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P30615

- KPYK1_TRYBB

UniProt

P30615 - KPYK1_TRYBB

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Protein

Pyruvate kinase 1

Gene
PYK1
Organism
Trypanosoma brucei brucei
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Magnesium.
Potassium.

Enzyme regulationi

Activated by fructose 2,6-bisphosphate, activated by the effector in a cooperative manner.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei50 – 501Substrate By similarity
Metal bindingi52 – 521Potassium By similarity
Metal bindingi54 – 541Potassium By similarity
Metal bindingi84 – 841Potassium By similarity
Metal bindingi85 – 851Potassium; via carbonyl oxygen By similarity
Sitei239 – 2391Transition state stabilizer By similarity
Metal bindingi241 – 2411Magnesium Reviewed prediction
Binding sitei264 – 2641Substrate; via amide nitrogen By similarity
Metal bindingi265 – 2651Magnesium By similarity
Binding sitei265 – 2651Substrate; via amide nitrogen By similarity
Binding sitei297 – 2971Substrate By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: InterPro
  3. potassium ion binding Source: InterPro
  4. pyruvate kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

SABIO-RKP30615.
UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase 1 (EC:2.7.1.40)
Short name:
PK 1
Gene namesi
Name:PYK1
OrganismiTrypanosoma brucei brucei
Taxonomic identifieri5702 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeTrypanosoma

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 499499Pyruvate kinase 1PRO_0000112103Add
BLAST

Interactioni

Subunit structurei

Homotetramer By similarity.

Structurei

Secondary structure

1
499
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 86
Beta strandi12 – 143
Beta strandi22 – 276
Turni30 – 323
Helixi35 – 4410
Beta strandi46 – 527
Helixi58 – 7518
Beta strandi80 – 845
Beta strandi90 – 923
Helixi96 – 983
Beta strandi99 – 1024
Beta strandi107 – 1115
Helixi114 – 1163
Beta strandi122 – 1287
Helixi132 – 1354
Beta strandi141 – 1444
Turni145 – 1484
Beta strandi149 – 15810
Beta strandi161 – 1666
Beta strandi170 – 1734
Beta strandi177 – 1793
Helixi192 – 20413
Beta strandi207 – 2115
Helixi217 – 22711
Turni228 – 2336
Beta strandi234 – 2407
Helixi243 – 2475
Helixi249 – 2557
Beta strandi256 – 2627
Helixi263 – 2697
Helixi272 – 2743
Helixi275 – 28915
Beta strandi293 – 2986
Helixi301 – 3044
Beta strandi306 – 3083
Helixi311 – 32313
Beta strandi326 – 3316
Helixi332 – 3354
Helixi340 – 35617
Helixi362 – 3698
Helixi377 – 39216
Beta strandi395 – 4006
Beta strandi402 – 4043
Helixi405 – 4128
Beta strandi419 – 4246
Helixi426 – 4316
Helixi432 – 4343
Beta strandi438 – 4425
Helixi445 – 4484
Helixi455 – 46713
Beta strandi476 – 4816
Beta strandi492 – 4976

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4HYVX-ray2.30A/B1-499[»]
4HYWX-ray2.35A/B1-499[»]
4KCTX-ray1.95A/B1-499[»]
4KCUX-ray2.35A/B1-499[»]
4KCVX-ray2.18A/B1-499[»]
4KCWX-ray2.50A/B1-499[»]
ProteinModelPortaliP30615.
SMRiP30615. Positions 2-496.

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P30615-1 [UniParc]FASTAAdd to Basket

« Hide

MSQLEHNIGL SIFEPVAKHR ANRIVCTIGP STQSVEALKN LMKSGMSVAR    50
MNFSHGSHEY HQTTINNVRA AAAELGLHIG IALDTKGPEI RTGLFKDGEV 100
SFAPGDIVCV TTDPAYEKVG TKEKFYIDYP QLTNAVRPGG SIYVDDGVMT 150
LRVVSKEDDR TLKCHVNNHH RLTDRRGINL PGCEVDLPAV SEKDRKDLEF 200
GVAQGVDMIF ASFIRTAEQV REVRAALGEK GKDILIISKI ENHQGVQNID 250
SIIEASNGIM VARGDLGVEI PAEKVCVAQM CIISKCNVVG KPVICATQML 300
ESMTSNPRPT RAEVSDVANA VLNGADCVML SGETAKGKYP NEVVQYMARI 350
CVEAQSATHD TVMFNSIKNL QKIPMCPEEA VCSSAVASAF EVQAKAMLVL 400
SNTGRSARLI SKYRPNCPII CVTTRLQTCR QLNVTRSVVS VFYDAAKSGE 450
DKDKEKRVKL GLDFAKKEKY ASTGDVVVVV HADHSVKGYP NQTRLIYLP 499
Length:499
Mass (Da):54,467
Last modified:April 1, 1993 - v1
Checksum:i1D40AD5352B272C6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X57950 Genomic DNA. Translation: CAA41018.1.
PIRiS17648.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X57950 Genomic DNA. Translation: CAA41018.1 .
PIRi S17648.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4HYV X-ray 2.30 A/B 1-499 [» ]
4HYW X-ray 2.35 A/B 1-499 [» ]
4KCT X-ray 1.95 A/B 1-499 [» ]
4KCU X-ray 2.35 A/B 1-499 [» ]
4KCV X-ray 2.18 A/B 1-499 [» ]
4KCW X-ray 2.50 A/B 1-499 [» ]
ProteinModelPortali P30615.
SMRi P30615. Positions 2-496.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00188 .
SABIO-RK P30615.

Family and domain databases

Gene3Di 2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProi IPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view ]
PANTHERi PTHR11817. PTHR11817. 1 hit.
Pfami PF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view ]
PRINTSi PR01050. PYRUVTKNASE.
SUPFAMi SSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and analysis of two tandemly linked genes for pyruvate kinase of Trypanosoma brucei."
    Allert S., Ernest I., Poliszczak A., Opperdoes F.R., Michels P.A.M.
    Eur. J. Biochem. 200:19-27(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 427.

Entry informationi

Entry nameiKPYK1_TRYBB
AccessioniPrimary (citable) accession number: P30615
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: June 11, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Allosteric enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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