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P30560 (V1AR_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vasopressin V1a receptor

Short name=V1aR
Alternative name(s):
AVPR V1a
Antidiuretic hormone receptor 1a
Vascular/hepatic-type arginine vasopressin receptor
Gene names
Name:Avpr1a
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length424 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which activate a phosphatidyl-inositol-calcium second messenger system. Involved in social memory formation. Ref.11

Subcellular location

Cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein. Note: Located in cytoplasmic vesicles dispersed throughout the cell cytoplasm and to the plasma membrane. Ref.8 Ref.10

Tissue specificity

Localized within gonadotropes of the anterior pituitary of the brain. Broadly distributed throughout the cerebral cortex. Ref.7 Ref.10

Post-translational modification

Palmitoylated on three cysteine residues, of which only two are identified. Ref.9

Miscellaneous

Overexpression of AVPR1A in the brain increases the duration of social memory.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family. Vasopressin/oxytocin receptor subfamily.

Sequence caution

The sequence CAA77748.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Cellular componentCell membrane
Cytoplasmic vesicle
Membrane
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
Lipoprotein
Palmitate
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processG-protein coupled receptor signaling pathway

Non-traceable author statement Ref.1. Source: UniProtKB

calcium-mediated signaling

Inferred from mutant phenotype PubMed 12088846. Source: RGD

cellular response to water deprivation

Inferred from expression pattern PubMed 11796498. Source: RGD

grooming behavior

Inferred from mutant phenotype PubMed 18640147. Source: RGD

maternal aggressive behavior

Inferred from mutant phenotype PubMed 18640147. Source: RGD

maternal behavior

Inferred from mutant phenotype PubMed 18640147. Source: RGD

myotube differentiation

Inferred from expression pattern PubMed 18021262. Source: RGD

negative regulation of female receptivity

Inferred from mutant phenotype PubMed 16488546. Source: RGD

negative regulation of transmission of nerve impulse

Inferred from mutant phenotype PubMed 16304841. Source: RGD

penile erection

Inferred from mutant phenotype PubMed 18552879. Source: RGD

positive regulation of blood pressure

Inferred from mutant phenotype PubMed 18339407. Source: RGD

positive regulation of cell growth

Inferred from mutant phenotype PubMed 17395547. Source: RGD

positive regulation of cell proliferation

Inferred from mutant phenotype PubMed 17395547. Source: RGD

positive regulation of cellular pH reduction

Inferred from mutant phenotype PubMed 17762181. Source: RGD

positive regulation of cytosolic calcium ion concentration

Inferred from mutant phenotype PubMed 12088846. Source: RGD

positive regulation of glutamate secretion

Inferred from mutant phenotype PubMed 17877638. Source: RGD

positive regulation of glycogen catabolic process

Non-traceable author statement Ref.1. Source: UniProtKB

positive regulation of heart rate

Inferred from mutant phenotype PubMed 18339407. Source: RGD

positive regulation of prostaglandin biosynthetic process

Inferred from mutant phenotype PubMed 17254553. Source: RGD

positive regulation of renal sodium excretion

Inferred from mutant phenotype PubMed 18596120. Source: RGD

positive regulation of systemic arterial blood pressure

Inferred from mutant phenotype PubMed 17350115PubMed 18957383. Source: RGD

positive regulation of urine volume

Non-traceable author statement Ref.1. Source: UniProtKB

positive regulation of vasoconstriction

Inferred from mutant phenotype PubMed 17428891. Source: RGD

regulation of corticotropin secretion

Non-traceable author statement Ref.1. Source: UniProtKB

regulation of systemic arterial blood pressure by vasopressin

Traceable author statement PubMed 12369733. Source: RGD

response to corticosterone

Inferred from expression pattern PubMed 15475662. Source: RGD

response to inorganic substance

Inferred from mutant phenotype PubMed 16861049. Source: RGD

response to organic substance

Inferred from mutant phenotype PubMed 16966841. Source: RGD

social behavior

Inferred from direct assay Ref.11. Source: UniProtKB

sperm ejaculation

Inferred from mutant phenotype PubMed 18552879. Source: RGD

telencephalon development

Inferred from expression pattern PubMed 11021977. Source: RGD

   Cellular_componentcytoplasmic vesicle

Inferred from direct assay PubMed 11796498. Source: RGD

cytoplasmic vesicle membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Non-traceable author statement Ref.1. Source: UniProtKB

plasma membrane

Inferred from direct assay PubMed 16049168. Source: RGD

   Molecular_functionV1A vasopressin receptor binding

Inferred from direct assay Ref.1. Source: UniProtKB

peptide hormone binding

Inferred from mutant phenotype PubMed 12942143. Source: RGD

vasopressin receptor activity

Inferred from direct assay PubMed 19560475. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 424424Vasopressin V1a receptor
PRO_0000070201

Regions

Topological domain1 – 5252Extracellular Potential
Transmembrane53 – 7624Helical; Name=1; Potential
Topological domain77 – 8812Cytoplasmic Potential
Transmembrane89 – 11022Helical; Name=2; Potential
Topological domain111 – 12515Extracellular Potential
Transmembrane126 – 14722Helical; Name=3; Potential
Topological domain148 – 16821Cytoplasmic Potential
Transmembrane169 – 19022Helical; Name=4; Potential
Topological domain191 – 22030Extracellular Potential
Transmembrane221 – 24121Helical; Name=5; Potential
Topological domain242 – 29958Cytoplasmic Potential
Transmembrane300 – 31920Helical; Name=6; Potential
Topological domain320 – 33718Extracellular Potential
Transmembrane338 – 35720Helical; Name=7; Potential
Topological domain358 – 42467Cytoplasmic Potential

Amino acid modifications

Modified residue4101Phosphoserine By similarity
Lipidation3711S-palmitoyl cysteine Ref.9
Lipidation3721S-palmitoyl cysteine Ref.9
Glycosylation271N-linked (GlcNAc...) Potential
Disulfide bond124 ↔ 205 By similarity

Experimental info

Mutagenesis141N → Q: Reduced ligand binding affinity. Ref.8
Mutagenesis271N → Q: Reduced ligand binding affinity. Ref.8
Mutagenesis1151Y → D: No significant change in affinity for arginine vasopressin or oxytocin. Increased affinity for 1-deamino, D-Arg8-vasopressin. Ref.6
Mutagenesis1151Y → F: 17-fold increase in affinity for oxytocin. No change in affinity for arginine vasopressin or 1-deamino, D-Arg8-vasopressin. Ref.6
Mutagenesis1151Y → L: 50-fold decrease in affinity for arginine vasopressin. Little affect on affinity for oxytocin or 1-deamino, D-Arg8-vasopressin. Ref.6
Mutagenesis3711C → G: Reduced palmitoylation, coupling to G protein unaffected. Abolished isoprenylation, coupling to G protein unaffected; when associated with G-372. Ref.9
Mutagenesis3721C → G: Reduced palmitoylation, coupling to G protein unaffected. Abolished isoprenylation, coupling to G protein unaffected; when associated with G-371. Ref.9
Sequence conflict115 – 1162YR → SS Ref.3
Sequence conflict115 – 1162YR → SS Ref.5
Sequence conflict390 – 42435RQTSY…IPVST → KTDFLF in CAA77748. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P30560 [UniParc].

Last modified June 13, 2006. Version 4.
Checksum: 4F452C8DF7B4C900

FASTA42447,655
        10         20         30         40         50         60 
MSFPRGSQDR SVGNSSPWWP LTTEGSNGSQ EAARLGEGDS PLGDVRNEEL AKLEIAVLAV 

        70         80         90        100        110        120 
IFVVAVLGNS SVLLALHRTP RKTSRMHLFI RHLSLADLAV AFFQVLPQLC WDITYRFRGP 

       130        140        150        160        170        180 
DWLCRVVKHL QVFAMFASAY MLVVMTADRY IAVCHPLKTL QQPARRSRLM IATSWVLSFI 

       190        200        210        220        230        240 
LSTPQYFIFS VIEIEVNNGT KTQDCWATFI QPWGTRAYVT WMTSGVFVAP VVVLGTCYGF 

       250        260        270        280        290        300 
ICYHIWRNIR GKTASSRHSK GDKGSGEAVG PFHKGLLVTP CVSSVKSISR AKIRTVKMTF 

       310        320        330        340        350        360 
VIVSAYILCW APFFIVQMWS VWDENFIWTD SENPSITITA LLASLNSCCN PWIYMFFSGH 

       370        380        390        400        410        420 
LLQDCVQSFP CCHSMAQKFA KDDSDSMSRR QTSYSNNRSP TNSTGMWKDS PKSSKSIRFI 


PVST 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and expression of a rat V1a arginine vasopressin receptor."
Morel A., O'Carroll A.-M., Brownstein M.J., Lolait S.J.
Nature 356:523-526(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Liver.
[2]"Homology between neurohypophyseal hormone receptors."
Wheatley M., Howl J., Morel A., Davis A.R.L.
Biochem. J. 296:519-519(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION TO 112-117.
[3]"Sequence identity between the rat and human vasopressin V1a receptors."
Innamorati G., Lolait S.J., Birnbaumer M.
Biochem. J. 314:710-711(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Liver.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Liver.
[5]"Molecular cloning and expression of rat V1a and V2 arginine vasopressin receptors."
Morel A., Lolait S.J., Brownstein M.J.
Regul. Pept. 45:53-59(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-395.
[6]"Tyr115 is the key residue for determining agonist selectivity in the V1a vasopressin receptor."
Chini B., Mouillac B., Ala Y., Balestre M.-N., Trumpp-Kallmeyer S., Hoflack J., Elands J., Hibert M., Manning M., Jard S., Barberis C.
EMBO J. 14:2176-2182(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF TYR-115.
[7]"Localization of V1a vasopressin receptor mRNA expression in cultured neurons, astroglia, and oligodendroglia of rat cerebral cortex."
Yamazaki R.S., Chen Q., Schreiber S.S., Brinton R.D.
Brain Res. Mol. Brain Res. 45:138-140(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[8]"Effect of N-glycosylation on ligand binding affinity of rat V1a vasopressin receptor."
Lee K.-H., Ahn J., Yu D.-H., Jeong H.-S., Lee S.-H., Kim K.-S., Chung I.-Y., Kim J.-H., Lee Y.-S.
Biochem. Biophys. Res. Commun. 286:707-713(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF ASN-14 AND ASN-27, SUBCELLULAR LOCATION.
[9]"Palmitoylation of the vasopressin V1a receptor reveals different conformational requirements for signaling, agonist-induced receptor phosphorylation, and sequestration."
Hawtin S.R., Tobin A.B., Patel S., Wheatley M.
J. Biol. Chem. 276:38139-38146(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: PALMITOYLATION AT CYS-371 AND CYS-372, MUTAGENESIS OF CYS-371 AND CYS-372.
[10]"Immunocytochemical localization of vasopressin v1a receptors in the rat pituitary gonadotropes."
Orcel H., Tobin V.A., Alonso G., Rabie A.
Endocrinology 143:4385-4388(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[11]"Viral vector-mediated gene transfer of the vole V1a vasopressin receptor in the rat septum: improved social discrimination and active social behaviour."
Landgraf R., Frank E., Aldag J.M., Neumann I.D., Sharer C.A., Ren X., Terwilliger E.F., Niwa M., Wigger A., Young L.J.
Eur. J. Neurosci. 18:403-411(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: ROLE IN SOCIAL BEHAVIOR.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z11690 mRNA. Translation: CAA77748.1. Different initiation.
U39450 Genomic DNA. Translation: AAC52507.1.
BC088095 Genomic DNA. Translation: AAH88095.1.
PIRS71837.
RefSeqNP_444178.2. NM_053019.2.
UniGeneRn.32282.

3D structure databases

ProteinModelPortalP30560.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-4996637.

Chemistry

BindingDBP30560.
ChEMBLCHEMBL2096622.
GuidetoPHARMACOLOGY366.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP30560.

Proteomic databases

PRIDEP30560.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000005829; ENSRNOP00000005829; ENSRNOG00000004400.
GeneID25107.
KEGGrno:25107.

Organism-specific databases

CTD552.
RGD2185. Avpr1a.

Phylogenomic databases

eggNOGNOG299951.
GeneTreeENSGT00660000095384.
HOGENOMHOG000237331.
HOVERGENHBG105710.
InParanoidP30560.
KOK04226.
OMAQLCWDIT.
OrthoDBEOG7Z69CP.
PhylomeDBP30560.
TreeFamTF106499.

Gene expression databases

GenevestigatorP30560.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR015076. DUF1856.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001817. Vasoprsn_rcpt.
IPR001224. Vprs_V1A_rcpt.
[Graphical view]
PfamPF00001. 7tm_1. 1 hit.
PF08983. DUF1856. 1 hit.
[Graphical view]
PRINTSPR00237. GPCRRHODOPSN.
PR00896. VASOPRESSINR.
PR00752. VASOPRSNV1AR.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio605443.
PROP30560.

Entry information

Entry nameV1AR_RAT
AccessionPrimary (citable) accession number: P30560
Secondary accession number(s): Q5M8D1, Q62874, Q9QW18
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: June 13, 2006
Last modified: April 16, 2014
This is version 121 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries