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P30437 (CP17A_ONCMY) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Steroid 17-alpha-hydroxylase/17,20 lyase

EC=1.14.99.9
EC=4.1.2.30
Alternative name(s):
17-alpha-hydroxyprogesterone aldolase
CYPXVII
Cytochrome P450 17A1
Cytochrome P450-C17
Short name=Cytochrome P450c17
Gene names
Name:cyp17a1
Synonyms:cyp17
OrganismOncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)
Taxonomic identifier8022 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiProtacanthopterygiiSalmoniformesSalmonidaeSalmoninaeOncorhynchus

Protein attributes

Sequence length514 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction.

Catalytic activity

A C(21)-steroid + (reduced NADPH--hemoprotein reductase) + O2 = a 17-alpha-hydroxy-C(21)-steroid + (oxidized NADPH--hemoprotein reductase) + H2O.

17-alpha-hydroxyprogesterone = androst-4-ene-3,17-dione + acetaldehyde.

Cofactor

Heme group By similarity.

Enzyme regulation

Regulated predominantly by intracellular cAMP levels.

Pathway

Lipid metabolism; steroid biosynthesis.

Subcellular location

Membrane Potential.

Sequence similarities

Belongs to the cytochrome P450 family.

Ontologies

Keywords
   Biological processSteroidogenesis
   Cellular componentMembrane
   LigandHeme
Iron
Metal-binding
   Molecular functionLyase
Monooxygenase
Oxidoreductase
Gene Ontology (GO)
   Biological_processsteroid biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentmembrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function17-alpha-hydroxyprogesterone aldolase activity

Inferred from electronic annotation. Source: UniProtKB-EC

heme binding

Inferred from electronic annotation. Source: InterPro

iron ion binding

Inferred from electronic annotation. Source: InterPro

steroid 17-alpha-monooxygenase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 514514Steroid 17-alpha-hydroxylase/17,20 lyase
PRO_0000051944

Sites

Metal binding4491Iron (heme axial ligand) By similarity

Sequences

Sequence LengthMass (Da)Tools
P30437 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: 9AC19961F921DCCB

FASTA51457,408
        10         20         30         40         50         60 
MAWFLCMCVF SVVGLGLLLL QVKLRRSLET RGGPPSLPVF PLIGSLLSLR SNQAPHVLFQ 

        70         80         90        100        110        120 
KLQQKYGHTY SLMMGPHTVI LVNHHQHAKE VLLKKGKIFA GRPRTVTTDL LTRDGKDIAF 

       130        140        150        160        170        180 
ADYGATWRFH RKTVHGALCM FGEGSASIEK IICREALSLC DTLRESGSAS LDLSPELTRA 

       190        200        210        220        230        240 
VTNVVCSLCF SSSYCRGDPE FEAMLQFSQG IVDTVAKDSL VDIFPWLQVF PNADLRLLKQ 

       250        260        270        280        290        300 
CVSIRDKLLQ KKYEEHKSDY SDHEQRDLLD ALLRAKRSAE NNNTAEITME TVGLSEDHLL 

       310        320        330        340        350        360 
MTVGDIFGAG VETTSTVLKW AIAYLIHHPQ VQQRIQEELD SVVGGDRTPQ LSDRGSLPYL 

       370        380        390        400        410        420 
EATIREVLRI RPVAPLLIPH VAQTDTSIGK FTVRKGARII INLWSLHHDE KEWKNPEMFD 

       430        440        450        460        470        480 
PGRFLNEEGT GLCIPSPSYL PFGAGVRVCL GEALAKMEIF LFLSWILQRL TMTVSPGQPL 

       490        500        510 
PSLEGKFGVV LQPVKYKVNA TPRAGWEKSH LQTS 

« Hide

References

[1]"Rainbow trout cytochrome P-450c17 (17 alpha-hydroxylase/17,20-lyase). cDNA cloning, enzymatic properties and temporal pattern of ovarian P-450c17 mRNA expression during oogenesis."
Sakai N., Tanaka M., Adachi S., Miller W.L., Nagahama Y.
FEBS Lett. 301:60-64(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X65800 mRNA. Translation: CAA46675.1.
PIRS21125.
RefSeqNP_001118219.1. NM_001124747.1.
UniGeneOmy.8196.

3D structure databases

ProteinModelPortalP30437.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100137017.

Organism-specific databases

CTD1586.

Phylogenomic databases

HOVERGENHBG106944.

Enzyme and pathway databases

UniPathwayUPA00062.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCP17A_ONCMY
AccessionPrimary (citable) accession number: P30437
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: June 11, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways