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P30440

- FEL1B_FELCA

UniProt

P30440 - FEL1B_FELCA

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Protein

Major allergen I polypeptide chain 2

Gene

CH2

Organism
Felis catus (Cat) (Felis silvestris catus)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Names & Taxonomyi

Protein namesi
Recommended name:
Major allergen I polypeptide chain 2
Alternative name(s):
AG4
Allergen Cat-1
Allergen Fel d I-B
Short name:
Allergen FdI
Allergen: Fel d 1-B
Gene namesi
Name:CH2
OrganismiFelis catus (Cat) (Felis silvestris catus)
Taxonomic identifieri9685 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraFeliformiaFelidaeFelinaeFelis
ProteomesiUP000011712: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. Binds to IgE. Major allergen produced by the domestic cat. Implicated as an asthma-inducing agent in human. This protein is sticky and easily adheres to walls, carpet, clothing, furniture and bedding.

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei3278. Fel d 1.0101.
345. Fel d 1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 17172 PublicationsAdd
BLAST
Chaini18 – 10992Major allergen I polypeptide chain 2PRO_0000021248Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi24 – 24Interchain (with C-92 in chain 1)
Glycosylationi50 – 501N-linked (GlcNAc...)
Disulfide bondi65 – 65Interchain (with C-66 in chain 1)
Disulfide bondi90 – 90Interchain (with C-25 in chain 1)

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

The long form is preferentially expressed in the salivary gland, while the short form is preferentially expressed in the skin.

Interactioni

Subunit structurei

Heterotetramer composed of two non-covalently linked disulfide-linked heterodimer of chains 1 and 2.

Structurei

Secondary structure

1
109
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi19 – 235Combined sources
Helixi25 – 3511Combined sources
Helixi39 – 4810Combined sources
Helixi53 – 6816Combined sources
Helixi71 – 733Combined sources
Beta strandi74 – 763Combined sources
Helixi77 – 859Combined sources
Turni88 – 903Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PUOX-ray1.85A/B18-109[»]
1ZKRX-ray1.64A/B18-93[»]
2EJNX-ray1.64A/B18-93[»]
ProteinModelPortaliP30440.
SMRiP30440. Positions 22-91.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP30440.

Family & Domainsi

Sequence similaritiesi

Belongs to the secretoglobin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG063446.
InParanoidiP30440.

Family and domain databases

InterProiIPR015332. Allergen_Fel_d_1_chain2.
IPR016126. Secretoglobin.
[Graphical view]
PfamiPF09252. Feld-I_B. 1 hit.
[Graphical view]
SUPFAMiSSF48201. SSF48201. 1 hit.
PROSITEiPS51311. SCGB. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. Align

Note: Experimental confirmation may be lacking for some isoforms.

Isoform 1 (identifier: P30440-1) [UniParc]FASTAAdd to Basket

Also known as: CH2L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MRGALLVLAL LVTQALGVKM AETCPIFYDV FFAVANGNEL LLDLSLTKVN
60 70 80 90 100
ATEPERTAMK KIQDCYVENG LISRVLDGLV MTTISSSKDC MGEAVQNTVE

DLKLNTLGR
Length:109
Mass (Da):11,854
Last modified:April 1, 1993 - v1
Checksum:i857FB9CD76036CB9
GO
Isoform 2 (identifier: P30440-2) [UniParc]FASTAAdd to Basket

Also known as: CH2S

The sequence of this isoform differs from the canonical sequence as follows:
     82-89: TTISSSKD → IAINEY

Show »
Length:107
Mass (Da):11,738
Checksum:i3D8AD80A8251E0B2
GO
Isoform 3 (identifier: P30440-3) [UniParc]FASTAAdd to Basket

Also known as: CH2ST, Truncated

The sequence of this isoform differs from the canonical sequence as follows:
     82-109: TTISSSKDCMGEAVQNTVEDLKLNTLGR → PSTNIAWVKQFRTP

Show »
Length:95
Mass (Da):10,487
Checksum:i0E5448722934E6E2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti24 – 241C → F AA sequence (PubMed:1712068)Curated
Sequence conflicti32 – 321F → T AA sequence (PubMed:1712068)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti72 – 721I → L in CH2LV.
Natural varianti72 – 721I → V in CH2SV.
Natural varianti74 – 752RV → KF in CH2SV.
Natural varianti91 – 911M → T in CH2LV.
Natural varianti96 – 961Q → E in CH2SV.
Natural varianti105 – 1051N → K in CH2SV.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei82 – 10928TTISS…NTLGR → PSTNIAWVKQFRTP in isoform 3. CuratedVSP_004248Add
BLAST
Alternative sequencei82 – 898TTISSSKD → IAINEY in isoform 2. CuratedVSP_004249

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M77341 mRNA. Translation: AAC41616.1.
X62478 Genomic DNA. Translation: CAA44345.1.
PIRiB53283.
C56413.
JC1127.
RefSeqiNP_001041619.1. NM_001048154.1. [P30440-1]

Genome annotation databases

GeneIDi677879.
KEGGifca:677879.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M77341 mRNA. Translation: AAC41616.1 .
X62478 Genomic DNA. Translation: CAA44345.1 .
PIRi B53283.
C56413.
JC1127.
RefSeqi NP_001041619.1. NM_001048154.1. [P30440-1 ]

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1PUO X-ray 1.85 A/B 18-109 [» ]
1ZKR X-ray 1.64 A/B 18-93 [» ]
2EJN X-ray 1.64 A/B 18-93 [» ]
ProteinModelPortali P30440.
SMRi P30440. Positions 22-91.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

Allergomei 3278. Fel d 1.0101.
345. Fel d 1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 677879.
KEGGi fca:677879.

Organism-specific databases

CTDi 677879.

Phylogenomic databases

HOVERGENi HBG063446.
InParanoidi P30440.

Miscellaneous databases

EvolutionaryTracei P30440.

Family and domain databases

InterProi IPR015332. Allergen_Fel_d_1_chain2.
IPR016126. Secretoglobin.
[Graphical view ]
Pfami PF09252. Feld-I_B. 1 hit.
[Graphical view ]
SUPFAMi SSF48201. SSF48201. 1 hit.
PROSITEi PS51311. SCGB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Amino acid sequence of Fel dI, the major allergen of the domestic cat: protein sequence analysis and cDNA cloning."
    Morgenstern J.P., Griffith I.J., Brauer A.W., Rogers B.L., Bond J.F., Chapman M.D., Kuo M.-C.
    Proc. Natl. Acad. Sci. U.S.A. 88:9690-9694(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 18-100.
  2. "Expression and genomic structure of the genes encoding FdI, the major allergen from the domestic cat."
    Griffith I.J., Craig S., Pollock J., Yu X.-B., Morgenstern J.P., Rogers B.L.
    Gene 113:263-268(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE, ALTERNATIVE SPLICING, VARIANTS.
    Tissue: Liver.
  3. "Studies on the biochemical structure of the major cat allergen Felis domesticus I."
    Duffort O.A., Carreira J., Nitti G., Polo F., Lombardero M.
    Mol. Immunol. 28:301-309(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 18-37, CHARACTERIZATION.
  4. "Cat allergen 1: biochemical, antigenic, and allergenic properties."
    Leitermann K., Ohman J.L. Jr.
    J. Allergy Clin. Immunol. 74:147-153(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. "The crystal structure of the major cat allergen Fel d 1, a member of the secretoglobin family."
    Kaiser L., Gronlund H., Sandalova T., Ljunggren H.G., van Hage-Hamsten M., Achour A., Schneider G.
    J. Biol. Chem. 278:37730-37735(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) OF 18-109.

Entry informationi

Entry nameiFEL1B_FELCA
AccessioniPrimary (citable) accession number: P30440
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: November 26, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3