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P30615

- KPYK1_TRYBB

UniProt

P30615 - KPYK1_TRYBB

Protein

Pyruvate kinase 1

Gene

PYK1

Organism
Trypanosoma brucei brucei
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (01 Apr 1993)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + pyruvate = ADP + phosphoenolpyruvate.

    Cofactori

    Magnesium.
    Potassium.

    Enzyme regulationi

    Activated by fructose 2,6-bisphosphate, activated by the effector in a cooperative manner.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei50 – 501SubstrateBy similarity
    Metal bindingi52 – 521PotassiumBy similarity
    Metal bindingi54 – 541PotassiumBy similarity
    Metal bindingi84 – 841PotassiumBy similarity
    Metal bindingi85 – 851Potassium; via carbonyl oxygenBy similarity
    Sitei239 – 2391Transition state stabilizerBy similarity
    Metal bindingi241 – 2411MagnesiumSequence Analysis
    Binding sitei264 – 2641Substrate; via amide nitrogenBy similarity
    Metal bindingi265 – 2651MagnesiumBy similarity
    Binding sitei265 – 2651Substrate; via amide nitrogenBy similarity
    Binding sitei297 – 2971SubstrateBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. magnesium ion binding Source: InterPro
    3. potassium ion binding Source: InterPro
    4. pyruvate kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

    Enzyme and pathway databases

    SABIO-RKP30615.
    UniPathwayiUPA00109; UER00188.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate kinase 1 (EC:2.7.1.40)
    Short name:
    PK 1
    Gene namesi
    Name:PYK1
    OrganismiTrypanosoma brucei brucei
    Taxonomic identifieri5702 [NCBI]
    Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosoma

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 499499Pyruvate kinase 1PRO_0000112103Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Structurei

    Secondary structure

    1
    499
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi3 – 86
    Beta strandi12 – 143
    Beta strandi22 – 276
    Turni30 – 323
    Helixi35 – 4410
    Beta strandi46 – 527
    Helixi58 – 7518
    Beta strandi80 – 845
    Beta strandi90 – 923
    Helixi96 – 983
    Beta strandi99 – 1024
    Beta strandi107 – 1115
    Helixi114 – 1163
    Beta strandi122 – 1287
    Helixi132 – 1354
    Beta strandi141 – 1444
    Turni145 – 1484
    Beta strandi149 – 15810
    Beta strandi161 – 1666
    Beta strandi170 – 1734
    Beta strandi177 – 1793
    Helixi192 – 20413
    Beta strandi207 – 2115
    Helixi217 – 22711
    Turni228 – 2336
    Beta strandi234 – 2407
    Helixi243 – 2475
    Helixi249 – 2557
    Beta strandi256 – 2627
    Helixi263 – 2697
    Helixi272 – 2743
    Helixi275 – 28915
    Beta strandi293 – 2986
    Helixi301 – 3044
    Beta strandi306 – 3083
    Helixi311 – 32313
    Beta strandi326 – 3316
    Helixi332 – 3354
    Helixi340 – 35617
    Helixi362 – 3698
    Helixi377 – 39216
    Beta strandi395 – 4006
    Beta strandi402 – 4043
    Helixi405 – 4128
    Beta strandi419 – 4246
    Helixi426 – 4316
    Helixi432 – 4343
    Beta strandi438 – 4425
    Helixi445 – 4484
    Helixi455 – 46713
    Beta strandi476 – 4816
    Beta strandi492 – 4976

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4HYVX-ray2.30A/B1-499[»]
    4HYWX-ray2.35A/B1-499[»]
    4KCTX-ray1.95A/B1-499[»]
    4KCUX-ray2.35A/B1-499[»]
    4KCVX-ray2.18A/B1-499[»]
    4KCWX-ray2.50A/B1-499[»]
    ProteinModelPortaliP30615.
    SMRiP30615. Positions 2-496.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the pyruvate kinase family.Curated

    Family and domain databases

    Gene3Di2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProiIPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view]
    PANTHERiPTHR11817. PTHR11817. 1 hit.
    PfamiPF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view]
    PRINTSiPR01050. PYRUVTKNASE.
    SUPFAMiSSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
    PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P30615-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSQLEHNIGL SIFEPVAKHR ANRIVCTIGP STQSVEALKN LMKSGMSVAR    50
    MNFSHGSHEY HQTTINNVRA AAAELGLHIG IALDTKGPEI RTGLFKDGEV 100
    SFAPGDIVCV TTDPAYEKVG TKEKFYIDYP QLTNAVRPGG SIYVDDGVMT 150
    LRVVSKEDDR TLKCHVNNHH RLTDRRGINL PGCEVDLPAV SEKDRKDLEF 200
    GVAQGVDMIF ASFIRTAEQV REVRAALGEK GKDILIISKI ENHQGVQNID 250
    SIIEASNGIM VARGDLGVEI PAEKVCVAQM CIISKCNVVG KPVICATQML 300
    ESMTSNPRPT RAEVSDVANA VLNGADCVML SGETAKGKYP NEVVQYMARI 350
    CVEAQSATHD TVMFNSIKNL QKIPMCPEEA VCSSAVASAF EVQAKAMLVL 400
    SNTGRSARLI SKYRPNCPII CVTTRLQTCR QLNVTRSVVS VFYDAAKSGE 450
    DKDKEKRVKL GLDFAKKEKY ASTGDVVVVV HADHSVKGYP NQTRLIYLP 499
    Length:499
    Mass (Da):54,467
    Last modified:April 1, 1993 - v1
    Checksum:i1D40AD5352B272C6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X57950 Genomic DNA. Translation: CAA41018.1.
    PIRiS17648.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X57950 Genomic DNA. Translation: CAA41018.1 .
    PIRi S17648.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4HYV X-ray 2.30 A/B 1-499 [» ]
    4HYW X-ray 2.35 A/B 1-499 [» ]
    4KCT X-ray 1.95 A/B 1-499 [» ]
    4KCU X-ray 2.35 A/B 1-499 [» ]
    4KCV X-ray 2.18 A/B 1-499 [» ]
    4KCW X-ray 2.50 A/B 1-499 [» ]
    ProteinModelPortali P30615.
    SMRi P30615. Positions 2-496.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00188 .
    SABIO-RK P30615.

    Family and domain databases

    Gene3Di 2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProi IPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view ]
    PANTHERi PTHR11817. PTHR11817. 1 hit.
    Pfami PF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view ]
    PRINTSi PR01050. PYRUVTKNASE.
    SUPFAMi SSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
    PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and analysis of two tandemly linked genes for pyruvate kinase of Trypanosoma brucei."
      Allert S., Ernest I., Poliszczak A., Opperdoes F.R., Michels P.A.M.
      Eur. J. Biochem. 200:19-27(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 427.

    Entry informationi

    Entry nameiKPYK1_TRYBB
    AccessioniPrimary (citable) accession number: P30615
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: April 1, 1993
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Allosteric enzyme

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3