P30573 (CHS3_CANAX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chitin synthase 3
EC=2.4.1.16

Alternative name(s):
Chitin-UDP acetyl-glucosaminyl transferase 3
Class-IV chitin synthase 3

Gene names

Name:

CHS3

Organism

Candida albicans (Yeast)

Taxonomic identifier

5476 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	1213 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Formation and repair of the disk-shaped septum in yeast and the cross walls of the hyphal phase.
Catalytic activity	UDP-N-acetyl-D-glucosamine + (1,4-(N-acetyl-beta-D-glucosaminyl))(n) = UDP + (1,4-(N-acetyl-beta-D-glucosaminyl))(n+1).
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the chitin synthase family. Class IV subfamily.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological process	cell wall chitin biosynthetic process Inferred from mutant phenotype. Source: CGD cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW cellular response to mechanical stimulus Inferred from mutant phenotype. Source: CGD hyphal growth Inferred from mutant phenotype. Source: CGD pathogenesis Inferred from mutant phenotype. Source: CGD
Cellular component	cell septum Inferred from direct assay. Source: CGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	chitin synthase activity Inferred from mutant phenotype. Source: CGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1213	1213	Chitin synthase 3		PRO_0000193688
Regions
	Transmembrane	169 – 189	21	Helical; Potential
	Transmembrane	201 – 221	21	Helical; Potential
	Transmembrane	451 – 471	21	Helical; Potential
	Transmembrane	1017 – 1037	21	Helical; Potential
	Transmembrane	1040 – 1060	21	Helical; Potential
	Transmembrane	1066 – 1086	21	Helical; Potential
	Transmembrane	1091 – 1111	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P30573 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: E6D313EEACD10BC6	FASTA	1,213	136,208
	10 20 30 40 50 60 MSNFRDSSSP RRGYSEFDPE SGEGLGRKKS LIRPERSRMD ESHPRFHYTQ VANQESNHIK 70 80 90 100 110 120 VQPSSTGVDP RKSNELSTSR SHLSNYATPP HQEEEEDEGI PLMDIHNASP NVSSDQNNDL 130 140 150 160 170 180 KGGREVYGLN DEINDYGSSP KKNQVISSSR PMNNEKPAKP KHDIYFWKVY CYAITFWAPA 190 200 210 220 230 240 PLLKLFGLPT KDRQFAWREK IGLISCILYV GAFVAYLTFG FTKTVCSSQV VRTQINHVNG 250 260 270 280 290 300 GYLIINGRAY DLTSSQHPKA AGIQAGSNVL YPPMNAGGKD ASFLFQNVNG NCKGLIKPRD 310 320 330 340 350 360 NCSIPYDGDE LAWYMPCRLF NQDGSTKPNN TFAYYKGWAC HTSETARDAY YKLKVNGDVY 370 380 390 400 410 420 FTWDDVKNSS RNLVVYSGNV LDLDLINWIE TDDVTYPELF DKLRDDETYR GLDISLVLTN 430 440 450 460 470 480 SEERQAARCL TEIIKVGSID TDTIGCIASK VVLYMSLVFI LSVVVVKFIM ACWFKWVTSR 490 500 510 520 530 540 KQGATMYDSK AWAKRNREIE DWVDHDHGIG AEVKTVPVKA RANYKAAKTN RQSVFHRAQK 550 560 570 580 590 600 LSLGPNADLS QYYDNPNALS KTFKYTTMST QAALLGRNGY GKRGNNANKS VSGGFNGRQS 610 620 630 640 650 660 NLYLTDQGSS TDLLNRPVSS YNPFDSMGDD SIVINGLSPD IIHPDVVPQP PVEYQPFGYP 670 680 690 700 710 720 LAHTINLVTC YSEDEEGIRI TLDSIATTDY PNSHKLILVI CDGIIKGSGN DETTPDIVLD 730 740 750 760 770 780 MMSDLTVPRD EVEAYSYVAV AQGSKRHNMA KVYAGFYKYN DETVPPEKQQ RIPMITIVKC 790 800 810 820 830 840 GTPEEASAPK PGNRGKRDSQ IILMSFLQKV VFDERMTSLE YEMLQSIWRI TGLMAEFYEI 850 860 870 880 890 900 VLMVDADTKV FPDSLTHMVA EMVKDPTIMG LCGETKISNK AQTWVTAIQV FEYYISHHQA 910 920 930 940 950 960 KAFESIFGGV TCLPGCFCMY RIKAPKGSDG YWVPILANPD IVERYSDNVV DTLHRKNLLL 970 980 990 1000 1010 1020 LGEDRYLSSL MLRTFPTRKQ VFVPKAACKT VVPDKFKVLL SQRRRWINST VHNLFELVLV 1030 1040 1050 1060 1070 1080 KDLCGTFCFS MQFVIFIELI GTLVLPAAIT FTIYVIIVAI VSKPTPVMSL VLLAVIFGLP 1090 1100 1110 1120 1130 1140 GCLIVITVSS LSYLVYFVIY LFALPIWNFV LPSYAYWKFD DFSWGETRTV AGGDKGDHSA 1150 1160 1170 1180 1190 1200 VEGKFDSSKI AMKRWREWER ERRSTENRKQ QQQQQLTNNS SNNLAVPGAA WDPSNTGGNL 1210 IDDLSQGSSS GSS

References

[1]	"Cloning of the chitin synthase 3 gene from Candida albicans and its expression during yeast-hyphal transition." Sudoh M., Nagahashi S., Arisawa M., Takagi M. Mol. Gen. Genet. 241:351-358(1993) [PubMed: 8246889] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	D13454 Genomic DNA. Translation: BAA02707.1.
PIR	S39951.
3D structure databases
ProteinModelPortal	P30573.
ModBase	Search...
Protein-protein interaction databases
STRING	P30573.
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
CGD	CAL0006283. CHS3.
Phylogenomic databases
PhylomeDB	P30573.
Family and domain databases
InterPro	IPR004835. Chitin_synth_fng. [Graphical view]
Pfam	PF03142. Chitin_synth_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHS3_CANAX

Accession

Primary (citable) accession number: P30573

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	January 25, 2012
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program