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P30536

- TSPOA_HUMAN

UniProt

P30536 - TSPOA_HUMAN

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Protein

Translocator protein

Gene

TSPO

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Can bind protoporphyrin IX and may play a role in the transport of porphyrins and heme By similarity. Promotes the transport of cholesterol across mitochondrial membranes and may play a role in lipid metabolism (PubMed:24814875), but its precise physiological role is controversial. It is apparently not required for steroid hormone biosynthesis. Was initially identified as peripheral-type benzodiazepine receptor; can also bind isoquinoline carboxamides(PubMed:1847678).By similarity2 Publications

GO - Molecular functioni

  1. benzodiazepine receptor activity Source: ProtInc
  2. cholesterol binding Source: HGNC

GO - Biological processi

  1. anion transport Source: HGNC
  2. apoptotic process Source: HGNC
  3. cell proliferation Source: HGNC
  4. heme biosynthetic process Source: HGNC
  5. lipid transport Source: UniProtKB-KW
  6. protein targeting to mitochondrion Source: ProtInc
  7. regulation of cholesterol transport Source: HGNC
  8. steroid metabolic process Source: HGNC
  9. synaptic transmission Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Lipid transport, Transport

Protein family/group databases

TCDBi9.A.24.1.1. the mitochondrial cholesterol/porphyrin uptake translocator protein (tspo) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Translocator protein
Alternative name(s):
Mitochondrial benzodiazepine receptor
PKBS
Peripheral-type benzodiazepine receptor
Short name:
PBR
Gene namesi
Name:TSPO
Synonyms:BZRP, MBR
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 22

Organism-specific databases

HGNCiHGNC:1158. TSPO.

Subcellular locationi

Mitochondrion membrane 1 Publication; Multi-pass membrane protein 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: HPA
  2. extracellular vesicular exosome Source: UniProt
  3. integral component of membrane Source: UniProtKB-KW
  4. intracellular membrane-bounded organelle Source: HPA
  5. mitochondrial outer membrane Source: ProtInc
  6. mitochondrion Source: HPA
  7. nuclear membrane Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA25473.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 169169Translocator proteinPRO_0000190997Add
BLAST

Proteomic databases

MaxQBiP30536.
PaxDbiP30536.
PRIDEiP30536.

Expressioni

Tissue specificityi

Found in many tissue types. Expressed at the highest levels under normal conditions in tissues that synthesize steroids.1 Publication

Gene expression databases

BgeeiP30536.
CleanExiHS_TSPO.
ExpressionAtlasiP30536. baseline and differential.
GenevestigatoriP30536.

Organism-specific databases

HPAiCAB011232.
HPA046260.

Interactioni

Subunit structurei

Interacts with BZRAP1 By similarity. May interact with STAR By similarity. Interacts with MOST-1.By similarity2 Publications

Protein-protein interaction databases

BioGridi107168. 3 interactions.

Structurei

3D structure databases

ProteinModelPortaliP30536.
SMRiP30536. Positions 1-169.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 55Mitochondrial intermembraneBy similarity
Topological domaini27 – 4620CytoplasmicBy similarityAdd
BLAST
Topological domaini68 – 7912Mitochondrial intermembraneBy similarityAdd
BLAST
Topological domaini101 – 1055CytoplasmicBy similarity
Topological domaini127 – 1348Mitochondrial intermembraneBy similarity
Topological domaini156 – 16914CytoplasmicBy similarityAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei6 – 2621Helical; Name=1By similarityAdd
BLAST
Transmembranei47 – 6721Helical; Name=2By similarityAdd
BLAST
Transmembranei80 – 10021Helical; Name=3By similarityAdd
BLAST
Transmembranei106 – 12621Helical; Name=4By similarityAdd
BLAST
Transmembranei135 – 15521Helical; Name=5By similarityAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the TspO/BZRP family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG3476.
GeneTreeiENSGT00390000012980.
HOGENOMiHOG000224659.
InParanoidiP30536.
KOiK05770.
OMAiALNYRIW.
OrthoDBiEOG7V7687.
PhylomeDBiP30536.
TreeFamiTF342852.

