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P30538

- GLGB_GEOSE

UniProt

P30538 - GLGB_GEOSE

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Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.By similarity

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

Temperature dependencei

Optimum temperature is about 55 degrees Celsius.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei309 – 3091NucleophileBy similarity
Active sitei352 – 3521Proton donorBy similarity

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-HAMAP
  2. cation binding Source: InterPro
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. glycogen biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgB (EC:2.4.1.18)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name:
BE
Gene namesi
Name:glgB
OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)
Taxonomic identifieri1422 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 6396391,4-alpha-glucan branching enzyme GlgBPRO_0000188682Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP30538.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

P30538-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIAVGPTDLE IYLFHEGSLY KSYELFGAHV IKKNGMVGTR FCVWAPHARE
60 70 80 90 100
VRLVGSFNEW NGTNFNLMKV SNQGVWMIFI PENLEGHLYK YEITTNDGNV
110 120 130 140 150
LLKSDPYAFY SELRPHTASI VYNIKGYQWN DQTWRRKKQR KRIYDQPLFI
160 170 180 190 200
YELHFGSWKK KEDGSFYTYQ EMAEELIPYV LEHGFTHIEL LPLVEHPFDR
210 220 230 240 250
SWGYQGIGYY SATSRYGTPH DLMYFIDRCH QAGIGVILDW VPGHFCKDSH
260 270 280 290 300
GLYMFDGAPA YEYANMQDRE NYVWGTANFD LGKPEVRSFL ISNALFWMEY
310 320 330 340 350
FHVDGFRVDA VANMLYWPNS DVLYKNTYAV EFLQKLNETV FAYDPNILMI
360 370 380 390 400
AEDSTDWPRV TAPTYDGGLG FNYKWNMGWM NDILTYMETP PEHRKYVHNK
410 420 430 440 450
VTFSLLYAYS ENFILPFSHD EVVHGKKSLL SKMPGTYEEK FAQLRLLYGY
460 470 480 490 500
LLTHPGKKLL FMGGEFGQFD EWKDLEQLDW MLFDFDMHRN MNMYVKELLK
510 520 530 540 550
CYKRYKPLYE LDHSPDGFEW IDVHNAEQSI FSFIRRGKKE DDLLIVVCNF
560 570 580 590 600
TNKVYHGYKV GVPLFTRYRE VINSDAIQFG GFGNINPKPI AAMEGPFHGK
610 620 630
PYHIQMTIPP FGISILRPVK KGSVKSFMKT PHPPSHGAS
Length:639
Mass (Da):74,795
Last modified:April 1, 1993 - v1
Checksum:iF5BD4446B371E03A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35089 Genomic DNA. Translation: AAA22482.1.
PIRiS18599.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35089 Genomic DNA. Translation: AAA22482.1 .
PIRi S18599.

3D structure databases

ProteinModelPortali P30538.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi CBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00164 .

Family and domain databases

Gene3Di 2.60.40.10. 1 hit.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPi MF_00685. GlgB.
InterProi IPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view ]
PANTHERi PTHR10357. PTHR10357. 1 hit.
Pfami PF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view ]
PIRSFi PIRSF000463. GlgB. 1 hit.
SUPFAMi SSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsi TIGR01515. branching_enzym. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning and nucleotide sequence of the glycogen branching enzyme gene (glgB) from Bacillus stearothermophilus and expression in Escherichia coli and Bacillus subtilis."
    Kiel J.A.K.W., Boels J.M., Beldman G., Venema G.
    Mol. Gen. Genet. 230:136-144(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 1503-4R.

Entry informationi

Entry nameiGLGB_GEOSE
AccessioniPrimary (citable) accession number: P30538
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: October 1, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3