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P30440 (FEL1B_FELCA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Major allergen I polypeptide chain 2
Alternative name(s):
AG4
Allergen Cat-1
Allergen Fel d I-B
Short name=Allergen FdI
Allergen=Fel d 1-B
Gene names
Name:CH2
OrganismFelis catus (Cat) (Felis silvestris catus) [Reference proteome]
Taxonomic identifier9685 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraFeliformiaFelidaeFelinaeFelis

Protein attributes

Sequence length109 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Subunit structure

Heterotetramer composed of two non-covalently linked disulfide-linked heterodimer of chains 1 and 2.

Subcellular location

Secreted.

Tissue specificity

The long form is preferentially expressed in the salivary gland, while the short form is preferentially expressed in the skin.

Allergenic properties

Causes an allergic reaction in human. Binds to IgE. Major allergen produced by the domestic cat. Implicated as an asthma-inducing agent in human. This protein is sticky and easily adheres to walls, carpet, clothing, furniture and bedding.

Sequence similarities

Belongs to the secretoglobin family.

Ontologies

Keywords
   Cellular componentSecreted
   Coding sequence diversityAlternative splicing
Polymorphism
   DiseaseAllergen
   DomainSignal
   PTMDisulfide bond
Glycoprotein
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Cellular_componentextracellular space

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Note: Experimental confirmation may be lacking for some isoforms.
Isoform 1 (identifier: P30440-1)

Also known as: CH2L;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P30440-2)

Also known as: CH2S;

The sequence of this isoform differs from the canonical sequence as follows:
     82-89: TTISSSKD → IAINEY
Isoform 3 (identifier: P30440-3)

Also known as: CH2ST; Truncated;

The sequence of this isoform differs from the canonical sequence as follows:
     82-109: TTISSSKDCMGEAVQNTVEDLKLNTLGR → PSTNIAWVKQFRTP

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1717 Ref.1 Ref.3
Chain18 – 10992Major allergen I polypeptide chain 2
PRO_0000021248

Amino acid modifications

Glycosylation501N-linked (GlcNAc...)
Disulfide bond24Interchain (with C-92 in chain 1)
Disulfide bond65Interchain (with C-66 in chain 1)
Disulfide bond90Interchain (with C-25 in chain 1)

Natural variations

Alternative sequence82 – 10928TTISS…NTLGR → PSTNIAWVKQFRTP in isoform 3.
VSP_004248
Alternative sequence82 – 898TTISSSKD → IAINEY in isoform 2.
VSP_004249
Natural variant721I → L in CH2LV.
Natural variant721I → V in CH2SV.
Natural variant74 – 752RV → KF in CH2SV.
Natural variant911M → T in CH2LV.
Natural variant961Q → E in CH2SV.
Natural variant1051N → K in CH2SV.

Experimental info

Sequence conflict241C → F AA sequence Ref.3
Sequence conflict321F → T AA sequence Ref.3

Secondary structure

............... 109
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (CH2L) [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: 857FB9CD76036CB9

FASTA10911,854
        10         20         30         40         50         60 
MRGALLVLAL LVTQALGVKM AETCPIFYDV FFAVANGNEL LLDLSLTKVN ATEPERTAMK 

        70         80         90        100 
KIQDCYVENG LISRVLDGLV MTTISSSKDC MGEAVQNTVE DLKLNTLGR 

« Hide

Isoform 2 (CH2S) [UniParc].

Checksum: 3D8AD80A8251E0B2
Show »

FASTA10711,738
Isoform 3 (CH2ST) (Truncated) [UniParc].

Checksum: 0E5448722934E6E2
Show »

FASTA9510,487

References

[1]"Amino acid sequence of Fel dI, the major allergen of the domestic cat: protein sequence analysis and cDNA cloning."
Morgenstern J.P., Griffith I.J., Brauer A.W., Rogers B.L., Bond J.F., Chapman M.D., Kuo M.-C.
Proc. Natl. Acad. Sci. U.S.A. 88:9690-9694(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 18-100.
[2]"Expression and genomic structure of the genes encoding FdI, the major allergen from the domestic cat."
Griffith I.J., Craig S., Pollock J., Yu X.-B., Morgenstern J.P., Rogers B.L.
Gene 113:263-268(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE, ALTERNATIVE SPLICING, VARIANTS.
Tissue: Liver.
[3]"Studies on the biochemical structure of the major cat allergen Felis domesticus I."
Duffort O.A., Carreira J., Nitti G., Polo F., Lombardero M.
Mol. Immunol. 28:301-309(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 18-37, CHARACTERIZATION.
[4]"Cat allergen 1: biochemical, antigenic, and allergenic properties."
Leitermann K., Ohman J.L. Jr.
J. Allergy Clin. Immunol. 74:147-153(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[5]"The crystal structure of the major cat allergen Fel d 1, a member of the secretoglobin family."
Kaiser L., Gronlund H., Sandalova T., Ljunggren H.G., van Hage-Hamsten M., Achour A., Schneider G.
J. Biol. Chem. 278:37730-37735(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) OF 18-109.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M77341 mRNA. Translation: AAC41616.1.
X62478 Genomic DNA. Translation: CAA44345.1.
PIRB53283.
C56413.
JC1127.
RefSeqNP_001041619.1. NM_001048154.1. [P30440-1]

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1PUOX-ray1.85A/B18-109[»]
1ZKRX-ray1.64A/B18-93[»]
2EJNX-ray1.64A/B18-93[»]
ProteinModelPortalP30440.
SMRP30440. Positions 22-91.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

Allergome3278. Fel d 1.0101.
345. Fel d 1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID677879.
KEGGfca:677879.

Organism-specific databases

CTD677879.

Phylogenomic databases

HOVERGENHBG063446.

Family and domain databases

InterProIPR015332. Allergen_Fel_d_1_chain2.
IPR016126. Secretoglobin.
[Graphical view]
PfamPF09252. Feld-I_B. 1 hit.
[Graphical view]
SUPFAMSSF48201. SSF48201. 1 hit.
PROSITEPS51311. SCGB. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP30440.

Entry information

Entry nameFEL1B_FELCA
AccessionPrimary (citable) accession number: P30440
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: May 14, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Allergens

Nomenclature of allergens and list of entries