Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P30443 (1A01_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
HLA class I histocompatibility antigen, A-1 alpha chain
Alternative name(s):
MHC class I antigen A*1
Gene names
Name:HLA-A
Synonyms:HLAA
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length365 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the presentation of foreign antigens to the immune system.

Subunit structure

Dimer of alpha chain and a beta chain (beta-2-microglobulin). Interacts with human herpesvirus 8 MIR1 protein By similarity.

Subcellular location

Membrane; Single-pass type I membrane protein.

Post-translational modification

Sulfated.

Polyubiquitinated in a post ER compartment through interaction with human herpesvirus 8 MIR1 protein. This targets the protein for rapid degradation via the ubiquitin system By similarity.

Polymorphism

The following alleles of A-1 are known: A*01:01, A*01:02, A*01:03, A*01:06 and A*01:07. The sequence shown is that of A*01:01.

Sequence similarities

Belongs to the MHC class I family.

Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

Ontologies

Keywords
   Biological processHost-virus interaction
Immunity
   Cellular componentMembrane
MHC I
   Coding sequence diversityPolymorphism
   DomainImmunoglobulin domain
Signal
Transmembrane
Transmembrane helix
   PTMDisulfide bond
Glycoprotein
Sulfation
Ubl conjugation
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processantigen processing and presentation of exogenous peptide antigen via MHC class I

Traceable author statement. Source: Reactome

antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent

Traceable author statement. Source: Reactome

antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent

Traceable author statement. Source: Reactome

antigen processing and presentation of peptide antigen via MHC class I

Traceable author statement. Source: Reactome

cytokine-mediated signaling pathway

Traceable author statement. Source: Reactome

detection of bacterium

Inferred from mutant phenotype PubMed 22321387. Source: UniProtKB

immune response

Inferred from mutant phenotype PubMed 22321387. Source: UniProtKB

interferon-gamma-mediated signaling pathway

Traceable author statement. Source: Reactome

positive regulation of T cell mediated cytotoxicity

Inferred from direct assay PubMed 2420472. Source: UniProt

regulation of immune response

Traceable author statement. Source: Reactome

type I interferon signaling pathway

Traceable author statement. Source: Reactome

viral process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentER to Golgi transport vesicle membrane

Traceable author statement. Source: Reactome

Golgi membrane

Traceable author statement. Source: Reactome

MHC class I protein complex

Inferred from electronic annotation. Source: UniProtKB-KW

early endosome membrane

Traceable author statement. Source: Reactome

extracellular vesicular exosome

Inferred from direct assay PubMed 19199708. Source: UniProt

integral component of lumenal side of endoplasmic reticulum membrane

Traceable author statement. Source: Reactome

integral component of plasma membrane

Non-traceable author statement Ref.2. Source: UniProtKB

phagocytic vesicle membrane

Traceable author statement. Source: Reactome

plasma membrane

Traceable author statement. Source: Reactome

   Molecular_functionTAP binding

Inferred from direct assay PubMed 8617941. Source: UniProt

peptide antigen binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

receptor binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424
Chain25 – 365341HLA class I histocompatibility antigen, A-1 alpha chain
PRO_0000018813

Regions

Topological domain25 – 308284Extracellular Potential
Transmembrane309 – 33224Helical; Potential
Topological domain333 – 36533Cytoplasmic Potential
Domain209 – 29587Ig-like C1-type
Region25 – 11490Alpha-1
Region115 – 20692Alpha-2
Region207 – 29892Alpha-3
Region299 – 30810Connecting peptide

Amino acid modifications

Modified residue831Sulfotyrosine Potential
Glycosylation1101N-linked (GlcNAc...) Ref.14
Disulfide bond125 ↔ 188 Ref.15
Disulfide bond227 ↔ 283 Ref.15

Natural variations

Natural variant331F → S in allele A*01:02.
Corresponds to variant rs2075684 [ dbSNP | Ensembl ].
VAR_004332
Natural variant411R → S in allele A*01:02.
VAR_004333
Natural variant801G → R in allele A*01:07.
VAR_016719
Natural variant891R → G.
Corresponds to variant rs1059459 [ dbSNP | Ensembl ].
VAR_056247
Natural variant911M → V in allele A*01:07.
VAR_016720
Natural variant1001A → E in allele A*01:07.
VAR_016721
Natural variant1141D → A in allele A*01:07.
VAR_016722
Natural variant1211I → M in allele A*01:03.
VAR_016723
Natural variant1311G → W.
Corresponds to variant rs1136702 [ dbSNP | Ensembl ].
VAR_056248
Natural variant1331F → L.
Corresponds to variant rs1059488 [ dbSNP | Ensembl ].
VAR_056249
Natural variant1511N → K.
Corresponds to variant rs1059509 [ dbSNP | Ensembl ].
VAR_056250
Natural variant1661I → T. Ref.16
Corresponds to variant rs1059516 [ dbSNP | Ensembl ].
VAR_056251
Natural variant1691R → H.
Corresponds to variant rs1059520 [ dbSNP | Ensembl ].
VAR_056252
Natural variant1801R → L in allele A*01:06.
VAR_016724
Natural variant1821V → A in allele A*01:06.
VAR_016725
Natural variant2051R → H.
Corresponds to variant rs17185861 [ dbSNP | Ensembl ].
VAR_056253

Secondary structure

....................................................... 365
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P30443 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: 8667AFF3F06C4932

FASTA36540,846
        10         20         30         40         50         60 
MAVMAPRTLL LLLSGALALT QTWAGSHSMR YFFTSVSRPG RGEPRFIAVG YVDDTQFVRF 

        70         80         90        100        110        120 
DSDAASQKME PRAPWIEQEG PEYWDQETRN MKAHSQTDRA NLGTLRGYYN QSEDGSHTIQ 

       130        140        150        160        170        180 
IMYGCDVGPD GRFLRGYRQD AYDGKDYIAL NEDLRSWTAA DMAAQITKRK WEAVHAAEQR 

       190        200        210        220        230        240 
RVYLEGRCVD GLRRYLENGK ETLQRTDPPK THMTHHPISD HEATLRCWAL GFYPAEITLT 

       250        260        270        280        290        300 
WQRDGEDQTQ DTELVETRPA GDGTFQKWAA VVVPSGEEQR YTCHVQHEGL PKPLTLRWEL 

       310        320        330        340        350        360 
SSQPTIPIVG IIAGLVLLGA VITGAVVAAV MWRRKSSDRK GGSYTQAASS DSAQGSDVSL 


TACKV 

« Hide

References

« Hide 'large scale' references
[1]"Nature of polymorphism in HLA-A, -B, and -C molecules."
Parham P., Lomen C.E., Lawlor D.A., Ways J.P., Holmes N., Coppin H.L., Salter R.D., Wan A.M., Ennis P.D.
Proc. Natl. Acad. Sci. U.S.A. 85:4005-4009(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE (ALLELE A*01:01).
[2]"Diversity and diversification of HLA-A,B,C alleles."
Parham P., Lawlor D.A., Lomen C.E., Ennis P.D.
J. Immunol. 142:3937-3950(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE (ALLELE A*01:01).
[3]Warren E.
Submitted (APR-1989) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE (ALLELE A*01:01).
[4]"Nucleotide sequence of an HLA-A1 gene."
Girdlestone J.
Nucleic Acids Res. 18:6701-6701(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ALLELE A*01:01).
[5]"A nucleotide insertion in exon 4 is responsible for the absence of expression of an HLA-A*01 allele."
Laforet M., Froelich N., Parissiadis A., Pfeiffer B., Schell A., Faller B., Woehl-Jaegle M.L., Cazenave J.-P., Tongio M.M.
Tissue Antigens 50:347-350(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ALLELE A*01:01).
[6]Waller M.J., Robinson J., Marsh S.G.E.
Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE (ALLELE A*01:01).
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ALLELE A*01:01).
Tissue: Brain.
[8]"The HLA-A,B,C genotype of the class I negative cell line Daudi reveals novel HLA-A and -B alleles."
Browning M.J., Madrigal J.A., Krausa P., Kowalski H., Allsopp C.E., Little A.-M., Turner S., Adams E.J., Arnett K.L., Bodmer W.F., Parham P.
Tissue Antigens 45:177-187(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ALLELE A*01:02).
[9]"A generic sequencing based typing approach for the identification of HLA-A diversity."
Sitha S., Scheltinga S.A., Johnston-Dow L.A., White C.B., der van Zwan A.W., Bakema J.E., Rozemuller E.H., van der Tweel J.G., Kronink M.N., Tilanus M.G.J.
Hum. Immunol. 57:120-128(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE OF 26-206 (ALLELE A*01:03).
[10]"High frequency of HLA-A*0103 allele in a Somali population."
Poland G.A., Sohni Y., Domanico M., Kroning C.M., DeGoey S.R., Jimale M., Jacobson R.M., Moore S.B.
Hum. Immunol. 62:197-200(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE OF 26-206 (ALLELE A*01:03).
Tissue: Blood.
[11]"Seventeen more novel HLA-A locus alleles."
Ellis J., Steiner N.K., Kosman C., Henson V., Mitton W., Koester R., Ng J., Hartzman R.J., Hurley C.K.
Tissue Antigens 55:369-373(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE OF 26-206 (ALLELE A*01:06).
[12]"A new HLA-A*01 allele."
Tamouza R., Fortier C., Mahfoudh N., Schaeffer V., Poirier J.C., Marzais F., Gautreau C., Charron D.
Submitted (DEC-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE OF 26-206 (ALLELE A*01:07).
[13]"Human Ia alpha- and beta-chains are sulfated."
Sant A.J., Zacheis M., Rumbarger T., Giacoletto K.S., Schwartz B.D.
J. Immunol. 140:155-160(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: SULFATION.
[14]"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-110.
Tissue: Liver.
[15]"A major histocompatibility complex-peptide-restricted antibody and t cell receptor molecules recognize their target by distinct binding modes: crystal structure of human leukocyte antigen (HLA)-A1-MAGE-A1 in complex with FAB-HYB3."
Hulsmeyer M., Chames P., Hillig R.C., Stanfield R.L., Held G., Coulie P.G., Alings C., Wille G., Saenger W., Uchanska-Ziegler B., Hoogenboom H.R., Ziegler A.
J. Biol. Chem. 280:2972-2980(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS) OF 25-298 IN COMPLEX WITH MAGE ANTIGENIC PEPTIDE AND ANTIBODY, DISULFIDE BONDS.
[16]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANT [LARGE SCALE ANALYSIS] THR-166, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M24043 Genomic DNA. Translation: AAA59652.1.
X55710 Genomic DNA. Translation: CAA39243.1.
Z93949 Genomic DNA. Translation: CAB07989.1.
AJ278305 Genomic DNA. Translation: CAB93537.1.
BC003069 mRNA. Translation: AAH03069.1.
U07161 mRNA. Translation: AAA80569.1.
Y12469 Genomic DNA. Translation: CAA73072.1.
Y12470 Genomic DNA. Translation: CAA73073.1.
AH008863 Genomic DNA. Translation: AAF19525.1.
AH007762 Genomic DNA. Translation: AAD33894.1.
AH009407 Genomic DNA. Translation: AAF73862.1.
PIRI38518.
I61856.
RefSeqNP_001229687.1. NM_001242758.1.
UniGeneHs.181244.
Hs.713441.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1W72X-ray2.15A/D25-298[»]
3BO8X-ray1.80A25-298[»]
4NQVX-ray2.39A/C/E/G/I/K25-298[»]
4NQXX-ray2.00A/C/E/G/I/K25-308[»]
ProteinModelPortalP30443.
SMRP30443. Positions 25-298.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid109350. 31 interactions.
IntActP30443. 1 interaction.
MINTMINT-4655826.

