Reviewed, UniProtKB/Swiss-Prot P30538 (GLGB_BACST)

Names and origin

Protein names

Recommended name:
    1,4-alpha-glucan-branching enzyme
    EC=2.4.1.18
Alternative name(s):
    Glycogen-branching enzyme
      Short name=BE
    1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase

Gene names

Name:

glgB

Organism

Bacillus stearothermophilus (Geobacillus stearothermophilus)

Taxonomic identifier

1422 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	639 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity.
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.
Biophysicochemical properties	Temperature dependence: Optimum temperature is about 55 degrees Celsius. HAMAP MF_00685

Ontologies

Keywords
Biological process	Glycogen biosynthesis
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 639	639	1,4-alpha-glucan-branching enzyme HAMAP MF_00685		PRO_0000188682
Sites
	Active site	204	1	By similarity
	Active site	239	1	By similarity
	Active site	244	1	By similarity
	Active site	307	1	By similarity
	Active site	309	1	By similarity
	Active site	352	1	By similarity
	Active site	419	1	By similarity
	Active site	420	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P30538-1 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: F5BD4446B371E03A	FASTA	639	74,795
	10 20 30 40 50 60 MIAVGPTDLE IYLFHEGSLY KSYELFGAHV IKKNGMVGTR FCVWAPHARE VRLVGSFNEW 70 80 90 100 110 120 NGTNFNLMKV SNQGVWMIFI PENLEGHLYK YEITTNDGNV LLKSDPYAFY SELRPHTASI 130 140 150 160 170 180 VYNIKGYQWN DQTWRRKKQR KRIYDQPLFI YELHFGSWKK KEDGSFYTYQ EMAEELIPYV 190 200 210 220 230 240 LEHGFTHIEL LPLVEHPFDR SWGYQGIGYY SATSRYGTPH DLMYFIDRCH QAGIGVILDW 250 260 270 280 290 300 VPGHFCKDSH GLYMFDGAPA YEYANMQDRE NYVWGTANFD LGKPEVRSFL ISNALFWMEY 310 320 330 340 350 360 FHVDGFRVDA VANMLYWPNS DVLYKNTYAV EFLQKLNETV FAYDPNILMI AEDSTDWPRV 370 380 390 400 410 420 TAPTYDGGLG FNYKWNMGWM NDILTYMETP PEHRKYVHNK VTFSLLYAYS ENFILPFSHD 430 440 450 460 470 480 EVVHGKKSLL SKMPGTYEEK FAQLRLLYGY LLTHPGKKLL FMGGEFGQFD EWKDLEQLDW 490 500 510 520 530 540 MLFDFDMHRN MNMYVKELLK CYKRYKPLYE LDHSPDGFEW IDVHNAEQSI FSFIRRGKKE 550 560 570 580 590 600 DDLLIVVCNF TNKVYHGYKV GVPLFTRYRE VINSDAIQFG GFGNINPKPI AAMEGPFHGK 610 620 630 PYHIQMTIPP FGISILRPVK KGSVKSFMKT PHPPSHGAS

References

[1]

"Molecular cloning and nucleotide sequence of the glycogen branching enzyme gene (glgB) from Bacillus stearothermophilus and expression in Escherichia coli and Bacillus subtilis." Kiel J.A.K.W., Boels J.M., Beldman G., Venema G. Mol. Gen. Genet. 230:136-144(1991) [PubMed: 1745226] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 1503-4R.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M35089 Genomic DNA. Translation: AAA22482.1.
PIR	S18599.
3D structure databases
HSSP	HSSP built from PDB template 1M7X based on UniProtKB P07762.
ModBase	Search...
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Enzyme and pathway databases
BRENDA	2.4.1.18. 266715.
Family and domain databases
HAMAP	MF_00685. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz_core. IPR006048. A-amylase_b_C. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_sg_catalytic. IPR013783. Ig-like_fold. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
TIGRFAMs	TIGR01515. branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB_BACST

Accession

Primary (citable) accession number: P30538

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	June 16, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)