Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Proteinase-activated receptor 1

Gene

F2r

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

High affinity receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis.

GO - Molecular functioni

  • G-protein alpha-subunit binding Source: UniProtKB
  • G-protein beta-subunit binding Source: UniProtKB
  • G-protein coupled receptor activity Source: UniProtKB
  • heterotrimeric G-protein binding Source: UniProtKB
  • receptor binding Source: MGI
  • thrombin-activated receptor activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Blood coagulation, Hemostasis

Enzyme and pathway databases

ReactomeiR-MMU-140875. Common Pathway of Fibrin Clot Formation.
R-MMU-375276. Peptide ligand-binding receptors.
R-MMU-416476. G alpha (q) signalling events.
R-MMU-456926. Thrombin signalling through proteinase activated receptors (PARs).

Names & Taxonomyi

Protein namesi
Recommended name:
Proteinase-activated receptor 1
Short name:
PAR-1
Alternative name(s):
Thrombin receptor
Gene namesi
Name:F2r
Synonyms:Cf2r, Par1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:101802. F2r.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini42 – 107ExtracellularSequence analysisAdd BLAST66
Transmembranei108 – 133Helical; Name=1Sequence analysisAdd BLAST26
Topological domaini134 – 142CytoplasmicSequence analysis9
Transmembranei143 – 162Helical; Name=2Sequence analysisAdd BLAST20
Topological domaini163 – 181ExtracellularSequence analysisAdd BLAST19
Transmembranei182 – 203Helical; Name=3Sequence analysisAdd BLAST22
Topological domaini204 – 223CytoplasmicSequence analysisAdd BLAST20
Transmembranei224 – 244Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini245 – 273ExtracellularSequence analysisAdd BLAST29
Transmembranei274 – 293Helical; Name=5Sequence analysisAdd BLAST20
Topological domaini294 – 316CytoplasmicSequence analysisAdd BLAST23
Transmembranei317 – 339Helical; Name=6Sequence analysisAdd BLAST23
Topological domaini340 – 354ExtracellularSequence analysisAdd BLAST15
Transmembranei355 – 379Helical; Name=7Sequence analysisAdd BLAST25
Topological domaini380 – 430CytoplasmicSequence analysisAdd BLAST51

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21By similarityAdd BLAST21
PropeptideiPRO_000001274222 – 41Removed for receptor activationBy similarityAdd BLAST20
ChainiPRO_000001274342 – 430Proteinase-activated receptor 1Add BLAST389

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi67N-linked (GlcNAc...)1 Publication1
Glycosylationi80N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi180 ↔ 259PROSITE-ProRule annotation
Glycosylationi255N-linked (GlcNAc...)Sequence analysis1
Modified residuei423PhosphoserineBy similarity1

Post-translational modificationi

A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.
Phosphorylated in the C-terminal tail; probably mediating desensitization prior to the uncoupling and internalization of the receptor.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei41 – 42Cleavage; by thrombinBy similarity2

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP30558.
PaxDbiP30558.
PeptideAtlasiP30558.
PRIDEiP30558.

PTM databases

iPTMnetiP30558.
PhosphoSitePlusiP30558.

Expressioni

Gene expression databases

BgeeiENSMUSG00000048376.
CleanExiMM_F2R.
GenevisibleiP30558. MM.

Interactioni

GO - Molecular functioni

  • G-protein alpha-subunit binding Source: UniProtKB
  • G-protein beta-subunit binding Source: UniProtKB
  • heterotrimeric G-protein binding Source: UniProtKB
  • receptor binding Source: MGI

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000061754.

Structurei

3D structure databases

ProteinModelPortaliP30558.
SMRiP30558.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi60 – 65Asp/Glu-rich (acidic)6
Compositional biasi88 – 91Poly-Pro4

Domaini

The cleaved signal peptide may not be degraded and may function as an intracellular angiogenesis inhibitor peptide known as parstatin.By similarity