Polymorphism databases

DMDM231347.

Proteomic databases

PaxDbP30443.
PRIDEP30443.

Protocols and materials databases

DNASU3105.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000431930; ENSP00000406366; ENSG00000229215.
ENST00000443552; ENSP00000404678; ENSG00000224320.
ENST00000549869; ENSP00000447635; ENSG00000224320.
ENST00000552193; ENSP00000447614; ENSG00000229215.
GeneID3105.
KEGGhsa:3105.
UCSCuc011ejp.2. human.

Organism-specific databases

CTD3105.
GeneCardsGC06P029949.
GC06Pi29846.
GC06Pj29898.
HGNCHGNC:4931. HLA-A.
MIM142800. gene.
neXtProtNX_P30443.
Orphanet179. Chorioretinopathy, Birdshot type.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG42056.
HOVERGENHBG016709.
KOK06751.

Enzyme and pathway databases

ReactomeREACT_6900. Immune System.

Gene expression databases

CleanExHS_HLA-A.
GenevestigatorP30443.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
3.30.500.10. 1 hit.
InterProIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR003006. Ig/MHC_CS.
IPR003597. Ig_C1-set.
IPR011161. MHC_I-like_Ag-recog.
IPR011162. MHC_I/II-like_Ag-recog.
IPR027648. MHC_I_a.
IPR001039. MHC_I_a_a1/a2.
IPR010579. MHC_I_a_C.
[Graphical view]
PfamPF07654. C1-set. 1 hit.
PF00129. MHC_I. 1 hit.
PF06623. MHC_I_C. 1 hit.
[Graphical view]
PRINTSPR01638. MHCCLASSI.
SMARTSM00407. IGc1. 1 hit.
[Graphical view]
SUPFAMSSF54452. SSF54452. 1 hit.
PROSITEPS50835. IG_LIKE. 1 hit.
PS00290. IG_MHC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSHLA-A. human.
EvolutionaryTraceP30443.
GenomeRNAi3105.
NextBio12319.
SOURCESearch...

Entry information

Entry name1A01_HUMAN
AccessionPrimary (citable) accession number: P30443
Secondary accession number(s): O77964 expand/collapse secondary AC list , O78171, Q9MYA3, Q9TP25, Q9TQP5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: April 16, 2014
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 6

Human chromosome 6: entries, gene names and cross-references to MIM