P30438 (FEL1A_FELCA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Major allergen I polypeptide chain 1

Alternative name(s):
AG4
Allergen Cat-1
Allergen Fel d I-A
Short name=Allergen FdI
Short name=Allergen Fel dI
Allergen=Fel d 1-A

Gene names

Name:

CH1

Organism

Felis catus (Cat) (Felis silvestris catus) [Complete proteome]

Taxonomic identifier

9685 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Carnivora › Feliformia › Felidae › Felinae › Felis

Protein attributes

Sequence length	92 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Heterotetramer composed of two non-covalently linked disulfide-linked heterodimer of chains 1 and 2.
Subcellular location	Secreted.
Tissue specificity	Saliva and sebaceous glands.
Allergenic properties	Causes an allergic reaction in human. Binds to IgE. Major allergen produced by the domestic cat. Implicated as an asthma-inducing agent in human. This protein is sticky and easily adheres to walls, carpet, clothing, furniture and bedding.
Sequence similarities	Belongs to the secretoglobin family.
Sequence caution	The sequence CAA44343.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA44344.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing Polymorphism
Disease	Allergen
Domain	Signal
PTM	Disulfide bond
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P30438-1)

Also known as: Leader A; Major;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P30438-2)

Also known as: Leader B; Minor;

The sequence of this isoform differs from the canonical sequence as follows:
     1-21: MKGACVLVLLWAALLLISGGN → MLDAALPPCPTVAATAD

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 22

22

Ref.1 Ref.3

Chain

23 – 92

70

Major allergen I polypeptide chain 1

PRO_0000036370

Amino acid modifications

Disulfide bond

25

Interchain (with C-90 in chain 2)

Disulfide bond

66

Interchain (with C-65 in chain 2)

Disulfide bond

92

Interchain (with C-24 in chain 2)

Natural variations

Alternative sequence

1 – 21

21

MKGAC…ISGGN → MLDAALPPCPTVAATAD in isoform 2.

VSP_015665

Natural variant

51

1

K → N.

Experimental info

Sequence conflict

5

1

C → R in AAC37318. Ref.1

Sequence conflict

18

1

S → W in AAC37318. Ref.1

Sequence conflict

82

1

V → L in AAC41617. Ref.1

Sequence conflict

82

1

V → L in AAC37318. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

92

Helix Strand Turn

Details...

Helix

26 – 37

12

Helix

40 – 50

11

Helix

54 – 70

17

Helix

73 – 87

15

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Leader A) (Major) [UniParc]. Last modified November 1, 1995. Version 2. Checksum: 083145F55EE8AAB3	FASTA	92	10,086
	10 20 30 40 50 60 MKGACVLVLL WAALLLISGG NCEICPAVKR DVDLFLTGTP DEYVEQVAQY KALPVVLENA 70 80 90 RILKNCVDAK MTEEDKENAL SVLDKIYTSP LC
	Isoform 2 (Leader B) (Minor) [UniParc]. Checksum: B378D6D99B547B57	FASTA	88	9,600
	10 20 30 40 50 60 MLDAALPPCP TVAATADCEI CPAVKRDVDL FLTGTPDEYV EQVAQYKALP VVLENARILK 70 80 NCVDAKMTEE DKENALSVLD KIYTSPLC

References

[1]	"Amino acid sequence of Fel dI, the major allergen of the domestic cat: protein sequence analysis and cDNA cloning." Morgenstern J.P., Griffith I.J., Brauer A.W., Rogers B.L., Bond J.F., Chapman M.D., Kuo M.-C. Proc. Natl. Acad. Sci. U.S.A. 88:9690-9694(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 23-92 (ISOFORMS 1 AND 2). Tissue: Salivary gland.
[2]	"Expression and genomic structure of the genes encoding FdI, the major allergen from the domestic cat." Griffith I.J., Craig S., Pollock J., Yu X.-B., Morgenstern J.P., Rogers B.L. Gene 113:263-268(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1 AND 2). Tissue: Liver.
[3]	"Studies on the biochemical structure of the major cat allergen Felis domesticus I." Duffort O.A., Carreira J., Nitti G., Polo F., Lombardero M. Mol. Immunol. 28:301-309(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 23-62, CHARACTERIZATION.
[4]	"Cat allergen 1: biochemical, antigenic, and allergenic properties." Leitermann K., Ohman J.L. Jr. J. Allergy Clin. Immunol. 74:147-153(1984) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[5]	"The crystal structure of the major cat allergen Fel d 1, a member of the secretoglobin family." Kaiser L., Gronlund H., Sandalova T., Ljunggren H.G., van Hage-Hamsten M., Achour A., Schneider G. J. Biol. Chem. 278:37730-37735(2003) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M74952 mRNA. Translation: AAC37318.1.
M74953 mRNA. Translation: AAC41617.1.
X62477 Genomic DNA. Translation: CAA44344.1. Different initiation.
X62477 Genomic DNA. Translation: CAA44343.1. Different initiation.

PIR

A56413.
JC1136.

RefSeq

NP_001041618.1. NM_001048153.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1PUO	X-ray	1.85	A/B	23-92	[»]
1ZKR	X-ray	1.64	A/B	23-92	[»]
2EJN	X-ray	1.64	A/B	23-92	[»]

ProteinModelPortal

P30438.

SMR

P30438. Positions 18-92.

ModBase

Search...

Protein family/group databases

Allergome

3278. Fel d 1.0101.
345. Fel d 1.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

677877.

KEGG

fca:677877.

Phylogenomic databases

HOVERGEN

HBG051604.

Family and domain databases

InterPro

IPR006178. Allergen_Fel_d_I_chain1.
IPR016126. Secretoglobin.
IPR006039. Uteroglobin-like_fam.
IPR006038. Uteroglobin-like_superfam.
[Graphical view]

PANTHER

PTHR21226. PTHR21226. 1 hit.

Pfam

PF01099. Uteroglobin. 1 hit.
[Graphical view]

PRINTS

PR00827. FELALLERGEN.

SMART

SM00096. UTG. 1 hit.
[Graphical view]

SUPFAM

SSF48201. Secretoglobin. 1 hit.

PROSITE

PS51311. SCGB. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P30438.

Entry information

Entry name

FEL1A_FELCA

Accession

Primary (citable) accession number: P30438
Secondary accession number(s): P30439

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 90 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program