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P30569

- EC1_WHEAT

UniProt

P30569 - EC1_WHEAT

Protein

EC protein I/II

Gene
N/A
Organism
Triticum aestivum (Wheat)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Binds 5 molecules of zinc. May have a role in Zn2+ homeostasis during embryogenesis.

    GO - Molecular functioni

    1. zinc ion binding Source: InterPro

    Keywords - Ligandi

    Metal-binding, Metal-thiolate cluster, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    EC protein I/II
    Alternative name(s):
    Zinc metallothionein class II
    OrganismiTriticum aestivum (Wheat)
    Taxonomic identifieri4565 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeTriticum
    ProteomesiUP000019116: Unplaced

    Organism-specific databases

    GrameneiP30569.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 8180EC protein I/IIPRO_0000197426Add
    BLAST

    Expressioni

    Developmental stagei

    Expressed during embryogenesis.

    Inductioni

    By abscisic acid (ABA).

    Structurei

    Secondary structure

    1
    81
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi5 – 73
    Beta strandi14 – 163
    Helixi20 – 223
    Turni37 – 393
    Beta strandi58 – 614
    Helixi74 – 774

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2KAKNMR-A31-81[»]
    2L61NMR-A2-25[»]
    2L62NMR-A2-25[»]
    2MFPNMR-A2-27[»]
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP30569.

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    InterProiIPR000316. Metallthion_15.
    [Graphical view]
    PfamiPF02068. Metallothio_PEC. 1 hit.
    [Graphical view]
    PRINTSiPR00877. MTPLANTPEC.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P30569-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGCDDKCGCA VPCPGGTGCR CTSARSGAAA GEHTTCGCGE HCGCNPCACG   50
    REGTPSGRAN RRANCSCGAA CNCASCGSAT A 81
    Length:81
    Mass (Da):7,713
    Last modified:January 23, 2007 - v2
    Checksum:iA08668901106B7A7
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti51 – 511R → G AA sequence 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X68289 mRNA. Translation: CAA48349.1.
    X68290 mRNA. Translation: CAA48350.1.
    PIRiS27369.
    UniGeneiTa.2799.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X68289 mRNA. Translation: CAA48349.1 .
    X68290 mRNA. Translation: CAA48350.1 .
    PIRi S27369.
    UniGenei Ta.2799.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2KAK NMR - A 31-81 [» ]
    2L61 NMR - A 2-25 [» ]
    2L62 NMR - A 2-25 [» ]
    2MFP NMR - A 2-27 [» ]
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    Gramenei P30569.

    Miscellaneous databases

    EvolutionaryTracei P30569.

    Family and domain databases

    InterProi IPR000316. Metallthion_15.
    [Graphical view ]
    Pfami PF02068. Metallothio_PEC. 1 hit.
    [Graphical view ]
    PRINTSi PR00877. MTPLANTPEC.
    ProtoNeti Search...

    Publicationsi

    1. "Wheat Ec metallothionein genes. Like mammalian Zn2+ metallothionein genes, wheat Zn2+ metallothionein genes are conspicuously expressed during embryogenesis."
      Kawashima I., Kennedy T.D., Chino M., Lane B.G.
      Eur. J. Biochem. 209:971-976(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Neepawa.
      Tissue: Embryo.
    2. "Partial amino acid sequence of the wheat germ Ec protein. Comparison with another protein very rich in half-cystine and glycine. Wheat germ agglutinin."
      Hofmann T., Kells D.I.C., Lane B.G.
      Can. J. Biochem. Cell Biol. 62:908-913(1984)
      Cited for: PROTEIN SEQUENCE OF 2-60.
      Strain: cv. Thatcher.
      Tissue: Embryo.

    Entry informationi

    Entry nameiEC1_WHEAT
    AccessioniPrimary (citable) accession number: P30569
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 60 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Metallothioneins
      Classification of metallothioneins and list of entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3