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Protein

Major allergen I polypeptide chain 2

Gene

CH2

Organism
Felis catus (Cat) (Felis silvestris catus)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Names & Taxonomyi

Protein namesi
Recommended name:
Major allergen I polypeptide chain 2
Alternative name(s):
AG4
Allergen Cat-1
Allergen Fel d I-B
Short name:
Allergen FdI
Allergen: Fel d 1-B
Gene namesi
Name:CH2
OrganismiFelis catus (Cat) (Felis silvestris catus)
Taxonomic identifieri9685 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraFeliformiaFelidaeFelinaeFelis
Proteomesi
  • UP000011712 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. Binds to IgE. Major allergen produced by the domestic cat. Implicated as an asthma-inducing agent in human. This protein is sticky and easily adheres to walls, carpet, clothing, furniture and bedding.

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei3278. Fel d 1.0101.
345. Fel d 1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 172 PublicationsAdd BLAST17
ChainiPRO_000002124818 – 109Major allergen I polypeptide chain 2Add BLAST92

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi24Interchain (with C-92 in chain 1)
Glycosylationi50N-linked (GlcNAc...)1
Disulfide bondi65Interchain (with C-66 in chain 1)
Disulfide bondi90Interchain (with C-25 in chain 1)

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

The long form is preferentially expressed in the salivary gland, while the short form is preferentially expressed in the skin.

Interactioni

Subunit structurei

Heterotetramer composed of two non-covalently linked disulfide-linked heterodimer of chains 1 and 2.

Protein-protein interaction databases

STRINGi9685.ENSFCAP00000019678.

Structurei

Secondary structure

1109
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi19 – 23Combined sources5
Helixi25 – 35Combined sources11
Helixi39 – 48Combined sources10
Helixi53 – 68Combined sources16
Helixi71 – 73Combined sources3
Beta strandi74 – 76Combined sources3
Helixi77 – 85Combined sources9
Turni88 – 90Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PUOX-ray1.85A/B18-109[»]
1ZKRX-ray1.64A/B18-93[»]
2EJNX-ray1.64A/B18-93[»]
ProteinModelPortaliP30440.
SMRiP30440.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP30440.

Family & Domainsi

Sequence similaritiesi

Belongs to the secretoglobin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410JIV4. Eukaryota.
ENOG41114U0. LUCA.
HOVERGENiHBG063446.
InParanoidiP30440.

Family and domain databases

CDDicd00633. Secretoglobin. 1 hit.
InterProiIPR015332. Allergen_Fel_d_1_chain2.
IPR016126. Secretoglobin.
[Graphical view]
PfamiPF09252. Feld-I_B. 1 hit.
[Graphical view]
SUPFAMiSSF48201. SSF48201. 1 hit.
PROSITEiPS51311. SCGB. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Experimental confirmation may be lacking for some isoforms.
Isoform 1 (identifier: P30440-1) [UniParc]FASTAAdd to basket
Also known as: CH2L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRGALLVLAL LVTQALGVKM AETCPIFYDV FFAVANGNEL LLDLSLTKVN
60 70 80 90 100
ATEPERTAMK KIQDCYVENG LISRVLDGLV MTTISSSKDC MGEAVQNTVE

DLKLNTLGR
Length:109
Mass (Da):11,854
Last modified:April 1, 1993 - v1
Checksum:i857FB9CD76036CB9
GO
Isoform 2 (identifier: P30440-2) [UniParc]FASTAAdd to basket
Also known as: CH2S

The sequence of this isoform differs from the canonical sequence as follows:
     82-89: TTISSSKD → IAINEY

Show »
Length:107
Mass (Da):11,738
Checksum:i3D8AD80A8251E0B2
GO
Isoform 3 (identifier: P30440-3) [UniParc]FASTAAdd to basket
Also known as: CH2ST, Truncated

The sequence of this isoform differs from the canonical sequence as follows:
     82-109: TTISSSKDCMGEAVQNTVEDLKLNTLGR → PSTNIAWVKQFRTP

Show »
Length:95
Mass (Da):10,487
Checksum:i0E5448722934E6E2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti24C → F AA sequence (PubMed:1712068).Curated1
Sequence conflicti32F → T AA sequence (PubMed:1712068).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti72I → L in CH2LV. 1
Natural varianti72I → V in CH2SV. 1
Natural varianti74 – 75RV → KF in CH2SV. 2
Natural varianti91M → T in CH2LV. 1
Natural varianti96Q → E in CH2SV. 1
Natural varianti105N → K in CH2SV. 1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00424882 – 109TTISS…NTLGR → PSTNIAWVKQFRTP in isoform 3. CuratedAdd BLAST28
Alternative sequenceiVSP_00424982 – 89TTISSSKD → IAINEY in isoform 2. Curated8

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M77341 mRNA. Translation: AAC41616.1.
X62478 Genomic DNA. Translation: CAA44345.1.
PIRiB53283.
C56413.
JC1127.
RefSeqiNP_001041619.1. NM_001048154.1. [P30440-1]

Genome annotation databases

GeneIDi677879.
KEGGifca:677879.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M77341 mRNA. Translation: AAC41616.1.
X62478 Genomic DNA. Translation: CAA44345.1.
PIRiB53283.
C56413.
JC1127.
RefSeqiNP_001041619.1. NM_001048154.1. [P30440-1]

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PUOX-ray1.85A/B18-109[»]
1ZKRX-ray1.64A/B18-93[»]
2EJNX-ray1.64A/B18-93[»]
ProteinModelPortaliP30440.
SMRiP30440.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9685.ENSFCAP00000019678.

Protein family/group databases

Allergomei3278. Fel d 1.0101.
345. Fel d 1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi677879.
KEGGifca:677879.

Organism-specific databases

CTDi677879.

Phylogenomic databases

eggNOGiENOG410JIV4. Eukaryota.
ENOG41114U0. LUCA.
HOVERGENiHBG063446.
InParanoidiP30440.

Miscellaneous databases

EvolutionaryTraceiP30440.

Family and domain databases

CDDicd00633. Secretoglobin. 1 hit.
InterProiIPR015332. Allergen_Fel_d_1_chain2.
IPR016126. Secretoglobin.
[Graphical view]
PfamiPF09252. Feld-I_B. 1 hit.
[Graphical view]
SUPFAMiSSF48201. SSF48201. 1 hit.
PROSITEiPS51311. SCGB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFEL1B_FELCA
AccessioniPrimary (citable) accession number: P30440
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: November 30, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.