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Reviewed, UniProtKB/Swiss-Prot P30539 (GLGB_BUTFI)

Last modified February 9, 2010. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    1,4-alpha-glucan-branching enzyme
    EC=2.4.1.18
Alternative name(s):
    Glycogen-branching enzyme
      Short name=BE
    1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Gene names
Name: glgB
OrganismButyrivibrio fibrisolvens
Taxonomic identifier831 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Clostridia › Clostridiales › Lachnospiraceae › Butyrivibrio

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Protein attributes

Sequence length639 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position. Transfers chains of 5 to 10 (optimum, 7) glucose units, using amylose and amylopectin as substrates, to produce a highly branched polymer. HAMAP MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685

Subunit structure

Monomer By similarity. HAMAP MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 6396391,4-alpha-glucan-branching enzyme HAMAP MF_00685
PRO_0000188690

Sites

Active site2081 By similarity
Active site2431 By similarity
Active site2481 By similarity
Active site3111 By similarity
Active site3131 By similarity
Active site3661 By similarity
Active site4331 By similarity
Active site4341 By similarity

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Sequences

Sequence LengthMass (Da)Tools
P30539-1 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: DC93B72A5F28C2AF

FASTA63973,876
        10         20         30         40         50         60 
MSQKVFISED DEYLFGQGTH YDIYDKLGAH PSEEKGKKGF FFAVWAPNAA DVHVVGDFNG 

        70         80         90        100        110        120 
WDENAHQMKR SKTGNIWTLF IPGVAIGALY KFLITAQDGR KLYKADPYAN YAELRPGNAS 

       130        140        150        160        170        180 
RTTDLSGFKW SDSKWYESLK GKDMNRQPIA IYECHIGSWM KHPDGTEDGF YTYRQFADRI 

       190        200        210        220        230        240 
VEYLKEMKYT HIELIGIAEH PFDGSWGYQV TGYYAPTARY GEPTDFMYLI NQLHKHGIGV 

       250        260        270        280        290        300 
ILDWVPAHFC PDEFGLACFD GTCIYEDPDP RKGEHPDWGT KIFNLAKPEV KNFLIANALY 

       310        320        330        340        350        360 
WIRKFHIDGL RVDAVASMLY LDYGKKDGQW VPNKYGDNKN LDAIEFFKHF NSVVRGTYPN 

       370        380        390        400        410        420 
ILTIAEESTA WPKVTAPPEE DGLGFAFKWN MGWMHDFCEY MKLDPYFRQG AHYMMTFAMS 

       430        440        450        460        470        480 
YNDSENYILP LSHDEVVHLK CSMVEKMPGY KVDKYANLRV GYTYMFGHSG KKLLFMGQDF 

       490        500        510        520        530        540 
GQEREWSEKR ELDWFLLEND LNRGMKDYVG KLLEIYRKYP ALYEVDNDWG GFEWINADDK 

       550        560        570        580        590        600 
ERSTYSFYRR ASNGKDNILF VLNMTPMERK GFKVGVPFDG TYTKILDSAK ECYGGSGSSV 

       610        620        630 
PDKIKAVKGL CDYKDYSIEF DLPPYGAEVF VFQTKKTKN

« Hide

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References

[1]"Characterization of the Butyrivibrio fibrisolvens glgB gene, which encodes a glycogen-branching enzyme with starch-clearing activity."
Rumbak E., Rawlings D.E., Lindsey G.G., Woods D.R.
J. Bacteriol. 173:6732-6741(1991) [PubMed: 1938880] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-12.
Strain: H17C.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M64980 Genomic DNA. Translation: AAA23007.1.
PIRB41328.

3D structure databases

ModBaseSearch...

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Enzyme and pathway databases

BRENDA2.4.1.18. 1431.

Family and domain databases

HAMAPMF_00685. GlgB.
[Tree]
InterProIPR006407. 1-4-A-glucan_branch_enz_core.
IPR006048. A-amylase_b_C.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR017853. Glyco_hydro_catalytic_core.
IPR013781. Glyco_hydro_sg_catalytic.
IPR013783. Ig-like_fold.
[Graphical view]
Gene3DG3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit.
G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

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Entry information

Entry nameGLGB_BUTFI
AccessionPrimary (citable) accession number: P30539
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: February 9, 2010
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents