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P30614

- KPYK_YARLI

UniProt

P30614 - KPYK_YARLI

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Protein

Pyruvate kinase

Gene

PYK1

Organism
Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Magnesium.
Potassium.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei60 – 601SubstrateBy similarity
Metal bindingi62 – 621PotassiumBy similarity
Metal bindingi64 – 641PotassiumBy similarity
Metal bindingi95 – 951PotassiumBy similarity
Metal bindingi96 – 961Potassium; via carbonyl oxygenBy similarity
Sitei251 – 2511Transition state stabilizerBy similarity
Metal bindingi253 – 2531MagnesiumBy similarity
Binding sitei276 – 2761Substrate; via amide nitrogenBy similarity
Metal bindingi277 – 2771MagnesiumBy similarity
Binding sitei277 – 2771Substrate; via amide nitrogenBy similarity
Binding sitei309 – 3091SubstrateBy similarity
Binding sitei348 – 3481ADPSequence Analysis

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: InterPro
  3. potassium ion binding Source: InterPro
  4. pyruvate kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase (EC:2.7.1.40)
Short name:
PK
Gene namesi
Name:PYK1
Ordered Locus Names:YALI0F09185g
OrganismiYarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
Taxonomic identifieri284591 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDipodascaceaeYarrowia
ProteomesiUP000001300: Chromosome F

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 515515Pyruvate kinasePRO_0000112120Add
BLAST

Proteomic databases

PRIDEiP30614.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi4952.P30614.

Structurei

3D structure databases

ProteinModelPortaliP30614.
SMRiP30614. Positions 28-511.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

eggNOGiCOG0469.
HOGENOMiHOG000021559.
InParanoidiP30614.
KOiK00873.
OrthoDBiEOG7M6DJC.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P30614-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIYTANSSPS TNLQWLSTLN TDDIPTKNYR KSSIIGTIGP NTNSAEMISK
60 70 80 90 100
LRQAGLNIVR MNFSHGSYEY HQSVIENARE SEQQFQGRPL AIALDTKGPE
110 120 130 140 150
IRTGVTKDDK DWDVKAGHVM LFSTNPKYKD QCDDKIMYID YTNIVKQIDI
160 170 180 190 200
GKIIFVDDGV LSFKVLEKID GETLKVETLN NGKISSRKGV NLPGTDVDLP
210 220 230 240 250
ALSEKDKADL KFGVEHGVDM IFASFVRTAN DVQAIRDVLG EKGKGIQVIS
260 270 280 290 300
KIENQQGVNN FDEILKETDG VMVARGDLGI EIPAPQVFIA QKQLIAKCNL
310 320 330 340 350
AGKPVICATQ MLDSMTYNPR PTRAEVSDVG NAVLDGADCV MLSGETAKGT
360 370 380 390 400
YPIESVKMMH ETCLVAEKAI AYAPLFNEMR TLTVRPTETV ETIAISAVSA
410 420 430 440 450
SFEQQARAII VLSTSGTSAR LCSKYRPNCP ILMVTRNAQA ARFSHLYRGV
460 470 480 490 500
YPFIYHKARA SNPAEWQHDV EERLKWGMDE AVALGILNKG DVVVAIQGWT
510
GGLGHTNTLR VLECV
Length:515
Mass (Da):56,668
Last modified:October 11, 2004 - v3
Checksum:iED3E51220306F1D6
GO

Sequence cautioni

The sequence AAA18520.1 differs from that shown. Reason: Frameshift at position 504.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti84 – 863QFQ → RFR in AAA18520. (PubMed:1511886)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M86863 Unassigned DNA. Translation: AAA18520.1. Frameshift.
CR382132 Genomic DNA. Translation: CAG78002.2.
PIRiJC1267.
RefSeqiXP_505195.2. XM_505195.2.

Genome annotation databases

EnsemblFungiiCAG78002; CAG78002; YALI0_F09185g.
GeneIDi2907873.
KEGGiyli:YALI0F09185g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M86863 Unassigned DNA. Translation: AAA18520.1 . Frameshift.
CR382132 Genomic DNA. Translation: CAG78002.2 .
PIRi JC1267.
RefSeqi XP_505195.2. XM_505195.2.

3D structure databases

ProteinModelPortali P30614.
SMRi P30614. Positions 28-511.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 4952.P30614.

Proteomic databases

PRIDEi P30614.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii CAG78002 ; CAG78002 ; YALI0_F09185g .
GeneIDi 2907873.
KEGGi yli:YALI0F09185g.

Phylogenomic databases

eggNOGi COG0469.
HOGENOMi HOG000021559.
InParanoidi P30614.
KOi K00873.
OrthoDBi EOG7M6DJC.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00188 .

Family and domain databases

Gene3Di 2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProi IPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view ]
PANTHERi PTHR11817. PTHR11817. 1 hit.
Pfami PF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view ]
PRINTSi PR01050. PYRUVTKNASE.
SUPFAMi SSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The isolation and characterization of the pyruvate kinase-encoding gene from the yeast Yarrowia lipolytica."
    Strick C.A., James L.C., O'Donnell M.M., Gollaher M.G., Franke A.E.
    Gene 118:65-72(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: ATCC 8662 / IFO 1209 / NRRL Y-1094.
  2. "Genome evolution in yeasts."
    Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.
    , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L.
    Nature 430:35-44(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CLIB 122 / E 150.

Entry informationi

Entry nameiKPYK_YARLI
AccessioniPrimary (citable) accession number: P30614
Secondary accession number(s): Q6C2B7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: October 11, 2004
Last modified: October 29, 2014
This is version 105 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3