Family and domain databases

InterProiIPR004307. TspO_MBR.
[Graphical view]
PANTHERiPTHR10057. PTHR10057. 1 hit.
PfamiPF03073. TspO_MBR. 1 hit.
[Graphical view]
PIRSFiPIRSF005859. PBR. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P30536-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAPPWVPAMG FTLAPSLGCF VGSRFVHGEG LRWYAGLQKP SWHPPHWVLG
60 70 80 90 100
PVWGTLYSAM GYGSYLVWKE LGGFTEKAVV PLGLYTGQLA LNWAWPPIFF
110 120 130 140 150
GARQMGWALV DLLLVSGAAA ATTVAWYQVS PLAARLLYPY LAWLAFTTTL
160
NYCVWRDNHG WRGGRRLPE
Length:169
Mass (Da):18,828
Last modified:November 30, 2010 - v3
Checksum:i6DD741BF99AB89D9
GO
Isoform 2 (identifier: B1AH88-1) [UniParc]FASTAAdd to Basket

Also known as: PBR-S

The sequence of this isoform can be found in the external entry B1AH88.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: No experimental confirmation available. The relatively low levels of the corresponding mRNA suggest that it might represent errors of the splicing machinery. In addition, isoform 2 is derived from a different reading frame, compared to isoform 1 and lacks homolog support.

Length:102
Mass (Da):10,537
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti147 – 1471T → A Associated with higher levels of pregnenolone production by lymphomonocytes and with increased plasma levels of cholesterol-rich low density lipoprotein. 6 Publications
Corresponds to variant rs6971 [ dbSNP | Ensembl ].
VAR_013617
Natural varianti162 – 1621R → H.5 Publications
Corresponds to variant rs6972 [ dbSNP | Ensembl ].
VAR_013618
Natural varianti169 – 1691E → Q.1 Publication
Corresponds to variant rs9333342 [ dbSNP | Ensembl ].
VAR_018868

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M36035 mRNA. Translation: AAA03652.1.
U12421 Genomic DNA. Translation: AAA83252.1.
AY998017 mRNA. Translation: AAY45787.1.
BT006949 mRNA. Translation: AAP35595.1.
CR456409 mRNA. Translation: CAG30295.1.
AY383615 Genomic DNA. Translation: AAQ75703.1.
Z82214 Genomic DNA. Translation: CAB55884.1.
BC001110 mRNA. Translation: AAH01110.1.
CCDSiCCDS33661.1. [P30536-1]
PIRiI38724.
JE0149.
S14257.
RefSeqiNP_000705.2. NM_000714.5. [P30536-1]
NP_001243459.1. NM_001256530.1. [P30536-1]
NP_001243460.1. NM_001256531.1. [P30536-1]
UniGeneiHs.202.

Genome annotation databases

EnsembliENST00000329563; ENSP00000328973; ENSG00000100300. [P30536-1]
ENST00000337554; ENSP00000338004; ENSG00000100300. [P30536-1]
ENST00000396265; ENSP00000379563; ENSG00000100300. [P30536-1]
GeneIDi706.
KEGGihsa:706.
UCSCiuc003bdn.4. human. [P30536-1]

Polymorphism databases

DMDMi313104268.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M36035 mRNA. Translation: AAA03652.1 .
U12421 Genomic DNA. Translation: AAA83252.1 .
AY998017 mRNA. Translation: AAY45787.1 .
BT006949 mRNA. Translation: AAP35595.1 .
CR456409 mRNA. Translation: CAG30295.1 .
AY383615 Genomic DNA. Translation: AAQ75703.1 .
Z82214 Genomic DNA. Translation: CAB55884.1 .
BC001110 mRNA. Translation: AAH01110.1 .
CCDSi CCDS33661.1. [P30536-1 ]
PIRi I38724.
JE0149.
S14257.
RefSeqi NP_000705.2. NM_000714.5. [P30536-1 ]
NP_001243459.1. NM_001256530.1. [P30536-1 ]
NP_001243460.1. NM_001256531.1. [P30536-1 ]
UniGenei Hs.202.

3D structure databases

ProteinModelPortali P30536.
SMRi P30536. Positions 1-169.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 107168. 3 interactions.

Chemistry

BindingDBi P30536.
ChEMBLi CHEMBL5742.
DrugBanki DB01178. Chlormezanone.
DB01068. Clonazepam.
DB00628. Clorazepate.
DB00829. Diazepam.
DB00402. Eszopiclone.
DB01544. Flunitrazepam.
DB01587. Ketazolam.
DB00186. Lorazepam.
DB00231. Temazepam.
DB00897. Triazolam.
DB00962. Zaleplon.
DB01198. Zopiclone.

Protein family/group databases

TCDBi 9.A.24.1.1. the mitochondrial cholesterol/porphyrin uptake translocator protein (tspo) family.

Polymorphism databases

DMDMi 313104268.

Proteomic databases

MaxQBi P30536.
PaxDbi P30536.
PRIDEi P30536.

