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P30437

- CP17A_ONCMY

UniProt

P30437 - CP17A_ONCMY

Protein

Steroid 17-alpha-hydroxylase/17,20 lyase

Gene

cyp17a1

Organism
Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 1 (01 Apr 1993)
      Previous versions | rss
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    Functioni

    Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction.

    Catalytic activityi

    A C(21)-steroid + (reduced NADPH--hemoprotein reductase) + O2 = a 17-alpha-hydroxy-C(21)-steroid + (oxidized NADPH--hemoprotein reductase) + H2O.
    17-alpha-hydroxyprogesterone = androst-4-ene-3,17-dione + acetaldehyde.

    Cofactori

    Heme group.By similarity

    Enzyme regulationi

    Regulated predominantly by intracellular cAMP levels.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi449 – 4491Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    1. 17-alpha-hydroxyprogesterone aldolase activity Source: UniProtKB-EC
    2. heme binding Source: InterPro
    3. iron ion binding Source: InterPro
    4. steroid 17-alpha-monooxygenase activity Source: UniProtKB-EC

    GO - Biological processi

    1. steroid biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase, Monooxygenase, Oxidoreductase

    Keywords - Biological processi

    Steroidogenesis

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00062.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Steroid 17-alpha-hydroxylase/17,20 lyase (EC:1.14.99.9, EC:4.1.2.30)
    Alternative name(s):
    17-alpha-hydroxyprogesterone aldolase
    CYPXVII
    Cytochrome P450 17A1
    Cytochrome P450-C17
    Short name:
    Cytochrome P450c17
    Gene namesi
    Name:cyp17a1
    Synonyms:cyp17
    OrganismiOncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)
    Taxonomic identifieri8022 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiProtacanthopterygiiSalmoniformesSalmonidaeSalmoninaeOncorhynchus

    Subcellular locationi

    Membrane Curated

    GO - Cellular componenti

    1. membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 514514Steroid 17-alpha-hydroxylase/17,20 lyasePRO_0000051944Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP30437.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    HOVERGENiHBG106944.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P30437-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAWFLCMCVF SVVGLGLLLL QVKLRRSLET RGGPPSLPVF PLIGSLLSLR    50
    SNQAPHVLFQ KLQQKYGHTY SLMMGPHTVI LVNHHQHAKE VLLKKGKIFA 100
    GRPRTVTTDL LTRDGKDIAF ADYGATWRFH RKTVHGALCM FGEGSASIEK 150
    IICREALSLC DTLRESGSAS LDLSPELTRA VTNVVCSLCF SSSYCRGDPE 200
    FEAMLQFSQG IVDTVAKDSL VDIFPWLQVF PNADLRLLKQ CVSIRDKLLQ 250
    KKYEEHKSDY SDHEQRDLLD ALLRAKRSAE NNNTAEITME TVGLSEDHLL 300
    MTVGDIFGAG VETTSTVLKW AIAYLIHHPQ VQQRIQEELD SVVGGDRTPQ 350
    LSDRGSLPYL EATIREVLRI RPVAPLLIPH VAQTDTSIGK FTVRKGARII 400
    INLWSLHHDE KEWKNPEMFD PGRFLNEEGT GLCIPSPSYL PFGAGVRVCL 450
    GEALAKMEIF LFLSWILQRL TMTVSPGQPL PSLEGKFGVV LQPVKYKVNA 500
    TPRAGWEKSH LQTS 514
    Length:514
    Mass (Da):57,408
    Last modified:April 1, 1993 - v1
    Checksum:i9AC19961F921DCCB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X65800 mRNA. Translation: CAA46675.1.
    PIRiS21125.
    RefSeqiNP_001118219.1. NM_001124747.1.
    UniGeneiOmy.8196.

    Genome annotation databases

    GeneIDi100137017.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X65800 mRNA. Translation: CAA46675.1 .
    PIRi S21125.
    RefSeqi NP_001118219.1. NM_001124747.1.
    UniGenei Omy.8196.

    3D structure databases

    ProteinModelPortali P30437.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100137017.

    Organism-specific databases

    CTDi 1586.

    Phylogenomic databases

    HOVERGENi HBG106944.

    Enzyme and pathway databases

    UniPathwayi UPA00062 .

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Rainbow trout cytochrome P-450c17 (17 alpha-hydroxylase/17,20-lyase). cDNA cloning, enzymatic properties and temporal pattern of ovarian P-450c17 mRNA expression during oogenesis."
      Sakai N., Tanaka M., Adachi S., Miller W.L., Nagahama Y.
      FEBS Lett. 301:60-64(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiCP17A_ONCMY
    AccessioniPrimary (citable) accession number: P30437
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: April 1, 1993
    Last modified: October 1, 2014
    This is version 89 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3