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Protein

Steroid 17-alpha-hydroxylase/17,20 lyase

Gene

cyp17a1

Organism
Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Conversion of pregnenolone and progesterone to their 17-alpha-hydroxylated products and subsequently to dehydroepiandrosterone (DHEA) and androstenedione. Catalyzes both the 17-alpha-hydroxylation and the 17,20-lyase reaction.

Catalytic activityi

A C(21)-steroid + (reduced NADPH--hemoprotein reductase) + O2 = a 17-alpha-hydroxy-C(21)-steroid + (oxidized NADPH--hemoprotein reductase) + H2O.
17-alpha-hydroxyprogesterone = androst-4-ene-3,17-dione + acetaldehyde.

Cofactori

hemeBy similarity

Enzyme regulationi

Regulated predominantly by intracellular cAMP levels.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi449 – 4491Iron (heme axial ligand)By similarity

GO - Molecular functioni

  • 17-alpha-hydroxyprogesterone aldolase activity Source: UniProtKB-EC
  • heme binding Source: InterPro
  • iron ion binding Source: InterPro
  • steroid 17-alpha-monooxygenase activity Source: AgBase

GO - Biological processi

  • progesterone metabolic process Source: AgBase
  • steroid biosynthetic process Source: AgBase
Complete GO annotation...

Keywords - Molecular functioni

Lyase, Monooxygenase, Oxidoreductase

Keywords - Biological processi

Steroidogenesis

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00062.

Names & Taxonomyi

Protein namesi
Recommended name:
Steroid 17-alpha-hydroxylase/17,20 lyase (EC:1.14.99.9, EC:4.1.2.30)
Alternative name(s):
17-alpha-hydroxyprogesterone aldolase
CYPXVII
Cytochrome P450 17A1
Cytochrome P450-C17
Short name:
Cytochrome P450c17
Gene namesi
Name:cyp17a1
Synonyms:cyp17
OrganismiOncorhynchus mykiss (Rainbow trout) (Salmo gairdneri)
Taxonomic identifieri8022 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiProtacanthopterygiiSalmoniformesSalmonidaeSalmoninaeOncorhynchus

Subcellular locationi

GO - Cellular componenti

  • cell Source: AgBase
  • membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 514514Steroid 17-alpha-hydroxylase/17,20 lyasePRO_0000051944Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP30437.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

HOVERGENiHBG106944.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P30437-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAWFLCMCVF SVVGLGLLLL QVKLRRSLET RGGPPSLPVF PLIGSLLSLR
60 70 80 90 100
SNQAPHVLFQ KLQQKYGHTY SLMMGPHTVI LVNHHQHAKE VLLKKGKIFA
110 120 130 140 150
GRPRTVTTDL LTRDGKDIAF ADYGATWRFH RKTVHGALCM FGEGSASIEK
160 170 180 190 200
IICREALSLC DTLRESGSAS LDLSPELTRA VTNVVCSLCF SSSYCRGDPE
210 220 230 240 250
FEAMLQFSQG IVDTVAKDSL VDIFPWLQVF PNADLRLLKQ CVSIRDKLLQ
260 270 280 290 300
KKYEEHKSDY SDHEQRDLLD ALLRAKRSAE NNNTAEITME TVGLSEDHLL
310 320 330 340 350
MTVGDIFGAG VETTSTVLKW AIAYLIHHPQ VQQRIQEELD SVVGGDRTPQ
360 370 380 390 400
LSDRGSLPYL EATIREVLRI RPVAPLLIPH VAQTDTSIGK FTVRKGARII
410 420 430 440 450
INLWSLHHDE KEWKNPEMFD PGRFLNEEGT GLCIPSPSYL PFGAGVRVCL
460 470 480 490 500
GEALAKMEIF LFLSWILQRL TMTVSPGQPL PSLEGKFGVV LQPVKYKVNA
510
TPRAGWEKSH LQTS
Length:514
Mass (Da):57,408
Last modified:April 1, 1993 - v1
Checksum:i9AC19961F921DCCB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65800 mRNA. Translation: CAA46675.1.
PIRiS21125.
RefSeqiNP_001118219.1. NM_001124747.1.
UniGeneiOmy.8196.

Genome annotation databases

GeneIDi100137017.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65800 mRNA. Translation: CAA46675.1.
PIRiS21125.
RefSeqiNP_001118219.1. NM_001124747.1.
UniGeneiOmy.8196.

3D structure databases

ProteinModelPortaliP30437.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100137017.

Organism-specific databases

CTDi1586.

Phylogenomic databases

HOVERGENiHBG106944.

Enzyme and pathway databases

UniPathwayiUPA00062.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Rainbow trout cytochrome P-450c17 (17 alpha-hydroxylase/17,20-lyase). cDNA cloning, enzymatic properties and temporal pattern of ovarian P-450c17 mRNA expression during oogenesis."
    Sakai N., Tanaka M., Adachi S., Miller W.L., Nagahama Y.
    FEBS Lett. 301:60-64(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiCP17A_ONCMY
AccessioniPrimary (citable) accession number: P30437
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: May 27, 2015
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.