P30569 (EC1_WHEAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: EC protein I/II Alternative name(s): Zinc metallothionein class II
Organism	Triticum aestivum (Wheat)
Taxonomic identifier	4565 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Triticum

Protein attributes

Sequence length	81 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds 5 molecules of zinc. May have a role in Zn2+ homeostasis during embryogenesis.
Developmental stage	Expressed during embryogenesis.
Induction	By abscisic acid (ABA).
Sequence similarities	Belongs to the metallothionein superfamily. Type 15 family.

Ontologies

Keywords
Ligand	Metal-binding Metal-thiolate cluster Zinc
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Molecular_function	zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.2

Chain

2 – 81

80

EC protein I/II

PRO_0000197426

Experimental info

Sequence conflict

51

1

R → G AA sequence Ref.2

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

81

Helix Strand Turn

Details...

Helix

5 – 7

3

Beta strand

14 – 16

3

Helix

20 – 22

3

Turn

37 – 39

3

Beta strand

58 – 61

4

Helix

74 – 77

4

Sequences

	Sequence		Length	Mass (Da)
	P30569 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: A08668901106B7A7	FASTA	81	7,713
	10 20 30 40 50 60 MGCDDKCGCA VPCPGGTGCR CTSARSGAAA GEHTTCGCGE HCGCNPCACG REGTPSGRAN 70 80 RRANCSCGAA CNCASCGSAT A

References

[1]	"Wheat Ec metallothionein genes. Like mammalian Zn2+ metallothionein genes, wheat Zn2+ metallothionein genes are conspicuously expressed during embryogenesis." Kawashima I., Kennedy T.D., Chino M., Lane B.G. Eur. J. Biochem. 209:971-976(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Neepawa. Tissue: Embryo.
[2]	"Partial amino acid sequence of the wheat germ Ec protein. Comparison with another protein very rich in half-cystine and glycine. Wheat germ agglutinin." Hofmann T., Kells D.I.C., Lane B.G. Can. J. Biochem. Cell Biol. 62:908-913(1984) Cited for: PROTEIN SEQUENCE OF 2-60. Strain: cv. Thatcher. Tissue: Embryo.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X68289 mRNA. Translation: CAA48349.1.
X68290 mRNA. Translation: CAA48350.1.

PIR

S27369.

UniGene

Ta.2799.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2KAK	NMR	-	A	31-81	[»]
2L61	NMR	-	A	2-25	[»]
2L62	NMR	-	A	2-25	[»]

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Organism-specific databases

Gramene

P30569.

Family and domain databases

InterPro

IPR000316. Metallthion_15.
[Graphical view]

Pfam

PF02068. Metallothio_PEC. 1 hit.
[Graphical view]

PRINTS

PR00877. MTPLANTPEC.

ProtoNet

Search...

Other

EvolutionaryTrace

P30569.

Entry information

Entry name

EC1_WHEAT

Accession

Primary (citable) accession number: P30569

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 55 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program