Protocols and materials databases

DNASUi 706.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000329563 ; ENSP00000328973 ; ENSG00000100300 . [P30536-1 ]
ENST00000337554 ; ENSP00000338004 ; ENSG00000100300 . [P30536-1 ]
ENST00000396265 ; ENSP00000379563 ; ENSG00000100300 . [P30536-1 ]
GeneIDi 706.
KEGGi hsa:706.
UCSCi uc003bdn.4. human. [P30536-1 ]

Organism-specific databases

CTDi 706.
GeneCardsi GC22P043547.
HGNCi HGNC:1158. TSPO.
HPAi CAB011232.
HPA046260.
MIMi 109610. gene.
neXtProti NX_P30536.
PharmGKBi PA25473.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG3476.
GeneTreei ENSGT00390000012980.
HOGENOMi HOG000224659.
InParanoidi P30536.
KOi K05770.
OMAi ALNYRIW.
OrthoDBi EOG7V7687.
PhylomeDBi P30536.
TreeFami TF342852.

Miscellaneous databases

ChiTaRSi TSPO. human.
GeneWikii Translocator_protein.
GenomeRNAii 706.
NextBioi 2874.
PROi P30536.
SOURCEi Search...

Gene expression databases

Bgeei P30536.
CleanExi HS_TSPO.
ExpressionAtlasi P30536. baseline and differential.
Genevestigatori P30536.

Family and domain databases

InterProi IPR004307. TspO_MBR.
[Graphical view ]
PANTHERi PTHR10057. PTHR10057. 1 hit.
Pfami PF03073. TspO_MBR. 1 hit.
[Graphical view ]
PIRSFi PIRSF005859. PBR. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and chromosomal localization of a human peripheral-type benzodiazepine receptor."
    Riond J., Mattei M.-G., Kaghad M., Dumont X., Guillemot J.-C., le Fur G., Caput D., Ferrara P.
    Eur. J. Biochem. 195:305-311(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANTS ALA-147 AND HIS-162, FUNCTION, SUBCELLULAR LOCATION.
  2. "Comparison of repetitive elements in the third intron of human and rodent mitochondrial benzodiazepine receptor-encoding genes."
    Yakovlev A.G., Ruffo M., Jurka J., Krueger K.E.
    Gene 155:201-205(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Peripheral benzodiazepine receptor: characterization in human T-lymphoma Jurkat cells."
    Costa B., Salvetti A., Rossi L., Spinetti F., Lena A., Chelli B., Rechichi M., Da Pozzo E., Gremigni V., Martini C.
    Mol. Pharmacol. 69:37-44(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  4. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS ALA-147 AND HIS-162.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANTS ALA-147 AND HIS-162.
  6. NIEHS SNPs program
    Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ALA-147 AND GLN-169.
  7. "The DNA sequence of human chromosome 22."
    Dunham I., Hunt A.R., Collins J.E., Bruskiewich R., Beare D.M., Clamp M., Smink L.J., Ainscough R., Almeida J.P., Babbage A.K., Bagguley C., Bailey J., Barlow K.F., Bates K.N., Beasley O.P., Bird C.P., Blakey S.E., Bridgeman A.M.
    , Buck D., Burgess J., Burrill W.D., Burton J., Carder C., Carter N.P., Chen Y., Clark G., Clegg S.M., Cobley V.E., Cole C.G., Collier R.E., Connor R., Conroy D., Corby N.R., Coville G.J., Cox A.V., Davis J., Dawson E., Dhami P.D., Dockree C., Dodsworth S.J., Durbin R.M., Ellington A.G., Evans K.L., Fey J.M., Fleming K., French L., Garner A.A., Gilbert J.G.R., Goward M.E., Grafham D.V., Griffiths M.N.D., Hall C., Hall R.E., Hall-Tamlyn G., Heathcott R.W., Ho S., Holmes S., Hunt S.E., Jones M.C., Kershaw J., Kimberley A.M., King A., Laird G.K., Langford C.F., Leversha M.A., Lloyd C., Lloyd D.M., Martyn I.D., Mashreghi-Mohammadi M., Matthews L.H., Mccann O.T., Mcclay J., Mclaren S., McMurray A.A., Milne S.A., Mortimore B.J., Odell C.N., Pavitt R., Pearce A.V., Pearson D., Phillimore B.J.C.T., Phillips S.H., Plumb R.W., Ramsay H., Ramsey Y., Rogers L., Ross M.T., Scott C.E., Sehra H.K., Skuce C.D., Smalley S., Smith M.L., Soderlund C., Spragon L., Steward C.A., Sulston J.E., Swann R.M., Vaudin M., Wall M., Wallis J.M., Whiteley M.N., Willey D.L., Williams L., Williams S.A., Williamson H., Wilmer T.E., Wilming L., Wright C.L., Hubbard T., Bentley D.R., Beck S., Rogers J., Shimizu N., Minoshima S., Kawasaki K., Sasaki T., Asakawa S., Kudoh J., Shintani A., Shibuya K., Yoshizaki Y., Aoki N., Mitsuyama S., Roe B.A., Chen F., Chu L., Crabtree J., Deschamps S., Do A., Do T., Dorman A., Fang F., Fu Y., Hu P., Hua A., Kenton S., Lai H., Lao H.I., Lewis J., Lewis S., Lin S.-P., Loh P., Malaj E., Nguyen T., Pan H., Phan S., Qi S., Qian Y., Ray L., Ren Q., Shaull S., Sloan D., Song L., Wang Q., Wang Y., Wang Z., White J., Willingham D., Wu H., Yao Z., Zhan M., Zhang G., Chissoe S., Murray J., Miller N., Minx P., Fulton R., Johnson D., Bemis G., Bentley D., Bradshaw H., Bourne S., Cordes M., Du Z., Fulton L., Goela D., Graves T., Hawkins J., Hinds K., Kemp K., Latreille P., Layman D., Ozersky P., Rohlfing T., Scheet P., Walker C., Wamsley A., Wohldmann P., Pepin K., Nelson J., Korf I., Bedell J.A., Hillier L.W., Mardis E., Waterston R., Wilson R., Emanuel B.S., Shaikh T., Kurahashi H., Saitta S., Budarf M.L., McDermid H.E., Johnson A., Wong A.C.C., Morrow B.E., Edelmann L., Kim U.J., Shizuya H., Simon M.I., Dumanski J.P., Peyrard M., Kedra D., Seroussi E., Fransson I., Tapia I., Bruder C.E., O'Brien K.P., Wilkinson P., Bodenteich A., Hartman K., Hu X., Khan A.S., Lane L., Tilahun Y., Wright H.
    Nature 402:489-495(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Lymph.
  9. "Cloning and characterization of PRAX-1. A new protein that specifically interacts with the peripheral benzodiazepine receptor."
    Galiegue S., Jbilo O., Combes T., Bribes E., Carayon P., Le Fur G., Casellas P.
    J. Biol. Chem. 274:2938-2952(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH BZRAP1.
    Tissue: Brain.
  10. "Translocator protein (18kDa): new nomenclature for the peripheral-type benzodiazepine receptor based on its structure and molecular function."
    Papadopoulos V., Baraldi M., Guilarte T.R., Knudsen T.B., Lacapere J.-J., Lindemann P., Norenberg M.D., Nutt D., Weizman A., Zhang M.-R., Gavish M.
    Trends Pharmacol. Sci. 27:402-409(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE.
  11. "Cellular expression, localization and interactions of the product of the human MOST-1 gene associated with breast and prostate cancers."
    Tan J.M.M., Chow V.T.K.
    Int. J. Oncol. 30:81-89(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MOST-1.
  12. "Regulation of translocator protein 18 kDa (TSPO) expression in health and disease states."
    Batarseh A., Papadopoulos V.
    Mol. Cell. Endocrinol. 327:1-12(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  13. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  14. "Targeting mitochondrial 18kDa translocator protein (TSPO) regulates macrophage cholesterol efflux and lipid phenotype."
    Taylor J.M., Allen A.M., Graham A.
    Clin. Sci. 127:603-613(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  15. "An association study between two missense variations of the benzodiazepine receptor (peripheral) gene and schizophrenia in a Japanese sample."
    Kurumaji A., Nomoto H., Yoshikawa T., Okubo Y., Toru M.
    J. Neural Transm. 107:491-500(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANTS ALA-147 AND HIS-162.
  16. "No association of two missense variations of the benzodiazepine receptor (peripheral) gene and mood disorders in a Japanese sample."
    Kurumaji A., Nomoto H., Yamada K., Yoshikawa T., Toru M.
    Am. J. Med. Genet. 105:172-175(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANTS ALA-147 AND HIS-162.
  17. "The spontaneous Ala147Thr amino acid substitution within the translocator protein influences pregnenolone production in lymphomonocytes of healthy individuals."
    Costa B., Pini S., Gabelloni P., Da Pozzo E., Abelli M., Lari L., Preve M., Lucacchini A., Cassano G.B., Martini C.
    Endocrinology 150:5438-5445(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION OF VARIANT ALA-147.

Entry informationi

Entry nameiTSPOA_HUMAN
AccessioniPrimary (citable) accession number: P30536
Secondary accession number(s): Q53Y59, Q6ICF9, Q96TF6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: November 30, 2010
Last modified: October 29, 2014
This is version 133 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3