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Protein

Pyruvate kinase

Gene

PYK1

Organism
Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:

Pathway:iglycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (GPD)
  2. Phosphoglycerate kinase (PGK1)
  3. no protein annotated in this organism
  4. Enolase (ENO)
  5. Pyruvate kinase (PYK1)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei60 – 601SubstrateBy similarity
Metal bindingi62 – 621PotassiumBy similarity
Metal bindingi64 – 641PotassiumBy similarity
Metal bindingi95 – 951PotassiumBy similarity
Metal bindingi96 – 961Potassium; via carbonyl oxygenBy similarity
Sitei251 – 2511Transition state stabilizerBy similarity
Metal bindingi253 – 2531MagnesiumBy similarity
Binding sitei276 – 2761Substrate; via amide nitrogenBy similarity
Metal bindingi277 – 2771MagnesiumBy similarity
Binding sitei277 – 2771Substrate; via amide nitrogenBy similarity
Binding sitei309 – 3091SubstrateBy similarity
Binding sitei348 – 3481ADPSequence Analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

BRENDAi2.7.1.40. 1122.
UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase (EC:2.7.1.40)
Short name:
PK
Gene namesi
Name:PYK1
Ordered Locus Names:YALI0F09185g
OrganismiYarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
Taxonomic identifieri284591 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDipodascaceaeYarrowia
ProteomesiUP000001300 Componenti: Chromosome F

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 515515Pyruvate kinasePRO_0000112120Add
BLAST

Proteomic databases

PRIDEiP30614.

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP30614.
SMRiP30614. Positions 28-511.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

eggNOGiCOG0469.
HOGENOMiHOG000021559.
InParanoidiP30614.
KOiK00873.
OMAiCRIAYET.
OrthoDBiEOG7M6DJC.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P30614-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIYTANSSPS TNLQWLSTLN TDDIPTKNYR KSSIIGTIGP NTNSAEMISK
60 70 80 90 100
LRQAGLNIVR MNFSHGSYEY HQSVIENARE SEQQFQGRPL AIALDTKGPE
110 120 130 140 150
IRTGVTKDDK DWDVKAGHVM LFSTNPKYKD QCDDKIMYID YTNIVKQIDI
160 170 180 190 200
GKIIFVDDGV LSFKVLEKID GETLKVETLN NGKISSRKGV NLPGTDVDLP
210 220 230 240 250
ALSEKDKADL KFGVEHGVDM IFASFVRTAN DVQAIRDVLG EKGKGIQVIS
260 270 280 290 300
KIENQQGVNN FDEILKETDG VMVARGDLGI EIPAPQVFIA QKQLIAKCNL
310 320 330 340 350
AGKPVICATQ MLDSMTYNPR PTRAEVSDVG NAVLDGADCV MLSGETAKGT
360 370 380 390 400
YPIESVKMMH ETCLVAEKAI AYAPLFNEMR TLTVRPTETV ETIAISAVSA
410 420 430 440 450
SFEQQARAII VLSTSGTSAR LCSKYRPNCP ILMVTRNAQA ARFSHLYRGV
460 470 480 490 500
YPFIYHKARA SNPAEWQHDV EERLKWGMDE AVALGILNKG DVVVAIQGWT
510
GGLGHTNTLR VLECV
Length:515
Mass (Da):56,668
Last modified:October 11, 2004 - v3
Checksum:iED3E51220306F1D6
GO

Sequence cautioni

The sequence AAA18520.1 differs from that shown. Reason: Frameshift at position 504. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti84 – 863QFQ → RFR in AAA18520 (PubMed:1511886).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86863 Unassigned DNA. Translation: AAA18520.1. Frameshift.
CR382132 Genomic DNA. Translation: CAG78002.2.
PIRiJC1267.
RefSeqiXP_505195.2. XM_505195.2.

Genome annotation databases

EnsemblFungiiCAG78002; CAG78002; YALI0_F09185g.
GeneIDi2907873.
KEGGiyli:YALI0F09185g.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86863 Unassigned DNA. Translation: AAA18520.1. Frameshift.
CR382132 Genomic DNA. Translation: CAG78002.2.
PIRiJC1267.
RefSeqiXP_505195.2. XM_505195.2.

3D structure databases

ProteinModelPortaliP30614.
SMRiP30614. Positions 28-511.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP30614.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCAG78002; CAG78002; YALI0_F09185g.
GeneIDi2907873.
KEGGiyli:YALI0F09185g.

Phylogenomic databases

eggNOGiCOG0469.
HOGENOMiHOG000021559.
InParanoidiP30614.
KOiK00873.
OMAiCRIAYET.
OrthoDBiEOG7M6DJC.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.
BRENDAi2.7.1.40. 1122.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The isolation and characterization of the pyruvate kinase-encoding gene from the yeast Yarrowia lipolytica."
    Strick C.A., James L.C., O'Donnell M.M., Gollaher M.G., Franke A.E.
    Gene 118:65-72(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: ATCC 8662 / IFO 1209 / NRRL Y-1094.
  2. "Genome evolution in yeasts."
    Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.
    , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L.
    Nature 430:35-44(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CLIB 122 / E 150.

Entry informationi

Entry nameiKPYK_YARLI
AccessioniPrimary (citable) accession number: P30614
Secondary accession number(s): Q6C2B7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: October 11, 2004
Last modified: July 22, 2015
This is version 110 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.