P30600 (CHS1_EXODE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chitin synthase 1
EC=2.4.1.16

Alternative name(s):
Chitin-UDP acetyl-glucosaminyl transferase 1
Class-II chitin synthase 1

Gene names

Name:

CHS1

Organism

Exophiala dermatitidis (Wangiella dermatitidis)

Taxonomic identifier

5970 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Chaetothyriomycetidae › Chaetothyriales › Herpotrichiellaceae › mitosporic Herpotrichiellaceae › Exophiala

Protein attributes

Sequence length	988 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays a major role in cell wall biogenesis.
Catalytic activity	UDP-N-acetyl-D-glucosamine + (1,4-(N-acetyl-beta-D-glucosaminyl))(n) = UDP + (1,4-(N-acetyl-beta-D-glucosaminyl))(n+1).
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the chitin synthase family. Class II subfamily.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW chitin biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	chitin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 988	988	Chitin synthase 1		PRO_0000193694
Regions
	Transmembrane	577 – 596	20	Helical; Potential
	Transmembrane	616 – 636	21	Helical; Potential
	Transmembrane	656 – 676	21	Helical; Potential
	Transmembrane	732 – 752	21	Helical; Potential
	Transmembrane	764 – 784	21	Helical; Potential
	Transmembrane	864 – 884	21	Helical; Potential
	Transmembrane	911 – 931	21	Helical; Potential
Experimental info
	Sequence conflict	265 – 266	2	CV → KL in AAA30334. Ref.2
	Sequence conflict	468	1	I → L in AAA30334. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P30600 [UniParc]. Last modified July 15, 1999. Version 2. Checksum: 5940234B344F88A4	FASTA	988	111,221
	10 20 30 40 50 60 MVPFWTVVTD QHIKYTSHNK AGFIIDEEQH HWPPSSGSSL GRAPSIPLSS SNPRSPIRPS 70 80 90 100 110 120 TPSRVSTDWT RPPAPSVAYE ARDINGSPRL EPHHHNMEEV LEGLCHRAAL FGQGGCGRND 130 140 150 160 170 180 NPNARAGVGK RCLCRSLLDN AGQRPKGKLE IGTFVHDCLD LYGRWCHYQS EVESLWPSAG 190 200 210 220 230 240 RKTRYARPSR STNDQEGGYV DQWRADSRMY IPTILHSFLP RRDEREFTHM RYTAVTCDPD 250 260 270 280 290 300 DFVVKGYKLR QNIGPTMRET ELFICVTMYN EDEIEFTRTM HGIMRNIAHF CSRTRSRTWG 310 320 330 340 350 360 KDGWQKIVVC VIADGRQKVH PRTLNALAAM GVYQDGIAKN VVNQKEVTAH VYEYTTQVSL 370 380 390 400 410 420 DETLKFKGAE KGIVPCQMIF CLKEKNKKKL NSHRWFFNAF GRALIPNVCI LLDVGTKPDS 430 440 450 460 470 480 KALYHLWKAF DQNSNVAGAA GEIKADKGKG WLGLLNPLVA SQNFEYKISN ILDKPLESVF 490 500 510 520 530 540 GYITVLPGAL SAYRYHALQN DPSGHGPLSQ YFKGETLHGR DADVFTANMY LAEDRILCWE 550 560 570 580 590 600 LVAKRDEQWV LKFVKSAYGE TDVPDTVPEF ISQRRRWLNG AFFAAVYALV HFKQIWRTDH 610 620 630 640 650 660 SLTRKILLHI EFIYQFISLL FTFFSLANFY LTFYFVAGSL ADPTIDPFGH NIGKYIFVIL 670 680 690 700 710 720 RYVCVLLICL QFILSLGNRP QGEEIVSEYH GDVFHHHGIH DLCLRVYCHQ ATYDARSRKD 730 740 750 760 770 780 RPQQSYKLGN NIFTNLIVSS VSTIGLFFLM SFLYLDPWHM FTSSAQYFAL LPSYICTLQV 790 800 810 820 830 840 YAFCNTHDVT WGTKGDNVMH TDLGAAKAIG SGNTVEVEMP SEQLDIDSAY DVALRNLRDR 850 860 870 880 890 900 VEVPKPPVSE NQLQEDYYKS VRTYVVASYM VCNAILAMAV SEAYPVGSHI GSNFYLTFIL 910 920 930 940 950 960 WSVAALALFR AIGSSAFGVI NIVSAIAEGR IQAKFERIFG GGDERGRHRA GLGSGFSESG 970 980 KTGITSGSGM SGMSLSDVTS KISEKLAG

References

[1]	"Characterization of WdCHS1, a class II chitin synthase gene, and multiple wdchs mutants of Wangiella dermatitidis." Zheng L., Mendoza A.L., Wang Z., Szaniszlo P.J. Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 8656.
[2]	"Classification of fungal chitin synthases." Bowen A.R., Chen-Wu J.L.-P., Momany M., Young R., Szaniszlo P.J., Robbins P.W. Proc. Natl. Acad. Sci. U.S.A. 89:519-523(1992) [PubMed: 1731323] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 265-468.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF054503 Genomic DNA. Translation: AAC36064.1. M81905 Genomic DNA. Translation: AAA30334.1.
PIR	E45189.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BRENDA	2.4.1.16. 6682.
Family and domain databases
InterPro	IPR004834. Chitin_synth. IPR013616. Chitin_synth_N. [Graphical view]
Pfam	PF01644. Chitin_synth_1. 1 hit. PF08407. Chitin_synth_1N. 1 hit. [Graphical view]
ProDom	PD002998. Chitin_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

CHS1_EXODE

Accession

Primary (citable) accession number: P30600
Secondary accession number(s): O74681

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	July 15, 1999
Last modified:	May 31, 2011
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program