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P30558

- PAR1_MOUSE

UniProt

P30558 - PAR1_MOUSE

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Protein

Proteinase-activated receptor 1

Gene

F2r

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

High affinity receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei41 – 422Cleavage; by thrombinBy similarity

GO - Molecular functioni

  1. G-protein alpha-subunit binding Source: UniProtKB
  2. G-protein beta-subunit binding Source: UniProtKB
  3. G-protein coupled receptor activity Source: UniProtKB
  4. heterotrimeric G-protein binding Source: UniProtKB
  5. thrombin receptor activity Source: UniProtKB

GO - Biological processi

  1. activation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
  2. activation of MAPKK activity Source: UniProtKB
  3. connective tissue replacement involved in inflammatory response wound healing Source: UniProtKB
  4. death Source: MGI
  5. establishment of synaptic specificity at neuromuscular junction Source: UniProtKB
  6. homeostasis of number of cells within a tissue Source: MGI
  7. inflammatory response Source: MGI
  8. negative regulation of cell proliferation Source: UniProtKB
  9. negative regulation of glomerular filtration Source: UniProtKB
  10. negative regulation of neuron apoptotic process Source: UniProtKB
  11. negative regulation of renin secretion into blood stream Source: UniProtKB
  12. platelet activation Source: UniProtKB
  13. positive regulation of blood coagulation Source: MGI
  14. positive regulation of calcium ion transport Source: UniProtKB
  15. positive regulation of cell migration Source: UniProtKB
  16. positive regulation of cell proliferation Source: UniProtKB
  17. positive regulation of collagen biosynthetic process Source: UniProtKB
  18. positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
  19. positive regulation of cytosolic calcium ion concentration Source: UniProtKB
  20. positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway Source: UniProtKB
  21. positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
  22. positive regulation of I-kappaB kinase/NF-kappaB signaling Source: UniProtKB
  23. positive regulation of interleukin-6 secretion Source: UniProtKB
  24. positive regulation of interleukin-8 secretion Source: UniProtKB
  25. positive regulation of JAK-STAT cascade Source: UniProtKB
  26. positive regulation of MAPK cascade Source: UniProtKB
  27. positive regulation of phosphatidylinositol 3-kinase signaling Source: UniProtKB
  28. positive regulation of release of sequestered calcium ion into cytosol Source: UniProtKB
  29. positive regulation of Rho protein signal transduction Source: UniProtKB
  30. positive regulation of smooth muscle contraction Source: UniProtKB
  31. positive regulation of transcription, DNA-templated Source: UniProtKB
  32. positive regulation of vasoconstriction Source: UniProtKB
  33. protein kinase C-activating G-protein coupled receptor signaling pathway Source: UniProtKB
  34. regulation of interleukin-1 beta production Source: MGI
  35. regulation of sensory perception of pain Source: UniProtKB
  36. release of sequestered calcium ion into cytosol Source: UniProtKB
  37. response to lipopolysaccharide Source: MGI
  38. response to wounding Source: UniProtKB
  39. STAT protein import into nucleus Source: UniProtKB
  40. thrombin receptor signaling pathway Source: MGI
  41. tyrosine phosphorylation of STAT protein Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Blood coagulation, Hemostasis

Enzyme and pathway databases

ReactomeiREACT_207651. G alpha (q) signalling events.
REACT_220322. Thrombin signalling through proteinase activated receptors (PARs).
REACT_235286. Peptide ligand-binding receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteinase-activated receptor 1
Short name:
PAR-1
Alternative name(s):
Thrombin receptor
Gene namesi
Name:F2r
Synonyms:Cf2r, Par1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 13

Organism-specific databases

MGIiMGI:101802. F2r.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini42 – 10766ExtracellularSequence AnalysisAdd
BLAST
Transmembranei108 – 13326Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini134 – 1429CytoplasmicSequence Analysis
Transmembranei143 – 16220Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini163 – 18119ExtracellularSequence AnalysisAdd
BLAST
Transmembranei182 – 20322Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini204 – 22320CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei224 – 24421Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini245 – 27329ExtracellularSequence AnalysisAdd
BLAST
Transmembranei274 – 29320Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini294 – 31623CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei317 – 33923Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini340 – 35415ExtracellularSequence AnalysisAdd
BLAST
Transmembranei355 – 37925Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini380 – 43051CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. caveola Source: UniProtKB
  2. integral component of membrane Source: UniProtKB-KW
  3. neuromuscular junction Source: UniProtKB
  4. plasma membrane Source: UniProtKB
  5. platelet dense tubular network Source: UniProtKB
  6. postsynaptic membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121By similarityAdd
BLAST
Propeptidei22 – 4120Removed for receptor activationBy similarityPRO_0000012742Add
BLAST
Chaini42 – 430389Proteinase-activated receptor 1PRO_0000012743Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi67 – 671N-linked (GlcNAc...)1 Publication
Glycosylationi80 – 801N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi180 ↔ 259PROSITE-ProRule annotation
Glycosylationi255 – 2551N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.
Phosphorylated in the C-terminal tail; probably mediating desensitization prior to the uncoupling and internalization of the receptor.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP30558.
PRIDEiP30558.

PTM databases

PhosphoSiteiP30558.

Expressioni

Gene expression databases

BgeeiP30558.
CleanExiMM_F2R.
GenevestigatoriP30558.

Structurei

3D structure databases

ProteinModelPortaliP30558.
SMRiP30558. Positions 73-393.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi60 – 656Asp/Glu-rich (acidic)
Compositional biasi88 – 914Poly-Pro

Domaini

The cleaved signal peptide may not be degraded and may function as an intracellular angiogenesis inhibitor peptide known as parstatin.By similarity

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG150457.
GeneTreeiENSGT00760000119001.
HOGENOMiHOG000232200.
HOVERGENiHBG108233.
InParanoidiP30558.
KOiK03914.
OMAiVHYLFLS.
OrthoDBiEOG7V49ZD.
PhylomeDBiP30558.
TreeFamiTF330775.

Family and domain databases

Gene3Di1.20.1070.10. 1 hit.
InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR003912. Protea_act_rcpt.
IPR000935. Thrmbn_rcpt.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01428. PROTEASEAR.
PR00908. THROMBINR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30558-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGPRRLLIVA LGLSLCGPLL SSRVPMSQPE SERTDATVNP RSFFLRNPSE
60 70 80 90 100
NTFELVPLGD EEEEEKNESV LLEGRAVYLN ISLPPHTPPP PFISEDASGY
110 120 130 140 150
LTSPWLTLFM PSVYTIVFIV SLPLNVLAIA VFVLRMKVKK PAVVYMLHLA
160 170 180 190 200
MADVLFVSVL PFKISYYFSG TDWQFGSGMC RFATAAFYGN MYASIMLMTV
210 220 230 240 250
ISIDRFLAVV YPIQSLSWRT LGRANFTCVV IWVMAIMGVV PLLLKEQTTR
260 270 280 290 300
VPGLNITTCH DVLSENLMQG FYSYYFSAFS AIFFLVPLIV STVCYTSIIR
310 320 330 340 350
CLSSSAVANR SKKSRALFLS AAVFCIFIVC FGPTNVLLIV HYLFLSDSPG
360 370 380 390 400
TEAAYFAYLL CVCVSSVSCC IDPLIYYYAS SECQRHLYSI LCCKESSDPN
410 420 430
SCNSTGQLMP SKMDTCSSHL NNSIYKKLLA
Length:430
Mass (Da):47,790
Last modified:November 1, 1997 - v2
Checksum:i395FD64FAE52C9BF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti162 – 1621F → S in AAA40438. 1 PublicationCurated
Sequence conflicti189 – 1891G → Y in AAA40438. 1 PublicationCurated
Sequence conflicti223 – 2231R → G in AAA40438. 1 PublicationCurated
Sequence conflicti262 – 2621V → L in AAA40438. 1 PublicationCurated
Sequence conflicti365 – 3651S → T in AAA40438. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L03529 mRNA. Translation: AAA40438.1.
U36757, U36756 Genomic DNA. Translation: AAB38308.1.
AK085990 mRNA. Translation: BAC39587.1.
AK159282 mRNA. Translation: BAE34960.1.
AK160032 mRNA. Translation: BAE35575.1.
BC031516 mRNA. Translation: AAH31516.1.
AH003565 Genomic DNA. Translation: AAB00198.1.
CCDSiCCDS26701.1.
RefSeqiNP_034299.2. NM_010169.3.
UniGeneiMm.24816.

Genome annotation databases

EnsembliENSMUST00000059193; ENSMUSP00000061754; ENSMUSG00000048376.
GeneIDi14062.
KEGGimmu:14062.
UCSCiuc007rmn.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L03529 mRNA. Translation: AAA40438.1 .
U36757 , U36756 Genomic DNA. Translation: AAB38308.1 .
AK085990 mRNA. Translation: BAC39587.1 .
AK159282 mRNA. Translation: BAE34960.1 .
AK160032 mRNA. Translation: BAE35575.1 .
BC031516 mRNA. Translation: AAH31516.1 .
AH003565 Genomic DNA. Translation: AAB00198.1 .
CCDSi CCDS26701.1.
RefSeqi NP_034299.2. NM_010169.3.
UniGenei Mm.24816.

3D structure databases

ProteinModelPortali P30558.
SMRi P30558. Positions 73-393.
ModBasei Search...
MobiDBi Search...

Chemistry

GuidetoPHARMACOLOGYi 347.

Protein family/group databases

GPCRDBi Search...

PTM databases

PhosphoSitei P30558.

Proteomic databases

MaxQBi P30558.
PRIDEi P30558.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000059193 ; ENSMUSP00000061754 ; ENSMUSG00000048376 .
GeneIDi 14062.
KEGGi mmu:14062.
UCSCi uc007rmn.1. mouse.

Organism-specific databases

CTDi 2149.
MGIi MGI:101802. F2r.

Phylogenomic databases

eggNOGi NOG150457.
GeneTreei ENSGT00760000119001.
HOGENOMi HOG000232200.
HOVERGENi HBG108233.
InParanoidi P30558.
KOi K03914.
OMAi VHYLFLS.
OrthoDBi EOG7V49ZD.
PhylomeDBi P30558.
TreeFami TF330775.

Enzyme and pathway databases

Reactomei REACT_207651. G alpha (q) signalling events.
REACT_220322. Thrombin signalling through proteinase activated receptors (PARs).
REACT_235286. Peptide ligand-binding receptors.

Miscellaneous databases

NextBioi 285032.
PROi P30558.
SOURCEi Search...

Gene expression databases

Bgeei P30558.
CleanExi MM_F2R.
Genevestigatori P30558.

Family and domain databases

Gene3Di 1.20.1070.10. 1 hit.
InterProi IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR003912. Protea_act_rcpt.
IPR000935. Thrmbn_rcpt.
[Graphical view ]
Pfami PF00001. 7tm_1. 1 hit.
[Graphical view ]
PRINTSi PR00237. GPCRRHODOPSN.
PR01428. PROTEASEAR.
PR00908. THROMBINR.
PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of cDNA for the mouse thrombin receptor."
    Coughlin S.R.
    Submitted (OCT-1992) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "Conserved structure and adjacent location of the thrombin receptor and protease-activated receptor 2 genes define a protease-activated receptor gene cluster."
    Kahn M.L., Ishii K., Kuo W.L., Piper M., Connolly A.J., Shi Y.P., Wu R., Lin C.C., Coughlin S.R.
    Mol. Med. 2:349-357(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Heart.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary gland.
  5. "Structure and localization of the thrombin receptor gene on mouse chromosome 13."
    Xue J., Jenkins N.A., Gilbert D.J., Copeland N.G., Sadler J.E.
    Mamm. Genome 7:625-626(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-74.
    Strain: 129/Sv.
  6. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-67.

Entry informationi

Entry nameiPAR1_MOUSE
AccessioniPrimary (citable) accession number: P30558
Secondary accession number(s): P97507, Q3TVP3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: November 1, 1997
Last modified: November 26, 2014
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3