P30543 (AA2AR_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenosine receptor A2a

Gene names

Name:

Adora2a

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	410 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
Subunit structure	Interacts (via cytoplasmic C-terminal domain) with USP4; the interaction is direct By similarity. May interact with DRD4 By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein Ref.6.
Domain	The cytoplasmic C-terminal domain is necessary for targeting the non-ubiquitinated form of this protein to the cell surface By similarity.
Post-translational modification	Ubiquitinated. Deubiquitinated by USP4; leading to stabilization and expression at the cell surface By similarity.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	adenylate cyclase-activating G-protein coupled receptor signaling pathway Inferred from mutant phenotype PubMed 16478979. Source: RGD astrocyte activation Inferred from mutant phenotype PubMed 12838511. Source: RGD eating behavior Inferred from mutant phenotype PubMed 12827647. Source: RGD locomotory behavior Inferred from mutant phenotype PubMed 12827647. Source: RGD negative regulation of alpha-beta T cell activation Inferred from electronic annotation. Source: Compara negative regulation of cell proliferation Inferred from direct assay PubMed 16617164. Source: RGD negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Inferred from direct assay PubMed 15211592. Source: RGD negative regulation of inflammatory response Inferred from electronic annotation. Source: Compara negative regulation of locomotion Inferred from direct assay PubMed 12189203. Source: RGD negative regulation of neuron apoptotic process Inferred from direct assay PubMed 15211592. Source: RGD negative regulation of protein kinase activity Inferred from direct assay PubMed 12590138. Source: RGD negative regulation of vascular permeability Inferred from mutant phenotype PubMed 16815983. Source: RGD neuron projection morphogenesis Inferred from direct assay PubMed 16617164. Source: RGD positive regulation of acetylcholine secretion Inferred from mutant phenotype PubMed 12679375. Source: RGD positive regulation of apoptotic signaling pathway Inferred from electronic annotation. Source: Compara positive regulation of cAMP biosynthetic process Inferred from electronic annotation. Source: Compara positive regulation of circadian sleep/wake cycle, sleep Inferred from direct assay PubMed 16109808. Source: RGD positive regulation of glutamate secretion Inferred from direct assay PubMed 16481441. Source: RGD positive regulation of protein secretion Inferred from direct assay PubMed 14742743. Source: RGD positive regulation of renal sodium excretion Inferred from direct assay PubMed 10070140. Source: RGD positive regulation of synaptic transmission, GABAergic Inferred from electronic annotation. Source: Compara positive regulation of synaptic transmission, glutamatergic Inferred from direct assay PubMed 16481441. Source: RGD positive regulation of urine volume Inferred from mutant phenotype PubMed 10070140. Source: RGD prepulse inhibition Inferred from mutant phenotype PubMed 12827647. Source: RGD protein kinase C-activating G-protein coupled receptor signaling pathway Inferred from direct assay PubMed 12590138. Source: RGD regulation of calcium ion transport Inferred from mutant phenotype PubMed 15297571. Source: RGD regulation of excitatory postsynaptic membrane potential Inferred from direct assay PubMed 16644907. Source: RGD regulation of inhibitory postsynaptic membrane potential Inferred from electronic annotation. Source: Compara regulation of mitochondrial membrane potential Inferred from direct assay PubMed 15211592. Source: RGD regulation of norepinephrine secretion Inferred from mutant phenotype PubMed 16539684. Source: RGD regulation of synaptic plasticity Inferred from direct assay PubMed 14596853. Source: RGD regulation of transcription, DNA-dependent Inferred from direct assay PubMed 12189203. Source: RGD response to amphetamine Inferred from electronic annotation. Source: Compara response to caffeine Inferred from direct assay PubMed 16481441. Source: RGD response to drug Inferred from expression pattern PubMed 17853654. Source: RGD synaptic transmission, cholinergic Inferred from mutant phenotype PubMed 15297571. Source: RGD synaptic transmission, dopaminergic Inferred from electronic annotation. Source: Compara synaptic transmission, glutamatergic Inferred from direct assay PubMed 16644907. Source: RGD vasodilation Inferred from mutant phenotype PubMed 10070140PubMed 16601261. Source: RGD
Cellular_component	Golgi membrane Traceable author statement. Source: Reactome asymmetric synapse Inferred from direct assay PubMed 16481441. Source: RGD axolemma Inferred from direct assay PubMed 16481441. Source: RGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW neuronal cell body Inferred from direct assay PubMed 16644907. Source: RGD postsynaptic density Inferred from direct assay PubMed 15659214. Source: RGD postsynaptic membrane Inferred from direct assay PubMed 16481441. Source: RGD presynaptic active zone Inferred from direct assay PubMed 16481441. Source: RGD presynaptic membrane Inferred from direct assay PubMed 16481441. Source: RGD
Molecular_function	G-protein coupled adenosine receptor activity Inferred from direct assay PubMed 12590138. Source: RGD alpha-actinin binding Inferred from direct assay PubMed 12837758. Source: RGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Cnr1	P20272	3	EBI-2902822,EBI-2909800
Tsnax	Q9QZE7	6	EBI-2902822,EBI-2910751	From a different organism.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 410	410	Adenosine receptor A2a		PRO_0000069001
Regions
	Topological domain	1 – 4	4	Extracellular By similarity
	Transmembrane	5 – 29	25	Helical; Name=1; By similarity
	Topological domain	30 – 39	10	Cytoplasmic By similarity
	Transmembrane	40 – 63	24	Helical; Name=2; By similarity
	Topological domain	64 – 74	11	Extracellular By similarity
	Transmembrane	75 – 97	23	Helical; Name=3; By similarity
	Topological domain	98 – 117	20	Cytoplasmic By similarity
	Transmembrane	118 – 140	23	Helical; Name=4; By similarity
	Topological domain	141 – 168	28	Extracellular By similarity
	Transmembrane	169 – 193	25	Helical; Name=5; By similarity
	Topological domain	194 – 229	36	Cytoplasmic By similarity
	Transmembrane	230 – 253	24	Helical; Name=6; By similarity
	Topological domain	254 – 261	8	Extracellular By similarity
	Transmembrane	262 – 285	24	Helical; Name=7; By similarity
	Topological domain	286 – 410	125	Cytoplasmic By similarity
	Region	163 – 172	10	Agonist binding By similarity
	Region	241 – 248	8	Agonist binding By similarity
	Region	259 – 269	11	Agonist binding By similarity
Amino acid modifications
	Glycosylation	142	1	N-linked (GlcNAc...) Potential
	Glycosylation	149	1	N-linked (GlcNAc...) Potential
	Disulfide bond	68 ↔ 154		By similarity
	Disulfide bond	71 ↔ 143		By similarity
	Disulfide bond	74 ↔ 161		By similarity
	Disulfide bond	254 ↔ 257		By similarity
Experimental info
	Sequence conflict	268	1	A → T in AAA70305. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P30543 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: D87B256CD2336BAC	FASTA	410	44,999
	10 20 30 40 50 60 MGSSVYITVE LAIAVLAILG NVLVCWAVWI NSNLQNVTNF FVVSLAAADI AVGVLAIPFA 70 80 90 100 110 120 ITISTGFCAA CHGCLFFACF VLVLTQSSIF SLLAIAIDRY IAIRIPLRYN GLVTGVRAKG 130 140 150 160 170 180 IIAICWVLSF AIGLTPMLGW NNCSQKDGNS TKTCGEGRVT CLFEDVVPMN YMVYYNFFAF 190 200 210 220 230 240 VLLPLLLMLA IYLRIFLAAR RQLKQMESQP LPGERTRSTL QKEVHAAKSL AIIVGLFALC 250 260 270 280 290 300 WLPLHIINCF TFFCSTCRHA PPWLMYLAII LSHSNSVVNP FIYAYRIREF RQTFRKIIRT 310 320 330 340 350 360 HVLRRQEPFQ AGGSSAWALA AHSTEGEQVS LRLNGHPLGV WANGSATHSG RRPNGYTLGL 370 380 390 400 410 GGGGSAQGSP RDVELPTQER QEGQEHPGLR GHLVQARVGA SSWSSEFAPS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of a novel adenosine receptor gene from rat brain." Chern Y., King K., Lai H.-L., Lai H.-T. Biochem. Biophys. Res. Commun. 185:304-309(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	Chern Y. Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 183. Tissue: Brain.
[3]	"Molecular cloning of the rat A2 adenosine receptor: selective co-expression with D2 dopamine receptors in rat striatum." Fink J.S., Weaver D.R., Rivkees S.A., Peterfreund R.A., Pollack A.E., Adler E.M., Reppert S.M. Brain Res. Mol. Brain Res. 14:186-195(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Corpus striatum.
[4]	"Rattus norvegicus mucosa A2A adenosine receptor mRNA." Yip L., Hewitt J., Kwok Y. Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[6]	"The ubiquitin-specific protease Usp4 regulates the cell surface level of the A2A receptor." Milojevic T., Reiterer V., Stefan E., Korkhov V.M., Dorostkar M.M., Ducza E., Ogris E., Boehm S., Freissmuth M., Nanoff C. Mol. Pharmacol. 69:1083-1094(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M91214 mRNA. Translation: AAA70305.2. L08102 mRNA. No translation available. S47609 mRNA. Translation: AAA11888.1. AF228684 mRNA. Translation: AAF61924.1. BC081727 mRNA. Translation: AAH81727.1.
IPI	IPI00327229.
PIR	A48974.
RefSeq	NP_445746.3. NM_053294.3.
UniGene	Rn.11180.
3D structure databases
ProteinModelPortal	P30543.
ModBase	Search...
Protein-protein interaction databases
IntAct	P30543. 6 interactions.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P30543.
Proteomic databases
PaxDb	P30543.
PRIDE	P30543.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000001759; ENSRNOP00000001759; ENSRNOG00000001302.
GeneID	25369.
KEGG	rno:25369.
UCSC	RGD:2049. rat.
Organism-specific databases
CTD	135.
RGD	2049. Adora2a.
Phylogenomic databases
eggNOG	NOG287824.
GeneTree	ENSGT00550000074524.
HOGENOM	HOG000015770.
HOVERGEN	HBG106962.
InParanoid	P30543.
KO	K04266.
OMA	PGVWANG.
OrthoDB	EOG44XJHH.
Enzyme and pathway databases
Reactome	REACT_111984. Signal Transduction.
Gene expression databases
ArrayExpress	P30543.
Genevestigator	P30543.
GermOnline	ENSRNOG00000001302. Rattus norvegicus.
Family and domain databases
InterPro	IPR001513. Adeno_A2A_rcpt. IPR001634. Adenosn_rcpt. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00553. ADENOSINA2AR. PR00424. ADENOSINER. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P30543.
ChEMBL	CHEMBL302.
NextBio	606377.

Entry information

Entry name

AA2AR_RAT

Accession

Primary (citable) accession number: P30543

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	December 1, 2000
Last modified:	April 3, 2013
This is version 108 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program