P30440 (FEL1B_FELCA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Major allergen I polypeptide chain 2

Alternative name(s):
AG4
Allergen Cat-1
Allergen Fel d I-B
Short name=Allergen FdI
Allergen=Fel d 1-B

Gene names

Name:

CH2

Organism

Felis catus (Cat) (Felis silvestris catus) [Complete proteome]

Taxonomic identifier

9685 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Carnivora › Feliformia › Felidae › Felinae › Felis

Protein attributes

Sequence length	109 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Heterotetramer composed of two non-covalently linked disulfide-linked heterodimer of chains 1 and 2.
Subcellular location	Secreted.
Tissue specificity	The long form is preferentially expressed in the salivary gland, while the short form is preferentially expressed in the skin.
Allergenic properties	Causes an allergic reaction in human. Binds to IgE. Major allergen produced by the domestic cat. Implicated as an asthma-inducing agent in human. This protein is sticky and easily adheres to walls, carpet, clothing, furniture and bedding.
Sequence similarities	Belongs to the secretoglobin family.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing Polymorphism
Disease	Allergen
Domain	Signal
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Cellular_component	extracellular space Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Note: Experimental confirmation may be lacking for some isoforms.

Isoform 1 (identifier: P30440-1)

Also known as: CH2L;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P30440-2)

Also known as: CH2S;

The sequence of this isoform differs from the canonical sequence as follows:
     82-89: TTISSSKD → IAINEY

Isoform 3 (identifier: P30440-3)

Also known as: CH2ST; Truncated;

The sequence of this isoform differs from the canonical sequence as follows:
     82-109: TTISSSKDCMGEAVQNTVEDLKLNTLGR → PSTNIAWVKQFRTP

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 17

17

Ref.1 Ref.3

Chain

18 – 109

92

Major allergen I polypeptide chain 2

PRO_0000021248

Amino acid modifications

Glycosylation

50

1

N-linked (GlcNAc...)

Disulfide bond

24

Interchain (with C-92 in chain 1)

Disulfide bond

65

Interchain (with C-66 in chain 1)

Disulfide bond

90

Interchain (with C-25 in chain 1)

Natural variations

Alternative sequence

82 – 109

28

TTISS…NTLGR → PSTNIAWVKQFRTP in isoform 3.

VSP_004248

Alternative sequence

82 – 89

8

TTISSSKD → IAINEY in isoform 2.

VSP_004249

Natural variant

72

1

I → L in CH2LV.

Natural variant

72

1

I → V in CH2SV.

Natural variant

74 – 75

2

RV → KF in CH2SV.

Natural variant

91

1

M → T in CH2LV.

Natural variant

96

1

Q → E in CH2SV.

Natural variant

105

1

N → K in CH2SV.

Experimental info

Sequence conflict

24

1

C → F AA sequence Ref.3

Sequence conflict

32

1

F → T AA sequence Ref.3

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

109

Helix Strand Turn

Details...

Beta strand

19 – 23

5

Helix

25 – 35

11

Helix

39 – 48

10

Helix

53 – 68

16

Helix

71 – 73

3

Beta strand

74 – 76

3

Helix

77 – 85

9

Turn

88 – 90

3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (CH2L) [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 857FB9CD76036CB9	FASTA	109	11,854
	10 20 30 40 50 60 MRGALLVLAL LVTQALGVKM AETCPIFYDV FFAVANGNEL LLDLSLTKVN ATEPERTAMK 70 80 90 100 KIQDCYVENG LISRVLDGLV MTTISSSKDC MGEAVQNTVE DLKLNTLGR
	Isoform 2 (CH2S) [UniParc]. Checksum: 3D8AD80A8251E0B2	FASTA	107	11,738
	10 20 30 40 50 60 MRGALLVLAL LVTQALGVKM AETCPIFYDV FFAVANGNEL LLDLSLTKVN ATEPERTAMK 70 80 90 100 KIQDCYVENG LISRVLDGLV MIAINEYCMG EAVQNTVEDL KLNTLGR
	Isoform 3 (CH2ST) (Truncated) [UniParc]. Checksum: 0E5448722934E6E2	FASTA	95	10,487
	10 20 30 40 50 60 MRGALLVLAL LVTQALGVKM AETCPIFYDV FFAVANGNEL LLDLSLTKVN ATEPERTAMK 70 80 90 KIQDCYVENG LISRVLDGLV MPSTNIAWVK QFRTP

References

[1]	"Amino acid sequence of Fel dI, the major allergen of the domestic cat: protein sequence analysis and cDNA cloning." Morgenstern J.P., Griffith I.J., Brauer A.W., Rogers B.L., Bond J.F., Chapman M.D., Kuo M.-C. Proc. Natl. Acad. Sci. U.S.A. 88:9690-9694(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 18-100.
[2]	"Expression and genomic structure of the genes encoding FdI, the major allergen from the domestic cat." Griffith I.J., Craig S., Pollock J., Yu X.-B., Morgenstern J.P., Rogers B.L. Gene 113:263-268(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE, ALTERNATIVE SPLICING, VARIANTS. Tissue: Liver.
[3]	"Studies on the biochemical structure of the major cat allergen Felis domesticus I." Duffort O.A., Carreira J., Nitti G., Polo F., Lombardero M. Mol. Immunol. 28:301-309(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 18-37, CHARACTERIZATION.
[4]	"Cat allergen 1: biochemical, antigenic, and allergenic properties." Leitermann K., Ohman J.L. Jr. J. Allergy Clin. Immunol. 74:147-153(1984) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[5]	"The crystal structure of the major cat allergen Fel d 1, a member of the secretoglobin family." Kaiser L., Gronlund H., Sandalova T., Ljunggren H.G., van Hage-Hamsten M., Achour A., Schneider G. J. Biol. Chem. 278:37730-37735(2003) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) OF 18-109.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M77341 mRNA. Translation: AAC41616.1.
X62478 Genomic DNA. Translation: CAA44345.1.

PIR

B53283.
C56413.
JC1127.

RefSeq

NP_001041619.1. NM_001048154.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1PUO	X-ray	1.85	A/B	18-109	[»]
1ZKR	X-ray	1.64	A/B	18-93	[»]
2EJN	X-ray	1.64	A/B	18-93	[»]

ProteinModelPortal

P30440.

SMR

P30440. Positions 22-91.

ModBase

Search...

Protein family/group databases

Allergome

3278. Fel d 1.0101.
345. Fel d 1.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

677879.

KEGG

fca:677879.

Organism-specific databases

CTD

677879.

Phylogenomic databases

HOVERGEN

HBG063446.

Family and domain databases

InterPro

IPR015332. Allergen_Fel_d_1_chain2.
IPR016126. Secretoglobin.
[Graphical view]

Pfam

PF09252. Feld-I_B. 1 hit.
[Graphical view]

SUPFAM

SSF48201. Secretoglobin. 1 hit.

PROSITE

PS51311. SCGB. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P30440.

Entry information

Entry name

FEL1B_FELCA

Accession

Primary (citable) accession number: P30440

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	May 1, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program