Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P30548 (NK1R_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Substance-P receptor

Short name=SPR
Alternative name(s):
NK-1 receptor
Short name=NK-1R
Tachykinin receptor 1
Gene names
Name:Tacr1
Synonyms:Tac1r
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length407 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

This is a receptor for the tachykinin neuropeptide substance P. It is probably associated with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of affinity of this receptor to tachykinins is: substance P > substance K > neuromedin K.

Subunit structure

Interacts with ARRB1 By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
Lipoprotein
Palmitate
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processacute inflammatory response

Inferred from electronic annotation. Source: Ensembl

aggressive behavior

Inferred from electronic annotation. Source: Ensembl

angiotensin-mediated drinking behavior

Inferred from electronic annotation. Source: Ensembl

associative learning

Inferred from electronic annotation. Source: Ensembl

behavioral response to pain

Inferred from electronic annotation. Source: Ensembl

eating behavior

Inferred from electronic annotation. Source: Ensembl

long-term memory

Inferred from electronic annotation. Source: Ensembl

operant conditioning

Inferred from electronic annotation. Source: Ensembl

positive regulation of action potential

Inferred from electronic annotation. Source: Ensembl

positive regulation of blood pressure

Inferred from electronic annotation. Source: Ensembl

positive regulation of cytosolic calcium ion concentration

Inferred from electronic annotation. Source: Ensembl

positive regulation of epithelial cell migration

Inferred from electronic annotation. Source: Ensembl

positive regulation of epithelial cell proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of hormone secretion

Inferred from electronic annotation. Source: Ensembl

positive regulation of leukocyte migration

Inferred from electronic annotation. Source: Ensembl

positive regulation of lymphocyte proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of ossification

Inferred from electronic annotation. Source: Ensembl

positive regulation of renal sodium excretion

Inferred from electronic annotation. Source: Ensembl

positive regulation of saliva secretion

Inferred from electronic annotation. Source: Ensembl

positive regulation of stress fiber assembly

Inferred from electronic annotation. Source: Ensembl

positive regulation of synaptic transmission, GABAergic

Inferred from electronic annotation. Source: Ensembl

positive regulation of synaptic transmission, cholinergic

Inferred from electronic annotation. Source: Ensembl

positive regulation of uterine smooth muscle contraction

Inferred from electronic annotation. Source: Ensembl

positive regulation of vascular permeability

Inferred from electronic annotation. Source: Ensembl

positive regulation of vasoconstriction

Inferred from electronic annotation. Source: Ensembl

regulation of smooth muscle cell migration

Inferred from electronic annotation. Source: Ensembl

regulation of smooth muscle cell proliferation

Inferred from electronic annotation. Source: Ensembl

response to auditory stimulus

Inferred from electronic annotation. Source: Ensembl

response to estradiol

Inferred from electronic annotation. Source: Ensembl

response to ethanol

Inferred from electronic annotation. Source: Ensembl

response to heat

Inferred from electronic annotation. Source: Ensembl

response to morphine

Inferred from electronic annotation. Source: Ensembl

response to nicotine

Inferred from electronic annotation. Source: Ensembl

response to ozone

Inferred from electronic annotation. Source: Ensembl

response to pain

Inferred from mutant phenotype PubMed 11805341. Source: MGI

response to progesterone

Inferred from electronic annotation. Source: Ensembl

smooth muscle contraction involved in micturition

Inferred from electronic annotation. Source: Ensembl

sperm ejaculation

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcell body

Inferred from electronic annotation. Source: Ensembl

cell periphery

Inferred from direct assay PubMed 12383972. Source: MGI

cell surface

Inferred from electronic annotation. Source: Ensembl

cytoplasm

Inferred from electronic annotation. Source: Ensembl

dendrite

Inferred from electronic annotation. Source: Ensembl

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionsubstance P receptor activity

Inferred from electronic annotation. Source: Ensembl

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 407407Substance-P receptor
PRO_0000069887

Regions

Topological domain1 – 3131Extracellular Potential
Transmembrane32 – 5423Helical; Name=1; Potential
Topological domain55 – 6410Cytoplasmic Potential
Transmembrane65 – 8622Helical; Name=2; Potential
Topological domain87 – 10620Extracellular Potential
Transmembrane107 – 12822Helical; Name=3; Potential
Topological domain129 – 14820Cytoplasmic Potential
Transmembrane149 – 16921Helical; Name=4; Potential
Topological domain170 – 19425Extracellular Potential
Transmembrane195 – 21925Helical; Name=5; Potential
Topological domain220 – 24829Cytoplasmic Potential
Transmembrane249 – 27022Helical; Name=6; Potential
Topological domain271 – 28313Extracellular Potential
Transmembrane284 – 30825Helical; Name=7; Potential
Topological domain309 – 40799Cytoplasmic Potential

Amino acid modifications

Lipidation3221S-palmitoyl cysteine Potential
Glycosylation141N-linked (GlcNAc...) Potential
Glycosylation181N-linked (GlcNAc...) Potential
Disulfide bond105 ↔ 180 By similarity

Experimental info

Sequence conflict4051M → I in CAA44707. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P30548 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 451B6B875A6659A0

FASTA40746,322
        10         20         30         40         50         60 
MDNVLPVDSD LFPNTSTNTS ESNQFVQPTW QIVLWAAAYT VIVVTSVVGN VVVIWIILAH 

        70         80         90        100        110        120 
KRMRTVTNYF LVNLAFAEAC MAAFNTVVNF TYAVHNVWYY GLFYCKFHNF FPIAALFASI 

       130        140        150        160        170        180 
YSMTAVAFDR YMAIIHPLQP RLSATATKVV IFVIWVLALL LAFPQGYYST TETMPSRVVC 

       190        200        210        220        230        240 
MIEWPEHPNR TYEKAYHICV TVLIYFLPLL VIGYAYTVVG ITLWASEIPG DSSDRYHEQV 

       250        260        270        280        290        300 
SAKRKVVKMM IVVVCTFAIC WLPFHIFFLL PYINPDLYLK KFIQQVYLAS MWLAMSSTMY 

       310        320        330        340        350        360 
NPIIYCCLND RFRLGFKHAF RCCPFISAGD YEGLEMKSTR YLQTQSSVYK VSRLETTIST 

       370        380        390        400 
VVGAHEDEPE EGPKATPSSL DLTSNGSSRS NSKTMTESSS FYSNMLA 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning of the murine substance K and substance P receptor genes."
Sundelin J.B., Provvedini D.M., Wahlestedt C.R., Laurell H., Pohl J.S., Peterson P.A.
Eur. J. Biochem. 203:625-631(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Intestine.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Hypothalamus.
[3]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
[5]"Molecular evidence that granuloma T lymphocytes in murine schistosomiasis mansoni express an authentic substance P (NK-1) receptor."
Cook G.A., Elliott D., Metwali A., Blum A.M., Sandor M., Lynch R., Weinstock J.V.
J. Immunol. 152:1830-1835(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 63-290.
Strain: CBA.
Tissue: Brain and T-cell.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X62934 mRNA. Translation: CAA44707.1.
AK038558 mRNA. Translation: BAC30042.1.
CH466523 Genomic DNA. Translation: EDK99033.1.
BC075631 mRNA. Translation: AAH75631.1.
BC098332 mRNA. Translation: AAH98332.1.
L27828 mRNA. Translation: AAA17892.1.
L27826 mRNA. Translation: AAA17891.1.
PIRS20304.
RefSeqNP_033339.2. NM_009313.5.
XP_006505926.1. XM_006505863.1.
XP_006505927.1. XM_006505864.1.
XP_006505928.1. XM_006505865.1.
UniGeneMm.8055.

3D structure databases

ProteinModelPortalP30548.
SMRP30548. Positions 2-364.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBP30548.
ChEMBLCHEMBL2668.
GuidetoPHARMACOLOGY360.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP30548.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000032122; ENSMUSP00000032122; ENSMUSG00000030043.
GeneID21336.
KEGGmmu:21336.
UCSCuc009clh.2. mouse.

Organism-specific databases

CTD6869.
MGIMGI:98475. Tacr1.

Phylogenomic databases

eggNOGNOG331485.
GeneTreeENSGT00710000106471.
HOGENOMHOG000013018.
HOVERGENHBG103412.
InParanoidQ8BYR7.
KOK04222.
OMAMIIVVCT.
OrthoDBEOG7N37FW.
TreeFamTF315303.

Gene expression databases

ArrayExpressP30548.
BgeeP30548.
CleanExMM_TACR1.
GenevestigatorP30548.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001681. Neurokn_rcpt.
IPR000046. NK1_rcpt.
[Graphical view]
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00237. GPCRRHODOPSN.
PR01024. NEUROKININ1R.
PR00244. NEUROKININR.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio300500.
PROP30548.
SOURCESearch...

Entry information

Entry nameNK1R_MOUSE
AccessionPrimary (citable) accession number: P30548
Secondary accession number(s): Q8BYR7
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: July 27, 2011
Last modified: April 16, 2014
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries