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Protein

Pyruvate kinase

Gene

PYK1

Organism
Yarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:

Pathwayi: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (GPD)
  2. Phosphoglycerate kinase (PGK1)
  3. Phosphoglycerate mutase (YALI0_B02728g)
  4. Enolase (ENO)
  5. Pyruvate kinase (PYK1)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei60SubstrateBy similarity1
Metal bindingi62PotassiumBy similarity1
Metal bindingi64PotassiumBy similarity1
Metal bindingi95PotassiumBy similarity1
Metal bindingi96Potassium; via carbonyl oxygenBy similarity1
Sitei251Transition state stabilizerBy similarity1
Metal bindingi253MagnesiumBy similarity1
Binding sitei276Substrate; via amide nitrogenBy similarity1
Metal bindingi277MagnesiumBy similarity1
Binding sitei277Substrate; via amide nitrogenBy similarity1
Binding sitei309SubstrateBy similarity1
Binding sitei348ADPSequence analysis1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

BRENDAi2.7.1.40 1122
UniPathwayiUPA00109; UER00188

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase (EC:2.7.1.40)
Short name:
PK
Gene namesi
Name:PYK1
Ordered Locus Names:YALI0F09185g
OrganismiYarrowia lipolytica (strain CLIB 122 / E 150) (Yeast) (Candida lipolytica)
Taxonomic identifieri284591 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDipodascaceaeYarrowia
Proteomesi
  • UP000001300 Componenti: Chromosome F

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001121201 – 515Pyruvate kinaseAdd BLAST515

Proteomic databases

PRIDEiP30614

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi4952.XP_505195.2

Structurei

3D structure databases

ProteinModelPortaliP30614
SMRiP30614
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

HOGENOMiHOG000021559
InParanoidiP30614
KOiK00873
OMAiGSHDDIR
OrthoDBiEOG092C1RXZ

Family and domain databases

CDDicd00288 Pyruvate_Kinase, 1 hit
Gene3Di2.40.33.10, 1 hit
3.40.1380.20, 2 hits
InterProiView protein in InterPro
IPR001697 Pyr_Knase
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
IPR011037 Pyrv_Knase-like_insert_dom_sf
IPR018209 Pyrv_Knase_AS
IPR015793 Pyrv_Knase_brl
IPR015795 Pyrv_Knase_C
IPR036918 Pyrv_Knase_C_sf
IPR015806 Pyrv_Knase_insert_dom_sf
PANTHERiPTHR11817 PTHR11817, 1 hit
PfamiView protein in Pfam
PF00224 PK, 1 hit
PF02887 PK_C, 1 hit
PRINTSiPR01050 PYRUVTKNASE
SUPFAMiSSF50800 SSF50800, 1 hit
SSF51621 SSF51621, 2 hits
SSF52935 SSF52935, 1 hit
TIGRFAMsiTIGR01064 pyruv_kin, 1 hit
PROSITEiView protein in PROSITE
PS00110 PYRUVATE_KINASE, 1 hit

Sequencei

Sequence statusi: Complete.

P30614-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIYTANSSPS TNLQWLSTLN TDDIPTKNYR KSSIIGTIGP NTNSAEMISK
60 70 80 90 100
LRQAGLNIVR MNFSHGSYEY HQSVIENARE SEQQFQGRPL AIALDTKGPE
110 120 130 140 150
IRTGVTKDDK DWDVKAGHVM LFSTNPKYKD QCDDKIMYID YTNIVKQIDI
160 170 180 190 200
GKIIFVDDGV LSFKVLEKID GETLKVETLN NGKISSRKGV NLPGTDVDLP
210 220 230 240 250
ALSEKDKADL KFGVEHGVDM IFASFVRTAN DVQAIRDVLG EKGKGIQVIS
260 270 280 290 300
KIENQQGVNN FDEILKETDG VMVARGDLGI EIPAPQVFIA QKQLIAKCNL
310 320 330 340 350
AGKPVICATQ MLDSMTYNPR PTRAEVSDVG NAVLDGADCV MLSGETAKGT
360 370 380 390 400
YPIESVKMMH ETCLVAEKAI AYAPLFNEMR TLTVRPTETV ETIAISAVSA
410 420 430 440 450
SFEQQARAII VLSTSGTSAR LCSKYRPNCP ILMVTRNAQA ARFSHLYRGV
460 470 480 490 500
YPFIYHKARA SNPAEWQHDV EERLKWGMDE AVALGILNKG DVVVAIQGWT
510
GGLGHTNTLR VLECV
Length:515
Mass (Da):56,668
Last modified:October 11, 2004 - v3
Checksum:iED3E51220306F1D6
GO

Sequence cautioni

The sequence AAA18520 differs from that shown. Reason: Frameshift at position 504.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti84 – 86QFQ → RFR in AAA18520 (PubMed:1511886).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86863 Unassigned DNA Translation: AAA18520.1 Frameshift.
CR382132 Genomic DNA Translation: CAG78002.2
PIRiJC1267
RefSeqiXP_505195.2, XM_505195.2

Genome annotation databases

EnsemblFungiiCAG78002; CAG78002; YALI0_F09185g
GeneIDi2907873
KEGGiyli:YALI0F09185g

Similar proteinsi

Entry informationi

Entry nameiKPYK_YARLI
AccessioniPrimary (citable) accession number: P30614
Secondary accession number(s): Q6C2B7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: October 11, 2004
Last modified: May 23, 2018
This is version 127 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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