Reviewed, UniProtKB/Swiss-Prot P30437 (CP17A_ONCMY)

Names and origin

Protein names

Recommended name:
    Steroid 17-alpha-hydroxylase/17,20 lyase
    EC=1.14.99.9
Alternative name(s):
    Cytochrome P450 17A1
    CYPXVII
    P450-C17
      Short name=P450c17

Gene names

Name:	cyp17a1
Synonyms:	cyp17

Organism

Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)

Taxonomic identifier

8022 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Euteleostei › Protacanthopterygii › Salmoniformes › Salmonidae › Salmoninae › Oncorhynchus

Protein attributes

Sequence length	514 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction.
Catalytic activity	A steroid + AH2 + O2 = a 17-alpha-hydroxysteroid + A + H2O.
Cofactor	Heme group By similarity.
Enzyme regulation	Regulated predominantly by intracellular cAMP levels.
Pathway	Lipid metabolism; steroid biosynthesis.
Subcellular location	Membrane Potential. Membrane; Single-pass membrane protein.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Steroidogenesis
Cellular component	Membrane
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	C21-steroid hormone biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro steroid 17-alpha-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 514	514	Steroid 17-alpha-hydroxylase/17,20 lyase		PRO_0000051944
Sites
	Metal binding	449	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P30437-1 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 9AC19961F921DCCB	FASTA	514	57,408
	10 20 30 40 50 60 MAWFLCMCVF SVVGLGLLLL QVKLRRSLET RGGPPSLPVF PLIGSLLSLR SNQAPHVLFQ 70 80 90 100 110 120 KLQQKYGHTY SLMMGPHTVI LVNHHQHAKE VLLKKGKIFA GRPRTVTTDL LTRDGKDIAF 130 140 150 160 170 180 ADYGATWRFH RKTVHGALCM FGEGSASIEK IICREALSLC DTLRESGSAS LDLSPELTRA 190 200 210 220 230 240 VTNVVCSLCF SSSYCRGDPE FEAMLQFSQG IVDTVAKDSL VDIFPWLQVF PNADLRLLKQ 250 260 270 280 290 300 CVSIRDKLLQ KKYEEHKSDY SDHEQRDLLD ALLRAKRSAE NNNTAEITME TVGLSEDHLL 310 320 330 340 350 360 MTVGDIFGAG VETTSTVLKW AIAYLIHHPQ VQQRIQEELD SVVGGDRTPQ LSDRGSLPYL 370 380 390 400 410 420 EATIREVLRI RPVAPLLIPH VAQTDTSIGK FTVRKGARII INLWSLHHDE KEWKNPEMFD 430 440 450 460 470 480 PGRFLNEEGT GLCIPSPSYL PFGAGVRVCL GEALAKMEIF LFLSWILQRL TMTVSPGQPL 490 500 510 PSLEGKFGVV LQPVKYKVNA TPRAGWEKSH LQTS

References

[1]

"Rainbow trout cytochrome P-450c17 (17 alpha-hydroxylase/17,20-lyase). cDNA cloning, enzymatic properties and temporal pattern of ovarian P-450c17 mRNA expression during oogenesis." Sakai N., Tanaka M., Adachi S., Miller W.L., Nagahama Y. FEBS Lett. 301:60-64(1992) [PubMed: 1451787] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X65800 mRNA. Translation: CAA46675.1.
PIR	S21125.
RefSeq	NP_001118219.1.
UniGene	Omy.8196
3D structure databases
HSSP	HSSP built from PDB template 1DT6 based on UniProtKB P00179.
ModBase	Search...
Genome annotation databases
GeneID	100137017.
Phylogenomic databases
HOVERGEN	P30437.
Enzyme and pathway databases
BRENDA	1.14.99.9. 3435.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP17A_ONCMY

Accession

Primary (citable) accession number: P30437

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	June 16, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)