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P30560

- V1AR_RAT

UniProt

P30560 - V1AR_RAT

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Protein

Vasopressin V1a receptor

Gene
Avpr1a
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Receptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which activate a phosphatidyl-inositol-calcium second messenger system. Involved in social memory formation.1 Publication

GO - Molecular functioni

  1. peptide hormone binding Source: RGD
  2. V1A vasopressin receptor binding Source: UniProtKB
  3. vasopressin receptor activity Source: RGD

GO - Biological processi

  1. calcium-mediated signaling Source: RGD
  2. cellular response to water deprivation Source: RGD
  3. G-protein coupled receptor signaling pathway Source: UniProtKB
  4. grooming behavior Source: RGD
  5. maternal aggressive behavior Source: RGD
  6. maternal behavior Source: RGD
  7. myotube differentiation Source: RGD
  8. negative regulation of female receptivity Source: RGD
  9. negative regulation of transmission of nerve impulse Source: RGD
  10. penile erection Source: RGD
  11. positive regulation of blood pressure Source: RGD
  12. positive regulation of cell growth Source: RGD
  13. positive regulation of cell proliferation Source: RGD
  14. positive regulation of cellular pH reduction Source: RGD
  15. positive regulation of cytosolic calcium ion concentration Source: RGD
  16. positive regulation of glutamate secretion Source: RGD
  17. positive regulation of glycogen catabolic process Source: UniProtKB
  18. positive regulation of heart rate Source: RGD
  19. positive regulation of prostaglandin biosynthetic process Source: RGD
  20. positive regulation of renal sodium excretion Source: RGD
  21. positive regulation of systemic arterial blood pressure Source: RGD
  22. positive regulation of urine volume Source: UniProtKB
  23. positive regulation of vasoconstriction Source: RGD
  24. regulation of corticotropin secretion Source: UniProtKB
  25. regulation of systemic arterial blood pressure by vasopressin Source: RGD
  26. response to corticosterone Source: RGD
  27. response to inorganic substance Source: RGD
  28. response to organic substance Source: RGD
  29. social behavior Source: UniProtKB
  30. sperm ejaculation Source: RGD
  31. telencephalon development Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
Vasopressin V1a receptor
Short name:
V1aR
Alternative name(s):
AVPR V1a
Antidiuretic hormone receptor 1a
Vascular/hepatic-type arginine vasopressin receptor
Gene namesi
Name:Avpr1a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 7

Organism-specific databases

RGDi2185. Avpr1a.

Subcellular locationi

Cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein
Note: Located in cytoplasmic vesicles dispersed throughout the cell cytoplasm and to the plasma membrane.2 Publications

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5252Extracellular Reviewed predictionAdd
BLAST
Transmembranei53 – 7624Helical; Name=1; Reviewed predictionAdd
BLAST
Topological domaini77 – 8812Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei89 – 11022Helical; Name=2; Reviewed predictionAdd
BLAST
Topological domaini111 – 12515Extracellular Reviewed predictionAdd
BLAST
Transmembranei126 – 14722Helical; Name=3; Reviewed predictionAdd
BLAST
Topological domaini148 – 16821Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei169 – 19022Helical; Name=4; Reviewed predictionAdd
BLAST
Topological domaini191 – 22030Extracellular Reviewed predictionAdd
BLAST
Transmembranei221 – 24121Helical; Name=5; Reviewed predictionAdd
BLAST
Topological domaini242 – 29958Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei300 – 31920Helical; Name=6; Reviewed predictionAdd
BLAST
Topological domaini320 – 33718Extracellular Reviewed predictionAdd
BLAST
Transmembranei338 – 35720Helical; Name=7; Reviewed predictionAdd
BLAST
Topological domaini358 – 42467Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. cytoplasmic vesicle Source: RGD
  2. cytoplasmic vesicle membrane Source: UniProtKB-SubCell
  3. integral component of membrane Source: UniProtKB
  4. plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi14 – 141N → Q: Reduced ligand binding affinity. 1 Publication
Mutagenesisi27 – 271N → Q: Reduced ligand binding affinity. 1 Publication
Mutagenesisi115 – 1151Y → D: No significant change in affinity for arginine vasopressin or oxytocin. Increased affinity for 1-deamino, D-Arg8-vasopressin. 1 Publication
Mutagenesisi115 – 1151Y → F: 17-fold increase in affinity for oxytocin. No change in affinity for arginine vasopressin or 1-deamino, D-Arg8-vasopressin. 1 Publication
Mutagenesisi115 – 1151Y → L: 50-fold decrease in affinity for arginine vasopressin. Little affect on affinity for oxytocin or 1-deamino, D-Arg8-vasopressin. 1 Publication
Mutagenesisi371 – 3711C → G: Reduced palmitoylation, coupling to G protein unaffected. Abolished isoprenylation, coupling to G protein unaffected; when associated with G-372. 1 Publication
Mutagenesisi372 – 3721C → G: Reduced palmitoylation, coupling to G protein unaffected. Abolished isoprenylation, coupling to G protein unaffected; when associated with G-371. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 424424Vasopressin V1a receptorPRO_0000070201Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi27 – 271N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi124 ↔ 205 By similarity
Lipidationi371 – 3711S-palmitoyl cysteine1 Publication
Lipidationi372 – 3721S-palmitoyl cysteine1 Publication
Modified residuei410 – 4101Phosphoserine By similarity

Post-translational modificationi

Palmitoylated on three cysteine residues, of which only two are identified.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PRIDEiP30560.

PTM databases

PhosphoSiteiP30560.

Expressioni

Tissue specificityi

Localized within gonadotropes of the anterior pituitary of the brain. Broadly distributed throughout the cerebral cortex.2 Publications

Gene expression databases

GenevestigatoriP30560.

Interactioni

Protein-protein interaction databases

MINTiMINT-4996637.

Structurei

3D structure databases

ProteinModelPortaliP30560.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG299951.
GeneTreeiENSGT00660000095384.
HOGENOMiHOG000237331.
HOVERGENiHBG105710.
InParanoidiP30560.
KOiK04226.
OMAiQLCWDIT.
OrthoDBiEOG7Z69CP.
PhylomeDBiP30560.
TreeFamiTF106499.

Family and domain databases

Gene3Di1.20.1070.10. 1 hit.
InterProiIPR015076. DUF1856.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001817. Vasoprsn_rcpt.
IPR001224. Vprs_V1A_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
PF08983. DUF1856. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00896. VASOPRESSINR.
PR00752. VASOPRSNV1AR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P30560-1 [UniParc]FASTAAdd to Basket

« Hide

MSFPRGSQDR SVGNSSPWWP LTTEGSNGSQ EAARLGEGDS PLGDVRNEEL    50
AKLEIAVLAV IFVVAVLGNS SVLLALHRTP RKTSRMHLFI RHLSLADLAV 100
AFFQVLPQLC WDITYRFRGP DWLCRVVKHL QVFAMFASAY MLVVMTADRY 150
IAVCHPLKTL QQPARRSRLM IATSWVLSFI LSTPQYFIFS VIEIEVNNGT 200
KTQDCWATFI QPWGTRAYVT WMTSGVFVAP VVVLGTCYGF ICYHIWRNIR 250
GKTASSRHSK GDKGSGEAVG PFHKGLLVTP CVSSVKSISR AKIRTVKMTF 300
VIVSAYILCW APFFIVQMWS VWDENFIWTD SENPSITITA LLASLNSCCN 350
PWIYMFFSGH LLQDCVQSFP CCHSMAQKFA KDDSDSMSRR QTSYSNNRSP 400
TNSTGMWKDS PKSSKSIRFI PVST 424
Length:424
Mass (Da):47,655
Last modified:June 13, 2006 - v4
Checksum:i4F452C8DF7B4C900
GO

Sequence cautioni

The sequence CAA77748.1 differs from that shown. Reason: Erroneous initiation.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti115 – 1162YR → SS1 Publication
Sequence conflicti115 – 1162YR → SS1 Publication
Sequence conflicti390 – 42435RQTSY…IPVST → KTDFLF in CAA77748. 1 PublicationAdd
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z11690 mRNA. Translation: CAA77748.1. Different initiation.
U39450 Genomic DNA. Translation: AAC52507.1.
BC088095 Genomic DNA. Translation: AAH88095.1.
PIRiS71837.
RefSeqiNP_444178.2. NM_053019.2.
UniGeneiRn.32282.

Genome annotation databases

EnsembliENSRNOT00000005829; ENSRNOP00000005829; ENSRNOG00000004400.
GeneIDi25107.
KEGGirno:25107.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z11690 mRNA. Translation: CAA77748.1 . Different initiation.
U39450 Genomic DNA. Translation: AAC52507.1 .
BC088095 Genomic DNA. Translation: AAH88095.1 .
PIRi S71837.
RefSeqi NP_444178.2. NM_053019.2.
UniGenei Rn.32282.

3D structure databases

ProteinModelPortali P30560.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-4996637.

Chemistry

BindingDBi P30560.
ChEMBLi CHEMBL2868.
GuidetoPHARMACOLOGYi 366.

