P30548 (NK1R_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Substance-P receptor
Short name=SPR

Alternative name(s):
NK-1 receptor
Short name=NK-1R
Tachykinin receptor 1

Gene names

Name:	Tacr1
Synonyms:	Tac1r

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	407 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This is a receptor for the tachykinin neuropeptide substance P. It is probably associated with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of affinity of this receptor to tachykinins is: substance P > substance K > neuromedin K.
Subunit structure	Interacts with ARRB1 By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	acute inflammatory response Inferred from electronic annotation. Source: Compara aggressive behavior Inferred from electronic annotation. Source: Compara angiotensin-mediated drinking behavior Inferred from electronic annotation. Source: Compara associative learning Inferred from electronic annotation. Source: Compara behavioral response to pain Inferred from electronic annotation. Source: Compara eating behavior Inferred from electronic annotation. Source: Compara elevation of cytosolic calcium ion concentration Inferred from electronic annotation. Source: Compara long-term memory Inferred from electronic annotation. Source: Compara operant conditioning Inferred from electronic annotation. Source: Compara positive regulation of action potential Inferred from electronic annotation. Source: Compara positive regulation of blood pressure Inferred from electronic annotation. Source: Compara positive regulation of epithelial cell migration Inferred from electronic annotation. Source: Compara positive regulation of epithelial cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of hormone secretion Inferred from electronic annotation. Source: Compara positive regulation of leukocyte migration Inferred from electronic annotation. Source: Compara positive regulation of lymphocyte proliferation Inferred from electronic annotation. Source: Compara positive regulation of renal sodium excretion Inferred from electronic annotation. Source: Compara positive regulation of saliva secretion Inferred from electronic annotation. Source: Compara positive regulation of stress fiber assembly Inferred from electronic annotation. Source: Compara positive regulation of synaptic transmission, GABAergic Inferred from electronic annotation. Source: Compara positive regulation of synaptic transmission, cholinergic Inferred from electronic annotation. Source: Compara positive regulation of uterine smooth muscle contraction Inferred from electronic annotation. Source: Compara positive regulation of vascular permeability Inferred from electronic annotation. Source: Compara positive regulation of vasoconstriction Inferred from electronic annotation. Source: Compara regulation of smooth muscle cell migration Inferred from electronic annotation. Source: Compara regulation of smooth muscle cell proliferation Inferred from electronic annotation. Source: Compara response to auditory stimulus Inferred from electronic annotation. Source: Compara response to estradiol stimulus Inferred from electronic annotation. Source: Compara response to ethanol Inferred from electronic annotation. Source: Compara response to heat Inferred from electronic annotation. Source: Compara response to morphine Inferred from electronic annotation. Source: Compara response to nicotine Inferred from electronic annotation. Source: Compara response to ozone Inferred from electronic annotation. Source: Compara response to pain Inferred from mutant phenotype PubMed 11805341. Source: MGI response to progesterone stimulus Inferred from electronic annotation. Source: Compara smooth muscle contraction involved in micturition Inferred from electronic annotation. Source: Compara sperm ejaculation Inferred from electronic annotation. Source: Compara
Cellular_component	cell body Inferred from electronic annotation. Source: Compara cell periphery Inferred from direct assay PubMed 12383972. Source: MGI cell surface Inferred from electronic annotation. Source: Compara cytoplasm Inferred from electronic annotation. Source: Compara dendrite Inferred from electronic annotation. Source: Compara integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	substance P receptor activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 407	407	Substance-P receptor		PRO_0000069887
Regions
	Topological domain	1 – 31	31	Extracellular Potential
	Transmembrane	32 – 54	23	Helical; Name=1; Potential
	Topological domain	55 – 64	10	Cytoplasmic Potential
	Transmembrane	65 – 86	22	Helical; Name=2; Potential
	Topological domain	87 – 106	20	Extracellular Potential
	Transmembrane	107 – 128	22	Helical; Name=3; Potential
	Topological domain	129 – 148	20	Cytoplasmic Potential
	Transmembrane	149 – 169	21	Helical; Name=4; Potential
	Topological domain	170 – 194	25	Extracellular Potential
	Transmembrane	195 – 219	25	Helical; Name=5; Potential
	Topological domain	220 – 248	29	Cytoplasmic Potential
	Transmembrane	249 – 270	22	Helical; Name=6; Potential
	Topological domain	271 – 283	13	Extracellular Potential
	Transmembrane	284 – 308	25	Helical; Name=7; Potential
	Topological domain	309 – 407	99	Cytoplasmic Potential
Amino acid modifications
	Lipidation	322	1	S-palmitoyl cysteine Potential
	Glycosylation	14	1	N-linked (GlcNAc...) Potential
	Glycosylation	18	1	N-linked (GlcNAc...) Potential
	Disulfide bond	105 ↔ 180		By similarity
Experimental info
	Sequence conflict	405	1	M → I in CAA44707. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P30548 [UniParc]. Last modified July 27, 2011. Version 2. Checksum: 451B6B875A6659A0	FASTA	407	46,322
	10 20 30 40 50 60 MDNVLPVDSD LFPNTSTNTS ESNQFVQPTW QIVLWAAAYT VIVVTSVVGN VVVIWIILAH 70 80 90 100 110 120 KRMRTVTNYF LVNLAFAEAC MAAFNTVVNF TYAVHNVWYY GLFYCKFHNF FPIAALFASI 130 140 150 160 170 180 YSMTAVAFDR YMAIIHPLQP RLSATATKVV IFVIWVLALL LAFPQGYYST TETMPSRVVC 190 200 210 220 230 240 MIEWPEHPNR TYEKAYHICV TVLIYFLPLL VIGYAYTVVG ITLWASEIPG DSSDRYHEQV 250 260 270 280 290 300 SAKRKVVKMM IVVVCTFAIC WLPFHIFFLL PYINPDLYLK KFIQQVYLAS MWLAMSSTMY 310 320 330 340 350 360 NPIIYCCLND RFRLGFKHAF RCCPFISAGD YEGLEMKSTR YLQTQSSVYK VSRLETTIST 370 380 390 400 VVGAHEDEPE EGPKATPSSL DLTSNGSSRS NSKTMTESSS FYSNMLA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of the murine substance K and substance P receptor genes." Sundelin J.B., Provvedini D.M., Wahlestedt C.R., Laurell H., Pohl J.S., Peterson P.A. Eur. J. Biochem. 203:625-631(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Intestine.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Hypothalamus.
[3]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[5]	"Molecular evidence that granuloma T lymphocytes in murine schistosomiasis mansoni express an authentic substance P (NK-1) receptor." Cook G.A., Elliott D., Metwali A., Blum A.M., Sandor M., Lynch R., Weinstock J.V. J. Immunol. 152:1830-1835(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 63-290. Strain: CBA. Tissue: Brain and T-cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X62934 mRNA. Translation: CAA44707.1. AK038558 mRNA. Translation: BAC30042.1. CH466523 Genomic DNA. Translation: EDK99033.1. BC075631 mRNA. Translation: AAH75631.1. BC098332 mRNA. Translation: AAH98332.1. L27828 mRNA. Translation: AAA17892.1. L27826 mRNA. Translation: AAA17891.1.
IPI	IPI00131251.
PIR	S20304.
RefSeq	NP_033339.2. NM_009313.5.
UniGene	Mm.8055.
3D structure databases
ProteinModelPortal	P30548.
SMR	P30548. Positions 2-364.
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P30548.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000032122; ENSMUSP00000032122; ENSMUSG00000030043.
GeneID	21336.
KEGG	mmu:21336.
Organism-specific databases
CTD	6869.
MGI	MGI:98475. Tacr1.
Phylogenomic databases
eggNOG	NOG331485.
GeneTree	ENSGT00610000085813.
HOGENOM	HOG000013018.
HOVERGEN	HBG103412.
InParanoid	Q8BYR7.
KO	K04222.
OMA	LPFHVFF.
OrthoDB	EOG4WQ12N.
Gene expression databases
ArrayExpress	P30548.
Bgee	P30548.
CleanEx	MM_TACR1.
Genevestigator	P30548.
GermOnline	ENSMUSG00000030043. Mus musculus.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR001681. Neurokn_rcpt. IPR000046. NK1_rcpt. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR01024. NEUROKININ1R. PR00244. NEUROKININR.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P30548.
ChEMBL	CHEMBL2668.
NextBio	300500.
SOURCE	Search...

Entry information

Entry name

NK1R_MOUSE

Accession

Primary (citable) accession number: P30548
Secondary accession number(s): Q8BYR7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	July 27, 2011
Last modified:	April 3, 2013
This is version 98 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program