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P30538

- GLGB_GEOSE

UniProt

P30538 - GLGB_GEOSE

Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (01 Apr 1993)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.By similarity

    Catalytic activityi

    Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

    Temperature dependencei

    Optimum temperature is about 55 degrees Celsius.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei309 – 3091NucleophileBy similarity
    Active sitei352 – 3521Proton donorBy similarity

    GO - Molecular functioni

    1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-HAMAP
    2. cation binding Source: InterPro
    3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

    GO - Biological processi

    1. glycogen biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

    Enzyme and pathway databases

    UniPathwayiUPA00164.

    Protein family/group databases

    CAZyiCBM48. Carbohydrate-Binding Module Family 48.
    GH13. Glycoside Hydrolase Family 13.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1,4-alpha-glucan branching enzyme GlgB (EC:2.4.1.18)
    Alternative name(s):
    1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
    Alpha-(1->4)-glucan branching enzyme
    Glycogen branching enzyme
    Short name:
    BE
    Gene namesi
    Name:glgB
    OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)
    Taxonomic identifieri1422 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 6396391,4-alpha-glucan branching enzyme GlgBPRO_0000188682Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP30538.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di2.60.40.10. 1 hit.
    2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPiMF_00685. GlgB.
    InterProiIPR006048. A-amylase_b_C.
    IPR006407. GlgB.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR004193. Glyco_hydro_13_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    [Graphical view]
    PANTHERiPTHR10357. PTHR10357. 1 hit.
    PfamiPF00128. Alpha-amylase. 1 hit.
    PF02806. Alpha-amylase_C. 1 hit.
    PF02922. CBM_48. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000463. GlgB. 1 hit.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF81296. SSF81296. 1 hit.
    TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P30538-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIAVGPTDLE IYLFHEGSLY KSYELFGAHV IKKNGMVGTR FCVWAPHARE    50
    VRLVGSFNEW NGTNFNLMKV SNQGVWMIFI PENLEGHLYK YEITTNDGNV 100
    LLKSDPYAFY SELRPHTASI VYNIKGYQWN DQTWRRKKQR KRIYDQPLFI 150
    YELHFGSWKK KEDGSFYTYQ EMAEELIPYV LEHGFTHIEL LPLVEHPFDR 200
    SWGYQGIGYY SATSRYGTPH DLMYFIDRCH QAGIGVILDW VPGHFCKDSH 250
    GLYMFDGAPA YEYANMQDRE NYVWGTANFD LGKPEVRSFL ISNALFWMEY 300
    FHVDGFRVDA VANMLYWPNS DVLYKNTYAV EFLQKLNETV FAYDPNILMI 350
    AEDSTDWPRV TAPTYDGGLG FNYKWNMGWM NDILTYMETP PEHRKYVHNK 400
    VTFSLLYAYS ENFILPFSHD EVVHGKKSLL SKMPGTYEEK FAQLRLLYGY 450
    LLTHPGKKLL FMGGEFGQFD EWKDLEQLDW MLFDFDMHRN MNMYVKELLK 500
    CYKRYKPLYE LDHSPDGFEW IDVHNAEQSI FSFIRRGKKE DDLLIVVCNF 550
    TNKVYHGYKV GVPLFTRYRE VINSDAIQFG GFGNINPKPI AAMEGPFHGK 600
    PYHIQMTIPP FGISILRPVK KGSVKSFMKT PHPPSHGAS 639
    Length:639
    Mass (Da):74,795
    Last modified:April 1, 1993 - v1
    Checksum:iF5BD4446B371E03A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M35089 Genomic DNA. Translation: AAA22482.1.
    PIRiS18599.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M35089 Genomic DNA. Translation: AAA22482.1 .
    PIRi S18599.

    3D structure databases

    ProteinModelPortali P30538.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi CBM48. Carbohydrate-Binding Module Family 48.
    GH13. Glycoside Hydrolase Family 13.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00164 .

    Family and domain databases

    Gene3Di 2.60.40.10. 1 hit.
    2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPi MF_00685. GlgB.
    InterProi IPR006048. A-amylase_b_C.
    IPR006407. GlgB.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR004193. Glyco_hydro_13_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    [Graphical view ]
    PANTHERi PTHR10357. PTHR10357. 1 hit.
    Pfami PF00128. Alpha-amylase. 1 hit.
    PF02806. Alpha-amylase_C. 1 hit.
    PF02922. CBM_48. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000463. GlgB. 1 hit.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    SSF81296. SSF81296. 1 hit.
    TIGRFAMsi TIGR01515. branching_enzym. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and nucleotide sequence of the glycogen branching enzyme gene (glgB) from Bacillus stearothermophilus and expression in Escherichia coli and Bacillus subtilis."
      Kiel J.A.K.W., Boels J.M., Beldman G., Venema G.
      Mol. Gen. Genet. 230:136-144(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 1503-4R.

    Entry informationi

    Entry nameiGLGB_GEOSE
    AccessioniPrimary (citable) accession number: P30538
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: April 1, 1993
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3