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Protein

Enolase 1

Gene

ENO1

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.

Cofactori

Mg2+Note: Mg2+ is required for catalysis and for stabilizing the dimer.

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (TDH3)
  2. Phosphoglycerate kinase (PGK1)
  3. Phosphoglycerate mutase (GPM2), Phosphoglycerate mutase (GPM1)
  4. Enolase 1 (ENO1)
  5. Pyruvate kinase (CDC19)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei161SubstrateBy similarity1
Binding sitei170SubstrateBy similarity1
Active sitei213Proton donorBy similarity1
Metal bindingi248MagnesiumBy similarity1
Metal bindingi297MagnesiumBy similarity1
Binding sitei297SubstrateBy similarity1
Metal bindingi324MagnesiumBy similarity1
Binding sitei324SubstrateBy similarity1
Active sitei349Proton acceptorBy similarity1
Binding sitei400SubstrateBy similarity1

GO - Molecular functioni

  • high molecular weight kininogen binding Source: CGD
  • magnesium ion binding Source: InterPro
  • phosphopyruvate hydratase activity Source: CGD
  • protein-glutamine gamma-glutamyltransferase activity Source: CGD

GO - Biological processi

  • entry into host Source: CGD
  • fibrinolysis Source: CAFA
  • filamentous growth Source: CGD
  • filamentous growth of a population of unicellular organisms in response to biotic stimulus Source: CGD
  • fungal-type cell wall organization or biogenesis Source: CGD
  • gluconeogenesis Source: CGD
  • glycolytic process Source: CGD
  • induction by symbiont of host defense response Source: CGD

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187

Protein family/group databases

MoonProtiP30575

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase 1 (EC:4.2.1.11)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase
2-phosphoglycerate dehydratase
Gene namesi
Name:ENO1
Ordered Locus Names:CAALFM_C108500CA
ORF Names:CaO19.395, CaO19.8025
OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Taxonomic identifieri237561 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeCandida/Lodderomyces cladeCandida
Proteomesi
  • UP000000559 Componenti: Chromosome 1

Organism-specific databases

CGDiCAL0000185645 ENO1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Protein family/group databases

Allergomei785 Cand a Enolase

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001340431 – 440Enolase 1Add BLAST440

Proteomic databases

PRIDEiP30575

2D gel databases

COMPLUYEAST-2DPAGEP30575

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

  • high molecular weight kininogen binding Source: CGD

Protein-protein interaction databases

BioGridi1229577, 1 interactor

Structurei

3D structure databases

ProteinModelPortaliP30575
SMRiP30575
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni376 – 379Substrate bindingBy similarity4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi118 – 126Poly-Ala9

Sequence similaritiesi

Belongs to the enolase family.Curated

Phylogenomic databases

InParanoidiP30575
KOiK01689
OMAiEFMIIPV
OrthoDBiEOG092C2W5X

Family and domain databases

CDDicd03313 enolase, 1 hit
Gene3Di3.20.20.120, 1 hit
3.30.390.10, 1 hit
HAMAPiMF_00318 Enolase, 1 hit
InterProiView protein in InterPro
IPR000941 Enolase
IPR036849 Enolase-like_C_sf
IPR029017 Enolase-like_N
IPR034390 Enolase-like_superfamily
IPR020810 Enolase_C
IPR020809 Enolase_CS
IPR020811 Enolase_N
PANTHERiPTHR11902 PTHR11902, 1 hit
PfamiView protein in Pfam
PF00113 Enolase_C, 1 hit
PF03952 Enolase_N, 1 hit
PIRSFiPIRSF001400 Enolase, 1 hit
PRINTSiPR00148 ENOLASE
SFLDiSFLDG00178 enolase, 1 hit
SFLDS00001 Enolase, 1 hit
SMARTiView protein in SMART
SM01192 Enolase_C, 1 hit
SM01193 Enolase_N, 1 hit
SUPFAMiSSF51604 SSF51604, 1 hit
TIGRFAMsiTIGR01060 eno, 1 hit
PROSITEiView protein in PROSITE
PS00164 ENOLASE, 1 hit

Sequencei

Sequence statusi: Complete.

P30575-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYATKIHAR YVYDSRGNPT VEVDFTTDKG LFRSIVPSGA STGVHEALEL
60 70 80 90 100
RDGDKSKWLG KGVLKAVANV NDIIAPALIK AKIDVVDQAK IDEFLLSLDG
110 120 130 140 150
TPNKSKLGAN AILGVSLAAA NAAAAAQGIP LYKHIANISN AKKGKFVLPV
160 170 180 190 200
PFQNVLNGGS HAGGALAFQE FMIAPTGVST FSEALRIGSE VYHNLKSLTK
210 220 230 240 250
KKYGQSAGNV GDEGGVAPDI KTPKEALDLI MDAIDKAGYK GKVGIAMDVA
260 270 280 290 300
SSEFYKDGKY DLDFKNPESD PSKWLSGPQL ADLYEQLISE YPIVSIEDPF
310 320 330 340 350
AEDDWDAWVH FFERVGDKIQ IVGDDLTVTN PTRIKTAIEK KAANALLLKV
360 370 380 390 400
NQIGTLTESI QAANDSYAAG WGVMVSHRSG ETEDTFIADL SVGLRSGQIK
410 420 430 440
TGAPARSERL AKLNQILRIE EELGSEAIYA GKDFQKASQL
Length:440
Mass (Da):47,232
Last modified:April 1, 1993 - v1
Checksum:i6621AFD66F275C49
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L04943 Genomic DNA Translation: AAB46358.1
M93712 mRNA Translation: AAA34341.1
L10290 Genomic DNA Translation: AAA71939.1
CP017623 Genomic DNA Translation: AOW26488.1
PIRiA40624
RefSeqiXP_711883.1, XM_706790.2

Genome annotation databases

EnsemblFungiiAOW26488; AOW26488; CAALFM_C108500CA
GeneIDi3646493
KEGGical:CAALFM_C108500CA

Similar proteinsi

Entry informationi

Entry nameiENO1_CANAL
AccessioniPrimary (citable) accession number: P30575
Secondary accession number(s): A0A1D8PEA6, Q59QC3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: May 23, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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