Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P30614

- KPYK_YARLI

UniProt

P30614 - KPYK_YARLI

Protein

Pyruvate kinase

Gene

PYK1

Organism
Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 3 (11 Oct 2004)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    ATP + pyruvate = ADP + phosphoenolpyruvate.

    Cofactori

    Magnesium.
    Potassium.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei60 – 601SubstrateBy similarity
    Metal bindingi62 – 621PotassiumBy similarity
    Metal bindingi64 – 641PotassiumBy similarity
    Metal bindingi95 – 951PotassiumBy similarity
    Metal bindingi96 – 961Potassium; via carbonyl oxygenBy similarity
    Sitei251 – 2511Transition state stabilizerBy similarity
    Metal bindingi253 – 2531MagnesiumBy similarity
    Binding sitei276 – 2761Substrate; via amide nitrogenBy similarity
    Metal bindingi277 – 2771MagnesiumBy similarity
    Binding sitei277 – 2771Substrate; via amide nitrogenBy similarity
    Binding sitei309 – 3091SubstrateBy similarity
    Binding sitei348 – 3481ADPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. magnesium ion binding Source: InterPro
    3. potassium ion binding Source: InterPro
    4. pyruvate kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

    Enzyme and pathway databases

    UniPathwayiUPA00109; UER00188.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate kinase (EC:2.7.1.40)
    Short name:
    PK
    Gene namesi
    Name:PYK1
    Ordered Locus Names:YALI0F09185g
    OrganismiYarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
    Taxonomic identifieri284591 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDipodascaceaeYarrowia
    ProteomesiUP000001300: Chromosome F

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 515515Pyruvate kinasePRO_0000112120Add
    BLAST

    Proteomic databases

    PRIDEiP30614.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    STRINGi4952.P30614.

    Structurei

    3D structure databases

    ProteinModelPortaliP30614.
    SMRiP30614. Positions 28-511.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the pyruvate kinase family.Curated

    Phylogenomic databases

    eggNOGiCOG0469.
    HOGENOMiHOG000021559.
    KOiK00873.
    OrthoDBiEOG7M6DJC.

    Family and domain databases

    Gene3Di2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProiIPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view]
    PANTHERiPTHR11817. PTHR11817. 1 hit.
    PfamiPF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view]
    PRINTSiPR01050. PYRUVTKNASE.
    SUPFAMiSSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
    PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P30614-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIYTANSSPS TNLQWLSTLN TDDIPTKNYR KSSIIGTIGP NTNSAEMISK    50
    LRQAGLNIVR MNFSHGSYEY HQSVIENARE SEQQFQGRPL AIALDTKGPE 100
    IRTGVTKDDK DWDVKAGHVM LFSTNPKYKD QCDDKIMYID YTNIVKQIDI 150
    GKIIFVDDGV LSFKVLEKID GETLKVETLN NGKISSRKGV NLPGTDVDLP 200
    ALSEKDKADL KFGVEHGVDM IFASFVRTAN DVQAIRDVLG EKGKGIQVIS 250
    KIENQQGVNN FDEILKETDG VMVARGDLGI EIPAPQVFIA QKQLIAKCNL 300
    AGKPVICATQ MLDSMTYNPR PTRAEVSDVG NAVLDGADCV MLSGETAKGT 350
    YPIESVKMMH ETCLVAEKAI AYAPLFNEMR TLTVRPTETV ETIAISAVSA 400
    SFEQQARAII VLSTSGTSAR LCSKYRPNCP ILMVTRNAQA ARFSHLYRGV 450
    YPFIYHKARA SNPAEWQHDV EERLKWGMDE AVALGILNKG DVVVAIQGWT 500
    GGLGHTNTLR VLECV 515
    Length:515
    Mass (Da):56,668
    Last modified:October 11, 2004 - v3
    Checksum:iED3E51220306F1D6
    GO

    Sequence cautioni

    The sequence AAA18520.1 differs from that shown. Reason: Frameshift at position 504.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti84 – 863QFQ → RFR in AAA18520. (PubMed:1511886)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M86863 Unassigned DNA. Translation: AAA18520.1. Frameshift.
    CR382132 Genomic DNA. Translation: CAG78002.2.
    PIRiJC1267.
    RefSeqiXP_505195.2. XM_505195.2.

    Genome annotation databases

    EnsemblFungiiCAG78002; CAG78002; YALI0_F09185g.
    GeneIDi2907873.
    KEGGiyli:YALI0F09185g.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M86863 Unassigned DNA. Translation: AAA18520.1 . Frameshift.
    CR382132 Genomic DNA. Translation: CAG78002.2 .
    PIRi JC1267.
    RefSeqi XP_505195.2. XM_505195.2.

    3D structure databases

    ProteinModelPortali P30614.
    SMRi P30614. Positions 28-511.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 4952.P30614.

    Proteomic databases

    PRIDEi P30614.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii CAG78002 ; CAG78002 ; YALI0_F09185g .
    GeneIDi 2907873.
    KEGGi yli:YALI0F09185g.

    Phylogenomic databases

    eggNOGi COG0469.
    HOGENOMi HOG000021559.
    KOi K00873.
    OrthoDBi EOG7M6DJC.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00188 .

    Family and domain databases

    Gene3Di 2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProi IPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view ]
    PANTHERi PTHR11817. PTHR11817. 1 hit.
    Pfami PF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view ]
    PRINTSi PR01050. PYRUVTKNASE.
    SUPFAMi SSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
    PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The isolation and characterization of the pyruvate kinase-encoding gene from the yeast Yarrowia lipolytica."
      Strick C.A., James L.C., O'Donnell M.M., Gollaher M.G., Franke A.E.
      Gene 118:65-72(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: ATCC 8662 / IFO 1209 / NRRL Y-1094.
    2. "Genome evolution in yeasts."
      Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.
      , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L.
      Nature 430:35-44(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CLIB 122 / E 150.

    Entry informationi

    Entry nameiKPYK_YARLI
    AccessioniPrimary (citable) accession number: P30614
    Secondary accession number(s): Q6C2B7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: October 11, 2004
    Last modified: October 1, 2014
    This is version 104 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3