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P30464 (1B15_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 126. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
HLA class I histocompatibility antigen, B-15 alpha chain
Alternative name(s):
MHC class I antigen B*15
Gene names
Name:HLA-B
Synonyms:HLAB
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length362 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the presentation of foreign antigens to the immune system.

Subunit structure

Heterodimer of an alpha chain and a beta chain (beta-2-microglobulin). Interacts with human herpesvirus 8 MIR1 protein By similarity.

Subcellular location

Membrane; Single-pass type I membrane protein.

Post-translational modification

Polyubiquitinated in a post ER compartment by interaction with human herpesvirus 8 MIR1 protein. This targets the protein for rapid degradation via the ubiquitin system By similarity.

Polymorphism

The following alleles of B-15 are known: B*15:01 (Bw-62; B-62), B*15:02 (Bw-75, B-75), B*15:03 (Bw-72; B-72; B-70) B*15:04, B*15:11, B*15:19 and B*15:66. The sequence shown is that of B*15:01.

Involvement in disease

Stevens-Johnson syndrome (SJS) [MIM:608579]: A rare blistering mucocutaneous disease that share clinical and histopathologic features with toxic epidermal necrolysis. Both disorders are characterized by high fever, malaise, and a rapidly developing blistering exanthema of macules and target-like lesions accompanied by mucosal involvement. Stevens-Johnson syndrome is a milder disease characterized by destruction and detachment of the skin epithelium and mucous membranes involving less than 10% of the body surface area. Ocular symptoms include ulcerative conjunctivitis, keratitis, iritis, uveitis and sometimes blindness. It can be caused by a severe adverse reaction to particular types of medication, although Mycoplasma infections may induce some cases.
Note: Disease susceptibility is associated with variations affecting the gene represented in this entry. Increased susceptibility to Stevens-Johnson syndrome is conferred by allele B*15:02.

Sequence similarities

Belongs to the MHC class I family.

Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

Ontologies

Keywords
   Biological processHost-virus interaction
Immunity
   Cellular componentMembrane
MHC I
   Coding sequence diversityPolymorphism
   DomainSignal
Transmembrane
Transmembrane helix
   PTMDisulfide bond
Glycoprotein
Ubl conjugation
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processantigen processing and presentation of exogenous peptide antigen via MHC class I

Traceable author statement. Source: Reactome

antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent

Traceable author statement. Source: Reactome

antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent

Traceable author statement. Source: Reactome

antigen processing and presentation of peptide antigen via MHC class I

Traceable author statement. Source: Reactome

cytokine-mediated signaling pathway

Traceable author statement. Source: Reactome

interferon-gamma-mediated signaling pathway

Traceable author statement. Source: Reactome

positive regulation of T cell mediated cytotoxicity

Inferred from electronic annotation. Source: InterPro

regulation of immune response

Traceable author statement. Source: Reactome

type I interferon signaling pathway

Traceable author statement. Source: Reactome

viral process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentER to Golgi transport vesicle membrane

Traceable author statement. Source: Reactome

Golgi membrane

Traceable author statement. Source: Reactome

MHC class I protein complex

Inferred from sequence or structural similarity. Source: UniProt

cell surface

Inferred from sequence or structural similarity. Source: UniProt

early endosome membrane

Traceable author statement. Source: Reactome

integral component of lumenal side of endoplasmic reticulum membrane

Traceable author statement. Source: Reactome

phagocytic vesicle membrane

Traceable author statement. Source: Reactome

plasma membrane

Traceable author statement. Source: Reactome

   Molecular_functionpeptide antigen binding

Inferred from sequence or structural similarity. Source: UniProt

receptor binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424
Chain25 – 362338HLA class I histocompatibility antigen, B-15 alpha chain
PRO_0000018837

Regions

Topological domain25 – 309285Extracellular Potential
Transmembrane310 – 33324Helical; Potential
Topological domain334 – 36229Cytoplasmic Potential
Domain209 – 29587Ig-like C1-type
Region25 – 11490Alpha-1
Region115 – 20692Alpha-2
Region207 – 29892Alpha-3
Region299 – 30911Connecting peptide

Amino acid modifications

Glycosylation1101N-linked (GlcNAc...) By similarity
Disulfide bond125 ↔ 188 Ref.14
Disulfide bond227 ↔ 283 Ref.14

Natural variations

Natural variant481A → S in allele B*15:03.
VAR_016365
Natural variant69 – 702MA → EE in allele B*15:03.
VAR_016366
Natural variant871E → N in allele B*15:02 and allele B*15:11; requires 2 nucleotide substitutions.
VAR_016367
Natural variant911S → C in allele B*15:66.
VAR_016368
Natural variant911S → Y in allele B*15:11.
VAR_016369
Natural variant118 – 1192TL → II in allele B*15:02.
VAR_016370
Natural variant1191L → W in allele B*15:04.
VAR_016371
Natural variant1211R → T in allele B*15:04.
VAR_016372
Natural variant1371H → Y in allele B*15:02.
VAR_016373
Natural variant1801W → L in allele B*15:02 and allele B*15:03.
VAR_016374
Natural variant190 – 1912EW → DG in allele B*15:19.
VAR_016375
Natural variant2741P → L in allele B*15:19.
VAR_016376

Secondary structure

................................................. 362
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P30464 [UniParc].

Last modified August 22, 2003. Version 2.
Checksum: 86E938087372EBE0

FASTA36240,388
        10         20         30         40         50         60 
MRVTAPRTVL LLLSGALALT ETWAGSHSMR YFYTAMSRPG RGEPRFIAVG YVDDTQFVRF 

        70         80         90        100        110        120 
DSDAASPRMA PRAPWIEQEG PEYWDRETQI SKTNTQTYRE SLRNLRGYYN QSEAGSHTLQ 

       130        140        150        160        170        180 
RMYGCDVGPD GRLLRGHDQS AYDGKDYIAL NEDLSSWTAA DTAAQITQRK WEAAREAEQW 

       190        200        210        220        230        240 
RAYLEGLCVE WLRRYLENGK ETLQRADPPK THVTHHPISD HEATLRCWAL GFYPAEITLT 

       250        260        270        280        290        300 
WQRDGEDQTQ DTELVETRPA GDRTFQKWAA VVVPSGEEQR YTCHVQHEGL PKPLTLRWEP 

       310        320        330        340        350        360 
SSQSTIPIVG IVAGLAVLAV VVIGAVVATV MCRRKSSGGK GGSYSQAASS DSAQGSDVSL 


TA 

« Hide

References

« Hide 'large scale' references
[1]"The HLA-Bw75 subtype of B15: molecular characterization and comparison with crossreacting antigens."
Little A.-M., Parham P.
Tissue Antigens 38:186-190(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ALLELE B*15:02).
[2]"HLA-B15: a widespread and diverse family of HLA-B alleles."
Hildebrand W.H., Domena J.D., Shen S.Y., Lau M., Terasaki P.I., Bunce M., Marsh S.G.E., Guttridge M.G., Bias W.B., Parham P.
Tissue Antigens 43:209-218(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ALLELES B*15:01; B*15:11 AND B*15:19).
[3]"Further molecular diversity in the HLA-B15 group."
Lin L., Tokunaga K., Tanaka H., Nakajima F., Imanishi T., Kashiwase K., Bannai M., Mizuno S., Akaza T., Tadokoro K., Shibata Y., Juji T.
Tissue Antigens 47:265-274(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ALLELES B*15:01; B*15:02 AND B*15:11).
Tissue: Blood.
[4]"Distinctive HLA-A,B antigens of black populations formed by interallelic conversion."
Madrigal J.A., Belich M.P., Hildebrand W.H., Benjamin R.J., Little A.-M., Zemmour J., Ennis P.D., Ward F.E., Petzl-Erler M.L., Martell R.W., du Toit E.D., Parham P.
J. Immunol. 149:3411-3415(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE (ALLELE B*15:03).
[5]"New recombinant HLA-B alleles in a tribe of South American Amerindians indicate rapid evolution of MHC class I loci."
Watkins D.I., McAdam S.N., Liu X., Stang C.R., Milford E.L., Levine C.G., Garber T.L., Dogon A.L., Lord C.I., Ghim S.H., Troup G.M., Hughes A.L., Letvin N.L.
Nature 357:329-333(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE (ALLELE B*15:04).
[6]Ramos M., Barber D.F., Layrisse Z., de Castro J.A.
Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE (ALLELE B*15:04).
Tissue: Blood.
[7]"Cloning and sequencing full-length HLA-B and -C genes."
Cox S.T., McWhinnie A.J., Robinson J., Marsh S.G.E., Parham P., Madrigal J.A., Little A.-M.
Tissue Antigens 61:20-48(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE (ALLELE B*15:01).
Tissue: Blood.
[8]"Identification of HLA-B*1566."
Cox S.T., Prokupek B., Baker F., Holman R., Leung V.T., Wong A.S., Madrigal J.A., Little A.-M.
Tissue Antigens 59:424-425(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE (ALLELE B*15:66).
[9]"The DNA sequence and analysis of human chromosome 6."
Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[10]"Allelic variation in HLA-B and HLA-C sequences and the evolution of the HLA-B alleles."
Pohla H., Kuon W., Tabaczewski P., Doerner C., Weiss E.H.
Immunogenetics 29:297-307(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-362 (ALLELE B*15:01).
[11]"Allelic variations clustered in the antigen binding sites of HLA-Bw62 molecules."
Choo S.Y., Fan L.A., Hansen J.A.
Immunogenetics 37:108-113(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 25-362 (ALLELE B*15:01).
[12]"Medical genetics: a marker for Stevens-Johnson syndrome."
Chung W.-H., Hung S.-I., Hong H.-S., Hsih M.-S., Yang L.-C., Ho H.-C., Wu J.-Y., Chen Y.-T.
Nature 428:486-486(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: ASSOCIATION OF ALLELE B*15:02 WITH STEVENS-JOHNSON SYNDROME.
[13]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[14]"Crystal structures of two peptide-HLA-B*1501 complexes; structural characterization of the HLA-B62 supertype."
Roder G., Blicher T., Justesen S., Johannesen B., Kristensen O., Kastrup J., Buus S., Gajhede M.
Acta Crystallogr. D 62:1300-1310(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.79 ANGSTROMS) OF 25-300 (ALLELE B*15:01) IN COMPLEX WITH EBV NUCLEAR ANTIGEN AND UBE2 PEPTIDES, DISULFIDE BONDS.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M75138 mRNA. Translation: AAA59630.1.
U03859 mRNA. Translation: AAA03601.1.
U03027 mRNA. Translation: AAA18902.1.
L11604 mRNA. Translation: AAA56836.1.
D50292 mRNA. Translation: BAA08823.1.
D50293 mRNA. Translation: BAA08824.1.
D50294 mRNA. Translation: BAA08825.1.
X61709 mRNA. Translation: CAA43878.1.
M84382 mRNA. Translation: AAA59632.1.
U70528 mRNA. Translation: AAB16918.1.
AJ295140 Genomic DNA. Translation: CAC17462.1.
AJ308399 Genomic DNA. Translation: CAC33441.1.
CR759828 Genomic DNA. Translation: CAQ10738.1.
M28203 mRNA. Translation: AAA53258.1.
M83193 mRNA. Translation: AAA58628.1.
PIRG01230.
I38421.
I62042.
S16789.
S24433.
S77966.
UniGeneHs.654404.
Hs.77961.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1XR8X-ray2.30A25-300[»]
1XR9X-ray1.79A25-300[»]
3C9NX-ray1.87A25-300[»]
ProteinModelPortalP30464.
SMRP30464. Positions 25-300.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP30464. 1 interaction.

Polymorphism databases

DMDM34305703.

Proteomic databases

PRIDEP30464.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000450871; ENSP00000388208; ENSG00000232126.
UCSCuc011hpo.2. human.

Organism-specific databases

GeneCardsGC06M031321.
GC06Mm31398.
HGNCHGNC:4932. HLA-B.
MIM142830. gene.
608579. phenotype.
neXtProtNX_P30464.
Orphanet240841. Abacavir toxicity.
240845. Allopurinol toxicity.
825. Ankylosing spondylitis.
117. Behcet disease.
240871. Flucloxacilline toxicity.
240901. Fosphenytoin toxicity.
240899. Phenytoin toxicity.
275798. Pulmonary arterial hypertension associated with connective tissue disease.
36426. Stevens-Johnson syndrome.
241001. Susceptibility to hepatitis due to flucloxacilline treatment.
241005. Susceptibility to hypersensitivity syndrome due to abacavir treatment.
241035. Susceptibility to toxic epidermal necrolysis due to allopurinol treatment.
241037. Susceptibility to toxic epidermal necrolysis due to carbamazepine treatment.
241039. Susceptibility to toxic epidermal necrolysis due to phenytoin treatment.
241041. Susceptibility to toxic epidermal necrolysis due to phosphenytoin treatment.
3287. Takayasu arteritis.
GenAtlasSearch...

Phylogenomic databases

HOVERGENHBG016709.
InParanoidP30464.
OMAWERETRV.
PhylomeDBP30464.

Enzyme and pathway databases

ReactomeREACT_6900. Immune System.

Gene expression databases

CleanExHS_HLA-B.
GenevestigatorP30464.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
3.30.500.10. 1 hit.
InterProIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
IPR011161. MHC_I-like_Ag-recog.
IPR011162. MHC_I/II-like_Ag-recog.
IPR027648. MHC_I_a.
IPR001039. MHC_I_a_a1/a2.
IPR010579. MHC_I_a_C.
[Graphical view]
PfamPF07654. C1-set. 1 hit.
PF00129. MHC_I. 1 hit.
PF06623. MHC_I_C. 1 hit.
[Graphical view]
PRINTSPR01638. MHCCLASSI.
SMARTSM00407. IGc1. 1 hit.
[Graphical view]
SUPFAMSSF54452. SSF54452. 1 hit.
PROSITEPS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSHLA-B. human.
EvolutionaryTraceP30464.
SOURCESearch...

Entry information

Entry name1B15_HUMAN
AccessionPrimary (citable) accession number: P30464
Secondary accession number(s): B0V0B8 expand/collapse secondary AC list , P30465, P30513, Q29633, Q29636, Q29829, Q29953, Q29982, Q95343, Q95344, Q9BD06
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: August 22, 2003
Last modified: April 16, 2014
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 6

Human chromosome 6: entries, gene names and cross-references to MIM