P30588 (CHS3_NEUCR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chitin synthase 3
EC=2.4.1.16

Alternative name(s):
Chitin-UDP acetyl-glucosaminyl transferase 3

Gene names

Name:	chs-3
ORF Names:	G65A3.040, NCU04251

Organism

Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)

Taxonomic identifier

367110 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length	888 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays a major role in cell wall biogenesis.
Catalytic activity	UDP-N-acetyl-D-glucosamine + (1,4-(N-acetyl-beta-D-glucosaminyl))(n) = UDP + (1,4-(N-acetyl-beta-D-glucosaminyl))(n+1).
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the chitin synthase family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW chitin biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	chitin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 888	888	Chitin synthase 3		PRO_0000193703
Regions
	Transmembrane	532 – 551	20	Helical; Potential
	Transmembrane	572 – 594	23	Helical; Potential
	Transmembrane	607 – 629	23	Helical; Potential
	Transmembrane	642 – 664	23	Helical; Potential
	Transmembrane	684 – 706	23	Helical; Potential
	Transmembrane	814 – 836	23	Helical; Potential
	Transmembrane	860 – 882	23	Helical; Potential
Experimental info
	Sequence conflict	94	1	H → Q in AAF00101. Ref.1
	Sequence conflict	372	1	K → E in AAA33581. Ref.4
	Sequence conflict	388	1	E → D in AAA33581. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P30588 [UniParc]. Last modified August 31, 2004. Version 2. Checksum: CD6D3F2D8A50DC0B	FASTA	888	100,390
	10 20 30 40 50 60 MDPRMHTAPP AGHPIQPGYQ LEDNPYHHQQ GFDIPAGPGR YSPGDALHIQ TPQPIEGMGG 70 80 90 100 110 120 YNAPGQHYTP DYAVNPEEHH DAYYNQPYEP QVGHDPYAAA PTPPVAGYQA HDDQRPMLMH 130 140 150 160 170 180 TDSQVGQSDP YHDEPQPPTN NAPIKRWKTV KQVLLYRGNL VLDCPIPPKL LNQLPHGERD 190 200 210 220 230 240 EFTHMRYSAA TCDPSEFYEE NFTLRQKLFS KPRHTELFIV VTMYNEDEIL FARTMIGVFK 250 260 270 280 290 300 NIEYMCKRTE SKTWGKDAWK KIVVCVVSDG RAKINPRTRA LLAGMGVYQE GIAKQQVNGK 310 320 330 340 350 360 DVTAHIYEYT TQVGMTIKND VVQLIPKQQP VQMLFCLKEK NQKKINSHRW FFQAFGRVLD 370 380 390 400 410 420 PNICVLIDAG TKPGGSSIYH LWKAFDLEPM CAGACGEIKA MLGTGGKNLI NPLVATQNFE 430 440 450 460 470 480 YKMSNILDKP LESAFGFISV LPGAFSAYRY VALQNDKNGQ GPLEKYFAGE KLHGGDAGIF 490 500 510 520 530 540 TANMYLAEDR ILCFELVTKR NCHWILQYVK SATGETDVPA DLTELILQRR RWLNGSFFAA 550 560 570 580 590 600 IYAIVHFHQF FRSDHSFLRK IAFFIEFVFQ TVNMIFAWFA IGNFFLVFKI LTTGLGDEKL 610 620 630 640 650 660 LGTVGQILGV VFAWAYGVTL ITCFVLSMGN RPAGSPRLYM GMVVFWAIIF IYLMFAAIYI 670 680 690 700 710 720 AVVAIQTDVQ KGLSFTDLFR NELFYTLIVS VVSTYGIWLI ASLLMFDPWH MVTSMVQYML 730 740 750 760 770 780 LSPTYTNVLN VYAFCNTHDI SWGTKGDDKP DKLPSVNTKD GQGKTDLPDE GDLNASYERE 790 800 810 820 830 840 LQVFSRKYVK PVTAPTSAQL EEKQMDYYRG VRSMVVLVWM ITNFALCAVV LSTAGLERID 850 860 870 880 PEEGSQEQQT TKRATIYMSV VLWSVAVLSG FKFVGACWFL VVRMFRGV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Neurospora crassa chs-3 gene encodes an essential chitin synthase." Beth-Din A., Yarden O. Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[2]	"What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence." Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U. Nucleic Acids Res. 31:1944-1954(2003) [PubMed: 12655011] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[3]	"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed: 12712197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[4]	"Classification of fungal chitin synthases." Bowen A.R., Chen-Wu J.L.-P., Momany M., Young R., Szaniszlo P.J., Robbins P.W. Proc. Natl. Acad. Sci. U.S.A. 89:519-523(1992) [PubMed: 1731323] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 228-388.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF127086 Genomic DNA. Translation: AAF00101.1. AL513411 Genomic DNA. Translation: CAC28596.1. AABX02000013 Genomic DNA. Translation: EAA32006.1. M82950 Genomic DNA. Translation: AAA33581.1.
PIR	E38192.
RefSeq	XP_961242.1. XM_956149.1.
UniGene	Ncr.8273.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	EFNCRT00000003823; EFNCRP00000003823; EFNCRG00000003819.
GeneID	3877405.
KEGG	ncr:NCU04251.
NMPDR	fig|5141.1.peg.1524.
Phylogenomic databases
eggNOG	fuNOG04531.
GeneTree	EFGT00050000001909.
OMA	TMYNEDD.
OrthoDB	EOG4GMZ52.
Family and domain databases
InterPro	IPR004834. Chitin_synth. IPR004835. Chitin_synth_fng. IPR013616. Chitin_synth_N. [Graphical view]
KO	K00698.
Pfam	PF01644. Chitin_synth_1. 1 hit. PF08407. Chitin_synth_1N. 1 hit. PF03142. Chitin_synth_2. 1 hit. [Graphical view]
ProDom	PD002998. Chitin_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

CHS3_NEUCR

Accession

Primary (citable) accession number: P30588
Secondary accession number(s): Q1K7J0, Q9C209, Q9UVU7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	August 31, 2004
Last modified:	December 14, 2011
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program