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P30539

- GLGB_BUTFI

UniProt

P30539 - GLGB_BUTFI

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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Butyrivibrio fibrisolvens
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.By similarity

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei313 – 3131NucleophileBy similarity
Active sitei366 – 3661Proton donorBy similarity

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: CACAO
  2. cation binding Source: InterPro
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. glycogen biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgB (EC:2.4.1.18)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name:
BE
Gene namesi
Name:glgB
OrganismiButyrivibrio fibrisolvens
Taxonomic identifieri831 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesLachnospiraceaeButyrivibrio

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6396391,4-alpha-glucan branching enzyme GlgBPRO_0000188690Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP30539.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

P30539-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSQKVFISED DEYLFGQGTH YDIYDKLGAH PSEEKGKKGF FFAVWAPNAA
60 70 80 90 100
DVHVVGDFNG WDENAHQMKR SKTGNIWTLF IPGVAIGALY KFLITAQDGR
110 120 130 140 150
KLYKADPYAN YAELRPGNAS RTTDLSGFKW SDSKWYESLK GKDMNRQPIA
160 170 180 190 200
IYECHIGSWM KHPDGTEDGF YTYRQFADRI VEYLKEMKYT HIELIGIAEH
210 220 230 240 250
PFDGSWGYQV TGYYAPTARY GEPTDFMYLI NQLHKHGIGV ILDWVPAHFC
260 270 280 290 300
PDEFGLACFD GTCIYEDPDP RKGEHPDWGT KIFNLAKPEV KNFLIANALY
310 320 330 340 350
WIRKFHIDGL RVDAVASMLY LDYGKKDGQW VPNKYGDNKN LDAIEFFKHF
360 370 380 390 400
NSVVRGTYPN ILTIAEESTA WPKVTAPPEE DGLGFAFKWN MGWMHDFCEY
410 420 430 440 450
MKLDPYFRQG AHYMMTFAMS YNDSENYILP LSHDEVVHLK CSMVEKMPGY
460 470 480 490 500
KVDKYANLRV GYTYMFGHSG KKLLFMGQDF GQEREWSEKR ELDWFLLEND
510 520 530 540 550
LNRGMKDYVG KLLEIYRKYP ALYEVDNDWG GFEWINADDK ERSTYSFYRR
560 570 580 590 600
ASNGKDNILF VLNMTPMERK GFKVGVPFDG TYTKILDSAK ECYGGSGSSV
610 620 630
PDKIKAVKGL CDYKDYSIEF DLPPYGAEVF VFQTKKTKN
Length:639
Mass (Da):73,876
Last modified:April 1, 1993 - v1
Checksum:iDC93B72A5F28C2AF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64980 Genomic DNA. Translation: AAA23007.1.
PIRiB41328.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64980 Genomic DNA. Translation: AAA23007.1 .
PIRi B41328.

3D structure databases

ProteinModelPortali P30539.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi CBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00164 .

Family and domain databases

Gene3Di 2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_00685. GlgB.
InterProi IPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
[Graphical view ]
PANTHERi PTHR10357. PTHR10357. 1 hit.
Pfami PF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view ]
PIRSFi PIRSF000463. GlgB. 1 hit.
SUPFAMi SSF51445. SSF51445. 1 hit.
TIGRFAMsi TIGR01515. branching_enzym. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Characterization of the Butyrivibrio fibrisolvens glgB gene, which encodes a glycogen-branching enzyme with starch-clearing activity."
    Rumbak E., Rawlings D.E., Lindsey G.G., Woods D.R.
    J. Bacteriol. 173:6732-6741(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-12.
    Strain: H17C.

Entry informationi

Entry nameiGLGB_BUTFI
AccessioniPrimary (citable) accession number: P30539
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: October 1, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3