P30560 (V1AR_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vasopressin V1a receptor
Short name=V1aR

Alternative name(s):
AVPR V1a
Antidiuretic hormone receptor 1a
Vascular/hepatic-type arginine vasopressin receptor

Gene names

Name:

Avpr1a

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	424 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which activate a phosphatidyl-inositol-calcium second messenger system. Involved in social memory formation. Ref.11
Subcellular location	Cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein. Note: Located in cytoplasmic vesicles dispersed throughout the cell cytoplasm and to the plasma membrane. Ref.8 Ref.10
Tissue specificity	Localized within gonadotropes of the anterior pituitary of the brain. Broadly distributed throughout the cerebral cortex. Ref.7 Ref.10
Post-translational modification	Palmitoylated on three cysteine residues, of which only two are identified. Ref.9
Miscellaneous	Overexpression of AVPR1A in the brain increases the duration of social memory.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family. Vasopressin/oxytocin receptor subfamily.
Sequence caution	The sequence CAA77748.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasmic vesicle Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	calcium-mediated signaling Inferred from mutant phenotype PubMed 12088846. Source: RGD cellular response to water deprivation Inferred from expression pattern PubMed 11796498. Source: RGD elevation of cytosolic calcium ion concentration Inferred from mutant phenotype PubMed 12088846. Source: RGD grooming behavior Inferred from mutant phenotype PubMed 18640147. Source: RGD maternal aggressive behavior Inferred from mutant phenotype PubMed 18640147. Source: RGD maternal behavior Inferred from mutant phenotype PubMed 18640147. Source: RGD myotube differentiation Inferred from expression pattern PubMed 18021262. Source: RGD negative regulation of female receptivity Inferred from mutant phenotype PubMed 16488546. Source: RGD negative regulation of transmission of nerve impulse Inferred from mutant phenotype PubMed 16304841. Source: RGD penile erection Inferred from mutant phenotype PubMed 18552879. Source: RGD positive regulation of cell growth Inferred from mutant phenotype PubMed 17395547. Source: RGD positive regulation of cell proliferation Inferred from mutant phenotype PubMed 17395547. Source: RGD positive regulation of cellular pH reduction Inferred from mutant phenotype PubMed 17762181. Source: RGD positive regulation of glutamate secretion Inferred from mutant phenotype PubMed 17877638. Source: RGD positive regulation of glycogen catabolic process Non-traceable author statement Ref.1. Source: UniProtKB positive regulation of heart rate Inferred from mutant phenotype PubMed 18339407. Source: RGD positive regulation of prostaglandin biosynthetic process Inferred from mutant phenotype PubMed 17254553. Source: RGD positive regulation of renal sodium excretion Inferred from mutant phenotype PubMed 18596120. Source: RGD positive regulation of systemic arterial blood pressure Inferred from mutant phenotype PubMed 17350115PubMed 18957383. Source: RGD positive regulation of urine volume Non-traceable author statement Ref.1. Source: UniProtKB positive regulation of vasoconstriction Inferred from mutant phenotype PubMed 17428891. Source: RGD regulation of corticotropin secretion Non-traceable author statement Ref.1. Source: UniProtKB regulation of systemic arterial blood pressure by vasopressin Traceable author statement PubMed 12369733. Source: RGD response to corticosterone stimulus Inferred from expression pattern PubMed 15475662. Source: RGD social behavior Inferred from direct assay Ref.11. Source: UniProtKB sperm ejaculation Inferred from mutant phenotype PubMed 18552879. Source: RGD telencephalon development Inferred from expression pattern PubMed 11021977. Source: RGD
