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P30542 (AA1R_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Adenosine receptor A1
Gene names
Name:ADORA1
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length326 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

ADORA2AP292744EBI-2903663,EBI-2902702

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P30542-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P30542-2)

The sequence of this isoform differs from the canonical sequence as follows:
     115-125: YKMVVTPRRAA → RISQCMASTKS
     126-326: Missing.
Note: Gene prediction based on EST data.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 326326Adenosine receptor A1
PRO_0000068991

Regions

Topological domain1 – 1010Extracellular Potential
Transmembrane11 – 3323Helical; Name=1; Potential
Topological domain34 – 4613Cytoplasmic Potential
Transmembrane47 – 6923Helical; Name=2; Potential
Topological domain70 – 8011Extracellular Potential
Transmembrane81 – 10222Helical; Name=3; Potential
Topological domain103 – 12321Cytoplasmic Potential
Transmembrane124 – 14623Helical; Name=4; Potential
Topological domain147 – 17630Extracellular Potential
Transmembrane177 – 20125Helical; Name=5; Potential
Topological domain202 – 23534Cytoplasmic Potential
Transmembrane236 – 25924Helical; Name=6; Potential
Topological domain260 – 2678Extracellular Potential
Transmembrane268 – 29225Helical; Name=7; Potential
Topological domain293 – 32634Cytoplasmic Potential

Amino acid modifications

Lipidation3091S-palmitoyl cysteine Potential
Glycosylation1591N-linked (GlcNAc...) Potential
Disulfide bond80 ↔ 169 By similarity

Natural variations

Alternative sequence115 – 12511YKMVVTPRRAA → RISQCMASTKS in isoform 2.
VSP_034401
Alternative sequence126 – 326201Missing in isoform 2.
VSP_034402
Natural variant431A → S. Ref.12
Corresponds to variant rs11547175 [ dbSNP | Ensembl ].
VAR_044138
Natural variant501S → P. Ref.12
Corresponds to variant rs11547174 [ dbSNP | Ensembl ].
VAR_044139
Natural variant1051R → H. Ref.12
Corresponds to variant rs11547176 [ dbSNP | Ensembl ].
VAR_044140
Natural variant1701E → K in a colorectal cancer sample; somatic mutation. Ref.13
VAR_035754
Natural variant2611P → Q. Ref.12
Corresponds to variant rs17852405 [ dbSNP | Ensembl ].
VAR_044141

Experimental info

Sequence conflict3121A → T in BAF82617. Ref.6

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: 1B555893BCDEC9A6

FASTA32636,512
        10         20         30         40         50         60 
MPPSISAFQA AYIGIEVLIA LVSVPGNVLV IWAVKVNQAL RDATFCFIVS LAVADVAVGA 

        70         80         90        100        110        120 
LVIPLAILIN IGPQTYFHTC LMVACPVLIL TQSSILALLA IAVDRYLRVK IPLRYKMVVT 

       130        140        150        160        170        180 
PRRAAVAIAG CWILSFVVGL TPMFGWNNLS AVERAWAANG SMGEPVIKCE FEKVISMEYM 

       190        200        210        220        230        240 
VYFNFFVWVL PPLLLMVLIY LEVFYLIRKQ LNKKVSASSG DPQKYYGKEL KIAKSLALIL 

       250        260        270        280        290        300 
FLFALSWLPL HILNCITLFC PSCHKPSILT YIAIFLTHGN SAMNPIVYAF RIQKFRVTFL 

       310        320 
KIWNDHFRCQ PAPPIDEDLP EERPDD

« Hide

Isoform 2 [UniParc].

Checksum: 37CB91365D17A5F5
Show »

FASTA12513,348

References

« Hide 'large scale' references
[1]"Cloning and functional characterization of a human A1 adenosine receptor."
Libert F., van Sande J., Lefort A., Czernilofsky A., Dumont J.E., Vassart G., Ensinger H.A., Mendla K.D.
Biochem. Biophys. Res. Commun. 187:919-926(1992) [PubMed: 1530647] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Hippocampus.
[2]"Molecular cloning and characterisation of a human brain A1 adenosine receptor cDNA."
Townsend-Nicholson A., Shine J.
Brain Res. Mol. Brain Res. 16:365-370(1992) [PubMed: 1339301] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Brain.
[3]"Characterization of the human A1 adenosine receptor gene. Evidence for alternative splicing."
Ren H., Stiles G.L.
J. Biol. Chem. 269:3104-3110(1994) [PubMed: 8300646] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Hippocampus.
[4]Salvatore C.A., Luneau C.J., Johnson R.G., Jacobson M.
Submitted (SEP-1992) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Brain.
[5]"Complete nucleotide sequence of an adenosine A1-receptor from heart."
Hirabatake Y., Takao K., Hagiwara S., Kasanuki H., Hosoda S., Kokubun S.
Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Heart.
[6]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain and Hippocampus.
[7]"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
Puhl H.L. III, Ikeda S.R., Aronstam R.S.
Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
[8]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[9]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[10]"The DNA sequence and biological annotation of human chromosome 1."
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. expand/collapse author list , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.
Nature 441:315-321(2006) [PubMed: 16710414] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[11]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[12]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS SER-43; PRO-50; HIS-105 AND GLN-261.
Tissue: Brain.
[13]"The consensus coding sequences of human breast and colorectal cancers."
Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. expand/collapse author list , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E.
Science 314:268-274(2006) [PubMed: 16959974] [Abstract]
Cited for: VARIANT [LARGE SCALE ANALYSIS] LYS-170.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		S45235 mRNA. Translation: AAB23388.1.
S56143 mRNA. Translation: AAB25533.2.
L22214 mRNA. Translation: AAA17544.1.
X68485 mRNA. Translation: CAA48503.1.
AB004662 mRNA. Translation: BAA20433.1.
AK127752 mRNA. Translation: BAG54566.1.
AK289928 mRNA. Translation: BAF82617.1.
AY136746 mRNA. Translation: AAN01272.1.
BT019854 mRNA. Translation: AAV38657.1.
CR541749 mRNA. Translation: CAG46549.1.
AC105940 Genomic DNA. No translation available.
CH471067 Genomic DNA. Translation: EAW91465.1.
BC026340 mRNA. Translation: AAH26340.1.
IPIIPI00026872.
IPI00645335.
PIRA53005.
RefSeqNP_000665.1. NM_000674.2.
NP_001041695.1. NM_001048230.1.
UniGeneHs.77867.

3D structure databases

ProteinModelPortalP30542.
SMRP30542. Positions 10-319.
ModBaseSearch...

Protein-protein interaction databases

IntActP30542. 2 interactions.
MINTMINT-138736.
STRINGP30542.

Protein family/group databases

GPCRDBSearch...

Polymorphism databases

DMDM231473.

Proteomic databases

PRIDEP30542.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000309502; ENSP00000308549; ENSG00000163485.
ENST00000337894; ENSP00000338435; ENSG00000163485.
ENST00000367236; ENSP00000356205; ENSG00000163485.
GeneID134.
KEGGhsa:134.
UCSCuc001gze.1. human.

Organism-specific databases

CTD134.
GeneCardsGC01P203059.
H-InvDBHIX0001486.
HGNCHGNC:262. ADORA1.
MIM102775. gene.
neXtProtNX_P30542.
PharmGKBPA24583.
GenAtlasSearch...

Phylogenomic databases

eggNOGprNOG07376.
GeneTreeENSGT00550000074524.
HOVERGENHBG106962.
InParanoidP30542.
OMAIAGCWIL.
OrthoDBEOG4BVRV7.
PhylomeDBP30542.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.

Gene expression databases

ArrayExpressP30542.
BgeeP30542.
CleanExHS_ADORA1.
GenevestigatorP30542.
GermOnlineENSG00000163485. Homo sapiens.

Family and domain databases

InterProIPR000276. 7TM_GPCR_Rhodpsn.
IPR001068. Adeno_A1_rcpt.
IPR001634. Adenosn_rcpt.
IPR017452. GPCR_Rhodpsn_supfam.
[Graphical view]
KOK04265.
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00552. ADENOSINEA1R.
PR00424. ADENOSINER.
PR00237. GPCRRHODOPSN.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

DrugBankDB01223. Aminophylline.
DB00201. Caffeine.
DB04932. Defibrotide.
DB00996. Gabapentin.
DB00458. Imipramine.
DB00061. Pegademase bovine.
DB00277. Theophylline.
NextBio537.
SOURCESearch...

Entry information

Entry nameAA1R_HUMAN
AccessionPrimary (citable) accession number: P30542
Secondary accession number(s): A6NFY5 expand/collapse secondary AC list , A6NGP4, A8K1L3, B3KXQ4, Q6FHK3, Q8TAM8
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: January 25, 2012
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents