P30551 (CCKAR_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cholecystokinin receptor type A
Short name=CCK-A receptor
Short name=CCK-AR

Alternative name(s):
Cholecystokinin-1 receptor
Short name=CCK1-R

Gene names

Name:

Cckar

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	444 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for cholecystokinin. Mediates pancreatic growth and enzyme secretion, smooth muscle contraction of the gall bladder and stomach. Has a 1000-fold higher affinity for CCK rather than for gastrin. It modulates feeding and dopamine-induced behavior in the central and peripheral nervous system. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Ref.2
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Pancreas and brain. Also expressed in the gastrointestinal system and vagus nerve. Ref.2
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	actin cytoskeleton reorganization Inferred from direct assay PubMed 11891013. Source: RGD cellular response to hormone stimulus Inferred from expression pattern PubMed 12649564. Source: RGD elevation of cytosolic calcium ion concentration Inferred from direct assay PubMed 18776046. Source: RGD gastric acid secretion Inferred from direct assay PubMed 9773935. Source: RGD insulin secretion Inferred from direct assay PubMed 9773935. Source: RGD organ regeneration Inferred from expression pattern PubMed 10403848. Source: RGD pancreas development Inferred from expression pattern PubMed 10403848. Source: RGD pancreatic juice secretion Inferred from mutant phenotype PubMed 16327284. Source: RGD positive regulation of somatostatin secretion Inferred from mutant phenotype PubMed 19959964. Source: RGD reduction of food intake in response to dietary excess Inferred from mutant phenotype PubMed 12840200. Source: RGD regulation of calcium ion transport Inferred from direct assay PubMed 11207382. Source: RGD regulation of potassium ion transport Inferred from direct assay PubMed 12427859. Source: RGD response to glucocorticoid stimulus Inferred from expression pattern PubMed 7977738. Source: RGD response to heat Inferred from mutant phenotype PubMed 16891771. Source: RGD response to lipopolysaccharide Inferred from expression pattern PubMed 17622698. Source: RGD response to nutrient Inferred from expression pattern PubMed 15127944. Source: RGD response to radiation Inferred from expression pattern PubMed 16472889. Source: RGD response to starvation Inferred from mutant phenotype PubMed 12954406. Source: RGD temperature homeostasis Inferred from mutant phenotype PubMed 15178543. Source: RGD
Cellular_component	endoplasmic reticulum Inferred from direct assay PubMed 9169450. Source: RGD endosome Inferred from direct assay PubMed 9169450. Source: RGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW lysosome Inferred from direct assay PubMed 9169450. Source: RGD plasma membrane Inferred from direct assay PubMed 10535877. Source: RGD terminal bouton Inferred from direct assay PubMed 3408996. Source: RGD
Molecular_function	cholecystokinin receptor activity Inferred from direct assay PubMed 18776046. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 444	444	Cholecystokinin receptor type A		PRO_0000069226
Regions
	Topological domain	1 – 56	56	Extracellular Potential
	Transmembrane	57 – 82	26	Helical; Name=1; Potential
	Topological domain	83 – 92	10	Cytoplasmic Potential
	Transmembrane	93 – 119	27	Helical; Name=2; Potential
	Topological domain	120 – 130	11	Extracellular Potential
	Transmembrane	131 – 152	22	Helical; Name=3; Potential
	Topological domain	153 – 172	20	Cytoplasmic Potential
	Transmembrane	173 – 193	21	Helical; Name=4; Potential
	Topological domain	194 – 225	32	Extracellular Potential
	Transmembrane	226 – 249	24	Helical; Name=5; Potential
	Topological domain	250 – 329	80	Cytoplasmic Potential
	Transmembrane	330 – 350	21	Helical; Name=6; Potential
	Topological domain	351 – 365	15	Extracellular Potential
	Transmembrane	366 – 389	24	Helical; Name=7; Potential
	Topological domain	390 – 444	55	Cytoplasmic Potential
Amino acid modifications
	Lipidation	403	1	S-palmitoyl cysteine By similarity
	Glycosylation	25	1	N-linked (GlcNAc...) Potential
	Glycosylation	39	1	N-linked (GlcNAc...) Potential
	Glycosylation	205	1	N-linked (GlcNAc...) Potential
	Disulfide bond	33 ↔ 44		Ref.3
	Disulfide bond	129 ↔ 211		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P30551 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: B435BE7505C2FB11	FASTA	444	49,657
	10 20 30 40 50 60 MSHSPARQHL VESSRMDVVD SLLMNGSNIT PPCELGLENE TLFCLDQPQP SKEWQSALQI 70 80 90 100 110 120 LLYSIIFLLS VLGNTLVITV LIRNKRMRTV TNIFLLSLAV SDLMLCLFCM PFNLIPNLLK 130 140 150 160 170 180 DFIFGSAVCK TTTYFMGTSV SVSTFNLVAI SLERYGAICR PLQSRVWQTK SHALKVIAAT 190 200 210 220 230 240 WCLSFTIMTP YPIYSNLVPF TKNNNQTANM CRFLLPSDAM QQSWQTFLLL ILFLLPGIVM 250 260 270 280 290 300 VVAYGLISLE LYQGIKFDAS QKKSAKEKKP STGSSTRYED SDGCYLQKSR PPRKLELQQL 310 320 330 340 350 360 SSGSGGSRLN RIRSSSSAAN LIAKKRVIRM LIVIVVLFFL CWMPIFSANA WRAYDTVSAE 370 380 390 400 410 420 KHLSGTPISF ILLLSYTSSC VNPIIYCFMN KRFRLGFMAT FPCCPNPGPP GVRGEVGEEE 430 440 DGRTIRALLS RYSYSHMSTS APPP

References

[1]	"Purification, molecular cloning, and functional expression of the cholecystokinin receptor from rat pancreas." Wank S.A., Harkins R., Jensen R.T., Shapira H., de Weerth A., Slattery T. Proc. Natl. Acad. Sci. U.S.A. 89:3125-3129(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 111-158; 270-314 AND 392-402. Tissue: Pancreas.
[2]	"Gene structure of rat cholecystokinin type-A receptor." Takata Y., Takiguchi S., Funakoshi A., Kono A. Biochem. Biophys. Res. Commun. 213:958-966(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, FUNCTION.
[3]	"Disulfide bond structure and accessibility of cysteines in the ectodomain of the cholecystokinin receptor: specific mono-reactive receptor constructs examine charge-sensitivity of loop regions." Ding X.Q., Dolu V., Hadac E.M., Schuetz M., Miller L.J. Recept. Channels 9:83-91(2003) [PubMed] [Europe PMC] [Abstract] Cited for: DISULFIDE BONDS.
[4]	"The seventh transmembrane domain of gastrin/CCK receptors contributes to non-peptide antagonist binding." Mantamadiotis T., Baldwin G.S. Biochem. Biophys. Res. Commun. 201:1382-1389(1994) [PubMed] [Europe PMC] [Abstract] Cited for: ANTAGONIST BINDING.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M88096 mRNA. Translation: AAA40899.1. D50608 Genomic DNA. Translation: BAA09170.1.
IPI	IPI00210085.
PIR	A42685.
RefSeq	NP_036820.1. NM_012688.3.
UniGene	Rn.10184.
3D structure databases
ProteinModelPortal	P30551.
SMR	P30551. Positions 16-62, 345-373.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000063137.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P30551.
Proteomic databases
PRIDE	P30551.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	24889.
KEGG	rno:24889.
Organism-specific databases
CTD	886.
RGD	2289. Cckar.
Phylogenomic databases
eggNOG	NOG238659.
HOGENOM	HOG000198281.
HOVERGEN	HBG036927.
KO	K04194.
OrthoDB	EOG43N7D1.
Gene expression databases
ArrayExpress	P30551.
Genevestigator	P30551.
Family and domain databases
Gene3D	4.10.670.10. 1 hit.
InterPro	IPR009126. Cholcskin_rcpt. IPR000596. Cholcy_rcpt_A. IPR015276. CholecystokininA_recpt_N. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. PF09193. CholecysA-Rec_N. 1 hit. [Graphical view]
PRINTS	PR01822. CCYSTOKININR. PR00524. CCYSTOKNINAR. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P30551.
ChEMBL	CHEMBL2871.
NextBio	604752.
PMAP-CutDB	P30551.

Entry information

Entry name

CCKAR_RAT

Accession

Primary (citable) accession number: P30551

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	April 3, 2013
This is version 106 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program