Reviewed, UniProtKB/Swiss-Prot P30430 (FUR1C_DROME)

Names and origin

Protein names

Recommended name:
    Furin-like protease 1, isoform 1-CRR
      Short name=Furin-1
    EC=3.4.21.75
Alternative name(s):
    Kex2-like endoprotease 1
    dKLIP-1

Gene names

Name:	Fur1
ORF Names:	CG10772

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1101 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Furin is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif By similarity.
Catalytic activity	Release of mature proteins from their proproteins by cleavage of -Arg-Xaa-Yaa-Arg-|-Zaa- bonds, where Xaa can be any amino acid and Yaa is Arg or Lys. Releases albumin, complement component C3 and vWF from their respective precursors.
Subcellular location	Golgi apparatus membrane; Multi-pass membrane protein. Ref.2
Tissue specificity	In adults, isoform 1-CRR is expressed in CNS, fat body, and female reproductive tissues, and in embryos, in anal pads, hindgut, developing antennomaxillary complex, oenocytes, clipeolabrum, pharynx, trachea, CNS and developing posterior spiracles. Ref.2
Developmental stage	Isoform 1-CRR is expressed in embryos, larvae, pupae and adults. Ref.2
Sequence similarities	Belongs to the peptidase S8 family. Furin subfamily.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal Transmembrane
Molecular function	Hydrolase Protease Serine protease
PTM	Cleavage on pair of basic residues Disulfide bond Glycoprotein Zymogen
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Ref.1 Inferred from direct assay. Source: FlyBase
Molecular function	serine-type endopeptidase activity Ref.1 Inferred from direct assay. Source: FlyBase
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1-CRR (identifier: P30430-1)

Also known as: A;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 1 (identifier: P26016-2)

Also known as: F;

The sequence of this isoform can be found in the external entry P26016-2.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Isoform 1-X (identifier: P26016-1)

Also known as: E;

The sequence of this isoform can be found in the external entry P26016-1.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Isoform 2 (identifier: P26016-3)

Also known as: C; D; F;

The sequence of this isoform can be found in the external entry P26016-3.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – ?		Potential
	Propeptide	? – 309		Potential		PRO_0000027022
	Chain	310 – 1101	792	Furin-like protease 1, isoform 1-CRR		PRO_0000027023
Regions
	Transmembrane	119 – 139	21	Potential
	Transmembrane	1014 – 1034	21	Potential
	Region	310 – 655	346	Catalytic
	Compositional bias	847 – 982	136	Cys-rich
Sites
	Active site	372	1	Charge relay system By similarity
	Active site	413	1	Charge relay system By similarity
	Active site	587	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	15	1	N-linked (GlcNAc...) Potential
	Glycosylation	18	1	N-linked (GlcNAc...) Potential
	Glycosylation	28	1	N-linked (GlcNAc...) Potential
	Glycosylation	108	1	N-linked (GlcNAc...) Potential
	Glycosylation	333	1	N-linked (GlcNAc...) Potential
	Glycosylation	426	1	N-linked (GlcNAc...) Potential
	Glycosylation	606	1	N-linked (GlcNAc...) Potential
	Glycosylation	727	1	N-linked (GlcNAc...) Potential
	Glycosylation	859	1	N-linked (GlcNAc...) Potential
	Glycosylation	978	1	N-linked (GlcNAc...) Potential
	Disulfide bond	430 ↔ 579		By similarity
	Disulfide bond	522 ↔ 552		By similarity
	Disulfide bond	669 ↔ 695		By similarity
Experimental info
	Sequence conflict	1014	1	T → I in AAA28467. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1-CRR (A) [UniParc]. Last modified June 1, 2001. Version 2. Checksum: 90DC38E2CACB71A0	FASTA	1,101	120,993
	10 20 30 40 50 60 MKNDVVRWSR QPTSNTTNSS SSSRSDSNST HKHRSKSNKL NARQLGSNAA RSCQQRSSVA 70 80 90 100 110 120 TTLEDEQQTI IECDIGNFNF DCNLFKTSFL TQHKQKRSGK SSSKSKSNRS RPLAKTKAVF 130 140 150 160 170 180 LLALQFSAVV FLCNINVGFV AGSVATAASS AGGSSPAAPS SAPSSPPTVA VPPPPPPSSA 190 200 210 220 230 240 LKVDPNGQSP VLPPYVLDYE TGGKAKLTPN NGKFGQSGSS GSNNNHIVGH YTHTWAVHIP 250 260 270 280 290 300 NGDNGMADAV AKDHGFVNLG KIFDDHYHFA HHKVSKRSLS PATHHQTRLD DDDRVHWAKQ 310 320 330 340 350 360 QRAKSRSKRD FIRMRPSRTS SRAMSMVDAM SFNDSKWPQM WYLNRGGGLD MNVIPAWKMG 370 380 390 400 410 420 ITGKGVVVTI LDDGLESDHP DIQDNYDPKA SYDVNSHDDD PMPHYDMTDS NRHGTRCAGE 430 440 450 460 470 480 VAATANNSFC AVGIAYGASV GGVRMLDGDV TDAVEARSLS LNPQHIDIYS ASWGPDDDGK 490 500 510 520 530 540 TVDGPGELAS RAFIEGTTKG RGGKGSIFIW ASGNGGREQD NCNCDGYTNS IWTLSISSAT 550 560 570 580 590 600 EEGHVPWYSE KCSSTLATTY SSGGQGEKQV VTTDLHHSCT VSHTGTSASA PLAAGIAALV 610 620 630 640 650 660 LQSNQNLTWR DLQHIVVRTA KPANLKDPSW SRNGVGRRVS HSFGYGLMDA AEMVRVARNW 670 680 690 700 710 720 KAVPEQQRCE INAPHVDKVI PPRTHITLQL TVNHCRSVNY LEHVQAKITL TSQRRGDIQL 730 740 750 760 770 780 FLRSPANTSV TLLTPRIHDN SRSGFNQWPF MSVHTWGESP QGNWQLEIHN EGRYMAQITQ 790 800 810 820 830 840 WDMIFYGTET PAQPDDVANP SQSNQFNLYG NDMAHNDVEY DSTGQWRNMQ QVGEVGMTRD 850 860 870 880 890 900 HSNTAACLKW SDRKCLECND SAYMFEDQCY DVCPVHTYPL DKFQAEEDEQ DDEVTRGPVN 910 920 930 940 950 960 PYSSSPMDHS LLMSNSLDDK QDPLQAEDRR RRSSLTQLVE VPSRVCAACD RSCLECYGAL 970 980 990 1000 1010 1020 ASQCSTCSPG SQLRKILNET FCYAYVVRST GMASVVDISK MDDRDTQQYM TGTTVLLLVS 1030 1040 1050 1060 1070 1080 VIFTLMGVAV AGGIVYHRRA MARSNELYSR VSLVPGDESD SDEDELFTAH FPARKSGVNI 1090 1100 YRDEAPSEKI FEEDEISHLV P
	Isoform 1 (F). See P26016.	FASTA	–	–
	Isoform 1-X (E). See P26016.	FASTA	–	–
	Isoform 2 (C) (D) (F). See P26016.	FASTA	–	–

