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P30569

- EC1_WHEAT

UniProt

P30569 - EC1_WHEAT

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Protein

EC protein I/II

Gene
N/A
Organism
Triticum aestivum (Wheat)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Binds 5 molecules of zinc. May have a role in Zn2+ homeostasis during embryogenesis.

GO - Molecular functioni

  1. zinc ion binding Source: InterPro
Complete GO annotation...

Keywords - Ligandi

Metal-binding, Metal-thiolate cluster, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
EC protein I/II
Alternative name(s):
Zinc metallothionein class II
OrganismiTriticum aestivum (Wheat)
Taxonomic identifieri4565 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeTriticum
ProteomesiUP000019116: Unplaced

Organism-specific databases

GrameneiP30569.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 8180EC protein I/IIPRO_0000197426Add
BLAST

Expressioni

Developmental stagei

Expressed during embryogenesis.

Inductioni

By abscisic acid (ABA).

Structurei

Secondary structure

1
81
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 73
Beta strandi14 – 163
Helixi20 – 223
Turni37 – 393
Beta strandi58 – 614
Helixi74 – 774

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KAKNMR-A31-81[»]
2L61NMR-A2-25[»]
2L62NMR-A2-25[»]
2MFPNMR-A2-27[»]

Miscellaneous databases

EvolutionaryTraceiP30569.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiIPR000316. Metallthion_15.
[Graphical view]
PfamiPF02068. Metallothio_PEC. 1 hit.
[Graphical view]
PRINTSiPR00877. MTPLANTPEC.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30569-1 [UniParc]FASTAAdd to Basket

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MGCDDKCGCA VPCPGGTGCR CTSARSGAAA GEHTTCGCGE HCGCNPCACG   50
REGTPSGRAN RRANCSCGAA CNCASCGSAT A 81
Length:81
Mass (Da):7,713
Last modified:January 23, 2007 - v2
Checksum:iA08668901106B7A7
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti51 – 511R → G AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X68289 mRNA. Translation: CAA48349.1.
X68290 mRNA. Translation: CAA48350.1.
PIRiS27369.
UniGeneiTa.2799.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X68289 mRNA. Translation: CAA48349.1 .
X68290 mRNA. Translation: CAA48350.1 .
PIRi S27369.
UniGenei Ta.2799.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2KAK NMR - A 31-81 [» ]
2L61 NMR - A 2-25 [» ]
2L62 NMR - A 2-25 [» ]
2MFP NMR - A 2-27 [» ]
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

Gramenei P30569.

Miscellaneous databases

EvolutionaryTracei P30569.

Family and domain databases

InterProi IPR000316. Metallthion_15.
[Graphical view ]
Pfami PF02068. Metallothio_PEC. 1 hit.
[Graphical view ]
PRINTSi PR00877. MTPLANTPEC.
ProtoNeti Search...

Publicationsi

  1. "Wheat Ec metallothionein genes. Like mammalian Zn2+ metallothionein genes, wheat Zn2+ metallothionein genes are conspicuously expressed during embryogenesis."
    Kawashima I., Kennedy T.D., Chino M., Lane B.G.
    Eur. J. Biochem. 209:971-976(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Neepawa.
    Tissue: Embryo.
  2. "Partial amino acid sequence of the wheat germ Ec protein. Comparison with another protein very rich in half-cystine and glycine. Wheat germ agglutinin."
    Hofmann T., Kells D.I.C., Lane B.G.
    Can. J. Biochem. Cell Biol. 62:908-913(1984)
    Cited for: PROTEIN SEQUENCE OF 2-60.
    Strain: cv. Thatcher.
    Tissue: Embryo.

Entry informationi

Entry nameiEC1_WHEAT
AccessioniPrimary (citable) accession number: P30569
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: January 23, 2007
Last modified: June 11, 2014
This is version 59 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing

Documents

  1. Metallothioneins
    Classification of metallothioneins and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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