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P30440

- FEL1B_FELCA

UniProt

P30440 - FEL1B_FELCA

Protein

Major allergen I polypeptide chain 2

Gene

CH2

Organism
Felis catus (Cat) (Felis silvestris catus)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Names & Taxonomyi

    Protein namesi
    Recommended name:
    Major allergen I polypeptide chain 2
    Alternative name(s):
    AG4
    Allergen Cat-1
    Allergen Fel d I-B
    Short name:
    Allergen FdI
    Allergen: Fel d 1-B
    Gene namesi
    Name:CH2
    OrganismiFelis catus (Cat) (Felis silvestris catus)
    Taxonomic identifieri9685 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraFeliformiaFelidaeFelinaeFelis
    ProteomesiUP000011712: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: InterPro

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Allergenic propertiesi

    Causes an allergic reaction in human. Binds to IgE. Major allergen produced by the domestic cat. Implicated as an asthma-inducing agent in human. This protein is sticky and easily adheres to walls, carpet, clothing, furniture and bedding.

    Keywords - Diseasei

    Allergen

    Protein family/group databases

    Allergomei3278. Fel d 1.0101.
    345. Fel d 1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 17172 PublicationsAdd
    BLAST
    Chaini18 – 10992Major allergen I polypeptide chain 2PRO_0000021248Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi24 – 24Interchain (with C-92 in chain 1)
    Glycosylationi50 – 501N-linked (GlcNAc...)
    Disulfide bondi65 – 65Interchain (with C-66 in chain 1)
    Disulfide bondi90 – 90Interchain (with C-25 in chain 1)

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Expressioni

    Tissue specificityi

    The long form is preferentially expressed in the salivary gland, while the short form is preferentially expressed in the skin.

    Interactioni

    Subunit structurei

    Heterotetramer composed of two non-covalently linked disulfide-linked heterodimer of chains 1 and 2.

    Structurei

    Secondary structure

    1
    109
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi19 – 235
    Helixi25 – 3511
    Helixi39 – 4810
    Helixi53 – 6816
    Helixi71 – 733
    Beta strandi74 – 763
    Helixi77 – 859
    Turni88 – 903

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1PUOX-ray1.85A/B18-109[»]
    1ZKRX-ray1.64A/B18-93[»]
    2EJNX-ray1.64A/B18-93[»]
    ProteinModelPortaliP30440.
    SMRiP30440. Positions 22-91.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP30440.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the secretoglobin family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    HOVERGENiHBG063446.

    Family and domain databases

    InterProiIPR015332. Allergen_Fel_d_1_chain2.
    IPR016126. Secretoglobin.
    [Graphical view]
    PfamiPF09252. Feld-I_B. 1 hit.
    [Graphical view]
    SUPFAMiSSF48201. SSF48201. 1 hit.
    PROSITEiPS51311. SCGB. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Note: Experimental confirmation may be lacking for some isoforms.

    Isoform 1 (identifier: P30440-1) [UniParc]FASTAAdd to Basket

    Also known as: CH2L

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MRGALLVLAL LVTQALGVKM AETCPIFYDV FFAVANGNEL LLDLSLTKVN    50
    ATEPERTAMK KIQDCYVENG LISRVLDGLV MTTISSSKDC MGEAVQNTVE 100
    DLKLNTLGR 109
    Length:109
    Mass (Da):11,854
    Last modified:April 1, 1993 - v1
    Checksum:i857FB9CD76036CB9
    GO
    Isoform 2 (identifier: P30440-2) [UniParc]FASTAAdd to Basket

    Also known as: CH2S

    The sequence of this isoform differs from the canonical sequence as follows:
         82-89: TTISSSKD → IAINEY

    Show »
    Length:107
    Mass (Da):11,738
    Checksum:i3D8AD80A8251E0B2
    GO
    Isoform 3 (identifier: P30440-3) [UniParc]FASTAAdd to Basket

    Also known as: CH2ST, Truncated

    The sequence of this isoform differs from the canonical sequence as follows:
         82-109: TTISSSKDCMGEAVQNTVEDLKLNTLGR → PSTNIAWVKQFRTP

    Show »
    Length:95
    Mass (Da):10,487
    Checksum:i0E5448722934E6E2
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti24 – 241C → F AA sequence (PubMed:1712068)Curated
    Sequence conflicti32 – 321F → T AA sequence (PubMed:1712068)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti72 – 721I → L in CH2LV.
    Natural varianti72 – 721I → V in CH2SV.
    Natural varianti74 – 752RV → KF in CH2SV.
    Natural varianti91 – 911M → T in CH2LV.
    Natural varianti96 – 961Q → E in CH2SV.
    Natural varianti105 – 1051N → K in CH2SV.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei82 – 10928TTISS…NTLGR → PSTNIAWVKQFRTP in isoform 3. CuratedVSP_004248Add
    BLAST
    Alternative sequencei82 – 898TTISSSKD → IAINEY in isoform 2. CuratedVSP_004249

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M77341 mRNA. Translation: AAC41616.1.
    X62478 Genomic DNA. Translation: CAA44345.1.
    PIRiB53283.
    C56413.
    JC1127.
    RefSeqiNP_001041619.1. NM_001048154.1. [P30440-1]

    Genome annotation databases

    GeneIDi677879.
    KEGGifca:677879.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M77341 mRNA. Translation: AAC41616.1 .
    X62478 Genomic DNA. Translation: CAA44345.1 .
    PIRi B53283.
    C56413.
    JC1127.
    RefSeqi NP_001041619.1. NM_001048154.1. [P30440-1 ]

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1PUO X-ray 1.85 A/B 18-109 [» ]
    1ZKR X-ray 1.64 A/B 18-93 [» ]
    2EJN X-ray 1.64 A/B 18-93 [» ]
    ProteinModelPortali P30440.
    SMRi P30440. Positions 22-91.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    Allergomei 3278. Fel d 1.0101.
    345. Fel d 1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 677879.
    KEGGi fca:677879.

    Organism-specific databases

    CTDi 677879.

    Phylogenomic databases

    HOVERGENi HBG063446.

    Miscellaneous databases

    EvolutionaryTracei P30440.

    Family and domain databases

    InterProi IPR015332. Allergen_Fel_d_1_chain2.
    IPR016126. Secretoglobin.
    [Graphical view ]
    Pfami PF09252. Feld-I_B. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48201. SSF48201. 1 hit.
    PROSITEi PS51311. SCGB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Amino acid sequence of Fel dI, the major allergen of the domestic cat: protein sequence analysis and cDNA cloning."
      Morgenstern J.P., Griffith I.J., Brauer A.W., Rogers B.L., Bond J.F., Chapman M.D., Kuo M.-C.
      Proc. Natl. Acad. Sci. U.S.A. 88:9690-9694(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 18-100.
    2. "Expression and genomic structure of the genes encoding FdI, the major allergen from the domestic cat."
      Griffith I.J., Craig S., Pollock J., Yu X.-B., Morgenstern J.P., Rogers B.L.
      Gene 113:263-268(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE, ALTERNATIVE SPLICING, VARIANTS.
      Tissue: Liver.
    3. "Studies on the biochemical structure of the major cat allergen Felis domesticus I."
      Duffort O.A., Carreira J., Nitti G., Polo F., Lombardero M.
      Mol. Immunol. 28:301-309(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 18-37, CHARACTERIZATION.
    4. "Cat allergen 1: biochemical, antigenic, and allergenic properties."
      Leitermann K., Ohman J.L. Jr.
      J. Allergy Clin. Immunol. 74:147-153(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    5. "The crystal structure of the major cat allergen Fel d 1, a member of the secretoglobin family."
      Kaiser L., Gronlund H., Sandalova T., Ljunggren H.G., van Hage-Hamsten M., Achour A., Schneider G.
      J. Biol. Chem. 278:37730-37735(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) OF 18-109.

    Entry informationi

    Entry nameiFEL1B_FELCA
    AccessioniPrimary (citable) accession number: P30440
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: April 1, 1993
    Last modified: October 1, 2014
    This is version 90 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Allergens
      Nomenclature of allergens and list of entries
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3