P30572 (CHS2_CANAX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chitin synthase 2
EC=2.4.1.16

Alternative name(s):
Chitin-UDP acetyl-glucosaminyl transferase 2

Gene names

Name:

CHS2

Organism

Candida albicans (Yeast)

Taxonomic identifier

5476 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	1009 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Formation and repair of the disk-shaped septum in yeast and the cross walls of the hyphal phase.
Catalytic activity	UDP-N-acetyl-D-glucosamine + (1,4-(N-acetyl-beta-D-glucosaminyl))(n) = UDP + (1,4-(N-acetyl-beta-D-glucosaminyl))(n+1).
Subcellular location	Cell membrane; Multi-pass membrane protein.
Developmental stage	Very high levels of CHS2 in cells undergoing hyphal outgrowth.
Sequence similarities	Belongs to the chitin synthase family.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW chitin biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	chitin synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1009	1009	Chitin synthase 2		PRO_0000193687
Regions
	Transmembrane	647 – 667	21	Helical; Potential
	Transmembrane	682 – 702	21	Helical; Potential
	Transmembrane	722 – 742	21	Helical; Potential
	Transmembrane	757 – 777	21	Helical; Potential
	Transmembrane	804 – 823	20	Helical; Potential
	Transmembrane	930 – 950	21	Helical; Potential
	Transmembrane	967 – 987	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P30572 [UniParc]. Last modified November 1, 1995. Version 2. Checksum: 182B660678549EF9	FASTA	1,009	115,586
	10 20 30 40 50 60 MSYNNPNNSN SHLRPHAYNN SRRDDSDGDE SSIEFLNQRS NTPLTQGTYN YHNTSTNSLN 70 80 90 100 110 120 FQQPEPIYRN QTRTSLSDSY YDHPIFDTSQ TQIQPPHDNP FTESYEMTDT SYQGNDHHYR 130 140 150 160 170 180 TGQPNHLMNP TYNQAFIPHV YDEEDNDEQE YDQRIQYNQF QGDHFDLAAI SYADDESQSQ 190 200 210 220 230 240 LDYVPTERVI PEGEEEEEEG ETSFEKEPGS ETISGPFGEE RSFEEPPPQQ EVRSKKLTRA 250 260 270 280 290 300 TGLNGHLVLD CPVADELLSK FPDYNPAEKS GGLSREFAFM RYTAVTCGPS NFYRDAYILR 310 320 330 340 350 360 PVHYPIPRQT ELMIVITMYN EDDILLGRTL KGVFKNIKYL ESKARSSTWG KDSWKKIVVC 370 380 390 400 410 420 IVSDGRTKIN ERAQALLAGL GVYQEGLAKS RVDDKKVQAH MFEYTTRVGI SKVTDDVVKL 430 440 450 460 470 480 TTEKVVPVQM LFCLKETNAK KINSHRWCFQ AIGQVLDPKI VVLLDCGTQP SGRSLYELWK 490 500 510 520 530 540 EFDRDHRVAG ACGEITTSLK KRQMITNPLV YGQNFEYKIS NILDKPTESS FGFISVLPGA 550 560 570 580 590 600 FSAYRFIALQ NDINGIGPLE KYFKGEFLHS SGELDPNDDE FQMKHLMLKE EAGIFTSNMY 610 620 630 640 650 660 LAEDRILCFE LVAKRGCNWL LRYCKSARAE TDVPEGLAEF ILQRRRWLNG SFFAAIYSLV 670 680 690 700 710 720 HFYKVWTSSH SFGRKIFLHI EFFYQLINLI VSWFSIGSYF LVFRILTTSL GDKALGFAPG 730 740 750 760 770 780 KILSVIFLWL YLASIVTTFV LSFGNKPKGT EKFYVTIVIF FAILMAYMIF AAIFMAVHSI 790 800 810 820 830 840 QDIYRSGTRI TVSLFFQNSE FRDLVVATSS TYALYFLASF LYFEPWHMFT SFVQYILLSP 850 860 870 880 890 900 SYVNVLNIYA FCNIDDISWG TKGEVGGKSL GEAKLREDGT FDVSVPISKE QINQSYLDQL 910 920 930 940 950 960 EKIRDPAPPE EKVLVTNTED YYAFIRSMTV LVWMFTNFVV IALVLETGGF NQFVEATDLA 970 980 990 1000 NLKSNRAAVF LTVILWTVAF MALFRFIGCI YYLITRLGRE IKASEHATK

References

[1]	"Expression of chitin synthase genes during yeast and hyphal growth phases of Candida albicans." Chen-Wu J.L.-P., Zwicker J., Bowen A.R., Robbins P.W. Mol. Microbiol. 6:497-502(1992) [PubMed: 1560778] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M82937 Genomic DNA. Translation: AAB59308.2.
PIR	S20538.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P30572.
Protein family/group databases
CAZy	GT2. Glycosyltransferase Family 2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR004834. Chitin_synth. IPR004835. Chitin_synth_fng. IPR013616. Chitin_synth_N. [Graphical view]
Pfam	PF01644. Chitin_synth_1. 1 hit. PF08407. Chitin_synth_1N. 1 hit. PF03142. Chitin_synth_2. 1 hit. [Graphical view]
ProDom	PD002998. Chitin_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

CHS2_CANAX

Accession

Primary (citable) accession number: P30572

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	November 1, 1995
Last modified:	October 19, 2011
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program