Reviewed, UniProtKB/Swiss-Prot P30568 (GSTA_PLEPL)

Names and origin

Protein names	Recommended name:     Glutathione S-transferase A       Short name=GST-A     EC=2.5.1.18 Alternative name(s):     GST class-theta
Organism	Pleuronectes platessa (Plaice)
Taxonomic identifier	8262 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Euteleostei › Neoteleostei › Acanthomorpha › Acanthopterygii › Percomorpha › Pleuronectiformes › Pleuronectoidei › Pleuronectidae › Pleuronectinae › Pleuronectes

Protein attributes

Sequence length	225 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm.
Tissue specificity	Found in all the tissues examined. Highest values found in liver and in intestinal mucosa.
Induction	By epoxides and by antioxidants.
Sequence similarities	Belongs to the GST superfamily. Theta family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Transferase
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 225	225	Glutathione S-transferase A		PRO_0000185946
Regions
	Domain	3 – 85	83	GST N-terminal
	Domain	92 – 217	126	GST C-terminal
Sites
	Active site	13	1	By similarity
	Binding site	18	1	Glutathione Potential
Experimental info
	Sequence conflict	140	1	L → V figure 2 Ref.1
	Sequence conflict	143 – 144	2	EL → DV figure 2 Ref.1
	Sequence conflict	147 – 148	2	WE → SQ figure 2 Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P30568-1 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 37899602D07C077F	FASTA	225	25,724
	10 20 30 40 50 60 MAKDMTLLWG SGSPPCWRVM IVLEEKNLQA YNSKLLSFEK GEHKSAEVMS MNPRGQLPSF 70 80 90 100 110 120 KHGSKVLNES YAACMYLESQ FKSQGNKLIP DCPAEQAMMY QRMFEGLTLA QKMADVIYYS 130 140 150 160 170 180 WKVPEAERHD SAVKRNKENL STELKLWEEY LQKTSGSFVA GKSFSLADVS VFPGVAYLFR 190 200 210 220 FGLTEERYPQ LTAYYNSLKE RPSIKASWPP TWLESPQGQD MLKDV

References

[1]

"Cloning and characterization of the major hepatic glutathione S-transferase from a marine teleost flatfish, the plaice (Pleuronectes platessa), with structural similarities to plant, insect and mammalian Theta class isoenzymes." Leaver M.J., Scott K., George S.G. Biochem. J. 292:189-195(1993) [PubMed: 8503846] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X63761 mRNA. Translation: CAA45293.1.
PIR	S33308.
3D structure databases
HSSP	HSSP built from PDB template 1E6B based on UniProtKB Q9ZVQ3.
ModBase	Search...
Phylogenomic databases
HOVERGEN	P30568.
Enzyme and pathway databases
BRENDA	2.5.1.18. 74156.
Family and domain databases
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:1.20.1050.10. GST_C_like. 1 hit. G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSTA_PLEPL

Accession

Primary (citable) accession number: P30568

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	June 16, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)