Cellular_component	cytoplasmic vesicle Inferred from direct assay PubMed 11796498. Source: RGD cytoplasmic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Non-traceable author statement Ref.1. Source: UniProtKB plasma membrane Inferred from direct assay PubMed 16049168. Source: RGD
Molecular_function	V1A vasopressin receptor binding Inferred from direct assay Ref.1. Source: UniProtKB peptide hormone binding Inferred from mutant phenotype PubMed 12942143. Source: RGD vasopressin receptor activity Inferred from direct assay PubMed 19560475. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 424	424	Vasopressin V1a receptor		PRO_0000070201
Regions
	Topological domain	1 – 52	52	Extracellular Potential
	Transmembrane	53 – 76	24	Helical; Name=1; Potential
	Topological domain	77 – 88	12	Cytoplasmic Potential
	Transmembrane	89 – 110	22	Helical; Name=2; Potential
	Topological domain	111 – 125	15	Extracellular Potential
	Transmembrane	126 – 147	22	Helical; Name=3; Potential
	Topological domain	148 – 168	21	Cytoplasmic Potential
	Transmembrane	169 – 190	22	Helical; Name=4; Potential
	Topological domain	191 – 220	30	Extracellular Potential
	Transmembrane	221 – 241	21	Helical; Name=5; Potential
	Topological domain	242 – 299	58	Cytoplasmic Potential
	Transmembrane	300 – 319	20	Helical; Name=6; Potential
	Topological domain	320 – 337	18	Extracellular Potential
	Transmembrane	338 – 357	20	Helical; Name=7; Potential
	Topological domain	358 – 424	67	Cytoplasmic Potential
Amino acid modifications
	Modified residue	410	1	Phosphoserine By similarity
	Lipidation	371	1	S-palmitoyl cysteine Ref.9
	Lipidation	372	1	S-palmitoyl cysteine Ref.9
	Glycosylation	27	1	N-linked (GlcNAc...) Potential
	Disulfide bond	124 ↔ 205		By similarity
Experimental info
	Mutagenesis	14	1	N → Q: Reduced ligand binding affinity. Ref.8
	Mutagenesis	27	1	N → Q: Reduced ligand binding affinity. Ref.8
	Mutagenesis	115	1	Y → D: No significant change in affinity for arginine vasopressin or oxytocin. Increased affinity for 1-deamino, D-Arg8-vasopressin. Ref.6
	Mutagenesis	115	1	Y → F: 17-fold increase in affinity for oxytocin. No change in affinity for arginine vasopressin or 1-deamino, D-Arg8-vasopressin. Ref.6
	Mutagenesis	115	1	Y → L: 50-fold decrease in affinity for arginine vasopressin. Little affect on affinity for oxytocin or 1-deamino, D-Arg8-vasopressin. Ref.6
	Mutagenesis	371	1	C → G: Reduced palmitoylation, coupling to G protein unaffected. Abolished isoprenylation, coupling to G protein unaffected; when associated with G-372. Ref.9
	Mutagenesis	372	1	C → G: Reduced palmitoylation, coupling to G protein unaffected. Abolished isoprenylation, coupling to G protein unaffected; when associated with G-371. Ref.9
	Sequence conflict	115 – 116	2	YR → SS Ref.3
	Sequence conflict	115 – 116	2	YR → SS Ref.5
	Sequence conflict	390 – 424	35	RQTSY…IPVST → KTDFLF in CAA77748. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P30560 [UniParc]. Last modified June 13, 2006. Version 4. Checksum: 4F452C8DF7B4C900	FASTA	424	47,655
	10 20 30 40 50 60 MSFPRGSQDR SVGNSSPWWP LTTEGSNGSQ EAARLGEGDS PLGDVRNEEL AKLEIAVLAV 70 80 90 100 110 120 IFVVAVLGNS SVLLALHRTP RKTSRMHLFI RHLSLADLAV AFFQVLPQLC WDITYRFRGP 130 140 150 160 170 180 DWLCRVVKHL QVFAMFASAY MLVVMTADRY IAVCHPLKTL QQPARRSRLM IATSWVLSFI 190 200 210 220 230 240 LSTPQYFIFS VIEIEVNNGT KTQDCWATFI QPWGTRAYVT WMTSGVFVAP VVVLGTCYGF 250 260 270 280 290 300 ICYHIWRNIR GKTASSRHSK GDKGSGEAVG PFHKGLLVTP CVSSVKSISR AKIRTVKMTF 310 320 330 340 350 360 VIVSAYILCW APFFIVQMWS VWDENFIWTD SENPSITITA LLASLNSCCN PWIYMFFSGH 370 380 390 400 410 420 LLQDCVQSFP CCHSMAQKFA KDDSDSMSRR QTSYSNNRSP TNSTGMWKDS PKSSKSIRFI PVST

