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P30616 (KPYK2_TRYBB) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pyruvate kinase 2

Short name=PK 2
EC=2.7.1.40
Gene names
Name:PYK2
OrganismTrypanosoma brucei brucei
Taxonomic identifier5702 [NCBI]
Taxonomic lineageEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosoma

Protein attributes

Sequence length499 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactor

Magnesium.

Potassium.

Enzyme regulation

Activated by fructose 2,6-bisphosphate, activated by the effector in a cooperative manner.

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.

Subunit structure

Homotetramer By similarity.

Sequence similarities

Belongs to the pyruvate kinase family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 499499Pyruvate kinase 2
PRO_0000112104

Sites

Metal binding521Potassium By similarity
Metal binding541Potassium By similarity
Metal binding841Potassium By similarity
Metal binding851Potassium; via carbonyl oxygen By similarity
Metal binding2411Magnesium Potential
Metal binding2651Magnesium By similarity
Binding site501Substrate By similarity
Binding site2641Substrate; via amide nitrogen By similarity
Binding site2651Substrate; via amide nitrogen By similarity
Binding site2971Substrate By similarity
Site2391Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
P30616 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: 7FE8238E73E45CA2

FASTA49954,452
        10         20         30         40         50         60 
MSQLEHNIGL SIFEPVAKHR ANRIVCTIGP STQSVEALKN LMKSGMSVAR MNFSHGSHEY 

        70         80         90        100        110        120 
HQTTINNVRA AAAELGLHIG IALDTKGPEI RTGLFKDGEV TFAPGDIVCV TTDPAYEKVG 

       130        140        150        160        170        180 
TKEKFYIDYP QLTKAVPVGG SIYVDDGVMT LRVLSKEDDR TLKCHVNNHH RLTDRRGINL 

       190        200        210        220        230        240 
PGCEVDLPAV SEKDRKDLEF GVAQGVDMIF ASFIRTAEQV REVRAALGEK GKDILIISKI 

       250        260        270        280        290        300 
ENHQGVQNID SIIEASNGIM VARGDLGVEI PAEKVCVAQM CIISKCNVVG KPVICATQML 

       310        320        330        340        350        360 
ESMTSNPRPT RAEVSDVANA VLNGADCVML SGETAKGKYP NEVVQYMARI CVEAQSATHD 

       370        380        390        400        410        420 
TVMFNSIKNL QKIPMCPEEA VCSSAVASAF EVQAKAMLVL SNTGRSARLI SKYRPNCPII 

       430        440        450        460        470        480 
CVTTRLQTCR QLNVTRSVVS VFYDAAKSGE DKDKEKRVKL GLDFAKKEKY ASTGDVVVVV 

       490 
HADHSVKGYP NQTRLIYLP 

« Hide

References

[1]"Molecular cloning and analysis of two tandemly linked genes for pyruvate kinase of Trypanosoma brucei."
Allert S., Ernest I., Poliszczak A., Opperdoes F.R., Michels P.A.M.
Eur. J. Biochem. 200:19-27(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 427.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X57951 Genomic DNA. Translation: CAA41019.1.
PIRS17649.

3D structure databases

ProteinModelPortalP30616.
SMRP30616. Positions 2-496.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

SABIO-RKP30616.
UniPathwayUPA00109; UER00188.

Family and domain databases

Gene3D2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERPTHR11817. PTHR11817. 1 hit.
PfamPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSPR01050. PYRUVTKNASE.
SUPFAMSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsTIGR01064. pyruv_kin. 1 hit.
PROSITEPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKPYK2_TRYBB
AccessionPrimary (citable) accession number: P30616
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: June 11, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways