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Protein

Alpha-2B adrenergic receptor

Gene

Adra2b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Alpha-2 adrenergic receptors mediate the catecholamine-induced inhibition of adenylate cyclase through the action of G proteins.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei92 – 921Implicated in ligand bindingBy similarity
Sitei176 – 1761Implicated in catechol agonist bindingBy similarity
Sitei180 – 1801Implicated in catechol agonist bindingBy similarity

GO - Molecular functioni

  1. alpha2-adrenergic receptor activity Source: InterPro

GO - Biological processi

  1. platelet activation Source: InterPro
  2. regulation of smooth muscle contraction Source: InterPro
  3. regulation of vasoconstriction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-2B adrenergic receptor
Alternative name(s):
Alpha-2B adrenoreceptor
Short name:
Alpha-2B adrenoceptor
Short name:
Alpha-2BAR
Gene namesi
Name:Adra2b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:87935. Adra2b.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1212ExtracellularBy similarityAdd
BLAST
Transmembranei13 – 3725Helical; Name=1By similarityAdd
BLAST
Topological domaini38 – 4912CytoplasmicBy similarityAdd
BLAST
Transmembranei50 – 7526Helical; Name=2By similarityAdd
BLAST
Topological domaini76 – 8510ExtracellularBy similarity
Transmembranei86 – 10823Helical; Name=3By similarityAdd
BLAST
Topological domaini109 – 13022CytoplasmicBy similarityAdd
BLAST
Transmembranei131 – 15323Helical; Name=4By similarityAdd
BLAST
Topological domaini154 – 16916ExtracellularBy similarityAdd
BLAST
Transmembranei170 – 19324Helical; Name=5By similarityAdd
BLAST
Topological domaini194 – 372179CytoplasmicBy similarityAdd
BLAST
Transmembranei373 – 39624Helical; Name=6By similarityAdd
BLAST
Topological domaini397 – 4059ExtracellularBy similarity
Transmembranei406 – 42924Helical; Name=7By similarityAdd
BLAST
Topological domaini430 – 45021CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 450450Alpha-2B adrenergic receptorPRO_0000069096Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi85 ↔ 164PROSITE-ProRule annotation
Lipidationi442 – 4421S-palmitoyl cysteineSequence Analysis

Keywords - PTMi

Disulfide bond, Lipoprotein, Palmitate

Proteomic databases

PaxDbiP30545.
PRIDEiP30545.

Expressioni

Gene expression databases

CleanExiMM_ADRA2B.
GenevestigatoriP30545.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
EIF2B1Q142322EBI-491084,EBI-491065From a different organism.

Protein-protein interaction databases

IntActiP30545. 2 interactions.
MINTiMINT-4087111.
STRINGi10090.ENSMUSP00000071798.

Structurei

3D structure databases

ProteinModelPortaliP30545.
SMRiP30545. Positions 2-441.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi301 – 31111Asp/Glu-rich (acidic)Add
BLAST

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA2B sub-subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG249628.
HOGENOMiHOG000239242.
HOVERGENiHBG106962.
InParanoidiP30545.
PhylomeDBiP30545.

Family and domain databases

InterProiIPR002233. ADR_fam.
IPR000207. ADRA2B_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24248:SF26. PTHR24248:SF26. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR01103. ADRENERGICR.
PR00559. ADRENRGCA2BR.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P30545-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVHQEPYSVQ ATAAIASAIT FLILFTIFGN ALVILAVLTS RSLRAPQNLF
60 70 80 90 100
LVSLAAADIL VATLIIPFSL ANELLGYWYF WRAWCEVYLA LDVLFCTSSI
110 120 130 140 150
VHLCAISLDR YWAVSRALEY NSKRTPRRIK CIILTVWLIA AVISLPPLIY
160 170 180 190 200
KGDQRPEPHG LPQCELNQEA WYILASSIGS FFAPCLIMIL VYLRIYVIAK
210 220 230 240 250
RSHCRGLGAK RGSGEGESKK PRPGPAAGGV PASAKVPTLV SPLSSVGEAN
260 270 280 290 300
GHPKPPREKE EGETPEDPEA RALPPNWSAL PRSVQDQKKG TSGATAEKGA
310 320 330 340 350
EEDEEEVEEC EPQTLPASPA SVFNPPLQQP QTSRVLATLR GQVLLSKNVG
360 370 380 390 400
VASGQWWRRR TQLSREKRFT FVLAVVIGVF VVCWFPFFFS YSLGAICPQH
410 420 430 440 450
CKVPHGLFQF FFWIGYCNSS LNPVIYTIFN QDFRRAFRRI LCRQWTQTGW
Length:450
Mass (Da):50,172
Last modified:June 21, 2005 - v2
Checksum:iA95DA296CA12A992
GO

Sequence cautioni

The sequence AAA73895.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti197 – 1971V → L in AAA73895 (PubMed:1336396).Curated
Sequence conflicti224 – 2252Missing (PubMed:1354956).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M94583 Genomic DNA. Translation: AAA73895.1. Different initiation.
L00979 Genomic DNA. Translation: AAA37131.1.
PIRiS28221.
UniGeneiMm.347390.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M94583 Genomic DNA. Translation: AAA73895.1. Different initiation.
L00979 Genomic DNA. Translation: AAA37131.1.
PIRiS28221.
UniGeneiMm.347390.

3D structure databases

ProteinModelPortaliP30545.
SMRiP30545. Positions 2-441.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP30545. 2 interactions.
MINTiMINT-4087111.
STRINGi10090.ENSMUSP00000071798.

Chemistry

BindingDBiP30545.
ChEMBLiCHEMBL2405.
GuidetoPHARMACOLOGYi26.

Protein family/group databases

GPCRDBiSearch...

Proteomic databases

PaxDbiP30545.
PRIDEiP30545.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:87935. Adra2b.

Phylogenomic databases

eggNOGiNOG249628.
HOGENOMiHOG000239242.
HOVERGENiHBG106962.
InParanoidiP30545.
PhylomeDBiP30545.

Miscellaneous databases

PROiP30545.
SOURCEiSearch...

Gene expression databases

CleanExiMM_ADRA2B.
GenevestigatoriP30545.

Family and domain databases

InterProiIPR002233. ADR_fam.
IPR000207. ADRA2B_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24248:SF26. PTHR24248:SF26. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR01103. ADRENERGICR.
PR00559. ADRENRGCA2BR.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and characterization of a mouse alpha 2C2 adrenoceptor subtype gene."
    Chen W.-M., Chang A.C., Shie B.J., Chang Y.-H., Chang N.-C.A.
    Biochim. Biophys. Acta 1171:219-223(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: DBA/2.
    Tissue: Liver.
  2. "Cloning and expression of the mouse homolog of the human alpha 2-C2 adrenergic receptor."
    Chruscinski A.J., Link R.E., Daunt D.A., Barsh G.S., Kobilka B.K.
    Biochem. Biophys. Res. Commun. 186:1280-1287(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiADA2B_MOUSE
AccessioniPrimary (citable) accession number: P30545
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: June 21, 2005
Last modified: January 7, 2015
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.