P30601 (CHS2_EXODE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chitin synthase 2
EC=2.4.1.16

Alternative name(s):
Chitin-UDP acetyl-glucosaminyl transferase 2
Class-I chitin synthase 2

Gene names

Name:

CHS2

Organism

Exophiala dermatitidis (Wangiella dermatitidis)

Taxonomic identifier

5970 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Chaetothyriomycetidae › Chaetothyriales › Herpotrichiellaceae › mitosporic Herpotrichiellaceae › Exophiala

Protein attributes

Sequence length	928 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays a major role in cell wall biogenesis.
Catalytic activity	UDP-N-acetyl-D-glucosamine + (1,4-(N-acetyl-beta-D-glucosaminyl))(n) = UDP + (1,4-(N-acetyl-beta-D-glucosaminyl))(n+1).
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the chitin synthase family. Class I subfamily.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW chitin biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	chitin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 928	928	Chitin synthase 2		PRO_0000193695
Regions
	Transmembrane	472 – 492	21	Helical; Potential
	Transmembrane	570 – 589	20	Helical; Potential
	Transmembrane	613 – 633	21	Helical; Potential
	Transmembrane	644 – 664	21	Helical; Potential
	Transmembrane	678 – 698	21	Helical; Potential
	Transmembrane	723 – 743	21	Helical; Potential
	Transmembrane	753 – 773	21	Helical; Potential
	Transmembrane	854 – 874	21	Helical; Potential
	Transmembrane	893 – 913	21	Helical; Potential
Experimental info
	Sequence conflict	259 – 260	2	VV → KL in AAA30335. Ref.2
	Sequence conflict	462	1	M → L in AAA30335. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P30601 [UniParc]. Last modified July 15, 1999. Version 2. Checksum: 001AF053F8873C9B	FASTA	928	105,713
	10 20 30 40 50 60 MAYNRLGSPQ RDGPYSPSAQ PQYDSRSPSP GRPLQPYIHP DEAYARQQPL HLQMPTASDD 70 80 90 100 110 120 RLAMQPTYSV ENVHNPQAYG QQYGQHLPDS GDMGYGRNDY IVSPEEHHDA YYTQPYSPHP 130 140 150 160 170 180 QGDYALDPYP SHDEPYRPDT DNVPILQPDS AYGPDPHTQP GMDYDDYQEE PRPTPSPAPI 190 200 210 220 230 240 RRWKTVKEVQ LFNGNLVLDC PVPPKLLANV PHAKPPERDE FTHMRYSAAT CDPSDFHNER 250 260 270 280 290 300 FTLRQRLFAK PRQTELFIVV TMYNEDEFLF ARTMIGVFKN IEFMCNRSSS KTWGKEAWKK 310 320 330 340 350 360 IVVCIVSDGR AKINPRTRAV LAGLGVYQDG IAKQQVNGKD VTAHIYEYTT QVGLELKGTQ 370 380 390 400 410 420 VSLKPRSATP VQLLFCLKEK NQKKINSHRW FFQAFGRVLD PNICVLIDAG TKPGKDSIYQ 430 440 450 460 470 480 LWKAFDLEPM CGGACGEIKV MLDHGKKLLN PLVATQNFEY KMSNILDKPL ESAFGFISVL 490 500 510 520 530 540 PGAFCAYRYV ALQNDKNGVG PLEKYFKGET MHADAGVFTA NMYLAEDRIL CFELVSKRNC 550 560 570 580 590 600 RWILQYVKSA TGETDVPDRI PEFVLQRRRW LNGSFFAAVY AVAHVYQLWR TDHSFLRKLM 610 620 630 640 650 660 FLIEFTYQTI NMLFAWFAIG NFFLVFRLLT ASLGTKETLG TAGTVLGVVF EFVYLGTLLY 670 680 690 700 710 720 CFILSMGNRP QGNPKSYMMM VIFWSVLMVW LTFASIFLTV KSIETEVQQK DFSFSTIFNN 730 740 750 760 770 780 STFFGLIVSL ASTYVLWFVA SFLFFDPWHM FTCFLQYIVL TPTYINVLNI YAFCNTHDIT 790 800 810 820 830 840 WGTKGDDKAE KLPSANVKPG GKVDVLIPQD DGDLNAQYDS ELKKFATKPP KEVKAPNPAD 850 860 870 880 890 900 KQEDYYKSFR SNVVTAWMIT NFILVAAVLN IAGFDRINVH DTQQQNSTIY LAVILWSVAG 910 920 LSLFRFTGAC WFLVVRMVSL EIWSVCKV

References

[1]	"Cloning and characterization of WdCHS2, a class I chitin synthase gene, in Wangiella (Exophiala) dermatitidis." Zheng L., Szaniszlo P.J. Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 8656.
[2]	"Classification of fungal chitin synthases." Bowen A.R., Chen-Wu J.L.-P., Momany M., Young R., Szaniszlo P.J., Robbins P.W. Proc. Natl. Acad. Sci. U.S.A. 89:519-523(1992) [PubMed: 1731323] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 259-462.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF052606 Genomic DNA. Translation: AAC34496.1. M81906 Genomic DNA. Translation: AAA30335.1.
PIR	A45188.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR004834. Chitin_synth. IPR013616. Chitin_synth_N. [Graphical view]
Pfam	PF01644. Chitin_synth_1. 1 hit. PF08407. Chitin_synth_1N. 1 hit. [Graphical view]
ProDom	PD002998. Chitin_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

CHS2_EXODE

Accession

Primary (citable) accession number: P30601
Secondary accession number(s): O74210

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	July 15, 1999
Last modified:	May 31, 2011
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program