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P30616

- KPYK2_TRYBB

UniProt

P30616 - KPYK2_TRYBB

Protein

Pyruvate kinase 2

Gene

PYK2

Organism
Trypanosoma brucei brucei
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (01 Apr 1993)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + pyruvate = ADP + phosphoenolpyruvate.

    Cofactori

    Magnesium.
    Potassium.

    Enzyme regulationi

    Activated by fructose 2,6-bisphosphate, activated by the effector in a cooperative manner.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei50 – 501SubstrateBy similarity
    Metal bindingi52 – 521PotassiumBy similarity
    Metal bindingi54 – 541PotassiumBy similarity
    Metal bindingi84 – 841PotassiumBy similarity
    Metal bindingi85 – 851Potassium; via carbonyl oxygenBy similarity
    Sitei239 – 2391Transition state stabilizerBy similarity
    Metal bindingi241 – 2411MagnesiumSequence Analysis
    Binding sitei264 – 2641Substrate; via amide nitrogenBy similarity
    Metal bindingi265 – 2651MagnesiumBy similarity
    Binding sitei265 – 2651Substrate; via amide nitrogenBy similarity
    Binding sitei297 – 2971SubstrateBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. magnesium ion binding Source: InterPro
    3. potassium ion binding Source: InterPro
    4. pyruvate kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

    Enzyme and pathway databases

    SABIO-RKP30616.
    UniPathwayiUPA00109; UER00188.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate kinase 2 (EC:2.7.1.40)
    Short name:
    PK 2
    Gene namesi
    Name:PYK2
    OrganismiTrypanosoma brucei brucei
    Taxonomic identifieri5702 [NCBI]
    Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosoma

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 499499Pyruvate kinase 2PRO_0000112104Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP30616.
    SMRiP30616. Positions 2-496.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the pyruvate kinase family.Curated

    Family and domain databases

    Gene3Di2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProiIPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view]
    PANTHERiPTHR11817. PTHR11817. 1 hit.
    PfamiPF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view]
    PRINTSiPR01050. PYRUVTKNASE.
    SUPFAMiSSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
    PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P30616-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQLEHNIGL SIFEPVAKHR ANRIVCTIGP STQSVEALKN LMKSGMSVAR    50
    MNFSHGSHEY HQTTINNVRA AAAELGLHIG IALDTKGPEI RTGLFKDGEV 100
    TFAPGDIVCV TTDPAYEKVG TKEKFYIDYP QLTKAVPVGG SIYVDDGVMT 150
    LRVLSKEDDR TLKCHVNNHH RLTDRRGINL PGCEVDLPAV SEKDRKDLEF 200
    GVAQGVDMIF ASFIRTAEQV REVRAALGEK GKDILIISKI ENHQGVQNID 250
    SIIEASNGIM VARGDLGVEI PAEKVCVAQM CIISKCNVVG KPVICATQML 300
    ESMTSNPRPT RAEVSDVANA VLNGADCVML SGETAKGKYP NEVVQYMARI 350
    CVEAQSATHD TVMFNSIKNL QKIPMCPEEA VCSSAVASAF EVQAKAMLVL 400
    SNTGRSARLI SKYRPNCPII CVTTRLQTCR QLNVTRSVVS VFYDAAKSGE 450
    DKDKEKRVKL GLDFAKKEKY ASTGDVVVVV HADHSVKGYP NQTRLIYLP 499
    Length:499
    Mass (Da):54,452
    Last modified:April 1, 1993 - v1
    Checksum:i7FE8238E73E45CA2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X57951 Genomic DNA. Translation: CAA41019.1.
    PIRiS17649.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X57951 Genomic DNA. Translation: CAA41019.1 .
    PIRi S17649.

    3D structure databases

    ProteinModelPortali P30616.
    SMRi P30616. Positions 2-496.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00188 .
    SABIO-RK P30616.

    Family and domain databases

    Gene3Di 2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProi IPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view ]
    PANTHERi PTHR11817. PTHR11817. 1 hit.
    Pfami PF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view ]
    PRINTSi PR01050. PYRUVTKNASE.
    SUPFAMi SSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
    PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and analysis of two tandemly linked genes for pyruvate kinase of Trypanosoma brucei."
      Allert S., Ernest I., Poliszczak A., Opperdoes F.R., Michels P.A.M.
      Eur. J. Biochem. 200:19-27(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 427.

    Entry informationi

    Entry nameiKPYK2_TRYBB
    AccessioniPrimary (citable) accession number: P30616
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: April 1, 1993
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3