P30595 (CHS2_RHIOL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chitin synthase 2
EC=2.4.1.16

Alternative name(s):
Chitin-UDP acetyl-glucosaminyl transferase 2

Gene names

Name:

CHS2

Organism

Rhizopus oligosporus

Taxonomic identifier

4847 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Fungi incertae sedis › Early diverging fungal lineages › Mucoromycotina › Mucorales › Mucoraceae › Rhizopus › Rhizopus microsporus

Protein attributes

Sequence length	858 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays a major role in cell wall biogenesis.
Catalytic activity	UDP-N-acetyl-alpha-D-glucosamine + (1,4-(N-acetyl-beta-D-glucosaminyl))(n) = UDP + (1,4-(N-acetyl-beta-D-glucosaminyl))(n+1).
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the chitin synthase family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological_process	chitin biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	chitin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 858	858	Chitin synthase 2		PRO_0000193712
Regions
	Transmembrane	500 – 517	18	Helical; Potential
	Transmembrane	540 – 560	21	Helical; Potential
	Transmembrane	586 – 606	21	Helical; Potential
	Transmembrane	621 – 641	21	Helical; Potential
	Transmembrane	665 – 685	21	Helical; Potential
	Transmembrane	799 – 819	21	Helical; Potential
	Transmembrane	825 – 845	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P30595 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 5B2DECFF5823408C	FASTA	858	96,791
	10 20 30 40 50 60 MYPEGPKPEH DQFPLQDTQF SNQPPVHRSP FEDPYPEDQP HFDKQPLLTS PAYPPTQYPT 70 80 90 100 110 120 SPPPPNFPGS PAVQQPYPPF NNNPSPVSPG VPAYFNPAPP SPNMHYGQAP RRQPRRFKTT 130 140 150 160 170 180 RQVKLTKGNL VLDCPVPTAY LNDVPIKDGK EFTHMRYTAA TCDPKDFASD GYTLRQPMLG 190 200 210 220 230 240 RKTELFIVLT MYNEDEVLFA RTMHGVMKNI AHLCTRDRSR TWGPNGWEKV VVCIVSDGRN 250 260 270 280 290 300 KINQRTLSVL ALLGVYQDGI AKNVVHGKPV TAHIYEYTTQ VSVDPNMEVK KAGSKNVVPC 310 320 330 340 350 360 QILFCLKEKN QKKINSHRWF FQAFGPVIDP HICVLIDVGT KPGGTSIYHL WKAFDINSNI 370 380 390 400 410 420 AGACGEIRAM AGTRGVALLN PLVAAQNFEY KMSNILDKPL ESVFGYISVL PGAFSAYRFK 430 440 450 460 470 480 ALQNDVNGHG PLEKYFLGET QHGGDADIFT ANMYLAEDRI LCYELVAKKE AHWVLHYVSS 490 500 510 520 530 540 SYGETDVPDK VDEFISQRRR WLNGSFFAGV YALYHWRKVW QSDHSYLRKM MFMVEDIYNT 550 560 570 580 590 600 YNLIFSWFAL GNFYLTFFIL TKALGHGVDG STLTDPPFSP DTGETLHTVF NYIYIVLIVI 610 620 630 640 650 660 QFIMALGNRP QGSKIAYTSS MVFFAILMVY MMFAAIWITV VGVKTVVETS GGQFIEMLEQ 670 680 690 700 710 720 STFRNIIISL CATYVMYFVS SFMFLDPWHM FTSFIQYILL SPSYTNVLNI YAFCNTHDVS 730 740 750 760 770 780 WGTKGDNTVA TDLGVVKAKK DGSGDLAVEV EVPVEEKDIN AAFIDAQVEL TKKIEPEKSH 790 800 810 820 830 840 RDAKTKQEDY YRSFRTRLVL AWIISNLALV VAIANTTVID IKGKASIYLG FILWSVAGLS 850 VIRFTGSTLY LIFKIFTG

References

[1]	"Isolation and characterization of two chitin synthase genes of Rhizopus oligosporus." Motoyama T., Sudoh M., Horiuchi H., Ohta A., Takagi M. Biosci. Biotechnol. Biochem. 58:1685-1693(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	D10160 Genomic DNA. Translation: BAA01024.1.
PIR	JC2309.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR004834. Chitin_synth. IPR013616. Chitin_synth_N. [Graphical view]
Pfam	PF01644. Chitin_synth_1. 1 hit. PF08407. Chitin_synth_1N. 1 hit. [Graphical view]
ProDom	PD002998. Chitin_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

CHS2_RHIOL

Accession

Primary (citable) accession number: P30595

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	April 3, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program