ID   FEL1B_FELCA             Reviewed;         109 AA.
AC   P30440;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1993, sequence version 1.
DT   24-JAN-2024, entry version 123.
DE   RecName: Full=Major allergen I polypeptide chain 2;
DE   AltName: Full=AG4;
DE   AltName: Full=Allergen Cat-1;
DE   AltName: Full=Allergen Fel d I-B;
DE            Short=Allergen FdI;
DE   AltName: Allergen=Fel d 1-B;
DE   Flags: Precursor;
GN   Name=CH2;
OS   Felis catus (Cat) (Felis silvestris catus).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Carnivora; Feliformia; Felidae; Felinae; Felis.
OX   NCBI_TaxID=9685;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 18-100.
RX   PubMed=1946388; DOI=10.1073/pnas.88.21.9690;
RA   Morgenstern J.P., Griffith I.J., Brauer A.W., Rogers B.L., Bond J.F.,
RA   Chapman M.D., Kuo M.-C.;
RT   "Amino acid sequence of Fel dI, the major allergen of the domestic cat:
RT   protein sequence analysis and cDNA cloning.";
RL   Proc. Natl. Acad. Sci. U.S.A. 88:9690-9694(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE, ALTERNATIVE SPLICING, AND VARIANTS.
RC   TISSUE=Liver;
RX   PubMed=1572548; DOI=10.1016/0378-1119(92)90405-e;
RA   Griffith I.J., Craig S., Pollock J., Yu X.-B., Morgenstern J.P.,
RA   Rogers B.L.;
RT   "Expression and genomic structure of the genes encoding FdI, the major
RT   allergen from the domestic cat.";
RL   Gene 113:263-268(1992).
RN   [3]
RP   PROTEIN SEQUENCE OF 18-37, AND CHARACTERIZATION.
RX   PubMed=1712068; DOI=10.1016/0161-5890(91)90141-6;
RA   Duffort O.A., Carreira J., Nitti G., Polo F., Lombardero M.;
RT   "Studies on the biochemical structure of the major cat allergen Felis
RT   domesticus I.";
RL   Mol. Immunol. 28:301-309(1991).
RN   [4]
RP   CHARACTERIZATION.
RX   PubMed=6747135; DOI=10.1016/0091-6749(84)90278-1;
RA   Leitermann K., Ohman J.L. Jr.;
RT   "Cat allergen 1: biochemical, antigenic, and allergenic properties.";
RL   J. Allergy Clin. Immunol. 74:147-153(1984).
RN   [5]
RP   X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) OF 18-109.
RX   PubMed=12851385; DOI=10.1074/jbc.m304740200;
RA   Kaiser L., Gronlund H., Sandalova T., Ljunggren H.G., van Hage-Hamsten M.,
RA   Achour A., Schneider G.;
RT   "The crystal structure of the major cat allergen Fel d 1, a member of the
RT   secretoglobin family.";
RL   J. Biol. Chem. 278:37730-37735(2003).
CC   -!- SUBUNIT: Heterotetramer composed of two non-covalently linked
CC       disulfide-linked heterodimer of chains 1 and 2.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=3;
CC         Comment=Experimental confirmation may be lacking for some isoforms.;
CC       Name=1; Synonyms=CH2L;
CC         IsoId=P30440-1; Sequence=Displayed;
CC       Name=2; Synonyms=CH2S;
CC         IsoId=P30440-2; Sequence=VSP_004249;
CC       Name=3; Synonyms=CH2ST, Truncated;
CC         IsoId=P30440-3; Sequence=VSP_004248;
CC   -!- TISSUE SPECIFICITY: The long form is preferentially expressed in the
CC       salivary gland, while the short form is preferentially expressed in the
CC       skin.
CC   -!- ALLERGEN: Causes an allergic reaction in human. Binds to IgE. Major
CC       allergen produced by the domestic cat. Implicated as an asthma-inducing
CC       agent in human. This protein is sticky and easily adheres to walls,
CC       carpet, clothing, furniture and bedding.
CC   -!- SIMILARITY: Belongs to the secretoglobin family. {ECO:0000305}.
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DR   EMBL; M77341; AAC41616.1; -; mRNA.
DR   EMBL; X62478; CAA44345.1; -; Genomic_DNA.
DR   PIR; B53283; B53283.
DR   PIR; C56413; C56413.
DR   PIR; JC1127; JC1127.
DR   RefSeq; NP_001041619.1; NM_001048154.1. [P30440-1]
DR   PDB; 1PUO; X-ray; 1.85 A; A/B=18-109.
DR   PDB; 1ZKR; X-ray; 1.64 A; A/B=18-93.
DR   PDB; 2EJN; X-ray; 1.64 A; A/B=18-93.
