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P30539

- GLGB_BUTFI

UniProt

P30539 - GLGB_BUTFI

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Protein
1,4-alpha-glucan branching enzyme GlgB
Gene
glgB
Organism
Butyrivibrio fibrisolvens
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei313 – 3131Nucleophile By similarity
Active sitei366 – 3661Proton donor By similarity

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: CACAO
  2. cation binding Source: InterPro
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. glycogen biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgB (EC:2.4.1.18)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name:
BE
Gene namesi
Name:glgB
OrganismiButyrivibrio fibrisolvens
Taxonomic identifieri831 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaClostridialesLachnospiraceaeButyrivibrio

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6396391,4-alpha-glucan branching enzyme GlgBUniRule annotation
PRO_0000188690Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP30539.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

P30539-1 [UniParc]FASTAAdd to Basket

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MSQKVFISED DEYLFGQGTH YDIYDKLGAH PSEEKGKKGF FFAVWAPNAA    50
DVHVVGDFNG WDENAHQMKR SKTGNIWTLF IPGVAIGALY KFLITAQDGR 100
KLYKADPYAN YAELRPGNAS RTTDLSGFKW SDSKWYESLK GKDMNRQPIA 150
IYECHIGSWM KHPDGTEDGF YTYRQFADRI VEYLKEMKYT HIELIGIAEH 200
PFDGSWGYQV TGYYAPTARY GEPTDFMYLI NQLHKHGIGV ILDWVPAHFC 250
PDEFGLACFD GTCIYEDPDP RKGEHPDWGT KIFNLAKPEV KNFLIANALY 300
WIRKFHIDGL RVDAVASMLY LDYGKKDGQW VPNKYGDNKN LDAIEFFKHF 350
NSVVRGTYPN ILTIAEESTA WPKVTAPPEE DGLGFAFKWN MGWMHDFCEY 400
MKLDPYFRQG AHYMMTFAMS YNDSENYILP LSHDEVVHLK CSMVEKMPGY 450
KVDKYANLRV GYTYMFGHSG KKLLFMGQDF GQEREWSEKR ELDWFLLEND 500
LNRGMKDYVG KLLEIYRKYP ALYEVDNDWG GFEWINADDK ERSTYSFYRR 550
ASNGKDNILF VLNMTPMERK GFKVGVPFDG TYTKILDSAK ECYGGSGSSV 600
PDKIKAVKGL CDYKDYSIEF DLPPYGAEVF VFQTKKTKN 639
Length:639
Mass (Da):73,876
Last modified:April 1, 1993 - v1
Checksum:iDC93B72A5F28C2AF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M64980 Genomic DNA. Translation: AAA23007.1.
PIRiB41328.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M64980 Genomic DNA. Translation: AAA23007.1 .
PIRi B41328.

3D structure databases

ProteinModelPortali P30539.
ModBasei Search...

Protein family/group databases

CAZyi CBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00164 .

Family and domain databases

Gene3Di 2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_00685. GlgB.
InterProi IPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
[Graphical view ]
PANTHERi PTHR10357. PTHR10357. 1 hit.
Pfami PF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view ]
PIRSFi PIRSF000463. GlgB. 1 hit.
SUPFAMi SSF51445. SSF51445. 1 hit.
TIGRFAMsi TIGR01515. branching_enzym. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Characterization of the Butyrivibrio fibrisolvens glgB gene, which encodes a glycogen-branching enzyme with starch-clearing activity."
    Rumbak E., Rawlings D.E., Lindsey G.G., Woods D.R.
    J. Bacteriol. 173:6732-6741(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-12.
    Strain: H17C.

Entry informationi

Entry nameiGLGB_BUTFI
AccessioniPrimary (citable) accession number: P30539
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: June 11, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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