P30556 (AGTR1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-1 angiotensin II receptor

Alternative name(s):
AT1AR
AT1BR
Angiotensin II type-1 receptor
Short name=AT1

Gene names

Name:	AGTR1
Synonyms:	AGTR1A, AGTR1B, AT2R1, AT2R1B

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for angiotensin II. Mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Subunit structure	Interacts with MAS1 Probable. Interacts with ARRB1 By similarity. Ref.11
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Liver, lung, adrenal and adrenocortical adenomas.
Post-translational modification	C-terminal Ser or Thr residues may be phosphorylated.
Involvement in disease	Renal tubular dysgenesis (RTD) [MIM:267430]: Autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.12
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Polymorphism
Disease	Disease mutation
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Rho protein signal transduction Inferred from direct assay PubMed 15611106. Source: UniProtKB calcium-mediated signaling Inferred from direct assay Ref.3. Source: BHF-UCL cell chemotaxis Inferred from direct assay PubMed 15635042. Source: UniProtKB elevation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway Inferred from direct assay Ref.3. Source: BHF-UCL kidney development Inferred from mutant phenotype Ref.12. Source: BHF-UCL low-density lipoprotein particle remodeling Non-traceable author statement PubMed 17069818. Source: BHF-UCL positive regulation of NAD(P)H oxidase activity Traceable author statement PubMed 17159080. Source: BHF-UCL positive regulation of cellular protein metabolic process Inferred from mutant phenotype PubMed 18971559. Source: BHF-UCL positive regulation of cholesterol esterification Inferred from mutant phenotype PubMed 18971559. Source: BHF-UCL positive regulation of inflammatory response Traceable author statement PubMed 17159080. Source: BHF-UCL positive regulation of macrophage derived foam cell differentiation Inferred by curator PubMed 18971559. Source: BHF-UCL positive regulation of phospholipase A2 activity Inferred from mutant phenotype PubMed 17069818. Source: BHF-UCL positive regulation of reactive oxygen species metabolic process Traceable author statement PubMed 17159080. Source: BHF-UCL regulation of blood vessel size by renin-angiotensin Inferred by curator PubMed 10993080. Source: BHF-UCL regulation of cell growth Non-traceable author statement PubMed 17159080. Source: BHF-UCL regulation of cell proliferation Non-traceable author statement PubMed 17159080. Source: BHF-UCL regulation of renal sodium excretion Non-traceable author statement PubMed 17159080. Source: BHF-UCL regulation of vasoconstriction Inferred from direct assay PubMed 15117835. Source: BHF-UCL regulation of vasodilation Inferred by curator Ref.1. Source: BHF-UCL renin-angiotensin regulation of aldosterone production Non-traceable author statement PubMed 17159080. Source: BHF-UCL
Cellular_component	integral to plasma membrane Inferred by curator Ref.1. Source: BHF-UCL
Molecular_function	acetyltransferase activator activity Inferred from mutant phenotype PubMed 18971559. Source: BHF-UCL angiotensin type I receptor activity Inferred from direct assay PubMed 10993080. Source: BHF-UCL angiotensin type II receptor activity Inferred from direct assay PubMed 15611106. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 359	359	Type-1 angiotensin II receptor		PRO_0000069153
Regions
	Topological domain	1 – 27	27	Extracellular Potential
	Transmembrane	28 – 52	25	Helical; Name=1; Potential
	Topological domain	53 – 64	12	Cytoplasmic Potential
	Transmembrane	65 – 87	23	Helical; Name=2; Potential
	Topological domain	88 – 102	15	Extracellular Potential
	Transmembrane	103 – 124	22	Helical; Name=3; Potential
	Topological domain	125 – 142	18	Cytoplasmic Potential
	Transmembrane	143 – 162	20	Helical; Name=4; Potential
	Topological domain	163 – 192	30	Extracellular Potential
	Transmembrane	193 – 214	22	Helical; Name=5; Potential
	Topological domain	215 – 240	26	Cytoplasmic Potential
	Transmembrane	241 – 262	22	Helical; Name=6; Potential
	Topological domain	263 – 275	13	Extracellular Potential
	Transmembrane	276 – 296	21	Helical; Name=7; Potential
	Topological domain	297 – 359	63	Cytoplasmic Potential
Amino acid modifications
	Lipidation	355	1	S-palmitoyl cysteine Potential
	Glycosylation	4	1	N-linked (GlcNAc...) Potential
	Glycosylation	176	1	N-linked (GlcNAc...) Potential
	Glycosylation	188	1	N-linked (GlcNAc...) Potential
	Disulfide bond	101 ↔ 180		By similarity
Natural variations
	Natural variant	48	1	L → V. Corresponds to variant rs2011425 [ dbSNP | Ensembl ].		VAR_011846
	Natural variant	163	1	A → T. Corresponds to variant rs12721226 [ dbSNP | Ensembl ].		VAR_029206
	Natural variant	244	1	A → S. Corresponds to variant rs12721225 [ dbSNP | Ensembl ].		VAR_029207
	Natural variant	282	1	T → M in RTD. Ref.12		VAR_035086
	Natural variant	289	1	C → W. Ref.6 Corresponds to variant rs1064533 [ dbSNP | Ensembl ].		VAR_011847
	Natural variant	336	1	T → P. Corresponds to variant rs1801021 [ dbSNP | Ensembl ].		VAR_011848
Experimental info
	Sequence conflict	187	1	Q → R in BAA02968. Ref.6
	Sequence conflict	204 – 205	2	FL → SC in BAA02968. Ref.6
	Sequence conflict	232	1	K → N in BAA02968. Ref.6
	Sequence conflict	240	1	K → R in BAA02968. Ref.6
	Sequence conflict	268	1	L → Q in BAA02968. Ref.6
	Sequence conflict	311 – 313	3	RYF → KDI in BAA02968. Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P30556 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 35FC856F53E911A6	FASTA	359	41,061
	10 20 30 40 50 60 MILNSSTEDG IKRIQDDCPK AGRHNYIFVM IPTLYSIIFV VGIFGNSLVV IVIYFYMKLK 70 80 90 100 110 120 TVASVFLLNL ALADLCFLLT LPLWAVYTAM EYRWPFGNYL CKIASASVSF NLYASVFLLT 130 140 150 160 170 180 CLSIDRYLAI VHPMKSRLRR TMLVAKVTCI IIWLLAGLAS LPAIIHRNVF FIENTNITVC 190 200 210 220 230 240 AFHYESQNST LPIGLGLTKN ILGFLFPFLI ILTSYTLIWK ALKKAYEIQK NKPRNDDIFK 250 260 270 280 290 300 IIMAIVLFFF FSWIPHQIFT FLDVLIQLGI IRDCRIADIV DTAMPITICI AYFNNCLNPL 310 320 330 340 350 FYGFLGKKFK RYFLQLLKYI PPKAKSHSNL STKMSTLSYR PSDNVSSSTK KPAPCFEVE

References

[1]	"Cloning, expression, and characterization of a gene encoding the human angiotensin II type 1A receptor." Mauzy C.A., Hwang O., Egloff A.M., Wu L.H., Chung F.-Z. Biochem. Biophys. Res. Commun. 186:277-284(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Molecular cloning and sequencing of the gene encoding human angiotensin II type 1 receptor." Furuta H., Guo D.F., Inagami T. Biochem. Biophys. Res. Commun. 183:8-13(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Lymphocyte.
[3]	"Cloning and characterization of a human angiotensin II type 1 receptor." Bergsma D.J., Ellis C., Kumar C., Nuthalaganti P., Kersten H., Elshourbagy N.A., Griffin E., Stadel J.M., Aiyar N. Biochem. Biophys. Res. Commun. 183:989-995(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[4]	"Molecular cloning, sequence analysis and expression of a cDNA encoding human type-1 angiotensin II receptor." Takayanagi R., Ohnaka K., Sakai Y., Nakao R., Yanase T., Haji M., Inagami T., Furuta H., Gou D.F., Nakamuta M., Nawata H. Biochem. Biophys. Res. Commun. 183:910-916(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[5]	"Genetic analysis of the human type-1 angiotensin II receptor." Curnow K.M., Pascoe L., White P.C. Mol. Endocrinol. 6:1113-1118(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[6]	"Novel subtype of human angiotensin II type 1 receptor: cDNA cloning and expression." Konishi H., Kuroda S., Inada Y., Fujisawa Y. Biochem. Biophys. Res. Commun. 199:467-474(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT TRP-289. Tissue: Placenta.
[7]	"Type 1 angiotensin II receptors of adrenal tumors." Nawata H., Takayanagi R., Ohnaka K., Sakai Y., Imasaki K., Yanase T., Ikuyama S., Tanaka S., Ohe K. Steroids 60:28-34(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[8]	"Cloning and sequencing of a human cDNA encoding the angiotensin II receptor type 1." Ostermann E., Castanon M.J. Submitted (JUL-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Placenta.
[9]	"Rapid identification of polymorphisms in genomic DNA: a high density SNP map of the type I angiotensin II receptor gene locus on chromosome 3q." Antonellis A., Rogus J.J., Pezzolesi M.G., Makita Y., Nam M., Doria A., Warram J.H., Krolewski A.S. Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[10]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Kopatz S.A., Aronstam R.S., Sharma S.V. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[11]	"G-protein-coupled receptor Mas is a physiological antagonist of the angiotensin II type 1 receptor." Kostenis E., Milligan G., Christopoulos A., Sanchez-Ferrer C.F., Heringer-Walther S., Sexton P.M., Gembardt F., Kellett E., Martini L., Vanderheyden P., Schultheiss H.P., Walther T. Circulation 111:1806-1813(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH MAS1.
[12]	"Mutations in genes in the renin-angiotensin system are associated with autosomal recessive renal tubular dysgenesis." Gribouval O., Gonzales M., Neuhaus T., Aziza J., Bieth E., Laurent N., Bouton J.M., Feuillet F., Makni S., Ben Amar H., Laube G., Delezoide A.-L., Bouvier R., Dijoud F., Ollagnon-Roman E., Roume J., Joubert M., Antignac C., Gubler M.-C. Nat. Genet. 37:964-968(2005) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT RTD MET-282.
+	Additional computationally mapped references.

Web resources

GeneReviews

SHMPD

The Singapore human mutation and polymorphism database

Wikipedia

Angiotensin receptor entry

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M91464 Genomic DNA. Translation: AAA35569.1.
Z11162 Genomic DNA. Translation: CAA77513.1.
M87290 mRNA. Translation: AAA35535.1.
S77410 mRNA. Translation: AAB34644.1.
M93394 mRNA. Translation: AAA58370.1.
D13814 mRNA. Translation: BAA02968.1.
X65699 mRNA. Translation: CAA46621.1.
AF245699 Genomic DNA. Translation: AAF70464.1.
AY221090 Genomic DNA. Translation: AAO65968.1.

IPI

IPI00797565.

PIR

I39418.
JC1104.

RefSeq

NP_000676.1. NM_000685.4.
NP_033611.1. NM_009585.3.

UniGene

Hs.477887.
Hs.740357.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ZV0	model	-	B	1-359	[»]

ProteinModelPortal

P30556.

ModBase

Search...

Protein-protein interaction databases

MINT

MINT-107041.

STRING

9606.ENSP00000273430.

Protein family/group databases

GPCRDB

Search...

PTM databases

PhosphoSite

P30556.

Polymorphism databases

DMDM

231519.

Proteomic databases

PaxDb

P30556.

PRIDE

P30556.

Protocols and materials databases

DNASU

185.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000349243; ENSP00000273430; ENSG00000144891.
ENST00000402260; ENSP00000385641; ENSG00000144891.
ENST00000404754; ENSP00000385612; ENSG00000144891.
ENST00000418473; ENSP00000398832; ENSG00000144891.
ENST00000461609; ENSP00000418851; ENSG00000144891.
ENST00000474935; ENSP00000418084; ENSG00000144891.
ENST00000475347; ENSP00000419783; ENSG00000144891.
ENST00000497524; ENSP00000419422; ENSG00000144891.
ENST00000542281; ENSP00000443186; ENSG00000144891.

GeneID

185.

KEGG

hsa:185.

UCSC

uc003ewg.3. human.

Organism-specific databases

CTD

185.

GeneCards

GC03P148415.

HGNC

HGNC:336. AGTR1.

HPA

HPA003596.

MIM

106165. gene.
267430. phenotype.

neXtProt

NX_P30556.

Orphanet

97369. Renal tubular dysgenesis of genetic origin.

PharmGKB

PA43.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG256015.

HOVERGEN

HBG104998.

InParanoid

P30556.

KO

K04166.

OMA

CIVIWLL.

OrthoDB

EOG48D0VX.

PhylomeDB

P30556.

Enzyme and pathway databases

Pathway_Interaction_DB

angiopoietinreceptor_pathway. Angiopoietin receptor Tie2-mediated signaling.
arf6cyclingpathway. Arf6 signaling events.

Reactome

REACT_111102. Signal Transduction.

Gene expression databases

ArrayExpress

P30556.

Bgee

P30556.

CleanEx

HS_AGTR1.

Genevestigator

P30556.

GermOnline

ENSG00000144891. Homo sapiens.

Family and domain databases

InterPro

IPR000190. ATII_AT1_rcpt.
IPR000248. ATII_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]

Pfam

PF00001. 7tm_1. 1 hit.
[Graphical view]

PRINTS

PR00241. ANGIOTENSINR.
PR00635. ANGIOTENSN1R.
PR00237. GPCRRHODOPSN.

PROSITE

PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

P30556.

ChEMBL

CHEMBL227.

DrugBank

DB00796. Candesartan.
DB00876. Eprosartan.
DB01342. Forasartan.
DB01029. Irbesartan.
DB00678. Losartan.
DB00275. Olmesartan.
DB01347. Saprisartan.
DB00421. Spironolactone.
DB01349. Tasosartan.
DB00966. Telmisartan.
DB00177. Valsartan.

GenomeRNAi

185.

NextBio

752.

SOURCE

Search...

Entry information

Entry name

AGTR1_HUMAN

Accession

Primary (citable) accession number: P30556
Secondary accession number(s): Q13725

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	May 1, 2013
This is version 136 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.