Protein family/group databases

GPCRDBi Search...

PTM databases

PhosphoSitei P30560.

Proteomic databases

PRIDEi P30560.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000005829 ; ENSRNOP00000005829 ; ENSRNOG00000004400 .
GeneIDi 25107.
KEGGi rno:25107.

Organism-specific databases

CTDi 552.
RGDi 2185. Avpr1a.

Phylogenomic databases

eggNOGi NOG299951.
GeneTreei ENSGT00660000095384.
HOGENOMi HOG000237331.
HOVERGENi HBG105710.
InParanoidi P30560.
KOi K04226.
OMAi QLCWDIT.
OrthoDBi EOG7Z69CP.
PhylomeDBi P30560.
TreeFami TF106499.

Miscellaneous databases

NextBioi 605443.
PROi P30560.

Gene expression databases

Genevestigatori P30560.

Family and domain databases

Gene3Di 1.20.1070.10. 1 hit.
InterProi IPR015076. DUF1856.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001817. Vasoprsn_rcpt.
IPR001224. Vprs_V1A_rcpt.
[Graphical view ]
Pfami PF00001. 7tm_1. 1 hit.
PF08983. DUF1856. 1 hit.
[Graphical view ]
PRINTSi PR00237. GPCRRHODOPSN.
PR00896. VASOPRESSINR.
PR00752. VASOPRSNV1AR.
PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and expression of a rat V1a arginine vasopressin receptor."
    Morel A., O'Carroll A.-M., Brownstein M.J., Lolait S.J.
    Nature 356:523-526(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Liver.
  2. "Homology between neurohypophyseal hormone receptors."
    Wheatley M., Howl J., Morel A., Davis A.R.L.
    Biochem. J. 296:519-519(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION TO 112-117.
  3. "Sequence identity between the rat and human vasopressin V1a receptors."
    Innamorati G., Lolait S.J., Birnbaumer M.
    Biochem. J. 314:710-711(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Liver.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.
  5. "Molecular cloning and expression of rat V1a and V2 arginine vasopressin receptors."
    Morel A., Lolait S.J., Brownstein M.J.
    Regul. Pept. 45:53-59(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-395.
  6. "Tyr115 is the key residue for determining agonist selectivity in the V1a vasopressin receptor."
    Chini B., Mouillac B., Ala Y., Balestre M.-N., Trumpp-Kallmeyer S., Hoflack J., Elands J., Hibert M., Manning M., Jard S., Barberis C.
    EMBO J. 14:2176-2182(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF TYR-115.
  7. "Localization of V1a vasopressin receptor mRNA expression in cultured neurons, astroglia, and oligodendroglia of rat cerebral cortex."
    Yamazaki R.S., Chen Q., Schreiber S.S., Brinton R.D.
    Brain Res. Mol. Brain Res. 45:138-140(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  8. "Effect of N-glycosylation on ligand binding affinity of rat V1a vasopressin receptor."
    Lee K.-H., Ahn J., Yu D.-H., Jeong H.-S., Lee S.-H., Kim K.-S., Chung I.-Y., Kim J.-H., Lee Y.-S.
    Biochem. Biophys. Res. Commun. 286:707-713(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF ASN-14 AND ASN-27, SUBCELLULAR LOCATION.
  9. "Palmitoylation of the vasopressin V1a receptor reveals different conformational requirements for signaling, agonist-induced receptor phosphorylation, and sequestration."
    Hawtin S.R., Tobin A.B., Patel S., Wheatley M.
    J. Biol. Chem. 276:38139-38146(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: PALMITOYLATION AT CYS-371 AND CYS-372, MUTAGENESIS OF CYS-371 AND CYS-372.
  10. "Immunocytochemical localization of vasopressin v1a receptors in the rat pituitary gonadotropes."
    Orcel H., Tobin V.A., Alonso G., Rabie A.
    Endocrinology 143:4385-4388(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  11. "Viral vector-mediated gene transfer of the vole V1a vasopressin receptor in the rat septum: improved social discrimination and active social behaviour."
    Landgraf R., Frank E., Aldag J.M., Neumann I.D., Sharer C.A., Ren X., Terwilliger E.F., Niwa M., Wigger A., Young L.J.
    Eur. J. Neurosci. 18:403-411(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: ROLE IN SOCIAL BEHAVIOR.

Entry informationi

Entry nameiV1AR_RAT
AccessioniPrimary (citable) accession number: P30560
Secondary accession number(s): Q5M8D1, Q62874, Q9QW18
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: June 13, 2006
Last modified: June 11, 2014
This is version 122 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Overexpression of AVPR1A in the brain increases the duration of social memory.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi