Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P30411 (BKRB2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 151. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
B2 bradykinin receptor

Short name=B2R
Short name=BK-2 receptor
Gene names
Name:BDKRB2
Synonyms:BKR2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length391 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor for bradykinin. It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system.

Subunit structure

Forms a complex with PECAM1 and GNAQ. Interacts with PECAM1. Ref.11

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Ubiquitous. Widespread in normal smooth muscle tissue and neurons. Ref.5

Sequence similarities

Belongs to the G-protein coupled receptor 1 family. Bradykinin receptor subfamily. BDKRB2 sub-subfamily.

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainTransmembrane
Transmembrane helix
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
Lipoprotein
Palmitate
Phosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processG-protein coupled receptor signaling pathway

Traceable author statement Ref.1. Source: ProtInc

arachidonic acid secretion

Inferred from direct assay PubMed 17077303. Source: BHF-UCL

blood circulation

Non-traceable author statement Ref.5. Source: ProtInc

cell surface receptor signaling pathway

Traceable author statement Ref.2. Source: ProtInc

inflammatory response

Inferred by curator PubMed 17077303. Source: BHF-UCL

metabolic process

Traceable author statement PubMed 8302267. Source: GOC

negative regulation of intrinsic apoptotic signaling pathway in response to osmotic stress by p53 class mediator

Inferred from electronic annotation. Source: Ensembl

negative regulation of peptidyl-serine phosphorylation

Inferred from electronic annotation. Source: Ensembl

positive regulation of cytosolic calcium ion concentration

Traceable author statement PubMed 10653985. Source: ProtInc

regulation of vascular permeability

Inferred by curator PubMed 17077303. Source: BHF-UCL

regulation of vasoconstriction

Inferred by curator PubMed 17077303. Source: BHF-UCL

response to salt stress

Inferred from electronic annotation. Source: Ensembl

smooth muscle contraction

Inferred by curator PubMed 17077303. Source: BHF-UCL

transmembrane receptor protein tyrosine kinase signaling pathway

Traceable author statement PubMed 10653985. Source: ProtInc

vasoconstriction

Inferred from electronic annotation. Source: InterPro

vasodilation

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentendosome

Inferred from direct assay PubMed 17077303. Source: BHF-UCL

integral component of plasma membrane

Traceable author statement Ref.2. Source: ProtInc

plasma membrane

Inferred from direct assay PubMed 17077303. Source: BHF-UCL

   Molecular_functionbradykinin receptor activity

Inferred from direct assay PubMed 17077303. Source: BHF-UCL

phosphatidylinositol phospholipase C activity

Traceable author statement PubMed 8302267. Source: ProtInc

protease binding

Inferred from physical interaction PubMed 17077303. Source: BHF-UCL

protein binding

Inferred from physical interaction Ref.11. Source: UniProtKB

protein heterodimerization activity

Inferred from physical interaction PubMed 10993080. Source: BHF-UCL

type 1 angiotensin receptor binding

Inferred from physical interaction PubMed 10993080. Source: BHF-UCL

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

KNG1P010422EBI-6623386,EBI-6623273

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Long (identifier: P30411-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Short (identifier: P30411-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-27: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 391391B2 bradykinin receptor
PRO_0000069190

Regions

Topological domain1 – 6060Extracellular Potential
Transmembrane61 – 8424Helical; Name=1; Potential
Topological domain85 – 939Cytoplasmic Potential
Transmembrane94 – 11825Helical; Name=2; Potential
Topological domain119 – 13113Extracellular Potential
Transmembrane132 – 15322Helical; Name=3; Potential
Topological domain154 – 17522Cytoplasmic Potential
Transmembrane176 – 19823Helical; Name=4; Potential
Topological domain199 – 22123Extracellular Potential
Transmembrane222 – 24827Helical; Name=5; Potential
Topological domain249 – 26719Cytoplasmic Potential
Transmembrane268 – 29225Helical; Name=6; Potential
Topological domain293 – 31119Extracellular Potential
Transmembrane312 – 33524Helical; Name=7; Potential
Topological domain336 – 39156Cytoplasmic Potential

Amino acid modifications

Modified residue1561Phosphotyrosine By similarity
Modified residue3471Phosphotyrosine By similarity
Modified residue3661Phosphoserine By similarity
Modified residue3691Phosphothreonine By similarity
Modified residue3731Phosphoserine; by GRK6 Ref.12
Modified residue3751Phosphoserine; by GRK6 Ref.12
Lipidation3511S-palmitoyl cysteine Potential
Glycosylation301N-linked (GlcNAc...) Potential
Glycosylation391N-linked (GlcNAc...) Potential
Glycosylation2071N-linked (GlcNAc...) Potential
Disulfide bond130 ↔ 211 By similarity

Natural variations

Alternative sequence1 – 2727Missing in isoform Short.
VSP_001865
Natural variant141R → C. Ref.6 Ref.8
Corresponds to variant rs1046248 [ dbSNP | Ensembl ].
VAR_003457
Natural variant3541G → E. Ref.8
Corresponds to variant rs2227279 [ dbSNP | Ensembl ].
VAR_012284

Sequences

Sequence LengthMass (Da)Tools
Isoform Long [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: 066940ED3D18BB66

FASTA39144,461
        10         20         30         40         50         60 
MFSPWKISMF LSVREDSVPT TASFSADMLN VTLQGPTLNG TFAQSKCPQV EWLGWLNTIQ 

        70         80         90        100        110        120 
PPFLWVLFVL ATLENIFVLS VFCLHKSSCT VAEIYLGNLA AADLILACGL PFWAITISNN 

       130        140        150        160        170        180 
FDWLFGETLC RVVNAIISMN LYSSICFLML VSIDRYLALV KTMSMGRMRG VRWAKLYSLV 

       190        200        210        220        230        240 
IWGCTLLLSS PMLVFRTMKE YSDEGHNVTA CVISYPSLIW EVFTNMLLNV VGFLLPLSVI 

       250        260        270        280        290        300 
TFCTMQIMQV LRNNEMQKFK EIQTERRATV LVLVVLLLFI ICWLPFQIST FLDTLHRLGI 

       310        320        330        340        350        360 
LSSCQDERII DVITQIASFM AYSNSCLNPL VYVIVGKRFR KKSWEVYQGV CQKGGCRSEP 

       370        380        390 
IQMENSMGTL RTSISVERQI HKLQDWAGSR Q 

« Hide

Isoform Short [UniParc].

Checksum: 8FDC67EFC6600FB6
Show »

FASTA36441,441

References

« Hide 'large scale' references
[1]"Cloning and pharmacological characterization of a human bradykinin (BK-2) receptor."
Hess J.F.R., Borkowski J.A., Young G.S., Strader C.D., Ransom R.W.
Biochem. Biophys. Res. Commun. 184:260-268(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
[2]"Human bradykinin B2 receptor: nucleotide sequence analysis and assignment to chromosome 14."
Powell S.J., Slynn G., Thomas C., Hopkins B., Briggs I., Graham A.
Genomics 15:435-438(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM SHORT).
[3]"Cloned murine bradykinin receptor exhibits a mixed B1 and B2 pharmacological selectivity."
McIntyre P., Phillips E., Skidmore E., Brown M., Webb M.
Mol. Pharmacol. 44:346-355(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
[4]"Molecular cloning, functional expression and pharmacological characterization of a human bradykinin B2 receptor gene."
Eggerickx D., Raspe E., Bertrand D., Vassart G., Parmentier M.
Biochem. Biophys. Res. Commun. 187:1306-1313(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM SHORT).
[5]"Structure and chromosomal localization of the gene (BDKRB2) encoding human bradykinin B2 receptor."
Ma J.-X., Wang D.-Z., Ward D.C., Chen L., Dessai T., Chao J., Chao L.
Genomics 23:362-369(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM LONG), TISSUE SPECIFICITY.
[6]"Identification of polymorphic sites of the human bradykinin B2 receptor gene."
Braun A., Kammerer S., Boehme E., Mueller B., Roscher A.A.
Biochem. Biophys. Res. Commun. 211:234-240(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG), VARIANT CYS-14.
[7]"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
Warren C.N., Aronstam R.S., Sharma S.V.
Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
Tissue: Lung.
[8]SeattleSNPs variation discovery resource
Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS CYS-14 AND GLU-354.
[9]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
Tissue: Lung.
[10]"Structure of the bradykinin B2 receptors' amino terminus."
Abdalla S., Godovac-Zimmermann J., Braun A., Roscher A.A., Mueller-Esterl W., Quitterer U.
Biochemistry 35:7514-7519(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-9 AND 17-30.
Tissue: Foreskin.
[11]"Regulation of G protein-coupled receptor activities by the platelet-endothelial cell adhesion molecule, PECAM-1."
Yeh J.C., Otte L.A., Frangos J.A.
Biochemistry 47:9029-9039(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PECAM1.
[12]"Determination of bradykinin B2 receptor in vivo phosphorylation sites and their role in receptor function."
Blaukat A., Pizard A., Breit A., Wernstedt C., Alhenc-Gelas F., Muller-Esterl W., Dikic I.
J. Biol. Chem. 276:40431-40440(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT SER-373 AND SER-375.
+Additional computationally mapped references.

Web resources

Wikipedia

Bradykinin receptor entry

SeattleSNPs

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M88714 mRNA. Translation: AAB02793.1.
S56772 Genomic DNA. Translation: AAB25765.1.
X69680 mRNA. Translation: CAA49360.1.
S45489 Genomic DNA. Translation: AAB23467.1.
L27594 Genomic DNA. No translation available.
X86165 mRNA. Translation: CAA60109.1.
X86164 mRNA. Translation: CAA60108.1.
AY275465 mRNA. Translation: AAP32297.1.
AF378542 Genomic DNA. Translation: AAK56376.1.
BC074894 mRNA. Translation: AAH74894.1.
BC074895 mRNA. Translation: AAH74895.1.
CCDSCCDS9942.1. [P30411-1]
PIRJQ1488. JH0712.
RefSeqNP_000614.1. NM_000623.3. [P30411-1]
UniGeneHs.654542.
Hs.726620.

3D structure databases

ProteinModelPortalP30411.
SMRP30411. Positions 61-341.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid107093. 4 interactions.
IntActP30411. 1 interaction.
MINTMINT-107081.
STRING9606.ENSP00000307713.

Chemistry

BindingDBP30411.
ChEMBLCHEMBL3157.
GuidetoPHARMACOLOGY42.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP30411.

Polymorphism databases

DMDM2506481.

Proteomic databases

MaxQBP30411.
PaxDbP30411.
PRIDEP30411.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000306005; ENSP00000307713; ENSG00000168398. [P30411-1]
ENST00000539359; ENSP00000438376; ENSG00000168398. [P30411-2]
ENST00000542454; ENSP00000439459; ENSG00000168398. [P30411-2]
ENST00000554311; ENSP00000450482; ENSG00000168398. [P30411-1]
GeneID624.
KEGGhsa:624.
UCSCuc001yfg.2. human. [P30411-1]

Organism-specific databases

CTD624.
GeneCardsGC14P096671.
HGNCHGNC:1030. BDKRB2.
HPAHPA050841.
MIM113503. gene.
neXtProtNX_P30411.
PharmGKBPA80.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG148810.
HOGENOMHOG000010314.
HOVERGENHBG103183.
InParanoidP30411.
KOK03916.
OMAHKSSCTV.
OrthoDBEOG7HHWSV.
PhylomeDBP30411.
TreeFamTF330024.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.

Gene expression databases

BgeeP30411.
CleanExHS_BDKRB2.
GenevestigatorP30411.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR001504. Brdyknn_2_rcpt.
IPR000496. Brdyknn_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00425. BRADYKININR.
PR00994. BRADYKINNB2R.
PR00237. GPCRRHODOPSN.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSBDKRB2. human.
GeneWikiBradykinin_receptor_B2.
GenomeRNAi624.
NextBio2522.
PROP30411.
SOURCESearch...

Entry information

Entry nameBKRB2_HUMAN
AccessionPrimary (citable) accession number: P30411
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: November 1, 1997
Last modified: July 9, 2014
This is version 151 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 14

Human chromosome 14: entries, gene names and cross-references to MIM

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries