P30537 (GLGB_BACCL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1,4-alpha-glucan branching enzyme GlgB
EC=2.4.1.18

Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE

Gene names

Name:

glgB

Organism

Bacillus caldolyticus

Taxonomic identifier

1394 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	666 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity. HAMAP MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.
Biophysicochemical properties	Temperature dependence: Optimum temperature is about 39 degrees Celsius. HAMAP MF_00685

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen biosynthesis Glycogen metabolism
Molecular function	Glycosyltransferase Transferase
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 666	666	1,4-alpha-glucan branching enzyme GlgB HAMAP MF_00685		PRO_0000188677
Sites
	Active site	309	1	Nucleophile By similarity
	Active site	352	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P30537 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 1F64C31495BB1B6E	FASTA	666	78,096
	10 20 30 40 50 60 MIAANPTDLE VYLFHEGRLY QSYELFGAHV IRGGGAVGTR FCVWAPHARE VRLVGSFNDW 70 80 90 100 110 120 NGTNSPLTKV NDEGVWTIVV PENLEGHLYK YEIITPDGRV LLKADPYAFY SELRPHTASI 130 140 150 160 170 180 VYDLKGYEWN DSPWQRKKRR KRIYDQPMVI YELHFGSWKK KPDGRFYTYR EMADELIPYV 190 200 210 220 230 240 LERGFTHIEL LPLVEHPLDR SWGYQGTGYY SVTSRYGTPH DFMYFVDRCH QAGLGVIIDW 250 260 270 280 290 300 VPGHFCKDAH GLYMFDGAPT YEYANEKDRE NYVWGTANFD LGKPEVRSFL ISNALFWLEY 310 320 330 340 350 360 YHVDGFRVDA VANMLYWPNN DRLYENPYAV EFLRQLNEAV FAYDPNVWMI AEDSTDWPRV 370 380 390 400 410 420 TAPTYDGGLG FNYKWNMGWM NDMLKYMETP PHERKYAHNQ VSFSLLYAYS ENFILPFSHD 430 440 450 460 470 480 EVVHGKKSLL NKMPGSYEEK FAQLRLLYGY MMAHPGKKLL FMGSEFAQFD EWKFAEELDW 490 500 510 520 530 540 VLFDFELHRK MDEYVKQLIA CYKRYKPFYE LDHDPRGFEW IDVHNAEQSI FSFIRRGKKE 550 560 570 580 590 600 GDVLVIVCNF TNQAYDDYKV SVPLLAPYRE VLNSDAAEFG GSGHVNGKRL PAFSEPFHGK 610 620 630 640 650 660 PYHVRMTIPP FGISILRPVQ KRGERKQNEE EVHRHVIGRR ARKPASLADE KHRETSRAVW GEVPDH

References

[1]	"The glgB gene from the thermophile Bacillus caldolyticus encodes a thermolabile branching enzyme." Kiel J.A.K.W., Boels J.M., Beldman G., Venema G. DNA Seq. 3:221-232(1992) [PubMed: 1296817] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z14057 Genomic DNA. Translation: CAA78440.1.
PIR	B56639.
3D structure databases
ProteinModelPortal	P30537.
ModBase	Search...
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_00685. GlgB. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz. IPR006048. A-amylase_b_C. IPR015902. Alpha_amylase. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF13. PTHR10357:SF13. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF000463. GlgB. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
TIGRFAMs	TIGR01515. Branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB_BACCL

Accession

Primary (citable) accession number: P30537

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	November 16, 2011
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program