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P30615

- KPYK1_TRYBB

UniProt

P30615 - KPYK1_TRYBB

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Protein

Pyruvate kinase 1

Gene

PYK1

Organism
Trypanosoma brucei brucei
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:

Enzyme regulationi

Activated by fructose 2,6-bisphosphate, activated by the effector in a cooperative manner.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei50 – 501SubstrateBy similarity
Metal bindingi52 – 521PotassiumBy similarity
Metal bindingi54 – 541PotassiumBy similarity
Metal bindingi84 – 841PotassiumBy similarity
Metal bindingi85 – 851Potassium; via carbonyl oxygenBy similarity
Sitei239 – 2391Transition state stabilizerBy similarity
Metal bindingi241 – 2411MagnesiumSequence Analysis
Binding sitei264 – 2641Substrate; via amide nitrogenBy similarity
Metal bindingi265 – 2651MagnesiumBy similarity
Binding sitei265 – 2651Substrate; via amide nitrogenBy similarity
Binding sitei297 – 2971SubstrateBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: InterPro
  3. potassium ion binding Source: InterPro
  4. pyruvate kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

SABIO-RKP30615.
UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase 1 (EC:2.7.1.40)
Short name:
PK 1
Gene namesi
Name:PYK1
OrganismiTrypanosoma brucei brucei
Taxonomic identifieri5702 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosoma

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 499499Pyruvate kinase 1PRO_0000112103Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

Secondary structure

1
499
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 86Combined sources
Beta strandi12 – 143Combined sources
Beta strandi22 – 276Combined sources
Turni30 – 323Combined sources
Helixi35 – 4410Combined sources
Beta strandi46 – 527Combined sources
Helixi58 – 7518Combined sources
Beta strandi80 – 845Combined sources
Beta strandi90 – 923Combined sources
Helixi96 – 983Combined sources
Beta strandi99 – 1024Combined sources
Beta strandi107 – 1115Combined sources
Helixi114 – 1163Combined sources
Beta strandi122 – 1287Combined sources
Helixi132 – 1354Combined sources
Beta strandi141 – 1444Combined sources
Turni145 – 1484Combined sources
Beta strandi149 – 15810Combined sources
Beta strandi161 – 1666Combined sources
Beta strandi170 – 1734Combined sources
Beta strandi177 – 1793Combined sources
Helixi192 – 20413Combined sources
Beta strandi207 – 2115Combined sources
Helixi217 – 22711Combined sources
Turni228 – 2336Combined sources
Beta strandi234 – 2407Combined sources
Helixi243 – 2475Combined sources
Helixi249 – 2557Combined sources
Beta strandi256 – 2627Combined sources
Helixi263 – 2697Combined sources
Helixi272 – 2743Combined sources
Helixi275 – 28915Combined sources
Beta strandi293 – 2986Combined sources
Helixi301 – 3044Combined sources
Beta strandi306 – 3083Combined sources
Helixi311 – 32313Combined sources
Beta strandi326 – 3316Combined sources
Helixi332 – 3354Combined sources
Helixi340 – 35617Combined sources
Helixi362 – 3698Combined sources
Helixi377 – 39216Combined sources
Beta strandi395 – 4006Combined sources
Beta strandi402 – 4043Combined sources
Helixi405 – 4128Combined sources
Beta strandi419 – 4246Combined sources
Helixi426 – 4316Combined sources
Helixi432 – 4343Combined sources
Beta strandi438 – 4425Combined sources
Helixi445 – 4484Combined sources
Helixi455 – 46713Combined sources
Beta strandi476 – 4816Combined sources
Beta strandi492 – 4976Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4HYVX-ray2.30A/B1-499[»]
4HYWX-ray2.35A/B1-499[»]
4KCTX-ray1.95A/B1-499[»]
4KCUX-ray2.35A/B1-499[»]
4KCVX-ray2.18A/B1-499[»]
4KCWX-ray2.50A/B1-499[»]
ProteinModelPortaliP30615.
SMRiP30615. Positions 2-496.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P30615-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSQLEHNIGL SIFEPVAKHR ANRIVCTIGP STQSVEALKN LMKSGMSVAR
60 70 80 90 100
MNFSHGSHEY HQTTINNVRA AAAELGLHIG IALDTKGPEI RTGLFKDGEV
110 120 130 140 150
SFAPGDIVCV TTDPAYEKVG TKEKFYIDYP QLTNAVRPGG SIYVDDGVMT
160 170 180 190 200
LRVVSKEDDR TLKCHVNNHH RLTDRRGINL PGCEVDLPAV SEKDRKDLEF
210 220 230 240 250
GVAQGVDMIF ASFIRTAEQV REVRAALGEK GKDILIISKI ENHQGVQNID
260 270 280 290 300
SIIEASNGIM VARGDLGVEI PAEKVCVAQM CIISKCNVVG KPVICATQML
310 320 330 340 350
ESMTSNPRPT RAEVSDVANA VLNGADCVML SGETAKGKYP NEVVQYMARI
360 370 380 390 400
CVEAQSATHD TVMFNSIKNL QKIPMCPEEA VCSSAVASAF EVQAKAMLVL
410 420 430 440 450
SNTGRSARLI SKYRPNCPII CVTTRLQTCR QLNVTRSVVS VFYDAAKSGE
460 470 480 490
DKDKEKRVKL GLDFAKKEKY ASTGDVVVVV HADHSVKGYP NQTRLIYLP
Length:499
Mass (Da):54,467
Last modified:April 1, 1993 - v1
Checksum:i1D40AD5352B272C6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57950 Genomic DNA. Translation: CAA41018.1.
PIRiS17648.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57950 Genomic DNA. Translation: CAA41018.1 .
PIRi S17648.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4HYV X-ray 2.30 A/B 1-499 [» ]
4HYW X-ray 2.35 A/B 1-499 [» ]
4KCT X-ray 1.95 A/B 1-499 [» ]
4KCU X-ray 2.35 A/B 1-499 [» ]
4KCV X-ray 2.18 A/B 1-499 [» ]
4KCW X-ray 2.50 A/B 1-499 [» ]
ProteinModelPortali P30615.
SMRi P30615. Positions 2-496.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00188 .
SABIO-RK P30615.

Family and domain databases

Gene3Di 2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProi IPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view ]
PANTHERi PTHR11817. PTHR11817. 1 hit.
Pfami PF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view ]
PRINTSi PR01050. PYRUVTKNASE.
SUPFAMi SSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and analysis of two tandemly linked genes for pyruvate kinase of Trypanosoma brucei."
    Allert S., Ernest I., Poliszczak A., Opperdoes F.R., Michels P.A.M.
    Eur. J. Biochem. 200:19-27(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 427.

Entry informationi

Entry nameiKPYK1_TRYBB
AccessioniPrimary (citable) accession number: P30615
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: November 26, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Allosteric enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3