P30602 (CHS3_EXODE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chitin synthase 3
EC=2.4.1.16

Alternative name(s):
Chitin-UDP acetyl-glucosaminyl transferase 3
Class-III chitin synthase 3

Gene names

Name:

CHS3

Organism

Exophiala dermatitidis (Wangiella dermatitidis)

Taxonomic identifier

5970 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Chaetothyriomycetidae › Chaetothyriales › Herpotrichiellaceae › mitosporic Herpotrichiellaceae › Exophiala

Protein attributes

Sequence length	885 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays a major role in cell wall biogenesis.
Catalytic activity	UDP-N-acetyl-D-glucosamine + (1,4-(N-acetyl-beta-D-glucosaminyl))(n) = UDP + (1,4-(N-acetyl-beta-D-glucosaminyl))(n+1).
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the chitin synthase family. Class III subfamily.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW chitin biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	chitin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 885	885	Chitin synthase 3		PRO_0000193696
Regions
	Transmembrane	565 – 585	21	Helical; Potential
	Transmembrane	620 – 640	21	Helical; Potential
	Transmembrane	650 – 670	21	Helical; Potential
	Transmembrane	707 – 727	21	Helical; Potential
	Transmembrane	735 – 755	21	Helical; Potential
	Transmembrane	837 – 857	21	Helical; Potential
Experimental info
	Sequence conflict	215	1	Y → M in AAA30336. Ref.2
	Sequence conflict	340	1	R → S in AAA30336. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P30602 [UniParc]. Last modified July 15, 1999. Version 2. Checksum: 48D00BEE408398F0	FASTA	885	99,423
	10 20 30 40 50 60 MASQYPGHQL DDIPSTNVYR PPPRHEDDEA EHALLHQNSA YQSQYDDPHS RPLTPGQESV 70 80 90 100 110 120 YTLNESYVGG DPSKVPVTSY NPQYTQPYGQ GYGMNNSRPG FPTPGPPDPI DRTDSTEAWR 130 140 150 160 170 180 ERQAPGFGTI KRYATRKVKL VQGSVLSIDY PVPSAIQNAI QAKYRNDLEG GSEEFTHMRY 190 200 210 220 230 240 TAATCDPDDF TLKNGYNLRP AMYNRHTELL IAITYYNEDK VLTARTLHGV MQNIRDIVNL 250 260 270 280 290 300 KKSEFWNKGG PAWQKIVVCL VFDGIDPCDK NTLDVLATIG VYQDGVMKKD VDGKETVAHI 310 320 330 340 350 360 FEYTTQLSVT ANQQLIRPND NDATSLPPAQ MIFCLKQKNR KKINSHRWLF NAFGRILNPE 370 380 390 400 410 420 VCILLDAGTK PGSKSLMALW QAFYNDKDLG GACGEIHAML GPGGVFGRKL LNPLVAAQNF 430 440 450 460 470 480 EYKISNILDK PLESSFGYVS VLPGAFSAYR FRAIMGRPLE QYFHGDHTLS KTLGKKGIEG 490 500 510 520 530 540 MNIFKKNMFL AEDRILCFEL VAKAGSKWHL SYVKASKAET DVPEGPPEFI GQRRRWLNGS 550 560 570 580 590 600 FAASMYCLMH FSRMYKSGHN LIRMFFLHIQ MIYNIVSVLL SWFSLASFWL TTKVLMDLVG 610 620 630 640 650 660 QPSTSNDNAA FPFGNTATPI INTILQYLYL AFLLLQFILA LGNRPKGSKV AYIISFCLFG 670 680 690 700 710 720 LIQLYVIVLS MYLVVRAFTT KNGTDIVTNE GANEFVKSFF ASTGPGIVII ALAATFGLYF 730 740 750 760 770 780 VASFLYMDPW HMFTSFAQYL LLMPSFINIL MIYAFSNWHD VSWGTKGSDK ADVLPSAQTK 790 800 810 820 830 840 KDEKSKTAVV EEVDKPQADI DSQFEATVRR ALAPYKPPEE KEEKTLEDSY KNFRTRLVAT 850 860 870 880 WIFSNALLAV AITSDSLDRF GFTSEPLRGP AISSRRFCGL LRPCL

References

[1]	"Characterization and expression studies of WdCHS3, a gene that encodes a class III chitin synthase and contributes to virulence in Wangiella (Exophiala) dermatitidis." Wang Z., Graybill J.R., Szaniszlo P.J. Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 8656.
[2]	"Classification of fungal chitin synthases." Bowen A.R., Chen-Wu J.L.-P., Momany M., Young R., Szaniszlo P.J., Robbins P.W. Proc. Natl. Acad. Sci. U.S.A. 89:519-523(1992) [PubMed: 1731323] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 214-410.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF053314 Genomic DNA. Translation: AAC35278.1. M81907 Genomic DNA. Translation: AAA30336.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR004834. Chitin_synth. IPR004835. Chitin_synth_fng. IPR013616. Chitin_synth_N. [Graphical view]
Pfam	PF01644. Chitin_synth_1. 1 hit. PF08407. Chitin_synth_1N. 1 hit. PF03142. Chitin_synth_2. 1 hit. [Graphical view]
ProDom	PD002998. Chitin_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

CHS3_EXODE

Accession

Primary (citable) accession number: P30602
Secondary accession number(s): O74678

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	July 15, 1999
Last modified:	May 31, 2011
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program