Reviewed, UniProtKB/Swiss-Prot P30616 (KPYK2_TRYBB)

Names and origin

Protein names

Recommended name:
    Pyruvate kinase 2
      Short name=PK 2
    EC=2.7.1.40

Gene names

Name:

PYK2

Organism

Trypanosoma brucei brucei

Taxonomic identifier

5702 [NCBI]

Taxonomic lineage

Eukaryota › Euglenozoa › Kinetoplastida › Trypanosomatidae › Trypanosoma

Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + pyruvate = ADP + phosphoenolpyruvate.
Cofactor	Magnesium. Potassium.
Enzyme regulation	Activated by fructose 2,6-bisphosphate, activated by the effector in a cooperative manner.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the pyruvate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Pyruvate
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW potassium ion binding Inferred from electronic annotation. Source: InterPro pyruvate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 499	499	Pyruvate kinase 2		PRO_0000112104
Sites
	Active site	239	1	By similarity
	Metal binding	241	1	Magnesium Potential
	Metal binding	262	1	Magnesium Potential
	Metal binding	263	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	P30616-1 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 7FE8238E73E45CA2	FASTA	499	54,452
	10 20 30 40 50 60 MSQLEHNIGL SIFEPVAKHR ANRIVCTIGP STQSVEALKN LMKSGMSVAR MNFSHGSHEY 70 80 90 100 110 120 HQTTINNVRA AAAELGLHIG IALDTKGPEI RTGLFKDGEV TFAPGDIVCV TTDPAYEKVG 130 140 150 160 170 180 TKEKFYIDYP QLTKAVPVGG SIYVDDGVMT LRVLSKEDDR TLKCHVNNHH RLTDRRGINL 190 200 210 220 230 240 PGCEVDLPAV SEKDRKDLEF GVAQGVDMIF ASFIRTAEQV REVRAALGEK GKDILIISKI 250 260 270 280 290 300 ENHQGVQNID SIIEASNGIM VARGDLGVEI PAEKVCVAQM CIISKCNVVG KPVICATQML 310 320 330 340 350 360 ESMTSNPRPT RAEVSDVANA VLNGADCVML SGETAKGKYP NEVVQYMARI CVEAQSATHD 370 380 390 400 410 420 TVMFNSIKNL QKIPMCPEEA VCSSAVASAF EVQAKAMLVL SNTGRSARLI SKYRPNCPII 430 440 450 460 470 480 CVTTRLQTCR QLNVTRSVVS VFYDAAKSGE DKDKEKRVKL GLDFAKKEKY ASTGDVVVVV 490 HADHSVKGYP NQTRLIYLP

References

[1]	"Molecular cloning and analysis of two tandemly linked genes for pyruvate kinase of Trypanosoma brucei." Allert S., Ernest I., Poliszczak A., Opperdoes F.R., Michels P.A.M. Eur. J. Biochem. 200:19-27(1991) [PubMed: 1879424] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 427.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X57951 Genomic DNA. Translation: CAA41019.1.
PIR	S17649.
3D structure databases
HSSP	HSSP built from PDB template 1PKL based on UniProtKB Q27686.
SMR	P30616. Positions 2-496.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.1.40. 74165.
Family and domain databases
InterPro	IPR001697. Pyr_Knase. IPR015813. Pyrv/PenolPyrv_Kinase_cat. IPR015794. Pyrv_Knase_a/b. IPR018209. Pyrv_Knase_AS. IPR015793. Pyrv_Knase_brl. [Graphical view]
Gene3D	G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit. G3DSA:3.40.1380.20. Pyrv_Knase_a/b. 1 hit.
PANTHER	PTHR11817. Pyruvate_kinase. 1 hit.
Pfam	PF00224. PK. 1 hit. PF02887. PK_C. 1 hit. [Graphical view]
PRINTS	PR01050. PYRUVTKNASE.
ProDom	PD001009. Pyruvate_kinase. 2 hits. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01064. pyruv_kin. 1 hit.
PROSITE	PS00110. PYRUVATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPYK2_TRYBB

Accession

Primary (citable) accession number: P30616

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	June 16, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)