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P30539 (GLGB_BUTFI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
OrganismButyrivibrio fibrisolvens
Taxonomic identifier831 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaClostridialesLachnospiraceaeButyrivibrio

Protein attributes

Sequence length639 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 6396391,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_0000188690

Sites

Active site3131Nucleophile By similarity
Active site3661Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
P30539 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: DC93B72A5F28C2AF

FASTA63973,876
        10         20         30         40         50         60 
MSQKVFISED DEYLFGQGTH YDIYDKLGAH PSEEKGKKGF FFAVWAPNAA DVHVVGDFNG 

        70         80         90        100        110        120 
WDENAHQMKR SKTGNIWTLF IPGVAIGALY KFLITAQDGR KLYKADPYAN YAELRPGNAS 

       130        140        150        160        170        180 
RTTDLSGFKW SDSKWYESLK GKDMNRQPIA IYECHIGSWM KHPDGTEDGF YTYRQFADRI 

       190        200        210        220        230        240 
VEYLKEMKYT HIELIGIAEH PFDGSWGYQV TGYYAPTARY GEPTDFMYLI NQLHKHGIGV 

       250        260        270        280        290        300 
ILDWVPAHFC PDEFGLACFD GTCIYEDPDP RKGEHPDWGT KIFNLAKPEV KNFLIANALY 

       310        320        330        340        350        360 
WIRKFHIDGL RVDAVASMLY LDYGKKDGQW VPNKYGDNKN LDAIEFFKHF NSVVRGTYPN 

       370        380        390        400        410        420 
ILTIAEESTA WPKVTAPPEE DGLGFAFKWN MGWMHDFCEY MKLDPYFRQG AHYMMTFAMS 

       430        440        450        460        470        480 
YNDSENYILP LSHDEVVHLK CSMVEKMPGY KVDKYANLRV GYTYMFGHSG KKLLFMGQDF 

       490        500        510        520        530        540 
GQEREWSEKR ELDWFLLEND LNRGMKDYVG KLLEIYRKYP ALYEVDNDWG GFEWINADDK 

       550        560        570        580        590        600 
ERSTYSFYRR ASNGKDNILF VLNMTPMERK GFKVGVPFDG TYTKILDSAK ECYGGSGSSV 

       610        620        630 
PDKIKAVKGL CDYKDYSIEF DLPPYGAEVF VFQTKKTKN 

« Hide

References

[1]"Characterization of the Butyrivibrio fibrisolvens glgB gene, which encodes a glycogen-branching enzyme with starch-clearing activity."
Rumbak E., Rawlings D.E., Lindsey G.G., Woods D.R.
J. Bacteriol. 173:6732-6741(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-12.
Strain: H17C.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M64980 Genomic DNA. Translation: AAA23007.1.
PIRB41328.

3D structure databases

ProteinModelPortalP30539.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_BUTFI
AccessionPrimary (citable) accession number: P30539
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: June 11, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries