P30487 (1B49_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
HLA class I histocompatibility antigen, B-49 alpha chain

Alternative name(s):
HLA class I histocompatibility antigen, B-21 alpha chain
MHC class I antigen B*49

Gene names

Name:	HLA-B
Synonyms:	HLAB

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the presentation of foreign antigens to the immune system.
Subunit structure	Heterodimer of an alpha chain and a beta chain (beta-2-microglobulin). Interacts with human herpesvirus 8 MIR1 protein By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein.
Post-translational modification	Polyubiquitinated in a post ER compartment by interaction with human herpesvirus 8 MIR1 protein. This targets the protein for rapid degradation via the ubiquitin system By similarity.
Polymorphism	The following alleles of B-49 are known: B*49:01, B*49:02 and B*49:03 (B*RA). The sequence shown is that of B*49:01.
Sequence similarities	Belongs to the MHC class I family. Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

Ontologies

Keywords
Biological process	Host-virus interaction Immunity
Cellular component	MHC I Membrane
Coding sequence diversity	Polymorphism
Domain	Signal Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent Traceable author statement. Source: Reactome antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent Traceable author statement. Source: Reactome interferon-gamma-mediated signaling pathway Traceable author statement. Source: Reactome regulation of immune response Traceable author statement. Source: Reactome type I interferon-mediated signaling pathway Traceable author statement. Source: Reactome virus-host interaction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	ER to Golgi transport vesicle membrane Traceable author statement. Source: Reactome Golgi membrane Traceable author statement. Source: Reactome MHC class I protein complex Inferred from electronic annotation. Source: UniProtKB-KW early endosome membrane Traceable author statement. Source: Reactome integral to lumenal side of endoplasmic reticulum membrane Traceable author statement. Source: Reactome phagocytic vesicle membrane Traceable author statement. Source: Reactome plasma membrane Traceable author statement. Source: Reactome
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24
	Chain	25 – 362	338	HLA class I histocompatibility antigen, B-49 alpha chain		PRO_0000018852
Regions
	Topological domain	25 – 309	285	Extracellular Potential
	Transmembrane	310 – 333	24	Helical; Potential
	Topological domain	334 – 362	29	Cytoplasmic Potential
	Domain	209 – 295	87	Ig-like C1-type
	Region	25 – 114	90	Alpha-1
	Region	115 – 206	92	Alpha-2
	Region	207 – 298	92	Alpha-3
	Region	299 – 309	11	Connecting peptide
Amino acid modifications
	Glycosylation	110	1	N-linked (GlcNAc...) By similarity
	Disulfide bond	125 ↔ 188		By similarity
	Disulfide bond	227 ↔ 283		By similarity
Natural variations
	Natural variant	9	1	V → L. Corresponds to variant rs1050462 [ dbSNP | Ensembl ].		VAR_056403
	Natural variant	17	1	L → V. Corresponds to variant rs1131165 [ dbSNP | Ensembl ].		VAR_056404
	Natural variant	48	1	T → A in allele B*49:03.		VAR_016512
	Natural variant	56	1	L → Q in allele B*49:03.		VAR_016513
	Natural variant	65	1	T → A in allele B*49:03.		VAR_016514
	Natural variant	69	1	K → T in allele B*49:03.		VAR_016515
	Natural variant	104	1	I → T in allele B*49:02.		VAR_016516

Sequences

	Sequence		Length	Mass (Da)
	P30487 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: 77B1C82AD3DAEB4A	FASTA	362	40,581
	10 20 30 40 50 60 MRVTAPRTVL LLLSAALALT ETWAGSHSMR YFHTAMSRPG RGEPRFITVG YVDDTLFVRF 70 80 90 100 110 120 DSDATSPRKE PRAPWIEQEG PEYWDRETQI SKTNTQTYRE NLRIALRYYN QSEAGSHTWQ 130 140 150 160 170 180 RMYGCDLGPD GRLLRGYNQL AYDGKDYIAL NEDLSSWTAA DTAAQITQRK WEAAREAEQL 190 200 210 220 230 240 RAYLEGLCVE WLRRYLENGK ETLQRADPPK THVTHHPISD HEATLRCWAL GFYPAEITLT 250 260 270 280 290 300 WQRDGEDQTQ DTELVETRPA GDRTFQKWAA VVVPSGEEQR YTCHVQHEGL PKPLTLRWEP 310 320 330 340 350 360 SSQSTIPIVG IVAGLAVLAV VVIGAVVATV MCRRKSSGGK GGSYSQAASS DSAQGSDVSL TA

References

[1]	"Diversity and diversification of HLA-A,B,C alleles." Parham P., Lawlor D.A., Lomen C.E., Ennis P.D. J. Immunol. 142:3937-3950(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (ALLELE B*49:01).
[2]	"Serologic cross-reactivities poorly reflect allelic relationships in the HLA-B12 and HLA-B21 groups. Dominant epitopes of the alpha 2 helix." Hildebrand W.H., Madrigal J.A., Belich M.P., Zemmour J., Ward F.E., Williams R.C., Parham P. J. Immunol. 149:3563-3568(1992) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION TO 78.
[3]	Hurley C.K., Bei M., Rodriguez S., Johnson A. Submitted (JUN-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE OF 1-322 (ALLELE B*49:01).
[4]	"Novel HLA-B Allele." Gans C.P., Hurley C.K. Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE OF 26-206 (ALLELE B*49:02).
[5]	"Sequence, genetics and serology of a new HLA-B allele: B*4903." Darke C., Street J., Guttridge M.G. Tissue Antigens 57:478-480(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 26-206 (ALLELE B*49:03).
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M24037 mRNA. Translation: AAA02950.1. U11263 mRNA. Translation: AAA19923.1. AH009483 Genomic DNA. Translation: AAF78054.1. AJ288980 Genomic DNA. Translation: CAB88084.1.
IPI	IPI00472456.
UniGene	Hs.654404. Hs.77961.
3D structure databases
ProteinModelPortal	P30487.
ModBase	Search...
Polymorphism databases
DMDM	1345599.
Proteomic databases
PRIDE	P30487.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000435618; ENSP00000405178; ENSG00000224608.
Organism-specific databases
GeneCards	GC06M031321.
H-InvDB	HIX0166654.
HGNC	HGNC:4932. HLA-B.
MIM	142830. gene.
neXtProt	NX_P30487.
GenAtlas	Search...
Phylogenomic databases
HOVERGEN	HBG016709.
InParanoid	P30487.
Enzyme and pathway databases
Reactome	REACT_6900. Immune System.
Gene expression databases
ArrayExpress	P30487.
CleanEx	HS_HLA-B.
Genevestigator	P30487.
Family and domain databases
Gene3D	2.60.40.10. 1 hit. 3.30.500.10. 1 hit.
InterPro	IPR007110. Ig-like_dom. IPR013783. Ig-like_fold. IPR003006. Ig/MHC_CS. IPR003597. Ig_C1-set. IPR011161. MHC_I-like_Ag-recog. IPR011162. MHC_I/II-like_Ag-recog. IPR001039. MHC_I_a_a1/a2. IPR010579. MHC_I_a_C. [Graphical view]
Pfam	PF07654. C1-set. 1 hit. PF00129. MHC_I. 1 hit. PF06623. MHC_I_C. 1 hit. [Graphical view]
PRINTS	PR01638. MHCCLASSI.
SMART	SM00407. IGc1. 1 hit. [Graphical view]
SUPFAM	SSF54452. MHC_I/II-like_Ag-recog. 1 hit.
PROSITE	PS50835. IG_LIKE. 1 hit. PS00290. IG_MHC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	HLA-B. human.
SOURCE	Search...

Entry information

Entry name

1B49_HUMAN

Accession

Primary (citable) accession number: P30487
Secondary accession number(s): O19625, Q9MY37, Q9MY93

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 107 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.