Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P30545 (ADA2B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-2B adrenergic receptor
Alternative name(s):
Alpha-2B adrenoreceptor
Short name=Alpha-2B adrenoceptor
Short name=Alpha-2BAR
Gene names
Name:Adra2b
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length450 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Alpha-2 adrenergic receptors mediate the catecholamine-induced inhibition of adenylate cyclase through the action of G proteins.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRA2B sub-subfamily.

Sequence caution

The sequence AAA73895.1 differs from that shown. Reason: Erroneous initiation.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

EIF2B1Q142322EBI-491084,EBI-491065From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 450450Alpha-2B adrenergic receptor
PRO_0000069096

Regions

Topological domain1 – 1212Extracellular By similarity
Transmembrane13 – 3725Helical; Name=1; By similarity
Topological domain38 – 4912Cytoplasmic By similarity
Transmembrane50 – 7526Helical; Name=2; By similarity
Topological domain76 – 8510Extracellular By similarity
Transmembrane86 – 10823Helical; Name=3; By similarity
Topological domain109 – 13022Cytoplasmic By similarity
Transmembrane131 – 15323Helical; Name=4; By similarity
Topological domain154 – 16916Extracellular By similarity
Transmembrane170 – 19324Helical; Name=5; By similarity
Topological domain194 – 372179Cytoplasmic By similarity
Transmembrane373 – 39624Helical; Name=6; By similarity
Topological domain397 – 4059Extracellular By similarity
Transmembrane406 – 42924Helical; Name=7; By similarity
Topological domain430 – 45021Cytoplasmic By similarity
Compositional bias301 – 31111Asp/Glu-rich (acidic)

Sites

Site921Implicated in ligand binding By similarity
Site1761Implicated in catechol agonist binding By similarity
Site1801Implicated in catechol agonist binding By similarity

Amino acid modifications

Lipidation4421S-palmitoyl cysteine Potential
Disulfide bond85 ↔ 164 By similarity

Experimental info

Sequence conflict1971V → L in AAA73895. Ref.1
Sequence conflict224 – 2252Missing Ref.2

Sequences

Sequence LengthMass (Da)Tools
P30545 [UniParc].

Last modified June 21, 2005. Version 2.
Checksum: A95DA296CA12A992

FASTA45050,172
        10         20         30         40         50         60 
MVHQEPYSVQ ATAAIASAIT FLILFTIFGN ALVILAVLTS RSLRAPQNLF LVSLAAADIL 

        70         80         90        100        110        120 
VATLIIPFSL ANELLGYWYF WRAWCEVYLA LDVLFCTSSI VHLCAISLDR YWAVSRALEY 

       130        140        150        160        170        180 
NSKRTPRRIK CIILTVWLIA AVISLPPLIY KGDQRPEPHG LPQCELNQEA WYILASSIGS 

       190        200        210        220        230        240 
FFAPCLIMIL VYLRIYVIAK RSHCRGLGAK RGSGEGESKK PRPGPAAGGV PASAKVPTLV 

       250        260        270        280        290        300 
SPLSSVGEAN GHPKPPREKE EGETPEDPEA RALPPNWSAL PRSVQDQKKG TSGATAEKGA 

       310        320        330        340        350        360 
EEDEEEVEEC EPQTLPASPA SVFNPPLQQP QTSRVLATLR GQVLLSKNVG VASGQWWRRR 

       370        380        390        400        410        420 
TQLSREKRFT FVLAVVIGVF VVCWFPFFFS YSLGAICPQH CKVPHGLFQF FFWIGYCNSS 

       430        440        450 
LNPVIYTIFN QDFRRAFRRI LCRQWTQTGW 

« Hide

References

[1]"Molecular cloning and characterization of a mouse alpha 2C2 adrenoceptor subtype gene."
Chen W.-M., Chang A.C., Shie B.J., Chang Y.-H., Chang N.-C.A.
Biochim. Biophys. Acta 1171:219-223(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: DBA/2.
Tissue: Liver.
[2]"Cloning and expression of the mouse homolog of the human alpha 2-C2 adrenergic receptor."
Chruscinski A.J., Link R.E., Daunt D.A., Barsh G.S., Kobilka B.K.
Biochem. Biophys. Res. Commun. 186:1280-1287(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M94583 Genomic DNA. Translation: AAA73895.1. Different initiation.
L00979 Genomic DNA. Translation: AAA37131.1.
PIRS28221.
UniGeneMm.347390.

3D structure databases

ProteinModelPortalP30545.
SMRP30545. Positions 16-443.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP30545. 2 interactions.
MINTMINT-4087111.
STRING10090.ENSMUSP00000071798.

Chemistry

BindingDBP30545.
ChEMBLCHEMBL2405.
GuidetoPHARMACOLOGY26.

Protein family/group databases

GPCRDBSearch...

Proteomic databases

PaxDbP30545.
PRIDEP30545.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

MGIMGI:87935. Adra2b.

Phylogenomic databases

eggNOGNOG249628.
HOGENOMHOG000239242.
HOVERGENHBG106962.
InParanoidP30545.
PhylomeDBP30545.

Gene expression databases

CleanExMM_ADRA2B.
GenevestigatorP30545.

Family and domain databases

Gene3D1.20.1070.10. 2 hits.
InterProIPR002233. ADR_fam.
IPR000207. ADRA2B_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERPTHR24248. PTHR24248. 1 hit.
PTHR24248:SF26. PTHR24248:SF26. 1 hit.
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR01103. ADRENERGICR.
PR00559. ADRENRGCA2BR.
PR00237. GPCRRHODOPSN.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP30545.
SOURCESearch...

Entry information

Entry nameADA2B_MOUSE
AccessionPrimary (citable) accession number: P30545
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: June 21, 2005
Last modified: April 16, 2014
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries