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P30538 (GLGB_GEOSE) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
OrganismGeobacillus stearothermophilus (Bacillus stearothermophilus)
Taxonomic identifier1422 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

Protein attributes

Sequence length639 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Biophysicochemical properties

Temperature dependence:

Optimum temperature is about 55 degrees Celsius. HAMAP-Rule MF_00685

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 6396391,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_0000188682

Sites

Active site3091Nucleophile By similarity
Active site3521Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
P30538 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: F5BD4446B371E03A

FASTA63974,795
        10         20         30         40         50         60 
MIAVGPTDLE IYLFHEGSLY KSYELFGAHV IKKNGMVGTR FCVWAPHARE VRLVGSFNEW 

        70         80         90        100        110        120 
NGTNFNLMKV SNQGVWMIFI PENLEGHLYK YEITTNDGNV LLKSDPYAFY SELRPHTASI 

       130        140        150        160        170        180 
VYNIKGYQWN DQTWRRKKQR KRIYDQPLFI YELHFGSWKK KEDGSFYTYQ EMAEELIPYV 

       190        200        210        220        230        240 
LEHGFTHIEL LPLVEHPFDR SWGYQGIGYY SATSRYGTPH DLMYFIDRCH QAGIGVILDW 

       250        260        270        280        290        300 
VPGHFCKDSH GLYMFDGAPA YEYANMQDRE NYVWGTANFD LGKPEVRSFL ISNALFWMEY 

       310        320        330        340        350        360 
FHVDGFRVDA VANMLYWPNS DVLYKNTYAV EFLQKLNETV FAYDPNILMI AEDSTDWPRV 

       370        380        390        400        410        420 
TAPTYDGGLG FNYKWNMGWM NDILTYMETP PEHRKYVHNK VTFSLLYAYS ENFILPFSHD 

       430        440        450        460        470        480 
EVVHGKKSLL SKMPGTYEEK FAQLRLLYGY LLTHPGKKLL FMGGEFGQFD EWKDLEQLDW 

       490        500        510        520        530        540 
MLFDFDMHRN MNMYVKELLK CYKRYKPLYE LDHSPDGFEW IDVHNAEQSI FSFIRRGKKE 

       550        560        570        580        590        600 
DDLLIVVCNF TNKVYHGYKV GVPLFTRYRE VINSDAIQFG GFGNINPKPI AAMEGPFHGK 

       610        620        630 
PYHIQMTIPP FGISILRPVK KGSVKSFMKT PHPPSHGAS 

« Hide

References

[1]"Molecular cloning and nucleotide sequence of the glycogen branching enzyme gene (glgB) from Bacillus stearothermophilus and expression in Escherichia coli and Bacillus subtilis."
Kiel J.A.K.W., Boels J.M., Beldman G., Venema G.
Mol. Gen. Genet. 230:136-144(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 1503-4R.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M35089 Genomic DNA. Translation: AAA22482.1.
PIRS18599.

3D structure databases

ProteinModelPortalP30538.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_GEOSE
AccessionPrimary (citable) accession number: P30538
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: June 11, 2014
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries