cellular componenti

    1. nucleus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi252 – 2521D → A: Abrogates exonuclease activity. 2 Publications
    Mutagenesisi256 – 2561G → R in SNM1-2; increased sensitivity to DNA cross-linking agents at 36 degrees Celsius. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 661661DNA cross-link repair protein PSO2/SNM1PRO_0000209130Add
    BLAST

    Proteomic databases

    MaxQBiP30620.
    PaxDbiP30620.
    PRIDEiP30620.

    Expressioni

    Inductioni

    Expression is increased by ultraviolet light and agents which induce DNA cross-links such as nitrogen mustard and psoralen.1 Publication

    Gene expression databases

    GenevestigatoriP30620.

    Interactioni

    Protein-protein interaction databases

    BioGridi35314. 31 interactions.
    DIPiDIP-6301N.
    MINTiMINT-699963.
    STRINGi4932.YMR137C.

    Structurei

    3D structure databases

    ProteinModelPortaliP30620.
    SMRiP30620. Positions 211-652.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri145 – 16925Sequence AnalysisAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG1236.
    GeneTreeiENSGT00530000063183.
    HOGENOMiHOG000115743.
    KOiK15340.
    OMAiLYERETH.
    OrthoDBiEOG7VTDZ9.

    Family and domain databases

    Gene3Di3.60.15.10. 1 hit.
    InterProiIPR001279. Beta-lactamas-like.
    IPR011084. DRMBL.
    [Graphical view]
    PfamiPF07522. DRMBL. 1 hit.
    [Graphical view]
    SUPFAMiSSF56281. SSF56281. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P30620-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSRKSIVQIR RSEVKRKRSS TASSTSEGKT LHKNTHTSSK RQRTLTEFNI    50
    PTSSNLPVRS SSYSFSRFSC STSNKNTEPV IINDDDHNSI CLEDTAKVEI 100
    TIDTDEEELV SLHDNEVSAI ENRTEDRIVT ELEEQVNVKV STEVIQCPIC 150
    LENLSHLELY ERETHCDTCI GSDPSNMGTP KKNIRSFISN PSSPAKTKRD 200
    IATSKKPTRV KLVLPSFKII KFNNGHEIVV DGFNYKASET ISQYFLSHFH 250
    SDHYIGLKKS WNNPDENPIK KTLYCSKITA ILVNLKFKIP MDEIQILPMN 300
    KRFWITDTIS VVTLDANHCP GAIIMLFQEF LANSYDKPIR QILHTGDFRS 350
    NAKMIETIQK WLAETANETI DQVYLDTTYM TMGYNFPSQH SVCETVADFT 400
    LRLIKHGKNK TFGDSQRNLF HFQRKKTLTT HRYRVLFLVG TYTIGKEKLA 450
    IKICEFLKTK LFVMPNSVKF SMMLTVLQNN ENQNDMWDES LLTSNLHESS 500
    VHLVPIRVLK SQETIEAYLK SLKELETDYV KDIEDVVGFI PTGWSHNFGL 550
    KYQKKNDDDE NEMSGNTEYC LELMKNDRDN DDENGFEISS ILRQYKKYNK 600
    FQVFNVPYSE HSSFNDLVKF GCKLKCSEVI PTVNLNNLWK VRYMTNWFQC 650
    WENVRKTRAA K 661
    Length:661
    Mass (Da):76,399
    Last modified:April 1, 1993 - v1
    Checksum:i56F14DEBAC86EAE2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X64004 Genomic DNA. Translation: CAA45405.1.
    Z47071 Genomic DNA. Translation: CAA87351.1.
    X76917 Genomic DNA. Translation: CAA54243.1.
    BK006946 Genomic DNA. Translation: DAA10034.1.
    PIRiS19646.
    RefSeqiNP_013857.1. NM_001182639.1.

    Genome annotation databases

    EnsemblFungiiYMR137C; YMR137C; YMR137C.
    GeneIDi855168.
    KEGGisce:YMR137C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X64004 Genomic DNA. Translation: CAA45405.1 .
    Z47071 Genomic DNA. Translation: CAA87351.1 .
    X76917 Genomic DNA. Translation: CAA54243.1 .
    BK006946 Genomic DNA. Translation: DAA10034.1 .
    PIRi S19646.
    RefSeqi NP_013857.1. NM_001182639.1.

    3D structure databases

    ProteinModelPortali P30620.
    SMRi P30620. Positions 211-652.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35314. 31 interactions.
    DIPi DIP-6301N.
    MINTi MINT-699963.
    STRINGi 4932.YMR137C.

    Proteomic databases

    MaxQBi P30620.
    PaxDbi P30620.
    PRIDEi P30620.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR137C ; YMR137C ; YMR137C .
    GeneIDi 855168.
    KEGGi sce:YMR137C.

    Organism-specific databases

    CYGDi YMR137c.
    SGDi S000004745. PSO2.

    Phylogenomic databases

    eggNOGi COG1236.
    GeneTreei ENSGT00530000063183.
    HOGENOMi HOG000115743.
    KOi K15340.
    OMAi LYERETH.
    OrthoDBi EOG7VTDZ9.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32830-MONOMER.

    Miscellaneous databases

    NextBioi 978602.

    Gene expression databases

    Genevestigatori P30620.

    Family and domain databases

    Gene3Di 3.60.15.10. 1 hit.
    InterProi IPR001279. Beta-lactamas-like.
    IPR011084. DRMBL.
    [Graphical view ]
    Pfami PF07522. DRMBL. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56281. SSF56281. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular structure of the DNA cross-link repair gene SNM1 (PSO2) of the yeast Saccharomyces cerevisiae."
      Richter D., Niegemann E., Brendel M.
      Mol. Gen. Genet. 231:194-200(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "A single amino acid change in SNM1-encoded protein leads to thermoconditional deficiency for DNA cross-link repair in Saccharomyces cerevisiae."
      Niegmann E., Brendel M.
      Mutat. Res. 315:275-279(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 252-467, MUTAGENESIS OF GLY-256.
    5. "Regulation of SNM1, an inducible Saccharomyces cerevisiae gene required for repair of DNA cross-links."
      Wolter R., Siede W., Brendel M.
      Mol. Gen. Genet. 250:162-168(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.
    6. "Saccharomyces cerevisiae lacking Snm1, Rev3 or Rad51 have a normal S-phase but arrest permanently in G2 after cisplatin treatment."
      Grossmann K.F., Ward A.M., Moses R.E.
      Mutat. Res. 461:1-13(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "S. cerevisiae has three pathways for DNA interstrand crosslink repair."
      Grossmann K.F., Ward A.M., Matkovic M.E., Folias A.E., Moses R.E.
      Mutat. Res. 487:73-83(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "Metallo-beta-lactamase fold within nucleic acids processing enzymes: the beta-CASP family."
      Callebaut I., Moshous D., Mornon J.-P., de Villartay J.-P.
      Nucleic Acids Res. 30:3592-3601(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: DNA REPAIR METALLO-BETA-LACTAMASE FAMILY.
    9. "The beta-lactamase motif in Snm1 is required for repair of DNA double-strand breaks caused by interstrand crosslinks in S. cerevisiae."
      Li X., Moses R.E.
      DNA Repair 2:121-129(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF ASP-252.
    10. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    11. "Microhomology-dependent end joining and repair of transposon-induced DNA hairpins by host factors in Saccharomyces cerevisiae."
      Yu J., Marshall K., Yamaguchi M., Haber J.E., Weil C.F.
      Mol. Cell. Biol. 24:1351-1364(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    12. "The yeast Snm1 protein is a DNA 5'-exonuclease."
      Li X., Hejna J., Moses R.E.
      DNA Repair 4:163-170(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF ASP-252.
    13. "DNA interstrand cross-link repair in the Saccharomyces cerevisiae cell cycle: overlapping roles for PSO2 (SNM1) with MutS factors and EXO1 during S phase."
      Barber L.J., Ward T.A., Hartley J.A., McHugh P.J.
      Mol. Cell. Biol. 25:2297-2309(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    14. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPSO2_YEAST
    AccessioniPrimary (citable) accession number: P30620
    Secondary accession number(s): D6VZW0, Q07072
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: April 1, 1993
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 259 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3