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P30537

- GLGB_BACCL

UniProt

P30537 - GLGB_BACCL

Protein

1,4-alpha-glucan branching enzyme GlgB

Gene

glgB

Organism
Bacillus caldolyticus
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (01 Apr 1993)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.By similarity

    Catalytic activityi

    Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.

    Temperature dependencei

    Optimum temperature is about 39 degrees Celsius.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei309 – 3091NucleophileBy similarity
    Active sitei352 – 3521Proton donorBy similarity

    GO - Molecular functioni

    1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-HAMAP
    2. cation binding Source: InterPro
    3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

    GO - Biological processi

    1. glycogen biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

    Enzyme and pathway databases

    UniPathwayiUPA00164.

    Protein family/group databases

    CAZyiCBM48. Carbohydrate-Binding Module Family 48.
    GH13. Glycoside Hydrolase Family 13.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1,4-alpha-glucan branching enzyme GlgB (EC:2.4.1.18)
    Alternative name(s):
    1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
    Alpha-(1->4)-glucan branching enzyme
    Glycogen branching enzyme
    Short name:
    BE
    Gene namesi
    Name:glgB
    OrganismiBacillus caldolyticus
    Taxonomic identifieri1394 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillusGeobacillus thermoleovorans group

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 6666661,4-alpha-glucan branching enzyme GlgBPRO_0000188677Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP30537.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di2.60.40.10. 1 hit.
    2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPiMF_00685. GlgB.
    InterProiIPR006048. A-amylase_b_C.
    IPR006407. GlgB.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR004193. Glyco_hydro_13_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    [Graphical view]
    PANTHERiPTHR10357. PTHR10357. 1 hit.
    PfamiPF00128. Alpha-amylase. 1 hit.
    PF02806. Alpha-amylase_C. 1 hit.
    PF02922. CBM_48. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000463. GlgB. 1 hit.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    SSF81296. SSF81296. 1 hit.
    TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P30537-1 [UniParc]FASTAAdd to Basket

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    MIAANPTDLE VYLFHEGRLY QSYELFGAHV IRGGGAVGTR FCVWAPHARE    50
    VRLVGSFNDW NGTNSPLTKV NDEGVWTIVV PENLEGHLYK YEIITPDGRV 100
    LLKADPYAFY SELRPHTASI VYDLKGYEWN DSPWQRKKRR KRIYDQPMVI 150
    YELHFGSWKK KPDGRFYTYR EMADELIPYV LERGFTHIEL LPLVEHPLDR 200
    SWGYQGTGYY SVTSRYGTPH DFMYFVDRCH QAGLGVIIDW VPGHFCKDAH 250
    GLYMFDGAPT YEYANEKDRE NYVWGTANFD LGKPEVRSFL ISNALFWLEY 300
    YHVDGFRVDA VANMLYWPNN DRLYENPYAV EFLRQLNEAV FAYDPNVWMI 350
    AEDSTDWPRV TAPTYDGGLG FNYKWNMGWM NDMLKYMETP PHERKYAHNQ 400
    VSFSLLYAYS ENFILPFSHD EVVHGKKSLL NKMPGSYEEK FAQLRLLYGY 450
    MMAHPGKKLL FMGSEFAQFD EWKFAEELDW VLFDFELHRK MDEYVKQLIA 500
    CYKRYKPFYE LDHDPRGFEW IDVHNAEQSI FSFIRRGKKE GDVLVIVCNF 550
    TNQAYDDYKV SVPLLAPYRE VLNSDAAEFG GSGHVNGKRL PAFSEPFHGK 600
    PYHVRMTIPP FGISILRPVQ KRGERKQNEE EVHRHVIGRR ARKPASLADE 650
    KHRETSRAVW GEVPDH 666
    Length:666
    Mass (Da):78,096
    Last modified:April 1, 1993 - v1
    Checksum:i1F64C31495BB1B6E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z14057 Genomic DNA. Translation: CAA78440.1.
    PIRiB56639.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z14057 Genomic DNA. Translation: CAA78440.1 .
    PIRi B56639.

    3D structure databases

    ProteinModelPortali P30537.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi CBM48. Carbohydrate-Binding Module Family 48.
    GH13. Glycoside Hydrolase Family 13.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00164 .

    Family and domain databases

    Gene3Di 2.60.40.10. 1 hit.
    2.60.40.1180. 1 hit.
    3.20.20.80. 1 hit.
    HAMAPi MF_00685. GlgB.
    InterProi IPR006048. A-amylase_b_C.
    IPR006407. GlgB.
    IPR015902. Glyco_hydro_13.
    IPR013780. Glyco_hydro_13_b.
    IPR006047. Glyco_hydro_13_cat_dom.
    IPR004193. Glyco_hydro_13_N.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR013783. Ig-like_fold.
    IPR014756. Ig_E-set.
    [Graphical view ]
    PANTHERi PTHR10357. PTHR10357. 1 hit.
    Pfami PF00128. Alpha-amylase. 1 hit.
    PF02806. Alpha-amylase_C. 1 hit.
    PF02922. CBM_48. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000463. GlgB. 1 hit.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    SSF81296. SSF81296. 1 hit.
    TIGRFAMsi TIGR01515. branching_enzym. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The glgB gene from the thermophile Bacillus caldolyticus encodes a thermolabile branching enzyme."
      Kiel J.A.K.W., Boels J.M., Beldman G., Venema G.
      DNA Seq. 3:221-232(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiGLGB_BACCL
    AccessioniPrimary (citable) accession number: P30537
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: April 1, 1993
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3