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P30560

- V1AR_RAT

UniProt

P30560 - V1AR_RAT

Protein

Vasopressin V1a receptor

Gene

Avpr1a

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 4 (13 Jun 2006)
      Previous versions | rss
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    Functioni

    Receptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which activate a phosphatidyl-inositol-calcium second messenger system. Involved in social memory formation.1 Publication

    GO - Molecular functioni

    1. peptide hormone binding Source: RGD
    2. V1A vasopressin receptor binding Source: UniProtKB
    3. vasopressin receptor activity Source: RGD

    GO - Biological processi

    1. calcium-mediated signaling Source: RGD
    2. cellular response to water deprivation Source: RGD
    3. G-protein coupled receptor signaling pathway Source: UniProtKB
    4. grooming behavior Source: RGD
    5. maternal aggressive behavior Source: RGD
    6. maternal behavior Source: RGD
    7. myotube differentiation Source: RGD
    8. negative regulation of female receptivity Source: RGD
    9. negative regulation of transmission of nerve impulse Source: RGD
    10. penile erection Source: RGD
    11. positive regulation of blood pressure Source: RGD
    12. positive regulation of cell growth Source: RGD
    13. positive regulation of cell proliferation Source: RGD
    14. positive regulation of cellular pH reduction Source: RGD
    15. positive regulation of cytosolic calcium ion concentration Source: RGD
    16. positive regulation of glutamate secretion Source: RGD
    17. positive regulation of glycogen catabolic process Source: UniProtKB
    18. positive regulation of heart rate Source: RGD
    19. positive regulation of prostaglandin biosynthetic process Source: RGD
    20. positive regulation of renal sodium excretion Source: RGD
    21. positive regulation of systemic arterial blood pressure Source: RGD
    22. positive regulation of urine volume Source: UniProtKB
    23. positive regulation of vasoconstriction Source: RGD
    24. regulation of corticotropin secretion Source: UniProtKB
    25. regulation of systemic arterial blood pressure by vasopressin Source: RGD
    26. response to corticosterone Source: RGD
    27. response to inorganic substance Source: RGD
    28. response to organic substance Source: RGD
    29. social behavior Source: UniProtKB
    30. sperm ejaculation Source: RGD
    31. telencephalon development Source: RGD

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vasopressin V1a receptor
    Short name:
    V1aR
    Alternative name(s):
    AVPR V1a
    Antidiuretic hormone receptor 1a
    Vascular/hepatic-type arginine vasopressin receptor
    Gene namesi
    Name:Avpr1a
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 7

    Organism-specific databases

    RGDi2185. Avpr1a.

    Subcellular locationi

    Cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein
    Note: Located in cytoplasmic vesicles dispersed throughout the cell cytoplasm and to the plasma membrane.

    GO - Cellular componenti

    1. cytoplasmic vesicle Source: RGD
    2. cytoplasmic vesicle membrane Source: UniProtKB-SubCell
    3. integral component of membrane Source: UniProtKB
    4. plasma membrane Source: RGD

    Keywords - Cellular componenti

    Cell membrane, Cytoplasmic vesicle, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi14 – 141N → Q: Reduced ligand binding affinity. 1 Publication
    Mutagenesisi27 – 271N → Q: Reduced ligand binding affinity. 1 Publication
    Mutagenesisi115 – 1151Y → D: No significant change in affinity for arginine vasopressin or oxytocin. Increased affinity for 1-deamino, D-Arg8-vasopressin. 1 Publication
    Mutagenesisi115 – 1151Y → F: 17-fold increase in affinity for oxytocin. No change in affinity for arginine vasopressin or 1-deamino, D-Arg8-vasopressin. 1 Publication
    Mutagenesisi115 – 1151Y → L: 50-fold decrease in affinity for arginine vasopressin. Little affect on affinity for oxytocin or 1-deamino, D-Arg8-vasopressin. 1 Publication
    Mutagenesisi371 – 3711C → G: Reduced palmitoylation, coupling to G protein unaffected. Abolished isoprenylation, coupling to G protein unaffected; when associated with G-372. 1 Publication
    Mutagenesisi372 – 3721C → G: Reduced palmitoylation, coupling to G protein unaffected. Abolished isoprenylation, coupling to G protein unaffected; when associated with G-371. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 424424Vasopressin V1a receptorPRO_0000070201Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi27 – 271N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi124 ↔ 205PROSITE-ProRule annotation
    Lipidationi371 – 3711S-palmitoyl cysteine1 Publication
    Lipidationi372 – 3721S-palmitoyl cysteine1 Publication
    Modified residuei410 – 4101PhosphoserineBy similarity

    Post-translational modificationi

    Palmitoylated on three cysteine residues, of which only two are identified.1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

    Proteomic databases

    PRIDEiP30560.

    PTM databases

    PhosphoSiteiP30560.

    Expressioni

    Tissue specificityi

    Localized within gonadotropes of the anterior pituitary of the brain. Broadly distributed throughout the cerebral cortex.2 Publications

    Gene expression databases

    GenevestigatoriP30560.

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-4996637.

    Structurei

    3D structure databases

    ProteinModelPortaliP30560.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 5252ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini77 – 8812CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini111 – 12515ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini148 – 16821CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini191 – 22030ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini242 – 29958CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini320 – 33718ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini358 – 42467CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei53 – 7624Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei89 – 11022Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei126 – 14722Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei169 – 19022Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei221 – 24121Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei300 – 31920Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei338 – 35720Helical; Name=7Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family. Vasopressin/oxytocin receptor subfamily.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG299951.
    GeneTreeiENSGT00660000095384.
    HOGENOMiHOG000237331.
    HOVERGENiHBG105710.
    InParanoidiP30560.
    KOiK04226.
    OMAiQLCWDIT.
    OrthoDBiEOG7Z69CP.
    PhylomeDBiP30560.
    TreeFamiTF106499.

    Family and domain databases

    Gene3Di1.20.1070.10. 1 hit.
    InterProiIPR015076. DUF1856.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR001817. Vasoprsn_rcpt.
    IPR001224. Vprs_V1A_rcpt.
    [Graphical view]
    PfamiPF00001. 7tm_1. 1 hit.
    PF08983. DUF1856. 1 hit.
    [Graphical view]
    PRINTSiPR00237. GPCRRHODOPSN.
    PR00896. VASOPRESSINR.
    PR00752. VASOPRSNV1AR.
    PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P30560-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSFPRGSQDR SVGNSSPWWP LTTEGSNGSQ EAARLGEGDS PLGDVRNEEL    50
    AKLEIAVLAV IFVVAVLGNS SVLLALHRTP RKTSRMHLFI RHLSLADLAV 100
    AFFQVLPQLC WDITYRFRGP DWLCRVVKHL QVFAMFASAY MLVVMTADRY 150
    IAVCHPLKTL QQPARRSRLM IATSWVLSFI LSTPQYFIFS VIEIEVNNGT 200
    KTQDCWATFI QPWGTRAYVT WMTSGVFVAP VVVLGTCYGF ICYHIWRNIR 250
    GKTASSRHSK GDKGSGEAVG PFHKGLLVTP CVSSVKSISR AKIRTVKMTF 300
    VIVSAYILCW APFFIVQMWS VWDENFIWTD SENPSITITA LLASLNSCCN 350
    PWIYMFFSGH LLQDCVQSFP CCHSMAQKFA KDDSDSMSRR QTSYSNNRSP 400
    TNSTGMWKDS PKSSKSIRFI PVST 424
    Length:424
    Mass (Da):47,655
    Last modified:June 13, 2006 - v4
    Checksum:i4F452C8DF7B4C900
    GO

    Sequence cautioni

    The sequence CAA77748.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti115 – 1162YR → SS(PubMed:8670090)Curated
    Sequence conflicti115 – 1162YR → SS(PubMed:8511367)Curated
    Sequence conflicti390 – 42435RQTSY…IPVST → KTDFLF in CAA77748. (PubMed:1560825)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z11690 mRNA. Translation: CAA77748.1. Different initiation.
    U39450 Genomic DNA. Translation: AAC52507.1.
    BC088095 Genomic DNA. Translation: AAH88095.1.
    PIRiS71837.
    RefSeqiNP_444178.2. NM_053019.2.
    UniGeneiRn.32282.

    Genome annotation databases

    EnsembliENSRNOT00000005829; ENSRNOP00000005829; ENSRNOG00000004400.
    GeneIDi25107.
    KEGGirno:25107.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z11690 mRNA. Translation: CAA77748.1 . Different initiation.
    U39450 Genomic DNA. Translation: AAC52507.1 .
    BC088095 Genomic DNA. Translation: AAH88095.1 .
    PIRi S71837.
    RefSeqi NP_444178.2. NM_053019.2.
    UniGenei Rn.32282.

    3D structure databases

    ProteinModelPortali P30560.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-4996637.

    Chemistry

    BindingDBi P30560.
    ChEMBLi CHEMBL2868.
    GuidetoPHARMACOLOGYi 366.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei P30560.

    Proteomic databases

    PRIDEi P30560.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000005829 ; ENSRNOP00000005829 ; ENSRNOG00000004400 .
    GeneIDi 25107.
    KEGGi rno:25107.

    Organism-specific databases

    CTDi 552.
    RGDi 2185. Avpr1a.

    Phylogenomic databases

    eggNOGi NOG299951.
    GeneTreei ENSGT00660000095384.
    HOGENOMi HOG000237331.
    HOVERGENi HBG105710.
    InParanoidi P30560.
    KOi K04226.
    OMAi QLCWDIT.
    OrthoDBi EOG7Z69CP.
    PhylomeDBi P30560.
    TreeFami TF106499.

    Miscellaneous databases

    NextBioi 605443.
    PROi P30560.

    Gene expression databases

    Genevestigatori P30560.

    Family and domain databases

    Gene3Di 1.20.1070.10. 1 hit.
    InterProi IPR015076. DUF1856.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR001817. Vasoprsn_rcpt.
    IPR001224. Vprs_V1A_rcpt.
    [Graphical view ]
    Pfami PF00001. 7tm_1. 1 hit.
    PF08983. DUF1856. 1 hit.
    [Graphical view ]
    PRINTSi PR00237. GPCRRHODOPSN.
    PR00896. VASOPRESSINR.
    PR00752. VASOPRSNV1AR.
    PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and expression of a rat V1a arginine vasopressin receptor."
      Morel A., O'Carroll A.-M., Brownstein M.J., Lolait S.J.
      Nature 356:523-526(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Liver.
    2. "Homology between neurohypophyseal hormone receptors."
      Wheatley M., Howl J., Morel A., Davis A.R.L.
      Biochem. J. 296:519-519(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: SEQUENCE REVISION TO 112-117.
    3. "Sequence identity between the rat and human vasopressin V1a receptors."
      Innamorati G., Lolait S.J., Birnbaumer M.
      Biochem. J. 314:710-711(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Tissue: Liver.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Liver.
    5. "Molecular cloning and expression of rat V1a and V2 arginine vasopressin receptors."
      Morel A., Lolait S.J., Brownstein M.J.
      Regul. Pept. 45:53-59(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-395.
    6. "Tyr115 is the key residue for determining agonist selectivity in the V1a vasopressin receptor."
      Chini B., Mouillac B., Ala Y., Balestre M.-N., Trumpp-Kallmeyer S., Hoflack J., Elands J., Hibert M., Manning M., Jard S., Barberis C.
      EMBO J. 14:2176-2182(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF TYR-115.
    7. "Localization of V1a vasopressin receptor mRNA expression in cultured neurons, astroglia, and oligodendroglia of rat cerebral cortex."
      Yamazaki R.S., Chen Q., Schreiber S.S., Brinton R.D.
      Brain Res. Mol. Brain Res. 45:138-140(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    8. "Effect of N-glycosylation on ligand binding affinity of rat V1a vasopressin receptor."
      Lee K.-H., Ahn J., Yu D.-H., Jeong H.-S., Lee S.-H., Kim K.-S., Chung I.-Y., Kim J.-H., Lee Y.-S.
      Biochem. Biophys. Res. Commun. 286:707-713(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF ASN-14 AND ASN-27, SUBCELLULAR LOCATION.
    9. "Palmitoylation of the vasopressin V1a receptor reveals different conformational requirements for signaling, agonist-induced receptor phosphorylation, and sequestration."
      Hawtin S.R., Tobin A.B., Patel S., Wheatley M.
      J. Biol. Chem. 276:38139-38146(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: PALMITOYLATION AT CYS-371 AND CYS-372, MUTAGENESIS OF CYS-371 AND CYS-372.
    10. "Immunocytochemical localization of vasopressin v1a receptors in the rat pituitary gonadotropes."
      Orcel H., Tobin V.A., Alonso G., Rabie A.
      Endocrinology 143:4385-4388(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    11. "Viral vector-mediated gene transfer of the vole V1a vasopressin receptor in the rat septum: improved social discrimination and active social behaviour."
      Landgraf R., Frank E., Aldag J.M., Neumann I.D., Sharer C.A., Ren X., Terwilliger E.F., Niwa M., Wigger A., Young L.J.
      Eur. J. Neurosci. 18:403-411(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: ROLE IN SOCIAL BEHAVIOR.

    Entry informationi

    Entry nameiV1AR_RAT
    AccessioniPrimary (citable) accession number: P30560
    Secondary accession number(s): Q5M8D1, Q62874, Q9QW18
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: June 13, 2006
    Last modified: October 1, 2014
    This is version 123 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Overexpression of AVPR1A in the brain increases the duration of social memory.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3