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Protein

Adenosine receptor A2a

Gene

Adora2a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.

GO - Molecular functioni

  • alpha-actinin binding Source: RGD
  • G-protein coupled adenosine receptor activity Source: RGD
  • protein heterodimerization activity Source: RGD
  • type 5 metabotropic glutamate receptor binding Source: RGD

GO - Biological processi

  • adenosine receptor signaling pathway Source: RGD
  • adenylate cyclase-activating G-protein coupled receptor signaling pathway Source: RGD
  • astrocyte activation Source: RGD
  • eating behavior Source: RGD
  • excitatory postsynaptic potential Source: RGD
  • inhibitory postsynaptic potential Source: Ensembl
  • locomotory behavior Source: RGD
  • membrane depolarization Source: RGD
  • negative regulation of alpha-beta T cell activation Source: Ensembl
  • negative regulation of cell proliferation Source: RGD
  • negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: RGD
  • negative regulation of inflammatory response Source: Ensembl
  • negative regulation of locomotion Source: RGD
  • negative regulation of neuron apoptotic process Source: RGD
  • negative regulation of protein kinase activity Source: RGD
  • negative regulation of vascular permeability Source: RGD
  • neuron projection morphogenesis Source: RGD
  • positive regulation of acetylcholine secretion, neurotransmission Source: RGD
  • positive regulation of apoptotic signaling pathway Source: Ensembl
  • positive regulation of cAMP biosynthetic process Source: Ensembl
  • positive regulation of circadian sleep/wake cycle, sleep Source: RGD
  • positive regulation of glutamate secretion Source: RGD
  • positive regulation of protein secretion Source: RGD
  • positive regulation of renal sodium excretion Source: RGD
  • positive regulation of synaptic transmission, GABAergic Source: Ensembl
  • positive regulation of synaptic transmission, glutamatergic Source: RGD
  • positive regulation of urine volume Source: RGD
  • prepulse inhibition Source: RGD
  • protein kinase C-activating G-protein coupled receptor signaling pathway Source: RGD
  • regulation of calcium ion transport Source: RGD
  • regulation of mitochondrial membrane potential Source: RGD
  • regulation of norepinephrine secretion Source: RGD
  • regulation of synaptic plasticity Source: RGD
  • regulation of transcription, DNA-templated Source: RGD
  • response to alkaloid Source: RGD
  • response to amphetamine Source: Ensembl
  • response to caffeine Source: RGD
  • response to drug Source: RGD
  • synaptic transmission, cholinergic Source: RGD
  • synaptic transmission, dopaminergic Source: Ensembl
  • synaptic transmission, glutamatergic Source: RGD
  • vasodilation Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-RNO-417973. Adenosine P1 receptors.
R-RNO-418555. G alpha (s) signalling events.
R-RNO-5683826. Surfactant metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenosine receptor A2a
Gene namesi
Name:Adora2a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 20

Organism-specific databases

RGDi2049. Adora2a.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 44ExtracellularBy similarity
Transmembranei5 – 2925Helical; Name=1By similarityAdd
BLAST
Topological domaini30 – 3910CytoplasmicBy similarity
Transmembranei40 – 6324Helical; Name=2By similarityAdd
BLAST
Topological domaini64 – 7411ExtracellularBy similarityAdd
BLAST
Transmembranei75 – 9723Helical; Name=3By similarityAdd
BLAST
Topological domaini98 – 11720CytoplasmicBy similarityAdd
BLAST
Transmembranei118 – 14023Helical; Name=4By similarityAdd
BLAST
Topological domaini141 – 16828ExtracellularBy similarityAdd
BLAST
Transmembranei169 – 19325Helical; Name=5By similarityAdd
BLAST
Topological domaini194 – 22936CytoplasmicBy similarityAdd
BLAST
Transmembranei230 – 25324Helical; Name=6By similarityAdd
BLAST
Topological domaini254 – 2618ExtracellularBy similarity
Transmembranei262 – 28524Helical; Name=7By similarityAdd
BLAST
Topological domaini286 – 410125CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • asymmetric synapse Source: RGD
  • axolemma Source: RGD
  • axon Source: RGD
  • dendrite Source: RGD
  • endomembrane system Source: RGD
  • Golgi membrane Source: Reactome
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: RGD
  • neuronal cell body Source: RGD
  • plasma membrane Source: RGD
  • postsynaptic density Source: RGD
  • postsynaptic membrane Source: RGD
  • presynaptic active zone Source: RGD
  • presynaptic membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2094117.
GuidetoPHARMACOLOGYi19.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 410410Adenosine receptor A2aPRO_0000069001Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi68 ↔ 154PROSITE-ProRule annotation
Disulfide bondi71 ↔ 143PROSITE-ProRule annotation
Disulfide bondi74 ↔ 161PROSITE-ProRule annotation
Glycosylationi142 – 1421N-linked (GlcNAc...)Sequence analysis
Glycosylationi149 – 1491N-linked (GlcNAc...)Sequence analysis
Disulfide bondi254 ↔ 257PROSITE-ProRule annotation

Post-translational modificationi

Ubiquitinated. Deubiquitinated by USP4; leading to stabilization and expression at the cell surface (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Ubl conjugation

Proteomic databases

PaxDbiP30543.
PRIDEiP30543.

PTM databases

PhosphoSiteiP30543.

Expressioni

Gene expression databases

GenevisibleiP30543. RN.

Interactioni

Subunit structurei

Interacts (via cytoplasmic C-terminal domain) with USP4; the interaction is direct. May interact with DRD4.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Cnr1P202723EBI-2902822,EBI-2909800
TsnaxQ9QZE76EBI-2902822,EBI-2910751From a different organism.

GO - Molecular functioni

  • alpha-actinin binding Source: RGD
  • protein heterodimerization activity Source: RGD
  • type 5 metabotropic glutamate receptor binding Source: RGD

Protein-protein interaction databases

BioGridi247408. 2 interactions.
IntActiP30543. 6 interactions.
MINTiMINT-269383.
STRINGi10116.ENSRNOP00000001759.

Chemistry

BindingDBiP30543.

Structurei

3D structure databases

ProteinModelPortaliP30543.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni163 – 17210Agonist bindingBy similarity
Regioni241 – 2488Agonist bindingBy similarity
Regioni259 – 26911Agonist bindingBy similarityAdd
BLAST

Domaini

The cytoplasmic C-terminal domain is necessary for targeting the non-ubiquitinated form of this protein to the cell surface.By similarity

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00550000074524.
HOGENOMiHOG000015770.
HOVERGENiHBG106962.
InParanoidiP30543.
KOiK04266.
OMAiPGVWANG.
OrthoDBiEOG79SDXQ.
PhylomeDBiP30543.
TreeFamiTF325296.

Family and domain databases

InterProiIPR001513. Adeno_A2A_rcpt.
IPR001634. Adenosn_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24246:SF28. PTHR24246:SF28. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00553. ADENOSINA2AR.
PR00424. ADENOSINER.
PR00237. GPCRRHODOPSN.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P30543-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSSVYITVE LAIAVLAILG NVLVCWAVWI NSNLQNVTNF FVVSLAAADI
60 70 80 90 100
AVGVLAIPFA ITISTGFCAA CHGCLFFACF VLVLTQSSIF SLLAIAIDRY
110 120 130 140 150
IAIRIPLRYN GLVTGVRAKG IIAICWVLSF AIGLTPMLGW NNCSQKDGNS
160 170 180 190 200
TKTCGEGRVT CLFEDVVPMN YMVYYNFFAF VLLPLLLMLA IYLRIFLAAR
210 220 230 240 250
RQLKQMESQP LPGERTRSTL QKEVHAAKSL AIIVGLFALC WLPLHIINCF
260 270 280 290 300
TFFCSTCRHA PPWLMYLAII LSHSNSVVNP FIYAYRIREF RQTFRKIIRT
310 320 330 340 350
HVLRRQEPFQ AGGSSAWALA AHSTEGEQVS LRLNGHPLGV WANGSATHSG
360 370 380 390 400
RRPNGYTLGL GGGGSAQGSP RDVELPTQER QEGQEHPGLR GHLVQARVGA
410
SSWSSEFAPS
Length:410
Mass (Da):44,999
Last modified:December 1, 2000 - v2
Checksum:iD87B256CD2336BAC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti268 – 2681A → T in AAA70305 (PubMed:1599465).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M91214 mRNA. Translation: AAA70305.2.
L08102 mRNA. No translation available.
S47609 mRNA. Translation: AAA11888.1.
AF228684 mRNA. Translation: AAF61924.1.
BC081727 mRNA. Translation: AAH81727.1.
PIRiA48974.
RefSeqiNP_445746.3. NM_053294.4.
UniGeneiRn.11180.

Genome annotation databases

EnsembliENSRNOT00000001759; ENSRNOP00000001759; ENSRNOG00000001302.
GeneIDi25369.
KEGGirno:25369.
UCSCiRGD:2049. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M91214 mRNA. Translation: AAA70305.2.
L08102 mRNA. No translation available.
S47609 mRNA. Translation: AAA11888.1.
AF228684 mRNA. Translation: AAF61924.1.
BC081727 mRNA. Translation: AAH81727.1.
PIRiA48974.
RefSeqiNP_445746.3. NM_053294.4.
UniGeneiRn.11180.

3D structure databases

ProteinModelPortaliP30543.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi247408. 2 interactions.
IntActiP30543. 6 interactions.
MINTiMINT-269383.
STRINGi10116.ENSRNOP00000001759.

Chemistry

BindingDBiP30543.
ChEMBLiCHEMBL2094117.
GuidetoPHARMACOLOGYi19.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiP30543.

Proteomic databases

PaxDbiP30543.
PRIDEiP30543.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000001759; ENSRNOP00000001759; ENSRNOG00000001302.
GeneIDi25369.
KEGGirno:25369.
UCSCiRGD:2049. rat.

Organism-specific databases

CTDi135.
RGDi2049. Adora2a.

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00550000074524.
HOGENOMiHOG000015770.
HOVERGENiHBG106962.
InParanoidiP30543.
KOiK04266.
OMAiPGVWANG.
OrthoDBiEOG79SDXQ.
PhylomeDBiP30543.
TreeFamiTF325296.

Enzyme and pathway databases

ReactomeiR-RNO-417973. Adenosine P1 receptors.
R-RNO-418555. G alpha (s) signalling events.
R-RNO-5683826. Surfactant metabolism.

Miscellaneous databases

NextBioi606377.
PROiP30543.

Gene expression databases

GenevisibleiP30543. RN.

Family and domain databases

InterProiIPR001513. Adeno_A2A_rcpt.
IPR001634. Adenosn_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24246:SF28. PTHR24246:SF28. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00553. ADENOSINA2AR.
PR00424. ADENOSINER.
PR00237. GPCRRHODOPSN.
SMARTiSM01381. 7TM_GPCR_Srsx. 1 hit.
[Graphical view]
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of a novel adenosine receptor gene from rat brain."
    Chern Y., King K., Lai H.-L., Lai H.-T.
    Biochem. Biophys. Res. Commun. 185:304-309(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. Chern Y.
    Submitted (JUN-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 183.
    Tissue: Brain.
  3. "Molecular cloning of the rat A2 adenosine receptor: selective co-expression with D2 dopamine receptors in rat striatum."
    Fink J.S., Weaver D.R., Rivkees S.A., Peterfreund R.A., Pollack A.E., Adler E.M., Reppert S.M.
    Brain Res. Mol. Brain Res. 14:186-195(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Corpus striatum.
  4. "Rattus norvegicus mucosa A2A adenosine receptor mRNA."
    Yip L., Hewitt J., Kwok Y.
    Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.
  6. "The ubiquitin-specific protease Usp4 regulates the cell surface level of the A2A receptor."
    Milojevic T., Reiterer V., Stefan E., Korkhov V.M., Dorostkar M.M., Ducza E., Ogris E., Boehm S., Freissmuth M., Nanoff C.
    Mol. Pharmacol. 69:1083-1094(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiAA2AR_RAT
AccessioniPrimary (citable) accession number: P30543
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: December 1, 2000
Last modified: May 11, 2016
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.