P30598 (CHS1_USTMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chitin synthase 1
EC=2.4.1.16

Alternative name(s):
Chitin-UDP acetyl-glucosaminyl transferase 1

Gene names

Name:	CHS1
ORF Names:	UM10718, UM05637

Organism

Ustilago maydis (strain 521 / FGSC 9021) (Smut fungus)

Taxonomic identifier

237631 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Ustilaginomycotina › Ustilaginomycetes › Ustilaginales › Ustilaginaceae › Ustilago

Protein attributes

Sequence length	939 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays a major role in cell wall biogenesis.
Catalytic activity	UDP-N-acetyl-D-glucosamine + (1,4-(N-acetyl-beta-D-glucosaminyl))(n) = UDP + (1,4-(N-acetyl-beta-D-glucosaminyl))(n+1).
Subcellular location	Cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein. Note: A constitutive cytoplasmic pool is present that localizes to intracellular microvesicles termed chitosomes. Chitosomes constitute a separate secretory route distinct from the typical secretory pathway and serve as a vehicle for delivering the enzyme to the sites on the cell surface where polysaccharide sythesis takes place By similarity. Localizes to septa of yeast-like cells and to the basal septum separating the living tip cell from the vacuolated part in hyphae. Ref.3
Sequence similarities	Belongs to the chitin synthase family. Class III subfamily.
Sequence caution	The sequence EAK86040.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell membrane Cytoplasmic vesicle Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW chitin biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasmic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	chitin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 939	939	Chitin synthase 1		PRO_0000193721
Regions
	Transmembrane	614 – 634	21	Helical; Potential
	Transmembrane	650 – 670	21	Helical; Potential
	Transmembrane	688 – 708	21	Helical; Potential
	Transmembrane	733 – 753	21	Helical; Potential
	Transmembrane	762 – 782	21	Helical; Potential
	Transmembrane	863 – 883	21	Helical; Potential
	Transmembrane	907 – 927	21	Helical; Potential
Amino acid modifications
	Glycosylation	207	1	N-linked (GlcNAc...) Potential
	Glycosylation	576	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	312	1	K → E in AAA34224. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P30598 [UniParc]. Last modified January 9, 2007. Version 2. Checksum: 4C9C253135EBFFC5	FASTA	939	106,001
	10 20 30 40 50 60 MSYDAYQMRP GQGRDYARQQ RQQRSYQLSP WRDLAGSMRV ALQRVRPLQD DPLRQPGAPY 70 80 90 100 110 120 ANPAGGYPSS ASMNPENPFY AAENPSFQTL APATSEGHAT YTYEEDKIPL TDSADEKYGF 130 140 150 160 170 180 SQNGHSTYNL ASQSGFPPST PCGGPSSYSS ALGPEDSASQ VAWAKRQQAP KRGLTKKIQL 190 200 210 220 230 240 TRGHWIVDHP VPTAVKNSVE SRWSQGNRTQ EFTHMRYTAA TCDPDEFTLE NGWSLRTSQQ 250 260 270 280 290 300 YGRDTELLIA ITYYNEDRIL LARTLHGVML NIRDICKSKS SKFWRRSAEE GRPGWQRIVV 310 320 330 340 350 360 SLIFDGIDPC DKEVLDLLAT VGVYQDGVMK RKVDGKDTVA HLFEYTTQLS VDPTPALIQP 370 380 390 400 410 420 HADDASNLVP VQMIFCLKQK NSKKINSHRW LFNALGRHLQ PELCVLIDAG TKPGHKSLYY 430 440 450 460 470 480 LWEAFYNNAN LGGACGEIHA MIKNGRKLIN PLVAAQNFEY KMSNILDKPL ESTFGYVSVL 490 500 510 520 530 540 PGAFSAYRFR AIQGRPLQQY FHGDHTLADR LGKKGLHGMD IFTKNMFLAE DRILCFELVA 550 560 570 580 590 600 KAGDKWTLTY VKPSKGETDV PEGAAELISQ RRRWLNGSFA ASIYSLVHFF RIYKSNHGII 610 620 630 640 650 660 RLFFLHIQAL YNAIVLLFSW FALANLWLTF SIIIEFLPDE LLKNSSHTTL VVFHWINQAA 670 680 690 700 710 720 KWIYVFFLVL QFVLALGNRP KGEKPTYIAS FIVFGILGLY LIFVSLWLTL KALLETSVSG 730 740 750 760 770 780 NIWHTLFNQT TGVLIAALAA TFGIYLIASI LYADPWHMVT SFPQYMMIAP SFINILNVYA 790 800 810 820 830 840 FCNLHDVSWG TKGSDKADAL PTVDTKKDKS TEPGTVEEIE RHQDDIDETF KAVVSRAVAP 850 860 870 880 890 900 FKPAETVEKP TMDDSNKTFR TRLVAFWLLT NGALTVAIEN VNGLNTGLTN KQIEQQQSSK 910 920 930 QSTYFRIILW ATFGLSAFRF IGCLIYWVKR NSTRCFRKT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis." Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J., Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H., Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G., Snetselaar K., McCann M., Perez-Martin J. , Feldbruegge M., Basse C.W., Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L., Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L., Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N., Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B., Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J., Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P., Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G., Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A., Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M., Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M., Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E., Birren B.W. Nature 444:97-101(2006) [PubMed: 17080091] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 521 / FGSC 9021.
[2]	"Classification of fungal chitin synthases." Bowen A.R., Chen-Wu J.L.-P., Momany M., Young R., Szaniszlo P.J., Robbins P.W. Proc. Natl. Acad. Sci. U.S.A. 89:519-523(1992) [PubMed: 1731323] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 258-455.
[3]	"Polar localizing class V myosin chitin synthases are essential during early plant infection in the plant pathogenic fungus Ustilago maydis." Weber I., Assmann D., Thines E., Steinberg G. Plant Cell 18:225-242(2006) [PubMed: 16314447] [Abstract] Cited for: SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AACP01000206 Genomic DNA. Translation: EAK86040.1. Sequence problems. M82958 Genomic DNA. Translation: AAA34224.1.
PIR	F45189.
RefSeq	XP_761784.1. XM_756691.1.
3D structure databases
ProteinModelPortal	P30598.
ModBase	Search...
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3633820.
KEGG	uma:UM05637.1.
Phylogenomic databases
eggNOG	fuNOG04531.
HOGENOM	HBG327157.
OrthoDB	EOG405W8B.
Enzyme and pathway databases
BRENDA	2.4.1.16. 6587.
Family and domain databases
InterPro	IPR004834. Chitin_synth. IPR013616. Chitin_synth_N. [Graphical view]
KO	K00698.
Pfam	PF01644. Chitin_synth_1. 1 hit. PF08407. Chitin_synth_1N. 1 hit. [Graphical view]
ProDom	PD002998. Chitin_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

CHS1_USTMA

Accession

Primary (citable) accession number: P30598
Secondary accession number(s): Q4P2M6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	January 9, 2007
Last modified:	December 14, 2011
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program