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and expression of a rat V1a arginine vasopressin receptor." Morel A., O'Carroll A.-M., Brownstein M.J., Lolait S.J. Nature 356:523-526(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Liver.
[2]	"Homology between neurohypophyseal hormone receptors." Wheatley M., Howl J., Morel A., Davis A.R.L. Biochem. J. 296:519-519(1993) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION TO 112-117.
[3]	"Sequence identity between the rat and human vasopressin V1a receptors." Innamorati G., Lolait S.J., Birnbaumer M. Biochem. J. 314:710-711(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Liver.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver.
[5]	"Molecular cloning and expression of rat V1a and V2 arginine vasopressin receptors." Morel A., Lolait S.J., Brownstein M.J. Regul. Pept. 45:53-59(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-395.
[6]	"Tyr115 is the key residue for determining agonist selectivity in the V1a vasopressin receptor." Chini B., Mouillac B., Ala Y., Balestre M.-N., Trumpp-Kallmeyer S., Hoflack J., Elands J., Hibert M., Manning M., Jard S., Barberis C. EMBO J. 14:2176-2182(1995) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS OF TYR-115.
[7]	"Localization of V1a vasopressin receptor mRNA expression in cultured neurons, astroglia, and oligodendroglia of rat cerebral cortex." Yamazaki R.S., Chen Q., Schreiber S.S., Brinton R.D. Brain Res. Mol. Brain Res. 45:138-140(1997) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[8]	"Effect of N-glycosylation on ligand binding affinity of rat V1a vasopressin receptor." Lee K.-H., Ahn J., Yu D.-H., Jeong H.-S., Lee S.-H., Kim K.-S., Chung I.-Y., Kim J.-H., Lee Y.-S. Biochem. Biophys. Res. Commun. 286:707-713(2001) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS OF ASN-14 AND ASN-27, SUBCELLULAR LOCATION.
[9]	"Palmitoylation of the vasopressin V1a receptor reveals different conformational requirements for signaling, agonist-induced receptor phosphorylation, and sequestration." Hawtin S.R., Tobin A.B., Patel S., Wheatley M. J. Biol. Chem. 276:38139-38146(2001) [PubMed] [Europe PMC] [Abstract] Cited for: PALMITOYLATION AT CYS-371 AND CYS-372, MUTAGENESIS OF CYS-371 AND CYS-372.
[10]	"Immunocytochemical localization of vasopressin v1a receptors in the rat pituitary gonadotropes." Orcel H., Tobin V.A., Alonso G., Rabie A. Endocrinology 143:4385-4388(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[11]	"Viral vector-mediated gene transfer of the vole V1a vasopressin receptor in the rat septum: improved social discrimination and active social behaviour." Landgraf R., Frank E., Aldag J.M., Neumann I.D., Sharer C.A., Ren X., Terwilliger E.F., Niwa M., Wigger A., Young L.J. Eur. J. Neurosci. 18:403-411(2003) [PubMed] [Europe PMC] [Abstract] Cited for: ROLE IN SOCIAL BEHAVIOR.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z11690 mRNA. Translation: CAA77748.1. Different initiation. U39450 Genomic DNA. Translation: AAC52507.1. BC088095 Genomic DNA. Translation: AAH88095.1.
IPI	IPI00210117.
PIR	S71837.
RefSeq	NP_444178.2. NM_053019.2.
UniGene	Rn.32282.
3D structure databases
ProteinModelPortal	P30560.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P30560.
Proteomic databases
PRIDE	P30560.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000005829; ENSRNOP00000005829; ENSRNOG00000004400.
GeneID	25107.
KEGG	rno:25107.
Organism-specific databases
CTD	552.
RGD	2185. Avpr1a.
Phylogenomic databases
eggNOG	NOG299951.
GeneTree	ENSGT00660000095384.
HOGENOM	HOG000237331.
HOVERGEN	HBG105710.
InParanoid	P30560.
KO	K04226.
OMA	QLCWDIT.
OrthoDB	EOG4KSPJT.
Gene expression databases
Genevestigator	P30560.
GermOnline	ENSRNOG00000004400. Rattus norvegicus.
Family and domain databases
InterPro	IPR015076. DUF1856. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR001817. Vasoprsn_rcpt. IPR001224. Vprs_V1A_rcpt. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. PF08983. DUF1856. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00896. VASOPRESSINR. PR00752. VASOPRSNV1AR.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P30560.
ChEMBL	CHEMBL2868.
NextBio	605443.

Entry information

Entry name

V1AR_RAT

Accession

Primary (citable) accession number: P30560
Secondary accession number(s): Q5M8D1, Q62874, Q9QW18

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	June 13, 2006
Last modified:	April 3, 2013
This is version 113 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program