Reviewed, UniProtKB/Swiss-Prot P30597 (CHS1_SCHPO)

Names and origin

Protein names

Recommended name:
    Chitin synthase 1
    EC=2.4.1.16
Alternative name(s):
    Chitin-UDP acetyl-glucosaminyl transferase 1

Gene names

Name:	chs1
ORF Names:	SPAC13G6.12c, SPAC24B11.01c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	859 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Plays a major role in cell wall biogenesis.
Catalytic activity	UDP-N-acetyl-D-glucosamine + (1,4-(N-acetyl-beta-D-glucosaminyl))(n) = UDP + (1,4-(N-acetyl-beta-D-glucosaminyl))(n+1).
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the chitin synthase family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ascospore wall assembly Inferred from mutant phenotype. Source: GeneDB_SPombe ascospore wall chitin biosynthetic process Inferred from direct assay. Source: GeneDB_SPombe
Cellular component	endoplasmic reticulum Inferred from direct assay. Source: GeneDB_SPombe integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	chitin synthase activity Inferred from direct assay. Source: GeneDB_SPombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 859	859	Chitin synthase 1		PRO_0000193716
Regions
	Transmembrane	544 – 564	21	Potential
	Transmembrane	615 – 635	21	Potential
	Transmembrane	662 – 682	21	Potential
	Transmembrane	793 – 813	21	Potential
	Transmembrane	833 – 853	21	Potential
Experimental info
	Sequence conflict	273	1	K → R in AAA35297. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P30597-1 [UniParc]. Last modified November 1, 1995. Version 2. Checksum: 63443713A7ADE421	FASTA	859	97,990
	10 20 30 40 50 60 MRRWFKKTLP RPPDEEESAG LTNKDIVETN HLYPTITNLS LNSDTSSILF DKNKKPLKPK 70 80 90 100 110 120 IIIPDKEFDL DYYLKDETES IQSPFEGFTA QPNFSNFNKQ GNTMNREANF QRTNEKIQRN 130 140 150 160 170 180 KSIKRVKLFH GNLILDCPIP KKLLVTLPQQ TEREFAYMRY SAATCDPQDF SKSLFTLRQP 190 200 210 220 230 240 LFFQPRKTEI CIAITMYNED EVLFARTMHS VMKNISHLCT RKNSQVWGKD AWKKVVVCII 250 260 270 280 290 300 SDGRTKIHPR TLAYLAAIGV YQDGIAKNQV NDKEVKAHIY EYTTQLSIDP NLKFKGSDRG 310 320 330 340 350 360 IVPVQMIFCL KEKNQKKLNS HLWFFQAFCP ILKPEVCILL DAGTRPGDQS IYHLWKSFDL 370 380 390 400 410 420 NPQVAGACGE IVVMKGKLGS GLINPLVATQ NFEYKMSNIL DKPVESVFGF ISVLPGAFSA 430 440 450 460 470 480 YRFEALQNDS QGNGPLASYF KGELQNTGKS GIFEANMYLA EDRILCFELV SKKNEAWILH 490 500 510 520 530 540 YVKSAYADTD VPDRIPEFVL QRRRWLNGSF FAAAYAICHY YRFFRTSHTI SRKFMLSIEF 550 560 570 580 590 600 IYQLATIVFG WFNIGNFFII FYILTSSLAS TSANFLPGEI LFRIAIWIYA SLLVTCFVLA 610 620 630 640 650 660 LGNRPHGSPN FYLSMVIMYS ILMGYLLFCS GWIAYRAISD AIHNASSTSS SYTSALLNSN 670 680 690 700 710 720 VFINIVISLS STYGMYLVVS IISFDPWHMF TSFVQYIFLS IMYTNVLNVY AFCNTHDVSW 730 740 750 760 770 780 GTKGDHFTNN DLGVARLLQK GADVEIAIPT NQSDIDAKYE DAVKLLASPS LEFNSPIINH 790 800 810 820 830 840 GEQEDFYKNF RTYVVLTWIL SNLFLVGIVL SIPKINGISI SNNETSAYLS FLLWSVVAFS 850 VFRFIGCIFY LFIRLCTGE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[2]	"Classification of fungal chitin synthases." Bowen A.R., Chen-Wu J.L.-P., Momany M., Young R., Szaniszlo P.J., Robbins P.W. Proc. Natl. Acad. Sci. U.S.A. 89:519-523(1992) [PubMed: 1731323] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 201-389.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329670 Genomic DNA. Translation: CAA91105.1. M82957 Genomic DNA. Translation: AAA35297.1.
PIR	S62441.
RefSeq	NP_592838.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P30597.
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Genome annotation databases
GeneID	2542858.
GenomeReviews	Gene locus chs1 in contig CU329670_GR.
KEGG	spo:SPAC13G6.12c.
NMPDR	fig|4896.1.peg.2808.
Organism-specific databases
GeneDB_Spombe	SPAC13G6.12c.
Phylogenomic databases
eggNOG	fuNOG04531.
HOGENOM	HBG327157.
OMA	TMYNEDD.
OrthoDB	EOG9VMGXR.
PhylomeDB	P30597.
Enzyme and pathway databases
BRENDA	2.4.1.16. 653.
Gene expression databases
ArrayExpress	P30597.
Family and domain databases
InterPro	IPR004834. Chitin_synth. IPR013616. Chitin_synth_N. [Graphical view]
Pfam	PF01644. Chitin_synth_1. 1 hit. PF08407. Chitin_synth_1N. 1 hit. [Graphical view]
ProDom	PD002998. Chitin_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

CHS1_SCHPO

Accession

Primary (citable) accession number: P30597

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	November 1, 1995
Last modified:	January 19, 2010
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)