DR   PDB; 5VYF; X-ray; 2.90 A; C/F=18-109.
DR   PDBsum; 1PUO; -.
DR   PDBsum; 1ZKR; -.
DR   PDBsum; 2EJN; -.
DR   PDBsum; 5VYF; -.
DR   AlphaFoldDB; P30440; -.
DR   SMR; P30440; -.
DR   Allergome; 3278; Fel d 1.0101.
DR   Allergome; 345; Fel d 1.
DR   GlyCosmos; P30440; 1 site, No reported glycans.
DR   PaxDb; 9685-ENSFCAP00000019678; -.
DR   ABCD; P30440; 4 sequenced antibodies.
DR   GeneID; 677879; -.
DR   KEGG; fca:677879; -.
DR   CTD; 677879; -.
DR   eggNOG; ENOG502RU0W; Eukaryota.
DR   InParanoid; P30440; -.
DR   OrthoDB; 4988315at2759; -.
DR   EvolutionaryTrace; P30440; -.
DR   Proteomes; UP000011712; Unplaced.
DR   GO; GO:0005615; C:extracellular space; IEA:InterPro.
DR   CDD; cd00633; Secretoglobin; 1.
DR   Gene3D; 1.20.920.50; -; 1.
DR   InterPro; IPR015332; CH2-like.
DR   InterPro; IPR016126; Secretoglobin.
DR   InterPro; IPR035960; Secretoglobin_sf.
DR   PANTHER; PTHR31708; ABPBG26-RELATED; 1.
DR   PANTHER; PTHR31708:SF0; ABPBG26-RELATED; 1.
DR   Pfam; PF09252; Feld-I_B; 1.
DR   SUPFAM; SSF48201; Uteroglobin-like; 1.
DR   PROSITE; PS51311; SCGB; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Allergen; Alternative splicing; Direct protein sequencing;
KW   Disulfide bond; Glycoprotein; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..17
FT                   /evidence="ECO:0000269|PubMed:1712068,
FT                   ECO:0000269|PubMed:1946388"
FT   CHAIN           18..109
FT                   /note="Major allergen I polypeptide chain 2"
FT                   /id="PRO_0000021248"
FT   CARBOHYD        50
FT                   /note="N-linked (GlcNAc...) asparagine"
FT   DISULFID        24
FT                   /note="Interchain (with C-92 in chain 1)"
FT   DISULFID        65
FT                   /note="Interchain (with C-66 in chain 1)"
FT   DISULFID        90
FT                   /note="Interchain (with C-25 in chain 1)"
FT   VAR_SEQ         82..109
FT                   /note="TTISSSKDCMGEAVQNTVEDLKLNTLGR -> PSTNIAWVKQFRTP (in
FT                   isoform 3)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_004248"
FT   VAR_SEQ         82..89
FT                   /note="TTISSSKD -> IAINEY (in isoform 2)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_004249"
FT   VARIANT         72
FT                   /note="I -> L (in CH2LV)"
FT   VARIANT         72
FT                   /note="I -> V (in CH2SV)"
FT   VARIANT         74..75
FT                   /note="RV -> KF (in CH2SV)"
FT   VARIANT         91
FT                   /note="M -> T (in CH2LV)"
FT   VARIANT         96
FT                   /note="Q -> E (in CH2SV)"
FT   VARIANT         105
FT                   /note="N -> K (in CH2SV)"
FT   CONFLICT        24
FT                   /note="C -> F (in Ref. 3; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        32
FT                   /note="F -> T (in Ref. 3; AA sequence)"
FT                   /evidence="ECO:0000305"
FT   STRAND          19..23
FT                   /evidence="ECO:0007829|PDB:1ZKR"
FT   HELIX           25..35
FT                   /evidence="ECO:0007829|PDB:1ZKR"
FT   HELIX           39..48
FT                   /evidence="ECO:0007829|PDB:1ZKR"
FT   HELIX           53..68
FT                   /evidence="ECO:0007829|PDB:1ZKR"
FT   HELIX           71..73
FT                   /evidence="ECO:0007829|PDB:1ZKR"
FT   STRAND          74..76
FT                   /evidence="ECO:0007829|PDB:1ZKR"
FT   HELIX           77..85
FT                   /evidence="ECO:0007829|PDB:1ZKR"
FT   TURN            88..90
FT                   /evidence="ECO:0007829|PDB:1ZKR"
SQ   SEQUENCE   109 AA;  11854 MW;  857FB9CD76036CB9 CRC64;
     MRGALLVLAL LVTQALGVKM AETCPIFYDV FFAVANGNEL LLDLSLTKVN ATEPERTAMK
     KIQDCYVENG LISRVLDGLV MTTISSSKDC MGEAVQNTVE DLKLNTLGR
//