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Reviewed, UniProtKB/Swiss-Prot P30530 (UFO_HUMAN)

Last modified June 16, 2009. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (7) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Tyrosine-protein kinase receptor UFO
    EC=2.7.10.1
Alternative name(s):
    AXL oncogene
Gene names
Name: AXL
Synonyms: UFO
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length894 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

May function as a signal transducer between specific cell types of mesodermal origin. In case of filovirus infection, seems to function as a cell entry factor. Ref.8

Catalytic activity

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subunit structure

Heterodimer and heterotetramer with GAS6. Ref.12

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue.

Involvement in disease

Has transforming potential in patients with chronic myeloproliferative disorder or chronic myelocytic leukemia.

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.

Contains 2 fibronectin type-III domains.

Contains 2 Ig-like C2-type (immunoglobulin-like) domains.

Contains 1 protein kinase domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Long (identifier: P30530-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Short (identifier: P30530-2)

The sequence of this isoform differs from the canonical sequence as follows:
     429-437: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 894869Tyrosine-protein kinase receptor UFO
PRO_0000024481

Regions

Topological domain26 – 451426Extracellular Potential
Transmembrane452 – 47221 Potential
Topological domain473 – 894422Cytoplasmic Potential
Domain27 – 128102Ig-like C2-type 1
Domain139 – 22284Ig-like C2-type 2
Domain225 – 332108Fibronectin type-III 1
Domain333 – 42795Fibronectin type-III 2
Domain536 – 807272Protein kinase
Nucleotide binding542 – 5509ATP By similarity
Region26 – 9267Interaction with SKP1 and GAS6

Sites

Active site6721Proton acceptor By similarity
Binding site5671ATP By similarity

Amino acid modifications

Modified residue1001Phosphoserine
Modified residue7021Phosphotyrosine
Modified residue7031Phosphotyrosine; by autocatalysis By similarity
Modified residue8751Phosphoserine
Modified residue8841Phosphoserine
Glycosylation431N-linked (GlcNAc...) Potential
Glycosylation1571N-linked (GlcNAc...) Potential
Glycosylation1981N-linked (GlcNAc...) Potential
Glycosylation3391N-linked (GlcNAc...) Potential
Glycosylation3451N-linked (GlcNAc...) Potential
Glycosylation4011N-linked (GlcNAc...) Potential
Disulfide bond56 ↔ 117 By similarity
Disulfide bond160 ↔ 205 By similarity

Natural variations

Alternative sequence429 – 4379Missing in isoform Short.
VSP_005017
Natural variant1121T → M
VAR_045596
Natural variant2661N → D: dbSNP rs7249222.
VAR_057990
Natural variant2951R → W in a lung neuroendocrine carcinoma sample; somatic mutation.
VAR_045597
Natural variant4991R → C in a gastric adenocarcinoma sample; somatic mutation.
VAR_041877
Natural variant5151S → G
VAR_041878

Experimental info

Mutagenesis631E → R: Slightly reduced affinity for GAS6.
Mutagenesis661E → R: Reduced affinity for GAS6.
Mutagenesis841T → R: Reduced affinity for GAS6.
Sequence conflict3031T → P in AAB20305. Ref.3
Sequence conflict3031T → P in CAA40338. Ref.3
Sequence conflict3381E → K in AAA61243. Ref.2
Sequence conflict4301Q → E in AAB20305. Ref.3
Sequence conflict4301Q → E in CAA40338. Ref.3
Sequence conflict6391D → G in AAB20305. Ref.3
Sequence conflict6391D → G in CAA40338. Ref.3
Sequence conflict6961K → I Ref.7
Sequence conflict7641Q → R in AAH32229. Ref.5

Secondary structure

....................................... 894
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform Long [UniParc].

Last modified June 16, 2009. Version 3.
Checksum: 2C2D3574EADA4116

FASTA89498,336
        10         20         30         40         50         60 
MAWRCPRMGR VPLAWCLALC GWACMAPRGT QAEESPFVGN PGNITGARGL TGTLRCQLQV 

        70         80         90        100        110        120 
QGEPPEVHWL RDGQILELAD STQTQVPLGE DEQDDWIVVS QLRITSLQLS DTGQYQCLVF 

       130        140        150        160        170        180 
LGHQTFVSQP GYVGLEGLPY FLEEPEDRTV AANTPFNLSC QAQGPPEPVD LLWLQDAVPL 

       190        200        210        220        230        240 
ATAPGHGPQR SLHVPGLNKT SSFSCEAHNA KGVTTSRTAT ITVLPQQPRN LHLVSRQPTE 

       250        260        270        280        290        300 
LEVAWTPGLS GIYPLTHCTL QAVLSNDGMG IQAGEPDPPE EPLTSQASVP PHQLRLGSLH 

       310        320        330        340        350        360 
PHTPYHIRVA CTSSQGPSSW THWLPVETPE GVPLGPPENI SATRNGSQAF VHWQEPRAPL 

       370        380        390        400        410        420 
QGTLLGYRLA YQGQDTPEVL MDIGLRQEVT LELQGDGSVS NLTVCVAAYT AAGDGPWSLP 

       430        440        450        460        470        480 
VPLEAWRPGQ AQPVHQLVKE PSTPAFSWPW WYVLLGAVVA AACVLILALF LVHRRKKETR 

       490        500        510        520        530        540 
YGEVFEPTVE RGELVVRYRV RKSYSRRTTE ATLNSLGISE ELKEKLRDVM VDRHKVALGK 

       550        560        570        580        590        600 
TLGEGEFGAV MEGQLNQDDS ILKVAVKTMK IAICTRSELE DFLSEAVCMK EFDHPNVMRL 

       610        620        630        640        650        660 
IGVCFQGSER ESFPAPVVIL PFMKHGDLHS FLLYSRLGDQ PVYLPTQMLV KFMADIASGM 

       670        680        690        700        710        720 
EYLSTKRFIH RDLAARNCML NENMSVCVAD FGLSKKIYNG DYYRQGRIAK MPVKWIAIES 

       730        740        750        760        770        780 
LADRVYTSKS DVWSFGVTMW EIATRGQTPY PGVENSEIYD YLRQGNRLKQ PADCLDGLYA 

       790        800        810        820        830        840 
LMSRCWELNP QDRPSFTELR EDLENTLKAL PPAQEPDEIL YVNMDEGGGY PEPPGAAGGA 

       850        860        870        880        890 
DPPTQPDPKD SCSCLTAAEV HPAGRYVLCP STTPSPAQPA DRGSPAAPGQ EDGA 

« Hide

Isoform Short.

Checksum: AFF0212BA8EAEAA1
Show »

FASTA88597,377

References

« Hide 'large scale' references
[1]"Putative tyrosine kinases expressed in K-562 human leukemia cells."
Partanen J., Maekelae T.P., Alitalo R., Lehvaeslaiho H., Alitalo K.
Proc. Natl. Acad. Sci. U.S.A. 87:8913-8917(1990) [PubMed: 2247464] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ASP-266.
[2]"AXL, a transforming gene isolated from primary human myeloid leukemia cells, encodes a novel receptor tyrosine kinase."
O'Bryan J.P., Frye R.A., Cogswell P.C., Neubauer A., Kitch B., Prokop C., Espinosa R., le Beau M.M., Earp H., Liu E.T.
Mol. Cell. Biol. 11:5016-5031(1991) [PubMed: 1656220] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT), VARIANT ASP-266.
Tissue: Fibroblast.
[3]"A novel putative tyrosine kinase receptor with oncogenic potential."
Janssen J.W.G., Schulz A.S., Steenvoorden A.C.M., Schmidberger M., Strehl S., Ambros P., Bartram C.R.
Oncogene 6:2113-2120(1991) [PubMed: 1834974] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG), VARIANT ASP-266.
[4]"The DNA sequence and biology of human chromosome 19."
Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. expand/collapse author list , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
Nature 428:529-535(2004) [PubMed: 15057824] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG), VARIANT ASP-266.
[6]"A survey of protein tyrosine kinase mRNAs expressed in normal human melanocytes."
Lee S.-T., Strunk K.M., Spritz R.A.
Oncogene 8:3403-3410(1993) [PubMed: 8247543] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 674-730.
[7]"Receptor tyrosine kinases expressed in metastatic colon cancer."
Craven R.J., Xu L.H., Weiner T.M., Fridell Y.-W., Dent G.A., Srivastava S., Varnum B., Liu E.T., Cance W.G.
Int. J. Cancer 60:791-797(1995) [PubMed: 7896447] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 677-730, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Tissue: Colon tumor.
[8]"Tyro3 family-mediated cell entry of Ebola and Marburg viruses."
Shimojima M., Takada A., Ebihara H., Neumann G., Fujioka K., Irimura T., Jones S., Feldmann H., Kawaoka Y.
J. Virol. 80:10109-10116(2006) [PubMed: 17005688] [Abstract]
Cited for: FUNCTION AS CELL ENTRY FACTOR IN FILOVIRUS INFECTION.
[9]"Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer."
Rikova K., Guo A., Zeng Q., Possemato A., Yu J., Haack H., Nardone J., Lee K., Reeves C., Li Y., Hu Y., Tan Z., Stokes M., Sullivan L., Mitchell J., Wetzel R., Macneill J., Ren J.M. expand/collapse author list , Yuan J., Bakalarski C.E., Villen J., Kornhauser J.M., Smith B., Li D., Zhou X., Gygi S.P., Gu T.-L., Polakiewicz R.D., Rush J., Comb M.J.
Cell 131:1190-1203(2007) [PubMed: 18083107] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-702 AND TYR-703, MASS SPECTROMETRY.
[10]"Automated phosphoproteome analysis for cultured cancer cells by two-dimensional nanoLC-MS using a calcined titania/C18 biphasic column."
Imami K., Sugiyama N., Kyono Y., Tomita M., Ishihama Y.
Anal. Sci. 24:161-166(2008) [PubMed: 18187866] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-100, MASS SPECTROMETRY.
[11]"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-875 AND SER-884, MASS SPECTROMETRY.
[12]"Structural basis for Gas6-Axl signalling."
Sasaki T., Knyazev P.G., Clout N.J., Cheburkin Y., Goehring W., Ullrich A., Timpl R., Hohenester E.
EMBO J. 25:80-87(2006) [PubMed: 16362042] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.3 ANGSTROMS) OF 33-227 IN COMPLEX WITH GAS6, MUTAGENESIS OF GLU-63; GLU-66 AND THR-84, SUBUNIT.
[13]"Patterns of somatic mutation in human cancer genomes."
Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. expand/collapse author list , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
Nature 446:153-158(2007) [PubMed: 17344846] [Abstract]
Cited for: VARIANTS [LARGE SCALE ANALYSIS] MET-112; TRP-295; CYS-499 AND GLY-515.
+Additional computationally mapped references.

Cross-references

Sequence databases

M76125 mRNA. Translation: AAA61243.1.
S65125 mRNA. Translation: AAB20305.1.
X57019 mRNA. Translation: CAA40338.1.
AC011510 Genomic DNA. No translation available.
BC032229 mRNA. Translation: AAH32229.1.
IPIIPI00296992.
IPI00397361.
PIRA41527.
UniGeneHs.590970

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2C5DX-ray3.30C/D33-227[»]
ModBaseSearch...

PTM databases

PhosphoSiteP30530.

Proteomic databases

PRIDEP30530.

Genome annotation databases

EnsemblENSG00000167601. Homo sapiens. [Contig view]

Organism-specific databases

GeneCardsGC19P046416.
HGNCHGNC:905. AXL.
MIM109135. gene.
PharmGKBPA25197.
GenAtlasSearch...

Phylogenomic databases

HOVERGENP30530.

Enzyme and pathway databases

BRENDA2.7.10.1. 247.

Gene expression databases

ArrayExpressP30530.
BgeeP30530.
CleanExHS_AXL.
GermOnlineENSG00000167601. Homo sapiens.

Family and domain databases

InterProIPR015775. Axl_RTKs.
IPR008957. Fibronectin_typ-III-like_fold.
IPR003961. FN_III.
IPR007110. Ig-like.
IPR013783. Ig-like_fold.
IPR003599. Ig_sub.
IPR013106. Ig_V-set.
IPR000719. Prot_kinase_core.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Tyr_pkinase.
IPR008266. Tyr_pkinase_AS.
[Graphical view]
Gene3DG3DSA:2.60.40.30. FN_III-like. 1 hit.
G3DSA:2.60.40.10. Ig-like_fold. 2 hits.
PANTHERPTHR23256:SF156. Axl_RTKs. 1 hit.
PfamPF00041. fn3. 2 hits.
PF07714. Pkinase_Tyr. 1 hit.
PF07686. V-set. 1 hit.
[Graphical view]
PRINTSPR00109. TYRKINASE.
ProDomPD000001. Prot_kinase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00060. FN3. 2 hits.
SM00409. IG. 2 hits.
SM00219. TyrKc. 1 hit.
[Graphical view]
PROSITEPS50853. FN3. 2 hits.
PS50835. IG_LIKE. 2 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

SOURCESearch...

Entry information

Entry nameUFO_HUMAN
AccessionPrimary (citable) accession number: P30530
Secondary accession number(s): Q8N5L2, Q9UD27
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: June 16, 2009
Last modified: June 16, 2009
This is version 103 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

Human chromosome 19

Human chromosome 19: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents