P30495 (1B56_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
HLA class I histocompatibility antigen, B-56 alpha chain

Alternative name(s):
Bw-22
Bw-56
MHC class I antigen B*56

Gene names

Name:	HLA-B
Synonyms:	HLAB

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the presentation of foreign antigens to the immune system.
Subunit structure	Heterodimer of an alpha chain and a beta chain (beta-2-microglobulin). Interacts with human herpesvirus 8 MIR1 protein By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein.
Post-translational modification	Polyubiquitinated in a post ER compartment by interaction with human herpesvirus 8 MIR1 protein. This targets the protein for rapid degradation via the ubiquitin system By similarity.
Polymorphism	The following alleles of B-56 are known: B*56:01, B*56:02, B*56:03 (B22N), B*56:04, B*56:05 (B56V1), B*56:06 and B*56:07. The sequence shown is that of B*56:01.
Sequence similarities	Belongs to the MHC class I family. Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

Ontologies

Keywords
Biological process	Host-virus interaction Immunity
Cellular component	MHC I Membrane
Coding sequence diversity	Polymorphism
Domain	Signal Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent Traceable author statement. Source: Reactome antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent Traceable author statement. Source: Reactome interferon-gamma-mediated signaling pathway Traceable author statement. Source: Reactome regulation of immune response Traceable author statement. Source: Reactome type I interferon-mediated signaling pathway Traceable author statement. Source: Reactome virus-host interaction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	ER to Golgi transport vesicle membrane Traceable author statement. Source: Reactome Golgi membrane Traceable author statement. Source: Reactome MHC class I protein complex Inferred from electronic annotation. Source: UniProtKB-KW early endosome membrane Traceable author statement. Source: Reactome integral to lumenal side of endoplasmic reticulum membrane Traceable author statement. Source: Reactome phagocytic vesicle membrane Traceable author statement. Source: Reactome plasma membrane Traceable author statement. Source: Reactome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24
	Chain	25 – 362	338	HLA class I histocompatibility antigen, B-56 alpha chain		PRO_0000018859
Regions
	Topological domain	25 – 308	284	Extracellular Potential
	Transmembrane	309 – 332	24	Helical; Potential
	Topological domain	333 – 362	30	Cytoplasmic Potential
	Domain	209 – 295	87	Ig-like C1-type
	Region	25 – 114	90	Alpha-1
	Region	115 – 206	92	Alpha-2
	Region	207 – 298	92	Alpha-3
	Region	299 – 308	10	Connecting peptide
Amino acid modifications
	Glycosylation	110	1	N-linked (GlcNAc...) By similarity
	Disulfide bond	125 ↔ 188		By similarity
	Disulfide bond	227 ↔ 283		By similarity
Natural variations
	Natural variant	65	1	A → T. Corresponds to variant rs1050529 [ dbSNP | Ensembl ].		VAR_056413
	Natural variant	69	1	E → T in allele B*56:06; requires 2 nucleotide substitutions.		VAR_016486
	Natural variant	87	1	N → D. Corresponds to variant rs1050570 [ dbSNP | Ensembl ].		VAR_056414
	Natural variant	87	1	N → K. Corresponds to variant rs1065386 [ dbSNP | Ensembl ].		VAR_059491
	Natural variant	97	1	T → A. Corresponds to variant rs1050393 [ dbSNP | Ensembl ].		VAR_056415
	Natural variant	98	1	D → Y. Corresponds to variant rs1131215 [ dbSNP | Ensembl ].		VAR_059492
	Natural variant	101	1	S → N in allele B*56:07. Corresponds to variant rs1050388 [ dbSNP | Ensembl ].		VAR_016487
	Natural variant	104 – 107	4	NLRG → TALR in allele B*56:07.		VAR_016488
	Natural variant	119	1	W → L in allele B*56:02, allele B*56:03 and allele B*56:04.		VAR_016489
	Natural variant	121	1	T → R in allele B*56:02, allele B*56:03 and allele B*56:04.		VAR_016490
	Natural variant	127	1	L → V in allele B*56:03, allele B*56:04, allele B*56:05 and allele B*56:06.		VAR_016491
	Natural variant	138	1	N → D in allele B*56:03.		VAR_016707
	Natural variant	140	1	L → S in allele B*56:03.		VAR_016708
	Natural variant	140	1	L → Y in allele B*56:05 and allele B*56:06; requires 2 nucleotide substitutions.		VAR_016492
	Natural variant	176	1	V → E in allele B*56:03, allele B*56:05 and allele B*56:06.		VAR_016493
	Natural variant	180	1	L → W in allele B*56:03.		VAR_016709
	Natural variant	195	1	Y → H in allele B*56:05 and allele B*56:06.		VAR_016494

Sequences

	Sequence		Length	Mass (Da)
	P30495 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: D442225B8A09D667	FASTA	362	40,478
	10 20 30 40 50 60 MRVTAPRTLL LLLWGALALT ETWAGSHSMR YFYTAMSRPG RGEPRFIAVG YVDDTQFVRF 70 80 90 100 110 120 DSDAASPREE PRAPWIEQEG PEYWDRNTQI YKAQAQTDRE SLRNLRGYYN QSEAGSHTWQ 130 140 150 160 170 180 TMYGCDLGPD GRLLRGHNQL AYDGKDYIAL NEDLSSWTAA DTAAQITQRK WEAARVAEQL 190 200 210 220 230 240 RAYLEGLCVE WLRRYLENGK ETLQRADPPK THVTHHPISD HEATLRCWAL GFYPAEITLT 250 260 270 280 290 300 WQRDGEDQTQ DTELVETRPA GDRTFQKWAA VVVPSGEEQR YTCHVQHEGL PKPLTLRWEP 310 320 330 340 350 360 SSQSTIPIVG IVAGLAVLAV VVIGAVVATV MCRRKSSGGK GGSYSQAASS DSAQGSDVSL TA

References

[1]	"HLA-Bw22: a family of molecules with identity to HLA-B7 in the alpha 1-helix." Hildebrand W.H., Madrigal J.A., Little A.-M., Parham P. J. Immunol. 148:1155-1162(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ALLELES B*56:01 AND B*56:02).
[2]	"Five HLA-B22 group alleles in Japanese." Bannai M., Tokunaga K., Tanaka H., Lin L., Kashiwase K., Tokunaga K., Juji T. Tissue Antigens 49:376-382(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ALLELE B*56:03). Tissue: Blood.
[3]	"Novel HLA-B alleles." Steiner N.K., Lee K.W., Hurley C.K. Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE OF 26-206 (ALLELE B*56:04).
[4]	"HLA-B56V1(B*5605)." Juji T., Akesaka T., Kashiwase K. Submitted (JUL-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE OF 26-206 (ALLELE B*56:05).
[5]	"Identification of two new HLA-B22 variants, HLA-B*5509 and B*5606." Voorter C.E., Hepkema B.G., Mulkers E.M.T., Lems S.P.M., van den Berg-Loonen E.M. Tissue Antigens 58:42-46(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 26-206 (ALLELE B*56:06).
[6]	"Unexpected Bw4 and Bw6 reactivity patterns in new alleles." Voorter C.E., van der Vlies S., Kik M., van den Berg-Loonen E.M. Tissue Antigens 56:363-370(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 26-206 (ALLELE B*56:07).
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M77775 mRNA. Translation: AAA03690.1. M77776 mRNA. Translation: AAA03689.1. D85762 mRNA. Translation: BAA12869.1. AH005456 Genomic DNA. Translation: AAB62223.1. AB030574 Genomic DNA. Translation: BAA82677.1. Y18542, Y18543 Genomic DNA. Translation: CAC12884.1. Y18544, Y18545 Genomic DNA. Translation: CAC12896.1.
IPI	IPI00472284.
PIR	I72754. I72755.
UniGene	Hs.654404. Hs.77961.
3D structure databases
ProteinModelPortal	P30495.
ModBase	Search...
Protein-protein interaction databases
IntAct	P30495. 1 interaction.
Polymorphism databases
DMDM	231418.
Proteomic databases
PRIDE	P30495.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000359635; ENSP00000352656; ENSG00000206450.
Organism-specific databases
GeneCards	GC06M031321.
HGNC	HGNC:4932. HLA-B.
MIM	142830. gene.
neXtProt	NX_P30495.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	HBG016709.
InParanoid	P30495.
Enzyme and pathway databases
Reactome	REACT_6900. Immune System.
Gene expression databases
ArrayExpress	P30495.
CleanEx	HS_HLA-B.
Genevestigator	P30495.
Family and domain databases
Gene3D	2.60.40.10. 1 hit. 3.30.500.10. 1 hit.
InterPro	IPR007110. Ig-like_dom. IPR013783. Ig-like_fold. IPR003006. Ig/MHC_CS. IPR003597. Ig_C1-set. IPR011161. MHC_I-like_Ag-recog. IPR011162. MHC_I/II-like_Ag-recog. IPR001039. MHC_I_a_a1/a2. IPR010579. MHC_I_a_C. [Graphical view]
Pfam	PF07654. C1-set. 1 hit. PF00129. MHC_I. 1 hit. PF06623. MHC_I_C. 1 hit. [Graphical view]
PRINTS	PR01638. MHCCLASSI.
SMART	SM00407. IGc1. 1 hit. [Graphical view]
SUPFAM	SSF54452. MHC_I/II-like_Ag-recog. 1 hit.
PROSITE	PS50835. IG_LIKE. 1 hit. PS00290. IG_MHC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	HLA-B. human.
SOURCE	Search...

Entry information

Entry name

1B56_HUMAN

Accession

Primary (citable) accession number: P30495
Secondary accession number(s): O19758 , P30496, P79490, Q9GIM3, Q9GJ17, Q9TPR4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	May 1, 2013
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.