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A unique Kex2-like endoprotease from Drosophila melanogaster is expressed in the central nervous system during early embryogenesis." Hayflick J.S., Wolfgang W.J., Forte M.A., Thomas G. J. Neurosci. 12:705-717(1992) [PubMed: 1545235] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Oregon-R. Tissue: Head.
[2]	"Generation of structural and functional diversity in furin-like proteins in Drosophila melanogaster by alternative splicing of the DFur1 gene." Roebroek A.J.M., Creemers J.W.M., Pauli I.G.L., Bogaert T., Van de Ven W.J.M. EMBO J. 12:1853-1870(1993) [PubMed: 8491178] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Strain: Oregon-R and Tuebingen. Tissue: Embryo.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M81431 mRNA. Translation: AAA28467.1. L12370, L12369 Genomic DNA. Translation: AAA28545.1. L12372 mRNA. Translation: AAA28548.1. AE014297 Genomic DNA. Translation: AAN14051.1.
RefSeq	NP_733102.1.
UniGene	Dm.1740
3D structure databases
HSSP	HSSP built from PDB template 1P8J based on UniProtKB P23188.
ModBase	Search...
Protein family/group databases
MEROPS	S08.048.
Genome annotation databases
Ensembl	FBgn0004509. Drosophila melanogaster. [Contig view]
GeneID	47220.
Organism-specific databases
FlyBase	FBgn0004509. Fur1.
Phylogenomic databases
OMA	P30430. NEGRYMA.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-013401-MON.
BRENDA	3.4.21.75. 48.
Gene expression databases
ArrayExpress	P30430.
GermOnline	CG10772. Drosophila melanogaster.
Family and domain databases
InterPro	IPR006212. Furin_repeat. IPR000209. Pept_S8_S53. IPR015500. Peptidase_S8_subtilisin-rel. IPR002884. PrprotnconvertsP. [Graphical view]
Gene3D	G3DSA:3.40.50.200. Pept_S8_S53. 1 hit.
PANTHER	PTHR10795. SubtilSerProt. 1 hit.
Pfam	PF01483. P_proprotein. 1 hit. PF00082. Peptidase_S8. 1 hit. [Graphical view]
PRINTS	PR00723. SUBTILISIN.
ProDom	PD000717. PrprotnconvertsP. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00261. FU. 2 hits. [Graphical view]
PROSITE	PS00136. SUBTILASE_ASP. 1 hit. PS00137. SUBTILASE_HIS. 1 hit. PS00138. SUBTILASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	838963.

Entry information

Entry name

FUR1C_DROME

Accession

Primary (citable) accession number: P30430
Secondary accession number(s): Q27235, Q9VBR5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	June 1, 2001
Last modified:	June 16, 2009
This is version 97 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project