Reviewed, UniProtKB/Swiss-Prot P30584 (CHSA_EMENI)

Names and origin

Protein names

Recommended name:
    Chitin synthase A
    EC=2.4.1.16
Alternative name(s):
    Chitin-UDP acetyl-glucosaminyl transferase A
    Class-II chitin synthase A

Gene names

Name:	chsA
Synonyms:	chs2
ORF Names:	AN7032

Organism

Emericella nidulans (Aspergillus nidulans) [Complete proteome]

Taxonomic identifier

162425 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › Emericella

Protein attributes

Sequence length	1013 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Plays a major role in cell wall biogenesis.
Catalytic activity	UDP-N-acetyl-D-glucosamine + (1,4-(N-acetyl-beta-D-glucosaminyl))(n) = UDP + (1,4-(N-acetyl-beta-D-glucosaminyl))(n+1).
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the chitin synthase family. Class II subfamily.
Sequence caution	The sequence EAA61678.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW chitin biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	chitin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1013	1013	Chitin synthase A		PRO_0000193691
Regions
	Transmembrane	508 – 528	21	Potential
	Transmembrane	646 – 666	21	Potential
	Transmembrane	686 – 706	21	Potential
	Transmembrane	721 – 741	21	Potential
	Transmembrane	759 – 779	21	Potential
	Transmembrane	892 – 912	21	Potential
	Transmembrane	919 – 939	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	P30584-1 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: DD5D2E9523B0778A	FASTA	1,013	113,679
	10 20 30 40 50 60 MDCQNGRRAN RTVRFARTAE SRYPERYSYE YDPEETLSRA APSMRNAPTI PPPTASGADE 70 80 90 100 110 120 MRYTASRPAS PARPWSPTRA ADWVRPPSAA ASYYERADIN GSPRPGTPSS RYGGSPRRPL 130 140 150 160 170 180 PPAPLFSKPG TTTQDTKIDI GDGEEDPFGG GGRTISSRHG PQGSVQSFTS ESTFIADETD 190 200 210 220 230 240 LEKVDLDEYE EESNETKSMV DPNLHYGPAP EKQSRRGVRN AQMAKKEVQL VNGELILECK 250 260 270 280 290 300 IPTILHSFLP RRDDREFTHM RYTAVTCDPD DFTQRGYKLR QQIGRTMRET ELFICITMYN 310 320 330 340 350 360 EDETHFTRTM HGVMQNISHF CSRSKSRTWG KDGWKKIVVC IISDGRKKVH PRTLNALAAL 370 380 390 400 410 420 GVYQEGIAKN VVNQKQVNAH VYEYTTQVSL DSDLKFKGAE KGIVPCQVIF CLKEHNQKKL 430 440 450 460 470 480 NSHRWFFNAF GRALQPNICI LLDVGTRPEP TALYHLWKAF DQDSNVAGAA GEIKASKGKN 490 500 510 520 530 540 MLGLLNPLVA SQNFEYKMSN ILDKPLESVF GYITVLPGAL SAYRFFALQN DAEGNGPLNQ 550 560 570 580 590 600 YFKGETLHGK DADVFTANMY LAEDRILCWE LVAKREERWV LRFVKSAVGE TDVPDSIPEF 610 620 630 640 650 660 ISQRRRWLNG AFFAAVYSIV NVKQLWKTDH SLARKILLQI ESVYQLLQLI FTYFGLANFY 670 680 690 700 710 720 LAFFFIAGSL TDEKIDPFGH NMGKYIFIVL RYACVLVMCL QFIFSMGNRP QGAKKLYLSS 730 740 750 760 770 780 MIVYSIVMAY TAFCTLYLIV LELMAKTGHD VPITMSDTLF VNIVVSLLST VGLYFFTSFM 790 800 810 820 830 840 YLDPWHMFTS SAQYFALLPS YICTLQCYAF CNTHDVTWGT KGDNTINTDL GTARIINGSI 850 860 870 880 890 900 VEVEMPSEQL DIDSGYDAAL RNLRDRLEVP DPGVSESQQQ EDYYRAVRTY MVSVWMVANV 910 920 930 940 950 960 VLAMAVSEVY GVGSSGTNVY LAIILWSVAV LAIIRAIGST AYAVLYLIQK LVEGKAKFQA 970 980 990 1000 1010 GNIASANASA AGSSLGTKSN VSYGSKGLNM TDRINETKWA ISRGMQKAMF WKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of two chitin synthase genes from Aspergillus nidulans." Yanai K., Kojima N., Takaya N., Horiuchi H., Ohta A., Takagi M. Biosci. Biotechnol. Biochem. 58:1828-1835(1994) [PubMed: 7765508] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: FGSC 89.
[2]	"Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae." Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S., Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V., Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S., Braus G.H. , Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H., Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K., Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R., Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C., Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L., Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W. Nature 438:1105-1115(2005) [PubMed: 16372000] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FGSC 4.
[3]	"Classification of fungal chitin synthases." Bowen A.R., Chen-Wu J.L.-P., Momany M., Young R., Szaniszlo P.J., Robbins P.W. Proc. Natl. Acad. Sci. U.S.A. 89:519-523(1992) [PubMed: 1731323] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 303-491.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D21268 Genomic DNA. Translation: BAA04806.1. AACD01000117 Genomic DNA. Translation: EAA61678.1. Sequence problems. M82939 Genomic DNA. Translation: AAA33303.1.
PIR	JC2314.
RefSeq	XP_664636.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Genome annotation databases
GeneID	2870113.
KEGG	ani:AN7032.2.
Family and domain databases
InterPro	IPR004834. Chitin_synth. IPR013616. Chitin_synth_N. [Graphical view]
Pfam	PF01644. Chitin_synth_1. 1 hit. PF08407. Chitin_synth_1N. 1 hit. [Graphical view]
ProDom	PD002998. Chitin_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

CHSA_EMENI

Accession

Primary (citable) accession number: P30584
Secondary accession number(s): Q5AXE8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	October 1, 1996
Last modified:	October 13, 2009
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)