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IJCV. Eukaryota.
ENOG4110Q89. LUCA.
GeneTreeiENSGT00760000119001.
HOGENOMiHOG000232200.
HOVERGENiHBG108233.
InParanoidiP30558.
KOiK03914.
OMAiVHYLFLS.
OrthoDBiEOG091G0I94.
PhylomeDBiP30558.
TreeFamiTF330775.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR003912. Protea_act_rcpt.
IPR000935. Thrmbn_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01428. PROTEASEAR.
PR00908. THROMBINR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30558-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGPRRLLIVA LGLSLCGPLL SSRVPMSQPE SERTDATVNP RSFFLRNPSE
60 70 80 90 100
NTFELVPLGD EEEEEKNESV LLEGRAVYLN ISLPPHTPPP PFISEDASGY
110 120 130 140 150
LTSPWLTLFM PSVYTIVFIV SLPLNVLAIA VFVLRMKVKK PAVVYMLHLA
160 170 180 190 200
MADVLFVSVL PFKISYYFSG TDWQFGSGMC RFATAAFYGN MYASIMLMTV
210 220 230 240 250
ISIDRFLAVV YPIQSLSWRT LGRANFTCVV IWVMAIMGVV PLLLKEQTTR
260 270 280 290 300
VPGLNITTCH DVLSENLMQG FYSYYFSAFS AIFFLVPLIV STVCYTSIIR
310 320 330 340 350
CLSSSAVANR SKKSRALFLS AAVFCIFIVC FGPTNVLLIV HYLFLSDSPG
360 370 380 390 400
TEAAYFAYLL CVCVSSVSCC IDPLIYYYAS SECQRHLYSI LCCKESSDPN
410 420 430
SCNSTGQLMP SKMDTCSSHL NNSIYKKLLA
Length:430
Mass (Da):47,790
Last modified:November 1, 1997 - v2
Checksum:i395FD64FAE52C9BF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti162F → S in AAA40438 (Ref. 1) Curated1
Sequence conflicti189G → Y in AAA40438 (Ref. 1) Curated1
Sequence conflicti223R → G in AAA40438 (Ref. 1) Curated1
Sequence conflicti262V → L in AAA40438 (Ref. 1) Curated1
Sequence conflicti365S → T in AAA40438 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L03529 mRNA. Translation: AAA40438.1.
U36757, U36756 Genomic DNA. Translation: AAB38308.1.
AK085990 mRNA. Translation: BAC39587.1.
AK159282 mRNA. Translation: BAE34960.1.
AK160032 mRNA. Translation: BAE35575.1.
BC031516 mRNA. Translation: AAH31516.1.
AH003565 Genomic DNA. Translation: AAB00198.1.
CCDSiCCDS26701.1.
RefSeqiNP_034299.2. NM_010169.3.
UniGeneiMm.24816.

Genome annotation databases

EnsembliENSMUST00000059193; ENSMUSP00000061754; ENSMUSG00000048376.
GeneIDi14062.
KEGGimmu:14062.
UCSCiuc007rmn.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L03529 mRNA. Translation: AAA40438.1.
U36757, U36756 Genomic DNA. Translation: AAB38308.1.
AK085990 mRNA. Translation: BAC39587.1.
AK159282 mRNA. Translation: BAE34960.1.
AK160032 mRNA. Translation: BAE35575.1.
BC031516 mRNA. Translation: AAH31516.1.
AH003565 Genomic DNA. Translation: AAB00198.1.
CCDSiCCDS26701.1.
RefSeqiNP_034299.2. NM_010169.3.
UniGeneiMm.24816.

3D structure databases

ProteinModelPortaliP30558.
SMRiP30558.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000061754.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiP30558.
PhosphoSitePlusiP30558.

Proteomic databases

MaxQBiP30558.
PaxDbiP30558.
PeptideAtlasiP30558.
PRIDEiP30558.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000059193; ENSMUSP00000061754; ENSMUSG00000048376.
GeneIDi14062.
KEGGimmu:14062.
UCSCiuc007rmn.1. mouse.

Organism-specific databases

CTDi2149.
MGIiMGI:101802. F2r.

Phylogenomic databases

eggNOGiENOG410IJCV. Eukaryota.
ENOG4110Q89. LUCA.
GeneTreeiENSGT00760000119001.
HOGENOMiHOG000232200.
HOVERGENiHBG108233.
InParanoidiP30558.
KOiK03914.
OMAiVHYLFLS.
OrthoDBiEOG091G0I94.
PhylomeDBiP30558.
TreeFamiTF330775.

Enzyme and pathway databases

ReactomeiR-MMU-140875. Common Pathway of Fibrin Clot Formation.
R-MMU-375276. Peptide ligand-binding receptors.
R-MMU-416476. G alpha (q) signalling events.
R-MMU-456926. Thrombin signalling through proteinase activated receptors (PARs).

Miscellaneous databases

PROiP30558.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000048376.
CleanExiMM_F2R.
GenevisibleiP30558. MM.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR003912. Protea_act_rcpt.
IPR000935. Thrmbn_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01428. PROTEASEAR.
PR00908. THROMBINR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPAR1_MOUSE
AccessioniPrimary (citable) accession number: P30558
Secondary accession number(s): P97507, Q3TVP3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: November 1, 1997
Last modified: November 30, 2016
This is version 152 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.