Reviewed, UniProtKB/Swiss-Prot P30599 (CHS2_USTMA)

Names and origin

Protein names

Recommended name:
    Chitin synthase 2
    EC=2.4.1.16
Alternative name(s):
    Chitin-UDP acetyl-glucosaminyl transferase 2

Gene names

Name:	CHS2
ORF Names:	UM04290

Organism

Ustilago maydis (Smut fungus) [Complete proteome]

Taxonomic identifier

5270 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Ustilaginomycotina › Ustilaginomycetes › Ustilaginales › Ustilaginaceae › Ustilago

Protein attributes

Sequence length	1074 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Plays a major role in cell wall biogenesis.
Catalytic activity	UDP-N-acetyl-D-glucosamine + (1,4-(N-acetyl-beta-D-glucosaminyl))(n) = UDP + (1,4-(N-acetyl-beta-D-glucosaminyl))(n+1).
Subcellular location	Cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein. Note: A constitutive cytoplasmic pool is present that localizes to intracellular microvesicles termed chitosomes. Chitosomes constitute a separate secretory route distinct from the typical secretory pathway and serve as a vehicle for delivering the enzyme to the sites on the cell surface where polysaccharide sythesis takes place By similarity. Localizes to septa of yeast-like cells and to the basal septum separating the living tip cell from the vacuolated part in hyphae.
Sequence similarities	Belongs to the chitin synthase family. Class II subfamily.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell membrane Cytoplasmic vesicle Membrane
Domain	Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW chitin biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasmic vesicle Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	chitin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1074	1074	Chitin synthase 2		PRO_0000193722
Regions
	Transmembrane	608 – 628	21	Potential
	Transmembrane	742 – 762	21	Potential
	Transmembrane	779 – 799	21	Potential
	Transmembrane	817 – 837	21	Potential
	Transmembrane	867 – 887	21	Potential
	Transmembrane	891 – 911	21	Potential
	Transmembrane	1001 – 1021	21	Potential
	Transmembrane	1048 – 1068	21	Potential
Amino acid modifications
	Glycosylation	72	1	N-linked (GlcNAc...) Potential
	Glycosylation	97	1	N-linked (GlcNAc...) Potential
	Glycosylation	149	1	N-linked (GlcNAc...) Potential
	Glycosylation	289	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P30599-1 [UniParc]. Last modified August 30, 2005. Version 2. Checksum: 1EDA92BD08837863	FASTA	1,074	120,446
	10 20 30 40 50 60 MSHYHRQGGP GQPHDSYEDQ QQPYYTDQAH SGYDHHSYHQ QQLYHAAYDA AQPEYQAAPV 70 80 90 100 110 120 KPQRQPSRIR SNSSGSRSVS HTPAAYTNQG IPPVPSNLSA ARQRSDPSQA LPPSSSSYAQ 130 140 150 160 170 180 DAFSRPSYSS HRNAPNAPNS NHPSRWDPNA SYDTAPTAPL YDPAPLPGSG QLDADPSQHS 190 200 210 220 230 240 IDLGVREPLY DDQVMQQHYP YGGAAAFQRS DSYQSGRPGA GGYHSIDDEK SIHSKHSNQP 250 260 270 280 290 300 PGAWNAGAPS MPYNNMPTSH STIQMAQPAY PPSPYGEYEM HATGMPEPNM SIAGLGAPGA 310 320 330 340 350 360 LIAAAPMPGM QHHDSTYSRE NRERIMRKRT VKRIPLQDGN LVLDIPVASS ISKSTTNNPE 370 380 390 400 410 420 FREMRYQACT SDPDRFIEEK YTLRPWLYGR ETEMAIVLTC YNEDDVLFAR TMGGVIKNIA 430 440 450 460 470 480 HLCSRTRSKT WGPDAWKKVV VIIVADGRKK ANERMLKALG LMGCYNEGVM KDHVLKKPVE 490 500 510 520 530 540 AHIFEYTTRV QITEKGEVKV TPCPIQVVFC LKEQNKKKLN SHRWYFNAFC QMLKPNVCIL 550 560 570 580 590 600 LDVGTKPTGT SIYELWKSFD KHHRVGGACG EICVDTGRGC TALFNPLVAS QNFEYKMSNI 610 620 630 640 650 660 LDKPTESVFG FISVLPGAFS AYRYKALLGR PLEMYFKGEK LHSGEGSNSI FEGNMYLAED 670 680 690 700 710 720 RILCFELVTK EREGWLLRYV KSAKAYTDVP DRVPEFISQR RRWLNGSLFA SYYAVWHWYR 730 740 750 760 770 780 IFTSGQPFLR KLWLLFQVIY NLVLLVFSWF GIANFFLAFY FLLSASTSTE GSDPFGGQGA 790 800 810 820 830 840 AIVEIFQNIF IAMVIVVLVC SLGNRPQGSN FAYTSAIIIF ALIMGLALYA AGYTIYLALD 850 860 870 880 890 900 AAGLTHTNGW RVDNLETLFK TSGFRDIVIS LAATYVMWLL CSLLHLEPWH MLTSFVQYLF 910 920 930 940 950 960 LTPTYVIILS MYSMCNTNDL SWGTKQSNGP ATDLGGATGC NSKQDGKGEM VDVKIPTSAA 970 980 990 1000 1010 1020 DAEELWTHYR QTLSQPTVEV KQKRDKATRQ EDHAKNFRTN LVLIWMCTNA LVVIIFTSTW 1030 1040 1050 1060 1070 WNKYVRNHIY AGAVRRGEPV INPYQSAIFW STAGLSAVRF VGSITFLLLR LFGH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis." Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J., Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H., Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G., Snetselaar K., McCann M., Perez-Martin J. , Feldbruegge M., Basse C.W., Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L., Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L., Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N., Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B., Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J., Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P., Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G., Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A., Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M., Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M., Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E., Birren B.W. Nature 444:97-101(2006) [PubMed: 17080091] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 521.
[2]	"Classification of fungal chitin synthases." Bowen A.R., Chen-Wu J.L.-P., Momany M., Young R., Szaniszlo P.J., Robbins P.W. Proc. Natl. Acad. Sci. U.S.A. 89:519-523(1992) [PubMed: 1731323] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 405-590.
[3]	"Polar localizing class V myosin chitin synthases are essential during early plant infection in the plant pathogenic fungus Ustilago maydis." Weber I., Assmann D., Thines E., Steinberg G. Plant Cell 18:225-242(2006) [PubMed: 16314447] [Abstract] Cited for: SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AACP01000150 Genomic DNA. Translation: EAK85339.1. M82959 Genomic DNA. Translation: AAA34225.1.
PIR	A45189.
RefSeq	XP_760437.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Genome annotation databases
GeneID	3632333.
KEGG	uma:UM04290.1.
Phylogenomic databases
OMA	CLKEQNK.
Enzyme and pathway databases
BRENDA	2.4.1.16. 2320.
Family and domain databases
InterPro	IPR004834. Chitin_synth. IPR004835. Chitin_synth_fng. IPR013616. Chitin_synth_N. [Graphical view]
Pfam	PF01644. Chitin_synth_1. 1 hit. PF08407. Chitin_synth_1N. 1 hit. PF03142. Chitin_synth_2. 1 hit. [Graphical view]
ProDom	PD002998. Chitin_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

CHS2_USTMA

Accession

Primary (citable) accession number: P30599
Secondary accession number(s): Q4P6H3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	August 30, 2005
Last modified:	November 3, 2009
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)