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Protein

Adenosine receptor A2a

Gene

Adora2a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for adenosine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.

GO - Molecular functioni

  • alpha-actinin binding Source: RGD
  • enzyme binding Source: Ensembl
  • G-protein coupled adenosine receptor activity Source: RGD
  • identical protein binding Source: Ensembl
  • protein heterodimerization activity Source: RGD
  • type 5 metabotropic glutamate receptor binding Source: RGD

GO - Biological processi

  • activation of adenylate cyclase activity Source: Reactome
  • adenosine receptor signaling pathway Source: RGD
  • adenylate cyclase-activating G-protein coupled receptor signaling pathway Source: RGD
  • astrocyte activation Source: RGD
  • eating behavior Source: RGD
  • excitatory postsynaptic potential Source: RGD
  • inhibitory postsynaptic potential Source: Ensembl
  • locomotory behavior Source: RGD
  • membrane depolarization Source: RGD
  • negative regulation of alpha-beta T cell activation Source: Ensembl
  • negative regulation of cell proliferation Source: RGD
  • negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: RGD
  • negative regulation of inflammatory response Source: Ensembl
  • negative regulation of locomotion Source: RGD
  • negative regulation of neuron apoptotic process Source: RGD
  • negative regulation of protein kinase activity Source: RGD
  • negative regulation of vascular permeability Source: RGD
  • neuron projection morphogenesis Source: RGD
  • positive regulation of acetylcholine secretion, neurotransmission Source: RGD
  • positive regulation of adenylate cyclase activity involved in G-protein coupled receptor signaling pathway Source: Ensembl
  • positive regulation of apoptotic signaling pathway Source: Ensembl
  • positive regulation of circadian sleep/wake cycle, sleep Source: RGD
  • positive regulation of glutamate secretion Source: RGD
  • positive regulation of protein secretion Source: RGD
  • positive regulation of renal sodium excretion Source: RGD
  • positive regulation of synaptic transmission, GABAergic Source: Ensembl
  • positive regulation of synaptic transmission, glutamatergic Source: RGD
  • positive regulation of urine volume Source: RGD
  • prepulse inhibition Source: RGD
  • protein kinase C-activating G-protein coupled receptor signaling pathway Source: RGD
  • regulation of calcium ion transport Source: RGD
  • regulation of mitochondrial membrane potential Source: RGD
  • regulation of norepinephrine secretion Source: RGD
  • regulation of synaptic plasticity Source: RGD
  • regulation of transcription, DNA-templated Source: RGD
  • response to alkaloid Source: RGD
  • response to amphetamine Source: Ensembl
  • response to caffeine Source: RGD
  • response to drug Source: RGD
  • synaptic transmission, cholinergic Source: RGD
  • synaptic transmission, dopaminergic Source: Ensembl
  • synaptic transmission, glutamatergic Source: RGD
  • vasodilation Source: RGD

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-RNO-187024. NGF-independant TRKA activation.
R-RNO-417973. Adenosine P1 receptors.
R-RNO-418555. G alpha (s) signalling events.
R-RNO-5683826. Surfactant metabolism.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenosine receptor A2a
Gene namesi
Name:Adora2a
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 20

Organism-specific databases

RGDi2049. Adora2a.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 4ExtracellularBy similarity4
Transmembranei5 – 29Helical; Name=1By similarityAdd BLAST25
Topological domaini30 – 39CytoplasmicBy similarity10
Transmembranei40 – 63Helical; Name=2By similarityAdd BLAST24
Topological domaini64 – 74ExtracellularBy similarityAdd BLAST11
Transmembranei75 – 97Helical; Name=3By similarityAdd BLAST23
Topological domaini98 – 117CytoplasmicBy similarityAdd BLAST20
Transmembranei118 – 140Helical; Name=4By similarityAdd BLAST23
Topological domaini141 – 168ExtracellularBy similarityAdd BLAST28
Transmembranei169 – 193Helical; Name=5By similarityAdd BLAST25
Topological domaini194 – 229CytoplasmicBy similarityAdd BLAST36
Transmembranei230 – 253Helical; Name=6By similarityAdd BLAST24
Topological domaini254 – 261ExtracellularBy similarity8
Transmembranei262 – 285Helical; Name=7By similarityAdd BLAST24
Topological domaini286 – 410CytoplasmicBy similarityAdd BLAST125

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL302.
GuidetoPHARMACOLOGYi19.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000690011 – 410Adenosine receptor A2aAdd BLAST410

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi68 ↔ 154PROSITE-ProRule annotation
Disulfide bondi71 ↔ 143PROSITE-ProRule annotation
Disulfide bondi74 ↔ 161PROSITE-ProRule annotation
Glycosylationi142N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi149N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi254 ↔ 257PROSITE-ProRule annotation

Post-translational modificationi

Ubiquitinated. Deubiquitinated by USP4; leading to stabilization and expression at the cell surface (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Ubl conjugation

Proteomic databases

PaxDbiP30543.
PRIDEiP30543.

PTM databases

PhosphoSitePlusiP30543.

Expressioni

Gene expression databases

BgeeiENSRNOG00000001302.
GenevisibleiP30543. RN.

Interactioni

Subunit structurei

Interacts (via cytoplasmic C-terminal domain) with USP4; the interaction is direct. May interact with DRD4. Interacts with NECAB2 (By similarity).By similarity

Binary interactionsi

Show more details

GO - Molecular functioni

  • alpha-actinin binding Source: RGD
  • enzyme binding Source: Ensembl
  • identical protein binding Source: Ensembl
  • protein heterodimerization activity Source: RGD
  • type 5 metabotropic glutamate receptor binding Source: RGD

Protein-protein interaction databases

BioGridi247408. 2 interactors.
IntActiP30543. 6 interactors.
MINTiMINT-269383.
STRINGi10116.ENSRNOP00000001759.

Chemistry databases

BindingDBiP30543.

Structurei

3D structure databases

ProteinModelPortaliP30543.
SMRiP30543.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni163 – 172Agonist bindingBy similarity10
Regioni241 – 248Agonist bindingBy similarity8
Regioni259 – 269Agonist bindingBy similarityAdd BLAST11

Domaini

The cytoplasmic C-terminal domain is necessary for targeting the non-ubiquitinated form of this protein to the cell surface.By similarity

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3656. Eukaryota.
ENOG410XRW9. LUCA.
GeneTreeiENSGT00550000074524.
HOGENOMiHOG000015770.
HOVERGENiHBG106962.
InParanoidiP30543.
KOiK04266.
OMAiMESQPLP.
OrthoDBiEOG091G0I3U.
PhylomeDBiP30543.
TreeFamiTF325296.

Family and domain databases

CDDicd15068. 7tmA_Adenosine_R_A2A. 1 hit.
InterProiView protein in InterPro
IPR001513. Adeno_A2A_rcpt.
IPR001634. Adenosn_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
PANTHERiPTHR24246:SF32. PTHR24246:SF32. 1 hit.
PfamiView protein in Pfam
PF00001. 7tm_1. 1 hit.
PRINTSiPR00553. ADENOSINA2AR.
PR00424. ADENOSINER.
PR00237. GPCRRHODOPSN.
SMARTiView protein in SMART
SM01381. 7TM_GPCR_Srsx. 1 hit.
PROSITEiView protein in PROSITE
PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P30543-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSSVYITVE LAIAVLAILG NVLVCWAVWI NSNLQNVTNF FVVSLAAADI
60 70 80 90 100
AVGVLAIPFA ITISTGFCAA CHGCLFFACF VLVLTQSSIF SLLAIAIDRY
110 120 130 140 150
IAIRIPLRYN GLVTGVRAKG IIAICWVLSF AIGLTPMLGW NNCSQKDGNS
160 170 180 190 200
TKTCGEGRVT CLFEDVVPMN YMVYYNFFAF VLLPLLLMLA IYLRIFLAAR
210 220 230 240 250
RQLKQMESQP LPGERTRSTL QKEVHAAKSL AIIVGLFALC WLPLHIINCF
260 270 280 290 300
TFFCSTCRHA PPWLMYLAII LSHSNSVVNP FIYAYRIREF RQTFRKIIRT
310 320 330 340 350
HVLRRQEPFQ AGGSSAWALA AHSTEGEQVS LRLNGHPLGV WANGSATHSG
360 370 380 390 400
RRPNGYTLGL GGGGSAQGSP RDVELPTQER QEGQEHPGLR GHLVQARVGA
410
SSWSSEFAPS
Length:410
Mass (Da):44,999
Last modified:December 1, 2000 - v2
Checksum:iD87B256CD2336BAC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti268A → T in AAA70305 (PubMed:1599465).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M91214 mRNA. Translation: AAA70305.2.
L08102 mRNA. No translation available.
S47609 mRNA. Translation: AAA11888.1.
AF228684 mRNA. Translation: AAF61924.1.
BC081727 mRNA. Translation: AAH81727.1.
PIRiA48974.
RefSeqiNP_445746.3. NM_053294.4.
XP_017457041.1. XM_017601552.1.
XP_017457042.1. XM_017601553.1.
XP_017457043.1. XM_017601554.1.
XP_017457044.1. XM_017601555.1.
UniGeneiRn.11180.

Genome annotation databases

EnsembliENSRNOT00000001759; ENSRNOP00000001759; ENSRNOG00000001302.
GeneIDi25369.
KEGGirno:25369.
UCSCiRGD:2049. rat.

Similar proteinsi

Entry informationi

Entry nameiAA2AR_RAT
AccessioniPrimary (citable) accession number: P30543
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: December 1, 2000
Last modified: September 27, 2017
This is version 148 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families