P30594 (CHS1_RHIOL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chitin synthase 1
EC=2.4.1.16

Alternative name(s):
Chitin-UDP acetyl-glucosaminyl transferase 1

Gene names

Name:

CHS1

Organism

Rhizopus oligosporus

Taxonomic identifier

4847 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Fungi incertae sedis › Basal fungal lineages › Mucoromycotina › Mucorales › Mucoraceae › Rhizopus

Protein attributes

Sequence length	858 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays a major role in cell wall biogenesis.
Catalytic activity	UDP-N-acetyl-D-glucosamine + (1,4-(N-acetyl-beta-D-glucosaminyl))(n) = UDP + (1,4-(N-acetyl-beta-D-glucosaminyl))(n+1).
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the chitin synthase family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW chitin biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	chitin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 858	858	Chitin synthase 1		PRO_0000193711
Regions
	Transmembrane	537 – 557	21	Helical; Potential
	Transmembrane	576 – 596	21	Helical; Potential
	Transmembrane	609 – 629	21	Helical; Potential
	Transmembrane	661 – 680	20	Helical; Potential
	Transmembrane	685 – 707	23	Helical; Potential
	Transmembrane	791 – 811	21	Helical; Potential
	Transmembrane	831 – 851	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P30594 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: EE1E6197F00E70B9	FASTA	858	97,058
	10 20 30 40 50 60 MYPYNNSNNN SPMPSARLPS RPRYGGGEEY PMQQVSFNNN SPPPPPFPSS PNDTGFYDGN 70 80 90 100 110 120 DVQRPLLVNN GSTASSVTLN NGNGGNVRFT NAKSNMIGGG PDNVGIAPRK QTRRYKTIRR 130 140 150 160 170 180 VKLTGGNLVL DNPVPSKYLQ RVKRKDKEEF THMRYTAATC DPADFERENY KLRQNLLGRE 190 200 210 220 230 240 TELFIVLTMY NEDEVLFCRT MNGVMKNVSH LCSRNRSSTW GPEGWKKVVV CIVSDGRTKI 250 260 270 280 290 300 HPRTLSVLAA MGVYQEGMAK NIVEGKPVTA HIYEHTTQLS IDADMNFRGD EKGVVPVQIL 310 320 330 340 350 360 FCLKEKNQKK INSHRWFFQA FGPLLKPNVC VLIDVGTKPG GRSIYHLWKC FDINSNVAGA 370 380 390 400 410 420 CGEIKAMLGP GGVHLLNPLV ASQNFEYKMS NILDKPLESV FGHISVLPGA FSAYRYEALQ 430 440 450 460 470 480 NDVNGHGPLE KYFLGEKMHG SDADIFTANM YLAEDRILCY ELVAKKGAAW VLHYCSNAYG 490 500 510 520 530 540 ETDVPDTVAE FISQRRRWLN GSFFAGIYSM VHWSKVWSSN HNILRCIMFM FEDLYQLYNL 550 560 570 580 590 600 IFSWFAIGNF YLVFYIMTTS INSVPNPPFA KNAGSVIHTV LNYLYVLLLI IQIIIAMGNR 610 620 630 640 650 660 PQGYKWAYFM IIVFFAILMG YVLFCTAWIT IEGVKTAVET AQHNQDAQFI ALIKQNSFRD 670 680 690 700 710 720 VIVSVCSTYI MYFVSSLLFL DPWHIFTSLI PYIFMSPSYI NVLNIYAFCN THDVSWGTKG 730 740 750 760 770 780 DNGVSTDLGV VKSTKGKDGV HEVEVDLPTE QKDLNEQYEE ACMALKVAPI PEVTKRDAKT 790 800 810 820 830 840 KQEDYYKSFR TTLVTCWIIS NLILVTIIIN GQEVWQWFGD YEKRATVYLS FILWSVAGLS 850 AIRFVGSILY LFMKIFTG

References

[1]	"Isolation and characterization of two chitin synthase genes of Rhizopus oligosporus." Motoyama T., Sudoh M., Horiuchi H., Ohta A., Takagi M. Biosci. Biotechnol. Biochem. 58:1685-1693(1994) [PubMed: 7765484] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	D10159 Genomic DNA. Translation: BAA01023.1.
PIR	JC2308.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR004834. Chitin_synth. IPR013616. Chitin_synth_N. [Graphical view]
Pfam	PF01644. Chitin_synth_1. 1 hit. PF08407. Chitin_synth_1N. 1 hit. [Graphical view]
ProDom	PD002998. Chitin_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

CHS1_RHIOL

Accession

Primary (citable) accession number: P30594

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	May 31, 2011
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program