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Protein

HLA class I histocompatibility antigen, B-52 alpha chain

Gene

HLA-B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the presentation of foreign antigens to the immune system.

GO - Molecular functioni

  1. peptide antigen binding Source: UniProtKB

GO - Biological processi

  1. antigen processing and presentation of exogenous peptide antigen via MHC class I Source: Reactome
  2. antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent Source: Reactome
  3. antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent Source: Reactome
  4. antigen processing and presentation of peptide antigen via MHC class I Source: Reactome
  5. cytokine-mediated signaling pathway Source: Reactome
  6. interferon-gamma-mediated signaling pathway Source: Reactome
  7. positive regulation of T cell mediated cytotoxicity Source: InterPro
  8. regulation of immune response Source: Reactome
  9. type I interferon signaling pathway Source: Reactome
  10. viral process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Host-virus interaction, Immunity

Enzyme and pathway databases

ReactomeiREACT_111168. Endosomal/Vacuolar pathway.
REACT_111178. ER-Phagosome pathway.
REACT_11152. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
REACT_25078. Interferon gamma signaling.
REACT_25162. Interferon alpha/beta signaling.
REACT_75795. Antigen Presentation: Folding, assembly and peptide loading of class I MHC.

Names & Taxonomyi

Protein namesi
Recommended name:
HLA class I histocompatibility antigen, B-52 alpha chain
Alternative name(s):
Bw-52
HLA class I histocompatibility antigen, B-5 alpha chain
MHC class I antigen B*52
Gene namesi
Name:HLA-B
Synonyms:HLAB
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Unplaced

Organism-specific databases

HGNCiHGNC:4932. HLA-B.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini25 – 308284ExtracellularSequence AnalysisAdd
BLAST
Transmembranei309 – 33224HelicalSequence AnalysisAdd
BLAST
Topological domaini333 – 36230CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cell surface Source: UniProtKB
  2. early endosome membrane Source: Reactome
  3. endoplasmic reticulum Source: UniProtKB
  4. ER to Golgi transport vesicle membrane Source: Reactome
  5. Golgi apparatus Source: UniProtKB
  6. Golgi membrane Source: Reactome
  7. integral component of lumenal side of endoplasmic reticulum membrane Source: Reactome
  8. MHC class I protein complex Source: UniProtKB
  9. phagocytic vesicle membrane Source: Reactome
  10. plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Membrane, MHC I

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Add
BLAST
Chaini25 – 362338HLA class I histocompatibility antigen, B-52 alpha chainPRO_0000018855Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi110 – 1101N-linked (GlcNAc...)By similarity
Disulfide bondi125 ↔ 188PROSITE-ProRule annotation
Disulfide bondi227 ↔ 283PROSITE-ProRule annotation

Post-translational modificationi

Polyubiquitinated in a post ER compartment by interaction with human herpesvirus 8 MIR1 protein. This targets the protein for rapid degradation via the ubiquitin system (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Ubl conjugation

Proteomic databases

PRIDEiP30490.

Expressioni

Gene expression databases

CleanExiHS_HLA-B.
GenevestigatoriP30490.

Interactioni

Subunit structurei

Heterodimer of an alpha chain and a beta chain (beta-2-microglobulin). Interacts with human herpesvirus 8 MIR1 protein (By similarity).By similarity

Structurei

Secondary structure

1
362
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi27 – 3610Combined sources
Beta strandi39 – 413Combined sources
Beta strandi45 – 528Combined sources
Beta strandi55 – 617Combined sources
Beta strandi64 – 663Combined sources
Turni75 – 784Combined sources
Helixi81 – 10929Combined sources
Beta strandi118 – 12710Combined sources
Beta strandi133 – 14210Combined sources
Beta strandi145 – 1506Combined sources
Helixi162 – 17211Combined sources
Turni173 – 1753Combined sources
Helixi176 – 18510Combined sources
Helixi187 – 19812Combined sources
Helixi200 – 2034Combined sources
Beta strandi212 – 2176Combined sources
Beta strandi219 – 2213Combined sources
Beta strandi223 – 23513Combined sources
Beta strandi238 – 24710Combined sources
Helixi249 – 2513Combined sources
Beta strandi252 – 2543Combined sources
Beta strandi261 – 2633Combined sources
Beta strandi265 – 2739Combined sources
Helixi277 – 2804Combined sources
Beta strandi281 – 2866Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3W39X-ray3.10A/D25-300[»]
ProteinModelPortaliP30490.
SMRiP30490. Positions 25-300.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini209 – 29587Ig-like C1-typeAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni25 – 11490Alpha-1Add
BLAST
Regioni115 – 20692Alpha-2Add
BLAST
Regioni207 – 29892Alpha-3Add
BLAST
Regioni299 – 30810Connecting peptide

Sequence similaritiesi

Belongs to the MHC class I family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG016709.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.30.500.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
IPR011161. MHC_I-like_Ag-recog.
IPR011162. MHC_I/II-like_Ag-recog.
IPR027648. MHC_I_a.
IPR001039. MHC_I_a_a1/a2.
IPR010579. MHC_I_a_C.
[Graphical view]
PfamiPF07654. C1-set. 1 hit.
PF00129. MHC_I. 1 hit.
PF06623. MHC_I_C. 1 hit.
[Graphical view]
PRINTSiPR01638. MHCCLASSI.
SMARTiSM00407. IGc1. 1 hit.
[Graphical view]
SUPFAMiSSF54452. SSF54452. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30490-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVTAPRTVL LLLWGAVALT ETWAGSHSMR YFYTAMSRPG RGEPRFIAVG
60 70 80 90 100
YVDDTQFVRF DSDAASPRTE PRAPWIEQEG PEYWDRETQI SKTNTQTYRE
110 120 130 140 150
NLRIALRYYN QSEAGSHTWQ TMYGCDVGPD GRLLRGHNQY AYDGKDYIAL
160 170 180 190 200
NEDLSSWTAA DTAAQITQRK WEAAREAEQL RAYLEGLCVE WLRRHLENGK
210 220 230 240 250
ETLQRADPPK THVTHHPVSD HEATLRCWAL GFYPAEITLT WQRDGEDQTQ
260 270 280 290 300
DTELVETRPA GDRTFQKWAA VVVPSGEEQR YTCHVQHEGL PKPLTLRWEP
310 320 330 340 350
SSQSTIPIVG IVAGLAVLAV VVIGAVVATV MCRRKSSGGK GGSYSQAASS
360
DSAQGSDVSL TA
Length:362
Mass (Da):40,521
Last modified:April 1, 1993 - v1
Checksum:iA32E36370FD84F91
GO

Polymorphismi

The following alleles of B-52 are known: B*52:01, B*52:02 (B*52012V) and B*52:03. The sequence shown is that of B*52:01.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti69 – 702TE → MA in allele B*52:02.
VAR_016521
Natural varianti176 – 1761E → V in allele B*52:03.
VAR_016522
Natural varianti195 – 1951H → Y in allele B*52:03.
VAR_016523

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22799
, M22793, M22794, M22795, M22796, M22797, M22798 Genomic DNA. Translation: AAA59645.1. Sequence problems.
AH008246 Genomic DNA. Translation: AAF00933.1.
AH009592 Genomic DNA. Translation: AAF81610.1.
PIRiB30345.
UniGeneiHs.654404.
Hs.77961.

Genome annotation databases

EnsembliENST00000450871; ENSP00000388208; ENSG00000232126.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22799
, M22793, M22794, M22795, M22796, M22797, M22798 Genomic DNA. Translation: AAA59645.1. Sequence problems.
AH008246 Genomic DNA. Translation: AAF00933.1.
AH009592 Genomic DNA. Translation: AAF81610.1.
PIRiB30345.
UniGeneiHs.654404.
Hs.77961.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3W39X-ray3.10A/D25-300[»]
ProteinModelPortaliP30490.
SMRiP30490. Positions 25-300.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP30490.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000450871; ENSP00000388208; ENSG00000232126.

Organism-specific databases

GeneCardsiGC06M031321.
GC06Mm31398.
HGNCiHGNC:4932. HLA-B.
MIMi142830. gene.
neXtProtiNX_P30490.
GenAtlasiSearch...

Phylogenomic databases

HOVERGENiHBG016709.

Enzyme and pathway databases

ReactomeiREACT_111168. Endosomal/Vacuolar pathway.
REACT_111178. ER-Phagosome pathway.
REACT_11152. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
REACT_25078. Interferon gamma signaling.
REACT_25162. Interferon alpha/beta signaling.
REACT_75795. Antigen Presentation: Folding, assembly and peptide loading of class I MHC.

Miscellaneous databases

ChiTaRSiHLA-B. human.
SOURCEiSearch...

Gene expression databases

CleanExiHS_HLA-B.
GenevestigatoriP30490.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.30.500.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
IPR011161. MHC_I-like_Ag-recog.
IPR011162. MHC_I/II-like_Ag-recog.
IPR027648. MHC_I_a.
IPR001039. MHC_I_a_a1/a2.
IPR010579. MHC_I_a_C.
[Graphical view]
PfamiPF07654. C1-set. 1 hit.
PF00129. MHC_I. 1 hit.
PF06623. MHC_I_C. 1 hit.
[Graphical view]
PRINTSiPR01638. MHCCLASSI.
SMARTiSM00407. IGc1. 1 hit.
[Graphical view]
SUPFAMiSSF54452. SSF54452. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "HLA-B51 and HLA-Bw52 differ by only two amino acids which are in the helical region of the alpha 1 domain."
    Hayashi H., Ennis P.D., Ariga H., Salter R.D., Parham P., Kano K., Takiguchi M.
    J. Immunol. 142:306-311(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ALLELE B*52:01).
  2. "Novel HLA-B allele."
    Jones P.F., Hurley C.K.
    Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 26-206 (ALLELE B*52:02).
  3. "Novel HLA-B allele."
    Gans C.P., Hurley C.K.
    Submitted (JUN-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 26-206 (ALLELE B*52:03).
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  5. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry namei1B52_HUMAN
AccessioniPrimary (citable) accession number: P30490
Secondary accession number(s): Q9MY75, Q9TPT4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: March 4, 2015
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.