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P30545

- ADA2B_MOUSE

UniProt

P30545 - ADA2B_MOUSE

Protein

Alpha-2B adrenergic receptor

Gene

Adra2b

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 126 (01 Oct 2014)
      Sequence version 2 (21 Jun 2005)
      Previous versions | rss
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    Functioni

    Alpha-2 adrenergic receptors mediate the catecholamine-induced inhibition of adenylate cyclase through the action of G proteins.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei92 – 921Implicated in ligand bindingBy similarity
    Sitei176 – 1761Implicated in catechol agonist bindingBy similarity
    Sitei180 – 1801Implicated in catechol agonist bindingBy similarity

    GO - Molecular functioni

    1. alpha2-adrenergic receptor activity Source: InterPro
    2. protein binding Source: IntAct

    GO - Biological processi

    1. platelet activation Source: InterPro
    2. regulation of smooth muscle contraction Source: InterPro
    3. regulation of vasoconstriction Source: InterPro

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-2B adrenergic receptor
    Alternative name(s):
    Alpha-2B adrenoreceptor
    Short name:
    Alpha-2B adrenoceptor
    Short name:
    Alpha-2BAR
    Gene namesi
    Name:Adra2b
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:87935. Adra2b.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 450450Alpha-2B adrenergic receptorPRO_0000069096Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi85 ↔ 164PROSITE-ProRule annotation
    Lipidationi442 – 4421S-palmitoyl cysteineSequence Analysis

    Keywords - PTMi

    Disulfide bond, Lipoprotein, Palmitate

    Proteomic databases

    PaxDbiP30545.
    PRIDEiP30545.

    Expressioni

    Gene expression databases

    CleanExiMM_ADRA2B.
    GenevestigatoriP30545.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    EIF2B1Q142322EBI-491084,EBI-491065From a different organism.

    Protein-protein interaction databases

    IntActiP30545. 2 interactions.
    MINTiMINT-4087111.
    STRINGi10090.ENSMUSP00000071798.

    Structurei

    3D structure databases

    ProteinModelPortaliP30545.
    SMRiP30545. Positions 2-441.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1212ExtracellularBy similarityAdd
    BLAST
    Topological domaini38 – 4912CytoplasmicBy similarityAdd
    BLAST
    Topological domaini76 – 8510ExtracellularBy similarity
    Topological domaini109 – 13022CytoplasmicBy similarityAdd
    BLAST
    Topological domaini154 – 16916ExtracellularBy similarityAdd
    BLAST
    Topological domaini194 – 372179CytoplasmicBy similarityAdd
    BLAST
    Topological domaini397 – 4059ExtracellularBy similarity
    Topological domaini430 – 45021CytoplasmicBy similarityAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei13 – 3725Helical; Name=1By similarityAdd
    BLAST
    Transmembranei50 – 7526Helical; Name=2By similarityAdd
    BLAST
    Transmembranei86 – 10823Helical; Name=3By similarityAdd
    BLAST
    Transmembranei131 – 15323Helical; Name=4By similarityAdd
    BLAST
    Transmembranei170 – 19324Helical; Name=5By similarityAdd
    BLAST
    Transmembranei373 – 39624Helical; Name=6By similarityAdd
    BLAST
    Transmembranei406 – 42924Helical; Name=7By similarityAdd
    BLAST

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi301 – 31111Asp/Glu-rich (acidic)Add
    BLAST

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA2B sub-subfamily.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG249628.
    HOGENOMiHOG000239242.
    HOVERGENiHBG106962.
    InParanoidiP30545.
    PhylomeDBiP30545.

    Family and domain databases

    Gene3Di1.20.1070.10. 2 hits.
    InterProiIPR002233. ADR_fam.
    IPR000207. ADRA2B_rcpt.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    [Graphical view]
    PANTHERiPTHR24248:SF26. PTHR24248:SF26. 1 hit.
    PfamiPF00001. 7tm_1. 1 hit.
    [Graphical view]
    PRINTSiPR01103. ADRENERGICR.
    PR00559. ADRENRGCA2BR.
    PR00237. GPCRRHODOPSN.
    PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P30545-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVHQEPYSVQ ATAAIASAIT FLILFTIFGN ALVILAVLTS RSLRAPQNLF    50
    LVSLAAADIL VATLIIPFSL ANELLGYWYF WRAWCEVYLA LDVLFCTSSI 100
    VHLCAISLDR YWAVSRALEY NSKRTPRRIK CIILTVWLIA AVISLPPLIY 150
    KGDQRPEPHG LPQCELNQEA WYILASSIGS FFAPCLIMIL VYLRIYVIAK 200
    RSHCRGLGAK RGSGEGESKK PRPGPAAGGV PASAKVPTLV SPLSSVGEAN 250
    GHPKPPREKE EGETPEDPEA RALPPNWSAL PRSVQDQKKG TSGATAEKGA 300
    EEDEEEVEEC EPQTLPASPA SVFNPPLQQP QTSRVLATLR GQVLLSKNVG 350
    VASGQWWRRR TQLSREKRFT FVLAVVIGVF VVCWFPFFFS YSLGAICPQH 400
    CKVPHGLFQF FFWIGYCNSS LNPVIYTIFN QDFRRAFRRI LCRQWTQTGW 450
    Length:450
    Mass (Da):50,172
    Last modified:June 21, 2005 - v2
    Checksum:iA95DA296CA12A992
    GO

    Sequence cautioni

    The sequence AAA73895.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti197 – 1971V → L in AAA73895. (PubMed:1336396)Curated
    Sequence conflicti224 – 2252Missing(PubMed:1354956)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M94583 Genomic DNA. Translation: AAA73895.1. Different initiation.
    L00979 Genomic DNA. Translation: AAA37131.1.
    PIRiS28221.
    UniGeneiMm.347390.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M94583 Genomic DNA. Translation: AAA73895.1 . Different initiation.
    L00979 Genomic DNA. Translation: AAA37131.1 .
    PIRi S28221.
    UniGenei Mm.347390.

    3D structure databases

    ProteinModelPortali P30545.
    SMRi P30545. Positions 2-441.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P30545. 2 interactions.
    MINTi MINT-4087111.
    STRINGi 10090.ENSMUSP00000071798.

    Chemistry

    BindingDBi P30545.
    ChEMBLi CHEMBL2405.
    GuidetoPHARMACOLOGYi 26.

    Protein family/group databases

    GPCRDBi Search...

    Proteomic databases

    PaxDbi P30545.
    PRIDEi P30545.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    MGIi MGI:87935. Adra2b.

    Phylogenomic databases

    eggNOGi NOG249628.
    HOGENOMi HOG000239242.
    HOVERGENi HBG106962.
    InParanoidi P30545.
    PhylomeDBi P30545.

    Miscellaneous databases

    PROi P30545.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_ADRA2B.
    Genevestigatori P30545.

    Family and domain databases

    Gene3Di 1.20.1070.10. 2 hits.
    InterProi IPR002233. ADR_fam.
    IPR000207. ADRA2B_rcpt.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    [Graphical view ]
    PANTHERi PTHR24248:SF26. PTHR24248:SF26. 1 hit.
    Pfami PF00001. 7tm_1. 1 hit.
    [Graphical view ]
    PRINTSi PR01103. ADRENERGICR.
    PR00559. ADRENRGCA2BR.
    PR00237. GPCRRHODOPSN.
    PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterization of a mouse alpha 2C2 adrenoceptor subtype gene."
      Chen W.-M., Chang A.C., Shie B.J., Chang Y.-H., Chang N.-C.A.
      Biochim. Biophys. Acta 1171:219-223(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: DBA/2.
      Tissue: Liver.
    2. "Cloning and expression of the mouse homolog of the human alpha 2-C2 adrenergic receptor."
      Chruscinski A.J., Link R.E., Daunt D.A., Barsh G.S., Kobilka B.K.
      Biochem. Biophys. Res. Commun. 186:1280-1287(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiADA2B_MOUSE
    AccessioniPrimary (citable) accession number: P30545
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: June 21, 2005
    Last modified: October 1, 2014
    This is version 126 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3