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P30614 (KPYK_YARLI) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pyruvate kinase

Short name=PK
EC=2.7.1.40
Gene names
Name:PYK1
Ordered Locus Names:YALI0F09185g
OrganismYarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica) [Reference proteome]
Taxonomic identifier284591 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDipodascaceaeYarrowia

Protein attributes

Sequence length515 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactor

Magnesium.

Potassium.

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.

Subunit structure

Homotetramer By similarity.

Sequence similarities

Belongs to the pyruvate kinase family.

Sequence caution

The sequence AAA18520.1 differs from that shown. Reason: Frameshift at position 504.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 515515Pyruvate kinase
PRO_0000112120

Sites

Metal binding621Potassium By similarity
Metal binding641Potassium By similarity
Metal binding951Potassium By similarity
Metal binding961Potassium; via carbonyl oxygen By similarity
Metal binding2531Magnesium By similarity
Metal binding2771Magnesium By similarity
Binding site601Substrate By similarity
Binding site2761Substrate; via amide nitrogen By similarity
Binding site2771Substrate; via amide nitrogen By similarity
Binding site3091Substrate By similarity
Binding site3481ADP Potential
Site2511Transition state stabilizer By similarity

Experimental info

Sequence conflict84 – 863QFQ → RFR in AAA18520. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P30614 [UniParc].

Last modified October 11, 2004. Version 3.
Checksum: ED3E51220306F1D6

FASTA51556,668
        10         20         30         40         50         60 
MIYTANSSPS TNLQWLSTLN TDDIPTKNYR KSSIIGTIGP NTNSAEMISK LRQAGLNIVR 

        70         80         90        100        110        120 
MNFSHGSYEY HQSVIENARE SEQQFQGRPL AIALDTKGPE IRTGVTKDDK DWDVKAGHVM 

       130        140        150        160        170        180 
LFSTNPKYKD QCDDKIMYID YTNIVKQIDI GKIIFVDDGV LSFKVLEKID GETLKVETLN 

       190        200        210        220        230        240 
NGKISSRKGV NLPGTDVDLP ALSEKDKADL KFGVEHGVDM IFASFVRTAN DVQAIRDVLG 

       250        260        270        280        290        300 
EKGKGIQVIS KIENQQGVNN FDEILKETDG VMVARGDLGI EIPAPQVFIA QKQLIAKCNL 

       310        320        330        340        350        360 
AGKPVICATQ MLDSMTYNPR PTRAEVSDVG NAVLDGADCV MLSGETAKGT YPIESVKMMH 

       370        380        390        400        410        420 
ETCLVAEKAI AYAPLFNEMR TLTVRPTETV ETIAISAVSA SFEQQARAII VLSTSGTSAR 

       430        440        450        460        470        480 
LCSKYRPNCP ILMVTRNAQA ARFSHLYRGV YPFIYHKARA SNPAEWQHDV EERLKWGMDE 

       490        500        510 
AVALGILNKG DVVVAIQGWT GGLGHTNTLR VLECV 

« Hide

References

« Hide 'large scale' references
[1]"The isolation and characterization of the pyruvate kinase-encoding gene from the yeast Yarrowia lipolytica."
Strick C.A., James L.C., O'Donnell M.M., Gollaher M.G., Franke A.E.
Gene 118:65-72(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: ATCC 8662 / IFO 1209 / NRRL Y-1094.
[2]Erratum
Strick C.A., James L.C., O'Donnell M.M., Gollaher M.G., Franke A.E.
Gene 140:141-143(1994) [PubMed] [Europe PMC] [Abstract]
[3]"Genome evolution in yeasts."
Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. expand/collapse author list , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L.
Nature 430:35-44(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CLIB 122 / E 150.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M86863 Unassigned DNA. Translation: AAA18520.1. Frameshift.
CR382132 Genomic DNA. Translation: CAG78002.2.
PIRJC1267.
RefSeqXP_505195.2. XM_505195.2.

3D structure databases

ProteinModelPortalP30614.
SMRP30614. Positions 28-511.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING4952.P30614.

Proteomic databases

PRIDEP30614.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiCAG78002; CAG78002; YALI0_F09185g.
GeneID2907873.
KEGGyli:YALI0F09185g.

Phylogenomic databases

eggNOGCOG0469.
HOGENOMHOG000021559.
KOK00873.
OrthoDBEOG7M6DJC.

Enzyme and pathway databases

UniPathwayUPA00109; UER00188.

Family and domain databases

Gene3D2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERPTHR11817. PTHR11817. 1 hit.
PfamPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSPR01050. PYRUVTKNASE.
SUPFAMSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsTIGR01064. pyruv_kin. 1 hit.
PROSITEPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKPYK_YARLI
AccessionPrimary (citable) accession number: P30614
Secondary accession number(s): Q6C2B7
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: October 11, 2004
Last modified: November 13, 2013
This is version 102 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways