P30443 (1A01_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
HLA class I histocompatibility antigen, A-1 alpha chain

Alternative name(s):
MHC class I antigen A*1

Gene names

Name:	HLA-A
Synonyms:	HLAA

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	365 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the presentation of foreign antigens to the immune system.
Subunit structure	Dimer of alpha chain and a beta chain (beta-2-microglobulin). Interacts with human herpesvirus 8 MIR1 protein By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein.
Post-translational modification	Sulfated. Polyubiquitinated in a post ER compartment through interaction with human herpesvirus 8 MIR1 protein. This targets the protein for rapid degradation via the ubiquitin system By similarity.
Polymorphism	The following alleles of A-1 are known: A*01:01, A*01:02, A*01:03, A*01:06 and A*01:07. The sequence shown is that of A*01:01.
Sequence similarities	Belongs to the MHC class I family. Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

Ontologies

Keywords
Biological process	Host-virus interaction Immunity
Cellular component	MHC I Membrane
Coding sequence diversity	Polymorphism
Domain	Immunoglobulin domain Signal Transmembrane Transmembrane helix
PTM	Disulfide bond Glycoprotein Sulfation Ubl conjugation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	antigen processing and presentation of peptide antigen via MHC class I Traceable author statement. Source: Reactome interferon-gamma-mediated signaling pathway Traceable author statement. Source: Reactome interspecies interaction between organisms Inferred from electronic annotation. Source: UniProtKB-KW regulation of immune response Traceable author statement. Source: Reactome type I interferon-mediated signaling pathway Traceable author statement. Source: Reactome
Cellular component	ER to Golgi transport vesicle membrane Traceable author statement. Source: Reactome Golgi membrane Traceable author statement. Source: Reactome MHC class I protein complex Inferred from electronic annotation. Source: UniProtKB-KW integral to lumenal side of endoplasmic reticulum membrane Traceable author statement. Source: Reactome integral to plasma membrane Non-traceable author statement Ref.2. Source: UniProtKB
Molecular function	MHC class I receptor activity Non-traceable author statement Ref.2. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 24

24

Chain

25 – 365

341

HLA class I histocompatibility antigen, A-1 alpha chain

PRO_0000018813

Regions

Topological domain

25 – 308

284

Extracellular Potential

Transmembrane

309 – 332

24

Helical; Potential

Topological domain

333 – 365

33

Cytoplasmic Potential

Domain

209 – 295

87

Ig-like C1-type

Region

25 – 114

90

Alpha-1

Region

115 – 206

92

Alpha-2

Region

207 – 298

92

Alpha-3

Region

299 – 308

10

Connecting peptide

Amino acid modifications

Modified residue

83

1

Sulfotyrosine Potential

Glycosylation

110

1

N-linked (GlcNAc...) Ref.14

Disulfide bond

125 ↔ 188

By similarity

Disulfide bond

227 ↔ 283

By similarity

Natural variations

Natural variant

33

1

F → S in allele A*01:02.
Corresponds to variant rs2075684 [ dbSNP | Ensembl ].

VAR_004332

Natural variant

41

1

R → S in allele A*01:02.

VAR_004333

Natural variant

80

1

G → R in allele A*01:07.

VAR_016719

Natural variant

89

1

R → G.
Corresponds to variant rs1059459 [ dbSNP | Ensembl ].

VAR_056247

Natural variant

91

1

M → V in allele A*01:07.

VAR_016720

Natural variant

100

1

A → E in allele A*01:07.

VAR_016721

Natural variant

114

1

D → A in allele A*01:07.

VAR_016722

Natural variant

121

1

I → M in allele A*01:03.

VAR_016723

Natural variant

131

1

G → W.
Corresponds to variant rs1136702 [ dbSNP | Ensembl ].

VAR_056248

Natural variant

133

1

F → L.
Corresponds to variant rs1059488 [ dbSNP | Ensembl ].

VAR_056249

Natural variant

151

1

N → K.
Corresponds to variant rs1059509 [ dbSNP | Ensembl ].

VAR_056250

Natural variant

166

1

I → T.
Corresponds to variant rs1059516 [ dbSNP | Ensembl ].

VAR_056251

Natural variant

169

1

R → H.
Corresponds to variant rs1059520 [ dbSNP | Ensembl ].

VAR_056252

Natural variant

180

1

R → L in allele A*01:06.

VAR_016724

Natural variant

182

1

V → A in allele A*01:06.

VAR_016725

Natural variant

205

1

R → H.
Corresponds to variant rs17185861 [ dbSNP | Ensembl ].

VAR_056253

Secondary structure

1

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365

Helix Strand Turn

Details...

Beta strand

27 – 38

12

Turn

39 – 41

3

Beta strand

42 – 52

11

Beta strand

55 – 61

7

Beta strand

70 – 73

4

Helix

74 – 78

5

Helix

81 – 108

28

Beta strand

113 – 115

3

Beta strand

118 – 127

10

Beta strand

133 – 142

10

Beta strand

145 – 150

6

Beta strand

157 – 161

5

Helix

162 – 173

12

Helix

176 – 185

10

Helix

187 – 198

12

Helix

200 – 203

4

Beta strand

210 – 217

8

Beta strand

219 – 235

17

Beta strand

238 – 243

6

Beta strand

250 – 254

5

Beta strand

261 – 263

3

Beta strand

265 – 274

10

Helix

278 – 280

3

Beta strand

281 – 286

6

Beta strand

294 – 297

4

Sequences

	Sequence		Length	Mass (Da)
	P30443 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 8667AFF3F06C4932	FASTA	365	40,846
	10 20 30 40 50 60 MAVMAPRTLL LLLSGALALT QTWAGSHSMR YFFTSVSRPG RGEPRFIAVG YVDDTQFVRF 70 80 90 100 110 120 DSDAASQKME PRAPWIEQEG PEYWDQETRN MKAHSQTDRA NLGTLRGYYN QSEDGSHTIQ 130 140 150 160 170 180 IMYGCDVGPD GRFLRGYRQD AYDGKDYIAL NEDLRSWTAA DMAAQITKRK WEAVHAAEQR 190 200 210 220 230 240 RVYLEGRCVD GLRRYLENGK ETLQRTDPPK THMTHHPISD HEATLRCWAL GFYPAEITLT 250 260 270 280 290 300 WQRDGEDQTQ DTELVETRPA GDGTFQKWAA VVVPSGEEQR YTCHVQHEGL PKPLTLRWEL 310 320 330 340 350 360 SSQPTIPIVG IIAGLVLLGA VITGAVVAAV MWRRKSSDRK GGSYTQAASS DSAQGSDVSL TACKV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nature of polymorphism in HLA-A, -B, and -C molecules." Parham P., Lomen C.E., Lawlor D.A., Ways J.P., Holmes N., Coppin H.L., Salter R.D., Wan A.M., Ennis P.D. Proc. Natl. Acad. Sci. U.S.A. 85:4005-4009(1988) [PubMed: 3375250] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (ALLELE A*01:01).
[2]	"Diversity and diversification of HLA-A,B,C alleles." Parham P., Lawlor D.A., Lomen C.E., Ennis P.D. J. Immunol. 142:3937-3950(1989) [PubMed: 2715640] [Abstract] Cited for: NUCLEOTIDE SEQUENCE (ALLELE A*01:01).
[3]	Warren E. Submitted (APR-1989) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE (ALLELE A*01:01).
[4]	"Nucleotide sequence of an HLA-A1 gene." Girdlestone J. Nucleic Acids Res. 18:6701-6701(1990) [PubMed: 2251137] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ALLELE A*01:01).
[5]	"A nucleotide insertion in exon 4 is responsible for the absence of expression of an HLA-A*01 allele." Laforet M., Froelich N., Parissiadis A., Pfeiffer B., Schell A., Faller B., Woehl-Jaegle M.L., Cazenave J.-P., Tongio M.M. Tissue Antigens 50:347-350(1997) [PubMed: 9349617] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ALLELE A*01:01).
[6]	Waller M.J., Robinson J., Marsh S.G.E. Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE (ALLELE A*01:01).
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ALLELE A*01:01). Tissue: Brain.
[8]	"The HLA-A,B,C genotype of the class I negative cell line Daudi reveals novel HLA-A and -B alleles." Browning M.J., Madrigal J.A., Krausa P., Kowalski H., Allsopp C.E., Little A.-M., Turner S., Adams E.J., Arnett K.L., Bodmer W.F., Parham P. Tissue Antigens 45:177-187(1995) [PubMed: 7761977] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ALLELE A*01:02).
[9]	"A generic sequencing based typing approach for the identification of HLA-A diversity." Sitha S., Scheltinga S.A., Johnston-Dow L.A., White C.B., der van Zwan A.W., Bakema J.E., Rozemuller E.H., van der Tweel J.G., Kronink M.N., Tilanus M.G.J. Hum. Immunol. 57:120-128(1997) [PubMed: 9438203] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 26-206 (ALLELE A*01:03).
[10]	"High frequency of HLA-A*0103 allele in a Somali population." Poland G.A., Sohni Y., Domanico M., Kroning C.M., DeGoey S.R., Jimale M., Jacobson R.M., Moore S.B. Hum. Immunol. 62:197-200(2001) [PubMed: 11182232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 26-206 (ALLELE A*01:03). Tissue: Blood.
[11]	"Seventeen more novel HLA-A locus alleles." Ellis J., Steiner N.K., Kosman C., Henson V., Mitton W., Koester R., Ng J., Hartzman R.J., Hurley C.K. Tissue Antigens 55:369-373(2000) [PubMed: 10852390] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 26-206 (ALLELE A*01:06).
[12]	"A new HLA-A*01 allele." Tamouza R., Fortier C., Mahfoudh N., Schaeffer V., Poirier J.C., Marzais F., Gautreau C., Charron D. Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE OF 26-206 (ALLELE A*01:07).
[13]	"Human Ia alpha- and beta-chains are sulfated." Sant A.J., Zacheis M., Rumbarger T., Giacoletto K.S., Schwartz B.D. J. Immunol. 140:155-160(1988) [PubMed: 3121736] [Abstract] Cited for: SULFATION.
[14]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed: 19159218] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-110, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M24043 Genomic DNA. Translation: AAA59652.1.
X55710 Genomic DNA. Translation: CAA39243.1.
Z93949 Genomic DNA. Translation: CAB07989.1.
AJ278305 Genomic DNA. Translation: CAB93537.1.
BC003069 mRNA. Translation: AAH03069.1.
U07161 mRNA. Translation: AAA80569.1.
Y12469 Genomic DNA. Translation: CAA73072.1.
Y12470 Genomic DNA. Translation: CAA73073.1.
AF214112, AF214111 Genomic DNA. Translation: AAF19525.1.
AF143232, AF143231 Genomic DNA. Translation: AAD33894.1.
AF219633, AF219632 Genomic DNA. Translation: AAF73862.1.

IPI

IPI00026569.

PIR

I38518.
I61856.

RefSeq

NP_001229687.1. NM_001242758.1.

UniGene

Hs.181244.
Hs.713441.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1W72	X-ray	2.15	A/D	25-298	[»]
3BO8	X-ray	1.80	A	25-298	[»]

ProteinModelPortal

P30443.

SMR

P30443. Positions 25-298.

ModBase

Search...

Protein-protein interaction databases

MINT

MINT-4655826.

STRING

P30443.

Polymorphism databases

DMDM

231347.

Proteomic databases

PRIDE

P30443.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000431795; ENSP00000396220; ENSG00000229215.
ENST00000431930; ENSP00000406366; ENSG00000229215.
ENST00000443552; ENSP00000404678; ENSG00000224320.
ENST00000448357; ENSP00000402461; ENSG00000224320.

GeneID

3105.

KEGG

hsa:3105.

Organism-specific databases

CTD

3105.

GeneCards

GC06P029921.

H-InvDB

HIX0165925.
HIX0166103.

HGNC

HGNC:4931. HLA-A.

MIM

142800. gene.

neXtProt

NX_P30443.

GenAtlas

Search...

Phylogenomic databases

HOVERGEN

HBG016709.

OrthoDB

EOG4F4SBG.

Enzyme and pathway databases

Reactome

REACT_6900. Immune System.

Gene expression databases

CleanEx

HS_HLA-A.

Genevestigator

P30443.

Family and domain databases

InterPro

IPR007110. Ig-like.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
IPR011161. MHC_I-like_Ag-recog.
IPR011162. MHC_I/II-like_Ag-recog.
IPR001039. MHC_I_a_a1/a2.
IPR010579. MHC_I_a_C.
[Graphical view]

Gene3D

G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
G3DSA:3.30.500.10. MHC_I-like_Ag-recog. 1 hit.

KO

K06751.

Pfam

PF07654. C1-set. 1 hit.
PF00129. MHC_I. 1 hit.
PF06623. MHC_I_C. 1 hit.
[Graphical view]

PRINTS

PR01638. MHCCLASSI.

SMART

SM00407. IGc1. 1 hit.
[Graphical view]

SUPFAM

SSF54452. MHC_I/II-like_Ag-recog. 1 hit.

PROSITE

PS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

SOURCE

Search...

Entry information

Entry name

1A01_HUMAN

Accession

Primary (citable) accession number: P30443
Secondary accession number(s): O77964 , O78171, Q9MYA3, Q9TP25, Q9TQP5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	January 25, 2012
This is version 109 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.