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Protein

Prostaglandin E2 receptor EP3 subtype

Gene

Ptger3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for prostaglandin E2 (PGE2); the EP3 receptor may be involved in inhibition of gastric acid secretion, modulation of neurotransmitter release in central and peripheral neurons, inhibition of sodium and water reabsorption in kidney tubulus and contraction in uterine smooth muscle. The activity of this receptor can couple to both the inhibition of adenylate cyclase mediated by G(i) proteins, and to an elevation of intracellular calcium. The various forms can interact with different second messenger systems.

GO - Molecular functioni

  • prostaglandin E receptor activity Source: MGI

GO - Biological processi

  • adenylate cyclase-modulating G-protein coupled receptor signaling pathway Source: MGI
  • bicarbonate transport Source: MGI
  • fever generation Source: MGI
  • intestine smooth muscle contraction Source: GO_Central
  • negative regulation of gastric acid secretion Source: GO_Central
  • phospholipase C-activating G-protein coupled receptor signaling pathway Source: MGI
  • positive regulation of cytosolic calcium ion concentration Source: MGI
  • positive regulation of fever generation Source: BHF-UCL
  • positive regulation of urine volume Source: MGI
  • response to lipopolysaccharide Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
Prostaglandin E2 receptor EP3 subtype
Short name:
PGE receptor EP3 subtype
Short name:
PGE2 receptor EP3 subtype
Alternative name(s):
Prostanoid EP3 receptor
Gene namesi
Name:Ptger3
Synonyms:Ptgerep3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:97795. Ptger3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 30ExtracellularSequence analysisAdd BLAST30
Transmembranei31 – 55Helical; Name=1Sequence analysisAdd BLAST25
Topological domaini56 – 68CytoplasmicSequence analysisAdd BLAST13
Transmembranei69 – 89Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini90 – 108ExtracellularSequence analysisAdd BLAST19
Transmembranei109 – 130Helical; Name=3Sequence analysisAdd BLAST22
Topological domaini131 – 151CytoplasmicSequence analysisAdd BLAST21
Transmembranei152 – 173Helical; Name=4Sequence analysisAdd BLAST22
Topological domaini174 – 203ExtracellularSequence analysisAdd BLAST30
Transmembranei204 – 229Helical; Name=5Sequence analysisAdd BLAST26
Topological domaini230 – 259CytoplasmicSequence analysisAdd BLAST30
Transmembranei260 – 283Helical; Name=6Sequence analysisAdd BLAST24
Topological domaini284 – 303ExtracellularSequence analysisAdd BLAST20
Transmembranei304 – 325Helical; Name=7Sequence analysisAdd BLAST22
Topological domaini326 – 365CytoplasmicSequence analysisAdd BLAST40

GO - Cellular componenti

  • integral component of plasma membrane Source: GO_Central
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4336.
GuidetoPHARMACOLOGYi342.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000700591 – 365Prostaglandin E2 receptor EP3 subtypeAdd BLAST365

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi16N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi107 ↔ 184PROSITE-ProRule annotation
Glycosylationi193N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Ligand binding is affected by cAMP-dependent phosphorylation in brain membranes.

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP30557.
PRIDEiP30557.

PTM databases

iPTMnetiP30557.
PhosphoSitePlusiP30557.

Expressioni

Tissue specificityi

Kidney, uterus, and mastocytoma cells, and in a lesser amount in brain, thymus, lung, heart, stomach and spleen.

Gene expression databases

CleanExiMM_PTGER3.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000043302.

Chemistry databases

BindingDBiP30557.

Structurei

3D structure databases

ProteinModelPortaliP30557.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
HOGENOMiHOG000015238.
HOVERGENiHBG052720.
InParanoidiP30557.
PhylomeDBiP30557.

Family and domain databases

InterProiIPR000154. EP3_rcpt_3.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR008365. Prostanoid_rcpt.
IPR001244. Prostglndn_DP_rcpt.
IPR000265. Prostglndn_EP3_rcpt.
[Graphical view]
PANTHERiPTHR11866. PTHR11866. 1 hit.
PTHR11866:SF10. PTHR11866:SF10. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00428. PROSTAGLNDNR.
PR01788. PROSTANOIDR.
PR00585. PRSTNOIDE33R.
PR00582. PRSTNOIDEP3R.
PROSITEiPS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist. Isoforms have identical ligand binding properties but different coupling properties with G proteins: isoform Alpha and isoform Beta couple to G(i) proteins, whereas isoform Gamma couples to multiple G proteins, G(i) and G(s). Isoform Alpha shows high agonist-independent constitutive inhibition of adenylate cyclase, while isoform Beta has no agonist independent inhibition.
Isoform Alpha (identifier: P30557-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASMWAPEHS AEAHSNLSST TDDCGSVSVA FPITMMVTGF VGNALAMLLV
60 70 80 90 100
SRSYRRRESK RKKSFLLCIG WLALTDLVGQ LLTSPVVILV YLSQRRWEQL
110 120 130 140 150
DPSGRLCTFF GLTMTVFGLS SLLVASAMAV ERALAIRAPH WYASHMKTRA
160 170 180 190 200
TPVLLGVWLS VLAFALLPVL GVGRYSVQWP GTWCFISTGP AGNETDPARE
210 220 230 240 250
PGSVAFASAF ACLGLLALVV TFACNLATIK ALVSRCRAKA AVSQSSAQWG
260 270 280 290 300
RITTETAIQL MGIMCVLSVC WSPLLIMMLK MIFNQMSVEQ CKTQMGKEKE
310 320 330 340 350
CNSFLIAVRL ASLNQILDPW VYLLLRKILL RKFCQIRDHT NYASSSTSLP
360
CPGSSALMWS DQLER
Length:365
Mass (Da):40,077
Last modified:April 1, 1993 - v1
Checksum:i1FEBEBB30C5EA67E
GO
Isoform Beta (identifier: P30557-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     336-365: IRDHTNYASSSTSLPCPGSSALMWSDQLER → MMNNLKWTFIAVPVSLGLRISSPREG

Show »
Length:361
Mass (Da):39,684
Checksum:iFB1B5310C28320CE
GO
Isoform Gamma (identifier: P30557-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     336-365: IRDHTNYASSSTSLPCPGSSALMWSDQLER → VANAVSSCSSDGQKGQAISLSNEVVQPGP

Show »
Length:364
Mass (Da):39,597
Checksum:iF14BDBA15F055FD3
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_001940336 – 365IRDHT…DQLER → MMNNLKWTFIAVPVSLGLRI SSPREG in isoform Beta. 1 PublicationAdd BLAST30
Alternative sequenceiVSP_001941336 – 365IRDHT…DQLER → VANAVSSCSSDGQKGQAISL SNEVVQPGP in isoform Gamma. 1 PublicationAdd BLAST30

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10204 mRNA. Translation: BAA01051.1.
D13321 mRNA. Translation: BAA02578.1.
D17406 mRNA. Translation: BAA04229.1.
PIRiA42414.
A45211.
S65009.
UniGeneiMm.30424.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D10204 mRNA. Translation: BAA01051.1.
D13321 mRNA. Translation: BAA02578.1.
D17406 mRNA. Translation: BAA04229.1.
PIRiA42414.
A45211.
S65009.
UniGeneiMm.30424.

3D structure databases

ProteinModelPortaliP30557.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000043302.

Chemistry databases

BindingDBiP30557.
ChEMBLiCHEMBL4336.
GuidetoPHARMACOLOGYi342.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiP30557.
PhosphoSitePlusiP30557.

Proteomic databases

PaxDbiP30557.
PRIDEiP30557.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:97795. Ptger3.

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
HOGENOMiHOG000015238.
HOVERGENiHBG052720.
InParanoidiP30557.
PhylomeDBiP30557.

Miscellaneous databases

PROiP30557.
SOURCEiSearch...

Gene expression databases

CleanExiMM_PTGER3.

Family and domain databases

InterProiIPR000154. EP3_rcpt_3.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR008365. Prostanoid_rcpt.
IPR001244. Prostglndn_DP_rcpt.
IPR000265. Prostglndn_EP3_rcpt.
[Graphical view]
PANTHERiPTHR11866. PTHR11866. 1 hit.
PTHR11866:SF10. PTHR11866:SF10. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00428. PROSTAGLNDNR.
PR01788. PROSTANOIDR.
PR00585. PRSTNOIDE33R.
PR00582. PRSTNOIDEP3R.
PROSITEiPS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPE2R3_MOUSE
AccessioniPrimary (citable) accession number: P30557
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: November 2, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.