P30464 (1B15_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
HLA class I histocompatibility antigen, B-15 alpha chain

Alternative name(s):
MHC class I antigen B*15

Gene names

Name:	HLA-B
Synonyms:	HLAB

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the presentation of foreign antigens to the immune system.
Subunit structure	Heterodimer of an alpha chain and a beta chain (beta-2-microglobulin). Interacts with human herpesvirus 8 MIR1 protein By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein.
Post-translational modification	Polyubiquitinated in a post ER compartment by interaction with human herpesvirus 8 MIR1 protein. This targets the protein for rapid degradation via the ubiquitin system By similarity.
Polymorphism	The following alleles of B-15 are known: B*15:01 (Bw-62; B-62), B*15:02 (Bw-75, B-75), B*15:03 (Bw-72; B-72; B-70) B*15:04, B*15:11, B*15:19 and B*15:66. The sequence shown is that of B*15:01.
Involvement in disease	Stevens-Johnson syndrome (SJS) [MIM:608579]: A rare blistering mucocutaneous disease that share clinical and histopathologic features with toxic epidermal necrolysis. Both disorders are characterized by high fever, malaise, and a rapidly developing blistering exanthema of macules and target-like lesions accompanied by mucosal involvement. Stevens-Johnson syndrome is a milder disease characterized by destruction and detachment of the skin epithelium and mucous membranes involving less than 10% of the body surface area. Ocular symptoms include ulcerative conjunctivitis, keratitis, iritis, uveitis and sometimes blindness. It can be caused by a severe adverse reaction to particular types of medication, although Mycoplasma infections may induce some cases. Note: Disease susceptibility is associated with variations affecting the gene represented in this entry. Increased susceptibility to Stevens-Johnson syndrome is conferred by allele B*15:02.
Sequence similarities	Belongs to the MHC class I family. Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

Ontologies

Keywords
Biological process	Host-virus interaction Immunity
Cellular component	MHC I Membrane
Coding sequence diversity	Polymorphism
Domain	Signal Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein Ubl conjugation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent Traceable author statement. Source: Reactome antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent Traceable author statement. Source: Reactome interferon-gamma-mediated signaling pathway Traceable author statement. Source: Reactome regulation of immune response Traceable author statement. Source: Reactome type I interferon-mediated signaling pathway Traceable author statement. Source: Reactome virus-host interaction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	ER to Golgi transport vesicle membrane Traceable author statement. Source: Reactome Golgi membrane Traceable author statement. Source: Reactome MHC class I protein complex Inferred from electronic annotation. Source: UniProtKB-KW early endosome membrane Traceable author statement. Source: Reactome integral to lumenal side of endoplasmic reticulum membrane Traceable author statement. Source: Reactome phagocytic vesicle membrane Traceable author statement. Source: Reactome plasma membrane Traceable author statement. Source: Reactome
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 24

24

Chain

25 – 362

338

HLA class I histocompatibility antigen, B-15 alpha chain

PRO_0000018837

Regions

Topological domain

25 – 309

285

Extracellular Potential

Transmembrane

310 – 333

24

Helical; Potential

Topological domain

334 – 362

29

Cytoplasmic Potential

Domain

209 – 295

87

Ig-like C1-type

Region

25 – 114

90

Alpha-1

Region

115 – 206

92

Alpha-2

Region

207 – 298

92

Alpha-3

Region

299 – 309

11

Connecting peptide

Amino acid modifications

Glycosylation

110

1

N-linked (GlcNAc...) By similarity

Disulfide bond

125 ↔ 188

Ref.13

Disulfide bond

227 ↔ 283

Ref.13

Natural variations

Natural variant

48

1

A → S in allele B*15:03.

VAR_016365

Natural variant

69 – 70

2

MA → EE in allele B*15:03.

VAR_016366

Natural variant

87

1

E → N in allele B*15:02 and allele B*15:11; requires 2 nucleotide substitutions.

VAR_016367

Natural variant

91

1

S → C in allele B*15:66.

VAR_016368

Natural variant

91

1

S → Y in allele B*15:11.

VAR_016369

Natural variant

118 – 119

2

TL → II in allele B*15:02.

VAR_016370

Natural variant

119

1

L → W in allele B*15:04.

VAR_016371

Natural variant

121

1

R → T in allele B*15:04.

VAR_016372

Natural variant

137

1

H → Y in allele B*15:02.

VAR_016373

Natural variant

180

1

W → L in allele B*15:02 and allele B*15:03.

VAR_016374

Natural variant

190 – 191

2

EW → DG in allele B*15:19.

VAR_016375

Natural variant

274

1

P → L in allele B*15:19.

VAR_016376

Secondary structure

1

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362

Helix Strand Turn

Details...

Beta strand

27 – 36

10

Beta strand

41 – 43

3

Beta strand

45 – 52

8

Beta strand

55 – 61

7

Beta strand

64 – 66

3

Helix

74 – 76

3

Helix

81 – 108

28

Beta strand

118 – 127

10

Beta strand

133 – 142

10

Beta strand

145 – 150

6

Beta strand

157 – 161

5

Helix

162 – 173

12

Helix

176 – 185

10

Helix

187 – 198

12

Helix

200 – 203

4

Beta strand

210 – 217

8

Beta strand

219 – 235

17

Beta strand

238 – 243

6

Helix

249 – 251

3

Beta strand

261 – 263

3

Beta strand

265 – 274

10

Helix

278 – 280

3

Beta strand

281 – 286

6

Beta strand

290 – 292

3

Beta strand

294 – 296

3

Sequences

	Sequence		Length	Mass (Da)
	P30464 [UniParc]. Last modified August 22, 2003. Version 2. Checksum: 86E938087372EBE0	FASTA	362	40,388
	10 20 30 40 50 60 MRVTAPRTVL LLLSGALALT ETWAGSHSMR YFYTAMSRPG RGEPRFIAVG YVDDTQFVRF 70 80 90 100 110 120 DSDAASPRMA PRAPWIEQEG PEYWDRETQI SKTNTQTYRE SLRNLRGYYN QSEAGSHTLQ 130 140 150 160 170 180 RMYGCDVGPD GRLLRGHDQS AYDGKDYIAL NEDLSSWTAA DTAAQITQRK WEAAREAEQW 190 200 210 220 230 240 RAYLEGLCVE WLRRYLENGK ETLQRADPPK THVTHHPISD HEATLRCWAL GFYPAEITLT 250 260 270 280 290 300 WQRDGEDQTQ DTELVETRPA GDRTFQKWAA VVVPSGEEQR YTCHVQHEGL PKPLTLRWEP 310 320 330 340 350 360 SSQSTIPIVG IVAGLAVLAV VVIGAVVATV MCRRKSSGGK GGSYSQAASS DSAQGSDVSL TA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The HLA-Bw75 subtype of B15: molecular characterization and comparison with crossreacting antigens." Little A.-M., Parham P. Tissue Antigens 38:186-190(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ALLELE B*15:02).
[2]	"HLA-B15: a widespread and diverse family of HLA-B alleles." Hildebrand W.H., Domena J.D., Shen S.Y., Lau M., Terasaki P.I., Bunce M., Marsh S.G.E., Guttridge M.G., Bias W.B., Parham P. Tissue Antigens 43:209-218(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ALLELES B*15:01; B*15:11 AND B*15:19).
[3]	"Further molecular diversity in the HLA-B15 group." Lin L., Tokunaga K., Tanaka H., Nakajima F., Imanishi T., Kashiwase K., Bannai M., Mizuno S., Akaza T., Tadokoro K., Shibata Y., Juji T. Tissue Antigens 47:265-274(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ALLELES B*15:01; B*15:02 AND B*15:11). Tissue: Blood.
[4]	"Distinctive HLA-A,B antigens of black populations formed by interallelic conversion." Madrigal J.A., Belich M.P., Hildebrand W.H., Benjamin R.J., Little A.-M., Zemmour J., Ennis P.D., Ward F.E., Petzl-Erler M.L., Martell R.W., du Toit E.D., Parham P. J. Immunol. 149:3411-3415(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (ALLELE B*15:03).
[5]	"New recombinant HLA-B alleles in a tribe of South American Amerindians indicate rapid evolution of MHC class I loci." Watkins D.I., McAdam S.N., Liu X., Stang C.R., Milford E.L., Levine C.G., Garber T.L., Dogon A.L., Lord C.I., Ghim S.H., Troup G.M., Hughes A.L., Letvin N.L. Nature 357:329-333(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (ALLELE B*15:04).
[6]	Ramos M., Barber D.F., Layrisse Z., de Castro J.A. Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE (ALLELE B*15:04). Tissue: Blood.
[7]	"Cloning and sequencing full-length HLA-B and -C genes." Cox S.T., McWhinnie A.J., Robinson J., Marsh S.G.E., Parham P., Madrigal J.A., Little A.-M. Tissue Antigens 61:20-48(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (ALLELE B*15:01). Tissue: Blood.
[8]	"Identification of HLA-B*1566." Cox S.T., Prokupek B., Baker F., Holman R., Leung V.T., Wong A.S., Madrigal J.A., Little A.-M. Tissue Antigens 59:424-425(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (ALLELE B*15:66).
[9]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[10]	"Allelic variation in HLA-B and HLA-C sequences and the evolution of the HLA-B alleles." Pohla H., Kuon W., Tabaczewski P., Doerner C., Weiss E.H. Immunogenetics 29:297-307(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-362 (ALLELE B*15:01).
[11]	"Allelic variations clustered in the antigen binding sites of HLA-Bw62 molecules." Choo S.Y., Fan L.A., Hansen J.A. Immunogenetics 37:108-113(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 25-362 (ALLELE B*15:01).
[12]	"Medical genetics: a marker for Stevens-Johnson syndrome." Chung W.-H., Hung S.-I., Hong H.-S., Hsih M.-S., Yang L.-C., Ho H.-C., Wu J.-Y., Chen Y.-T. Nature 428:486-486(2004) [PubMed] [Europe PMC] [Abstract] Cited for: ASSOCIATION OF ALLELE B*15:02 WITH STEVENS-JOHNSON SYNDROME.
[13]	"Crystal structures of two peptide-HLA-B*1501 complexes; structural characterization of the HLA-B62 supertype." Roder G., Blicher T., Justesen S., Johannesen B., Kristensen O., Kastrup J., Buus S., Gajhede M. Acta Crystallogr. D 62:1300-1310(2006) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.79 ANGSTROMS) OF 25-300 (ALLELE B*15:01) IN COMPLEX WITH EBV NUCLEAR ANTIGEN AND UBE2 PEPTIDES, DISULFIDE BONDS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M75138 mRNA. Translation: AAA59630.1.
U03859 mRNA. Translation: AAA03601.1.
U03027 mRNA. Translation: AAA18902.1.
L11604 mRNA. Translation: AAA56836.1.
D50292 mRNA. Translation: BAA08823.1.
D50293 mRNA. Translation: BAA08824.1.
D50294 mRNA. Translation: BAA08825.1.
X61709 mRNA. Translation: CAA43878.1.
M84382 mRNA. Translation: AAA59632.1.
U70528 mRNA. Translation: AAB16918.1.
AJ295140 Genomic DNA. Translation: CAC17462.1.
AJ308399 Genomic DNA. Translation: CAC33441.1.
CR759828 Genomic DNA. Translation: CAQ10738.1.
M28203 mRNA. Translation: AAA53258.1.
M83193 mRNA. Translation: AAA58628.1.

IPI

IPI00646083.

PIR

G01230.
I38421.
I62042.
S16789.
S24433.
S77966.

UniGene

Hs.654404.
Hs.77961.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1XR8	X-ray	2.30	A	25-300	[»]
1XR9	X-ray	1.79	A	25-300	[»]
3C9N	X-ray	1.87	A	25-300	[»]

ProteinModelPortal

P30464.

ModBase

Search...

Protein-protein interaction databases

IntAct

P30464. 1 interaction.

Polymorphism databases

DMDM

34305703.

Proteomic databases

PRIDE

P30464.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000450871; ENSP00000388208; ENSG00000232126.

UCSC

uc011hpo.2. human.

Organism-specific databases

GeneCards

GC06M031321.

HGNC

HGNC:4932. HLA-B.

MIM

142830. gene.
608579. phenotype.

neXtProt

NX_P30464.

GenAtlas

Search...

Phylogenomic databases

HOVERGEN

HBG016709.

InParanoid

P30464.

OrthoDB

EOG4F4SBG.

Enzyme and pathway databases

Reactome

REACT_6900. Immune System.

Gene expression databases

ArrayExpress

P30464.

CleanEx

HS_HLA-B.

Genevestigator

P30464.

Family and domain databases

Gene3D

2.60.40.10. 1 hit.
3.30.500.10. 1 hit.

InterPro

IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
IPR011161. MHC_I-like_Ag-recog.
IPR011162. MHC_I/II-like_Ag-recog.
IPR001039. MHC_I_a_a1/a2.
IPR010579. MHC_I_a_C.
[Graphical view]

Pfam

PF07654. C1-set. 1 hit.
PF00129. MHC_I. 1 hit.
PF06623. MHC_I_C. 1 hit.
[Graphical view]

PRINTS

PR01638. MHCCLASSI.

SMART

SM00407. IGc1. 1 hit.
[Graphical view]

SUPFAM

SSF54452. MHC_I/II-like_Ag-recog. 1 hit.

PROSITE

PS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

HLA-B. human.

EvolutionaryTrace

P30464.

SOURCE

Search...

Entry information

Entry name

1B15_HUMAN

Accession

Primary (citable) accession number: P30464
Secondary accession number(s): B0V0B8 , P30465, P30513, Q29633, Q29636, Q29829, Q29953, Q29982, Q95343, Q95344, Q9BD06

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	August 22, 2003
Last modified:	May 1, 2013
This is version 118 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.