Chitin synthase 2 - Neurospora crassa

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Reviewed, UniProtKB/Swiss-Prot P30589 (CHS2_NEUCR)

Last modified October 13, 2009. Version 64. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein names

Recommended name:
    Chitin synthase 2
    EC=2.4.1.16
Alternative name(s):
    Chitin-UDP acetyl-glucosaminyl transferase 2

Gene names

Name:	chs-2
ORF Names:	NCU05239

Organism

Neurospora crassa [Complete proteome]

Taxonomic identifier

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length	1371 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Plays a major role in cell wall biogenesis.
Catalytic activity	UDP-N-acetyl-D-glucosamine + (1,4-(N-acetyl-beta-D-glucosaminyl))(n) = UDP + (1,4-(N-acetyl-beta-D-glucosaminyl))(n+1).
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the chitin synthase family.
Sequence caution	The sequence CAA54816.1 differs from that shown. Reason: Frameshift at positions 321 and 452.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW chitin biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	chitin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1371

Chitin synthase 2

PRO_0000193702

Regions

Topological domain

1022

Extracellular Potential

Transmembrane

21

Potential

Topological domain

17

Cytoplasmic Potential

Transmembrane

21

Potential

Topological domain

14

Extracellular Potential

Transmembrane

21

Potential

Topological domain

23

Cytoplasmic Potential

Transmembrane

21

Potential

Topological domain

8

Extracellular Potential

Transmembrane

21

Potential

Topological domain

78

Cytoplasmic Potential

Transmembrane

21

Potential

Topological domain

11

Extracellular Potential

Transmembrane

21

Potential

Topological domain

51

Cytoplasmic Potential

Compositional bias

19

Poly-Gln

Amino acid modifications

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Glycosylation

1

N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict

1

K → D in CAA54816. Ref.2

Sequence conflict

2

PT → SP in CAA54816. Ref.2

Sequence conflict

3

KKK → EKN in CAA54816. Ref.2

Sequence conflict

1

A → V in CAA54816. Ref.2

Sequence conflict

2

SS → II in CAA54816. Ref.2

Sequence conflict

1

K → M in CAA54816. Ref.2

Sequence conflict

1

K → M in AAA33582. Ref.3

Sequence conflict

1

G → V in CAA54816. Ref.2

Sequence conflict

4

AVPV → CRSL in CAA54816. Ref.2

Sequence conflict

1

R → K in CAA54816. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

P30589-1 [UniParc].

Last modified January 15, 2008. Version 3.
Checksum: D8547DB5E4F121A8
Show »

1,371

153,962

        10         20         30         40         50         60 
MAGYGHSTAG GFGSGSGSGP PGPQYMLPQY DEGDDPDADA TPAGQGVRLL TNLDNSSYIS 

        70         80         90        100        110        120 
VSEITSQSSH RDNIRPYVMP LLSQPFHTNA STQTHTRTQS PPFHSNTSPK TKQGHDEAVV 

       130        140        150        160        170        180 
VNTPPAPFPS LSRSVPNKGP ENQEHQGKKT DLETPPNSNQ KEHAATTEED DSDMGKAVTF 

       190        200        210        220        230        240 
AGAPEILGSE DPQQQYAHLP HYPPPYSPFR PEEQDQFLMR QQQRQQQQQQ QQQQQQQQQA 

       250        260        270        280        290        300 
KAQPQQQGES QLREGGKLHK RKRLGQVKPL KLSAPPPTLA LPTIPEGTSP RRASLREARK 

       310        320        330        340        350        360 
KKQRSKQRLS NITEERDSTR SLTPPPSVHF GSPKRRNSEA ARSHIIPNHS SRLRQAYEPS 

       370        380        390        400        410        420 
IDARTYEPSL DTRTYEPSIS DRRHMYEPSI DERSSYMDPP RIPPPDGGSY VSSYMGTESM 

       430        440        450        460        470        480 
VSGHGRPWSP ESATGYRVPP QGRYEPSEID GHARPGTPGS SYGNARRPLP SAPAPLHYNS 

       490        500        510        520        530        540 
PSRAASHYPR YHGGYADDVT VSMGPDDDRT DIFGPETDLS ETRHLNDAYG FRSSQITLSE 

       550        560        570        580        590        600 
DPHGTHARSR YDDEDDVSTT YSSNTGTSAS GVDKFEHYGP IPEEGKHERR GVRPPQMSRK 

       610        620        630        640        650        660 
EVQLINGELV LECKIPTILY SFLPRRDEVE FTHMRYTAVT CDPDDFVARG YKLRQNIGRT 

       670        680        690        700        710        720 
ARETELFICV TMYNEDEFGF TRTMHAVMKN ISHFCSRNKS RTWGADGWQK IVVCVVSDGR 

       730        740        750        760        770        780 
EIIHPRTLDA LAAMGVYQHG IAKNFVNQKA VQAHVYEYTT QVSLDSDLKF KGAEKGIVPC 

       790        800        810        820        830        840 
QMIFCLKEKN QKKLNSHRWF FNAFGKALNP NVCILLDVGT RPGGTSLYHL WKAFDTDSNV 

       850        860        870        880        890        900 
AGACGEIKAM KGRFGGNLLN PLVASQNFEY KMSNILDKPL ESVFGYITVL PGALSAYRYH 

       910        920        930        940        950        960 
ALQNDETGHG PLSQYFKGET LHGQHADVFT ANMYLAEDRI LCWELVAKRG ERWVLKYVKG 

       970        980        990       1000       1010       1020 
CTGETDVPDT VPEFVSQRRR WLNGAFFAAV YSLVHFRQIW KTDHTFMRKA LLHVEFLYHL 

      1030       1040       1050       1060       1070       1080 
LQLLFTYFSL ANFYLAFYFI AGGLADPHVD PFNSDGHVAR IIFNILRYVC VLLICTQFIL 

      1090       1100       1110       1120       1130       1140 
SLGNRPQGAK RMYLASMIIY AVIMVYTTFA TIFIVVRQIQ PSQKSDDKPD LELGNNVFTN 

      1150       1160       1170       1180       1190       1200 
LIVSVASTLG LYFVMSFLYL DPWHMFTSAI QYFVLLPSYI CTLQIYAFCN THDVTWGTKG 

      1210       1220       1230       1240       1250       1260 
DNVMRTDLGG AIGKGSTVEL EMPSDQLDID SGYDECLRNL RDRVMVPAVP VSEDQLQQDY 

      1270       1280       1290       1300       1310       1320 
YKSVRTYMVV SWMVANATLA MAVSEAYGDS EIGDNFYLRF ILWAVAALAL FRALGSTTFA 

      1330       1340       1350       1360       1370 
AINLVSALVE GRVRLRLNMK GFRWIKEKWG DADVKGKFEG LGDRARGLAR R

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. Birren B.W. Nature 422:859-868(2003) [PubMed: 12712197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[2]	"The Neurospora crassa chs-2 gene encodes a non-essential chitin synthase." Din A.B., Yarden O. Microbiology 140:2189-2197(1994) [PubMed: 7952169] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 271-1371.
[3]	"Classification of fungal chitin synthases." Bowen A.R., Chen-Wu J.L.-P., Momany M., Young R., Szaniszlo P.J., Robbins P.W. Proc. Natl. Acad. Sci. U.S.A. 89:519-523(1992) [PubMed: 1731323] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 677-865.

Cross-references

Sequence databases
	AABX02000019 Genomic DNA. Translation: EAA32767.2. X77782 Genomic DNA. Translation: CAA54816.1. Frameshift. M82951 Genomic DNA. Translation: AAA33582.1.
PIR	B45189. T47246.
RefSeq	XP_962003.2.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Genome annotation databases
GeneID	3878151.
KEGG	ncr:NCU05239.
UCSC	F48A11.1. c. elegans.
Enzyme and pathway databases
BRENDA	2.4.1.16. 266.
Family and domain databases
InterPro	IPR004834. Chitin_synth. IPR013616. Chitin_synth_N. [Graphical view]
Pfam	PF01644. Chitin_synth_1. 1 hit. PF08407. Chitin_synth_1N. 1 hit. [Graphical view]
ProDom	PD002998. Chitin_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

CHS2_NEUCR

Accession

Primary (citable) accession number: P30589
Secondary accession number(s): Q7RVF4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	January 15, 2008
Last modified:	October 13, 2009
This is version 64 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents