Reviewed, UniProtKB/Swiss-Prot P30558 (PAR1_MOUSE)

Names and origin

Protein names

Recommended name:
    Proteinase-activated receptor 1
      Short name=PAR-1
Alternative name(s):
    Thrombin receptor

Gene names

Name:	F2r
Synonyms:	Cf2r, Par1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	430 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	High affinity receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Post-translational modification	A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Biological process	Blood coagulation
Cellular component	Cell membrane Membrane
Domain	Signal Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	blood coagulation Inferred from electronic annotation. Source: UniProtKB-KW death Inferred from mutant phenotype. Source: MGI homeostasis of number of cells within a tissue Inferred from mutant phenotype. Source: MGI inflammatory response Inferred from mutant phenotype. Source: MGI positive regulation of blood coagulation Inferred from mutant phenotype. Source: MGI regulation of interleukin-1 beta production Inferred from mutant phenotype. Source: MGI response to lipopolysaccharide Inferred from mutant phenotype. Source: MGI
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	G-protein coupled receptor activity Inferred from electronic annotation. Source: UniProtKB-KW thrombin receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Propeptide	22 – 41	20	Removed for receptor activation By similarity		PRO_0000012742
	Chain	42 – 430	389	Proteinase-activated receptor 1		PRO_0000012743
Regions
	Topological domain	42 – 107	66	Extracellular Potential
	Transmembrane	108 – 133	26	1 Potential
	Topological domain	134 – 142	9	Cytoplasmic Potential
	Transmembrane	143 – 162	20	2 Potential
	Topological domain	163 – 181	19	Extracellular Potential
	Transmembrane	182 – 203	22	3 Potential
	Topological domain	204 – 223	20	Cytoplasmic Potential
	Transmembrane	224 – 244	21	4 Potential
	Topological domain	245 – 273	29	Extracellular Potential
	Transmembrane	274 – 293	20	5 Potential
	Topological domain	294 – 316	23	Cytoplasmic Potential
	Transmembrane	317 – 339	23	6 Potential
	Topological domain	340 – 354	15	Extracellular Potential
	Transmembrane	355 – 379	25	7 Potential
	Topological domain	380 – 430	51	Cytoplasmic Potential
	Compositional bias	60 – 65	6	Asp/Glu-rich (acidic)
	Compositional bias	88 – 91	4	Poly-Pro
Sites
	Site	41 – 42	2	Cleavage; by thrombin By similarity
Amino acid modifications
	Glycosylation	67	1	N-linked (GlcNAc...) Potential
	Glycosylation	80	1	N-linked (GlcNAc...) Potential
	Glycosylation	255	1	N-linked (GlcNAc...) Potential
	Disulfide bond	180 ↔ 259		By similarity
Experimental info
	Sequence conflict	162	1	F → S in AAA40438. Ref.1
	Sequence conflict	189	1	G → Y in AAA40438. Ref.1
	Sequence conflict	223	1	R → G in AAA40438. Ref.1
	Sequence conflict	262	1	V → L in AAA40438. Ref.1
	Sequence conflict	365	1	S → T in AAA40438. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P30558-1 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 395FD64FAE52C9BF	FASTA	430	47,790
	10 20 30 40 50 60 MGPRRLLIVA LGLSLCGPLL SSRVPMSQPE SERTDATVNP RSFFLRNPSE NTFELVPLGD 70 80 90 100 110 120 EEEEEKNESV LLEGRAVYLN ISLPPHTPPP PFISEDASGY LTSPWLTLFM PSVYTIVFIV 130 140 150 160 170 180 SLPLNVLAIA VFVLRMKVKK PAVVYMLHLA MADVLFVSVL PFKISYYFSG TDWQFGSGMC 190 200 210 220 230 240 RFATAAFYGN MYASIMLMTV ISIDRFLAVV YPIQSLSWRT LGRANFTCVV IWVMAIMGVV 250 260 270 280 290 300 PLLLKEQTTR VPGLNITTCH DVLSENLMQG FYSYYFSAFS AIFFLVPLIV STVCYTSIIR 310 320 330 340 350 360 CLSSSAVANR SKKSRALFLS AAVFCIFIVC FGPTNVLLIV HYLFLSDSPG TEAAYFAYLL 370 380 390 400 410 420 CVCVSSVSCC IDPLIYYYAS SECQRHLYSI LCCKESSDPN SCNSTGQLMP SKMDTCSSHL 430 NNSIYKKLLA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of cDNA for the mouse thrombin receptor." Coughlin S.R. Submitted (OCT-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Tissue: Brain.
[2]	"Conserved structure and adjacent location of the thrombin receptor and protease-activated receptor 2 genes define a protease-activated receptor gene cluster." Kahn M.L., Ishii K., Kuo W.L., Piper M., Connolly A.J., Shi Y.P., Wu R., Lin C.C., Coughlin S.R. Mol. Med. 2:349-357(1996) [PubMed: 8784787] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Heart.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary gland.
[5]	"Structure and localization of the thrombin receptor gene on mouse chromosome 13." Xue J., Jenkins N.A., Gilbert D.J., Copeland N.G., Sadler J.E. Mamm. Genome 7:625-626(1996) [PubMed: 8678993] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-74. Strain: 129/Sv.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L03529 mRNA. Translation: AAA40438.1. U36757, U36756 Genomic DNA. Translation: AAB38308.1. AK085990 mRNA. Translation: BAC39587.1. AK159282 mRNA. Translation: BAE34960.1. AK160032 mRNA. Translation: BAE35575.1. BC031516 mRNA. Translation: AAH31516.1. U55076, U55075 Genomic DNA. Translation: AAB00198.1.
IPI	IPI00131268.
RefSeq	NP_034299.2.
UniGene	Mm.24816
3D structure databases
HSSP	HSSP built from PDB template 1DDD based on UniProtKB P34996.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P30558.
Genome annotation databases
Ensembl	ENSMUSG00000048376. Mus musculus. [Contig view]
GeneID	14062.
KEGG	mmu:14062.
NMPDR	fig|10090.3.peg.28270.
Organism-specific databases
MGI	MGI:101802. F2r.
Phylogenomic databases
HOGENOM	P30558.
HOVERGEN	P30558.
OMA	P30558. SVLPFKI.
Gene expression databases
ArrayExpress	P30558.
Bgee	P30558.
CleanEx	MM_F2R.
GermOnline	ENSMUSG00000048376. Mus musculus.
Family and domain databases
InterPro	IPR000276. 7TM_GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. IPR003912. Protea_act_rcpt. IPR000935. Thrmbn_rcpt. [Graphical view]
PANTHER	PTHR19264:SF221. Thrmbn_rcpt. 1 hit.
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR01428. PROTEASEAR. PR00908. THROMBINR.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	285032.
SOURCE	Search...

Entry information

Entry name

PAR1_MOUSE

Accession

Primary (citable) accession number: P30558
Secondary accession number(s): P97507, Q3TVP3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)