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P30411

- BKRB2_HUMAN

UniProt

P30411 - BKRB2_HUMAN

Protein

B2 bradykinin receptor

Gene

BDKRB2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 153 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Receptor for bradykinin. It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system.

    GO - Molecular functioni

    1. bradykinin receptor activity Source: BHF-UCL
    2. phosphatidylinositol phospholipase C activity Source: ProtInc
    3. protease binding Source: BHF-UCL
    4. protein binding Source: UniProtKB
    5. protein heterodimerization activity Source: BHF-UCL
    6. type 1 angiotensin receptor binding Source: BHF-UCL

    GO - Biological processi

    1. arachidonic acid secretion Source: BHF-UCL
    2. blood circulation Source: ProtInc
    3. cell surface receptor signaling pathway Source: ProtInc
    4. G-protein coupled receptor signaling pathway Source: ProtInc
    5. inflammatory response Source: BHF-UCL
    6. metabolic process Source: GOC
    7. negative regulation of intrinsic apoptotic signaling pathway in response to osmotic stress by p53 class mediator Source: Ensembl
    8. negative regulation of peptidyl-serine phosphorylation Source: Ensembl
    9. positive regulation of cytosolic calcium ion concentration Source: ProtInc
    10. regulation of vascular permeability Source: BHF-UCL
    11. regulation of vasoconstriction Source: BHF-UCL
    12. response to salt stress Source: Ensembl
    13. smooth muscle contraction Source: BHF-UCL
    14. transmembrane receptor protein tyrosine kinase signaling pathway Source: ProtInc
    15. vasoconstriction Source: InterPro
    16. vasodilation Source: Ensembl

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Enzyme and pathway databases

    ReactomeiREACT_14819. Peptide ligand-binding receptors.
    REACT_18283. G alpha (q) signalling events.
    REACT_19231. G alpha (i) signalling events.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    B2 bradykinin receptor
    Short name:
    B2R
    Short name:
    BK-2 receptor
    Gene namesi
    Name:BDKRB2
    Synonyms:BKR2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 14

    Organism-specific databases

    HGNCiHGNC:1030. BDKRB2.

    Subcellular locationi

    GO - Cellular componenti

    1. endosome Source: BHF-UCL
    2. integral component of plasma membrane Source: ProtInc
    3. plasma membrane Source: BHF-UCL

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA80.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 391391B2 bradykinin receptorPRO_0000069190Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi30 – 301N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi39 – 391N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi130 ↔ 211PROSITE-ProRule annotation
    Modified residuei156 – 1561PhosphotyrosineBy similarity
    Glycosylationi207 – 2071N-linked (GlcNAc...)Sequence Analysis
    Modified residuei347 – 3471PhosphotyrosineBy similarity
    Lipidationi351 – 3511S-palmitoyl cysteineSequence Analysis
    Modified residuei366 – 3661PhosphoserineBy similarity
    Modified residuei369 – 3691PhosphothreonineBy similarity
    Modified residuei373 – 3731Phosphoserine; by GRK61 Publication
    Modified residuei375 – 3751Phosphoserine; by GRK61 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

    Proteomic databases

    MaxQBiP30411.
    PaxDbiP30411.
    PRIDEiP30411.

    PTM databases

    PhosphoSiteiP30411.

    Expressioni

    Tissue specificityi

    Ubiquitous. Widespread in normal smooth muscle tissue and neurons.1 Publication

    Gene expression databases

    BgeeiP30411.
    CleanExiHS_BDKRB2.
    GenevestigatoriP30411.

    Organism-specific databases

    HPAiHPA050841.

    Interactioni

    Subunit structurei

    Forms a complex with PECAM1 and GNAQ. Interacts with PECAM1.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    KNG1P010422EBI-6623386,EBI-6623273

    Protein-protein interaction databases

    BioGridi107093. 4 interactions.
    IntActiP30411. 1 interaction.
    MINTiMINT-107081.
    STRINGi9606.ENSP00000307713.

    Structurei

    3D structure databases

    ProteinModelPortaliP30411.
    SMRiP30411. Positions 61-341.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 6060ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini85 – 939CytoplasmicSequence Analysis
    Topological domaini119 – 13113ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini154 – 17522CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini199 – 22123ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini249 – 26719CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini293 – 31119ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini336 – 39156CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei61 – 8424Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei94 – 11825Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei132 – 15322Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei176 – 19823Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei222 – 24827Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei268 – 29225Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei312 – 33524Helical; Name=7Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family. Bradykinin receptor subfamily. BDKRB2 sub-subfamily.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG148810.
    HOGENOMiHOG000010314.
    HOVERGENiHBG103183.
    InParanoidiP30411.
    KOiK03916.
    OMAiHKSSCTV.
    OrthoDBiEOG7HHWSV.
    PhylomeDBiP30411.
    TreeFamiTF330024.

    Family and domain databases

    Gene3Di1.20.1070.10. 1 hit.
    InterProiIPR001504. Brdyknn_2_rcpt.
    IPR000496. Brdyknn_rcpt.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    [Graphical view]
    PfamiPF00001. 7tm_1. 1 hit.
    [Graphical view]
    PRINTSiPR00425. BRADYKININR.
    PR00994. BRADYKINNB2R.
    PR00237. GPCRRHODOPSN.
    PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform Long (identifier: P30411-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MFSPWKISMF LSVREDSVPT TASFSADMLN VTLQGPTLNG TFAQSKCPQV    50
    EWLGWLNTIQ PPFLWVLFVL ATLENIFVLS VFCLHKSSCT VAEIYLGNLA 100
    AADLILACGL PFWAITISNN FDWLFGETLC RVVNAIISMN LYSSICFLML 150
    VSIDRYLALV KTMSMGRMRG VRWAKLYSLV IWGCTLLLSS PMLVFRTMKE 200
    YSDEGHNVTA CVISYPSLIW EVFTNMLLNV VGFLLPLSVI TFCTMQIMQV 250
    LRNNEMQKFK EIQTERRATV LVLVVLLLFI ICWLPFQIST FLDTLHRLGI 300
    LSSCQDERII DVITQIASFM AYSNSCLNPL VYVIVGKRFR KKSWEVYQGV 350
    CQKGGCRSEP IQMENSMGTL RTSISVERQI HKLQDWAGSR Q 391
    Length:391
    Mass (Da):44,461
    Last modified:November 1, 1997 - v2
    Checksum:i066940ED3D18BB66
    GO
    Isoform Short (identifier: P30411-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-27: Missing.

    Show »
    Length:364
    Mass (Da):41,441
    Checksum:i8FDC67EFC6600FB6
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti14 – 141R → C.2 Publications
    Corresponds to variant rs1046248 [ dbSNP | Ensembl ].
    VAR_003457
    Natural varianti354 – 3541G → E.1 Publication
    Corresponds to variant rs2227279 [ dbSNP | Ensembl ].
    VAR_012284

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 2727Missing in isoform Short. 2 PublicationsVSP_001865Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M88714 mRNA. Translation: AAB02793.1.
    S56772 Genomic DNA. Translation: AAB25765.1.
    X69680 mRNA. Translation: CAA49360.1.
    S45489 Genomic DNA. Translation: AAB23467.1.
    L27594 Genomic DNA. No translation available.
    X86165 mRNA. Translation: CAA60109.1.
    X86164 mRNA. Translation: CAA60108.1.
    AY275465 mRNA. Translation: AAP32297.1.
    AF378542 Genomic DNA. Translation: AAK56376.1.
    BC074894 mRNA. Translation: AAH74894.1.
    BC074895 mRNA. Translation: AAH74895.1.
    CCDSiCCDS9942.1. [P30411-1]
    PIRiJH0712. JQ1488.
    RefSeqiNP_000614.1. NM_000623.3. [P30411-1]
    UniGeneiHs.654542.
    Hs.726620.

    Genome annotation databases

    EnsembliENST00000539359; ENSP00000438376; ENSG00000168398. [P30411-2]
    ENST00000542454; ENSP00000439459; ENSG00000168398. [P30411-2]
    ENST00000554311; ENSP00000450482; ENSG00000168398. [P30411-1]
    GeneIDi624.
    KEGGihsa:624.
    UCSCiuc001yfg.2. human. [P30411-1]

    Polymorphism databases

    DMDMi2506481.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Web resourcesi

    Wikipedia

    Bradykinin receptor entry

    SeattleSNPs

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M88714 mRNA. Translation: AAB02793.1 .
    S56772 Genomic DNA. Translation: AAB25765.1 .
    X69680 mRNA. Translation: CAA49360.1 .
    S45489 Genomic DNA. Translation: AAB23467.1 .
    L27594 Genomic DNA. No translation available.
    X86165 mRNA. Translation: CAA60109.1 .
    X86164 mRNA. Translation: CAA60108.1 .
    AY275465 mRNA. Translation: AAP32297.1 .
    AF378542 Genomic DNA. Translation: AAK56376.1 .
    BC074894 mRNA. Translation: AAH74894.1 .
    BC074895 mRNA. Translation: AAH74895.1 .
    CCDSi CCDS9942.1. [P30411-1 ]
    PIRi JH0712. JQ1488.
    RefSeqi NP_000614.1. NM_000623.3. [P30411-1 ]
    UniGenei Hs.654542.
    Hs.726620.

    3D structure databases

    ProteinModelPortali P30411.
    SMRi P30411. Positions 61-341.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 107093. 4 interactions.
    IntActi P30411. 1 interaction.
    MINTi MINT-107081.
    STRINGi 9606.ENSP00000307713.

    Chemistry

    BindingDBi P30411.
    ChEMBLi CHEMBL3157.
    GuidetoPHARMACOLOGYi 42.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei P30411.

    Polymorphism databases

    DMDMi 2506481.

    Proteomic databases

    MaxQBi P30411.
    PaxDbi P30411.
    PRIDEi P30411.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000539359 ; ENSP00000438376 ; ENSG00000168398 . [P30411-2 ]
    ENST00000542454 ; ENSP00000439459 ; ENSG00000168398 . [P30411-2 ]
    ENST00000554311 ; ENSP00000450482 ; ENSG00000168398 . [P30411-1 ]
    GeneIDi 624.
    KEGGi hsa:624.
    UCSCi uc001yfg.2. human. [P30411-1 ]

    Organism-specific databases

    CTDi 624.
    GeneCardsi GC14P096671.
    HGNCi HGNC:1030. BDKRB2.
    HPAi HPA050841.
    MIMi 113503. gene.
    neXtProti NX_P30411.
    PharmGKBi PA80.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG148810.
    HOGENOMi HOG000010314.
    HOVERGENi HBG103183.
    InParanoidi P30411.
    KOi K03916.
    OMAi HKSSCTV.
    OrthoDBi EOG7HHWSV.
    PhylomeDBi P30411.
    TreeFami TF330024.

    Enzyme and pathway databases

    Reactomei REACT_14819. Peptide ligand-binding receptors.
    REACT_18283. G alpha (q) signalling events.
    REACT_19231. G alpha (i) signalling events.

    Miscellaneous databases

    ChiTaRSi BDKRB2. human.
    GeneWikii Bradykinin_receptor_B2.
    GenomeRNAii 624.
    NextBioi 2522.
    PROi P30411.
    SOURCEi Search...

    Gene expression databases

    Bgeei P30411.
    CleanExi HS_BDKRB2.
    Genevestigatori P30411.

    Family and domain databases

    Gene3Di 1.20.1070.10. 1 hit.
    InterProi IPR001504. Brdyknn_2_rcpt.
    IPR000496. Brdyknn_rcpt.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    [Graphical view ]
    Pfami PF00001. 7tm_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00425. BRADYKININR.
    PR00994. BRADYKINNB2R.
    PR00237. GPCRRHODOPSN.
    PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and pharmacological characterization of a human bradykinin (BK-2) receptor."
      Hess J.F.R., Borkowski J.A., Young G.S., Strader C.D., Ransom R.W.
      Biochem. Biophys. Res. Commun. 184:260-268(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
    2. "Human bradykinin B2 receptor: nucleotide sequence analysis and assignment to chromosome 14."
      Powell S.J., Slynn G., Thomas C., Hopkins B., Briggs I., Graham A.
      Genomics 15:435-438(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM SHORT).
    3. "Cloned murine bradykinin receptor exhibits a mixed B1 and B2 pharmacological selectivity."
      McIntyre P., Phillips E., Skidmore E., Brown M., Webb M.
      Mol. Pharmacol. 44:346-355(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
    4. "Molecular cloning, functional expression and pharmacological characterization of a human bradykinin B2 receptor gene."
      Eggerickx D., Raspe E., Bertrand D., Vassart G., Parmentier M.
      Biochem. Biophys. Res. Commun. 187:1306-1313(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM SHORT).
    5. "Structure and chromosomal localization of the gene (BDKRB2) encoding human bradykinin B2 receptor."
      Ma J.-X., Wang D.-Z., Ward D.C., Chen L., Dessai T., Chao J., Chao L.
      Genomics 23:362-369(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM LONG), TISSUE SPECIFICITY.
    6. "Identification of polymorphic sites of the human bradykinin B2 receptor gene."
      Braun A., Kammerer S., Boehme E., Mueller B., Roscher A.A.
      Biochem. Biophys. Res. Commun. 211:234-240(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG), VARIANT CYS-14.
    7. "cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
      Warren C.N., Aronstam R.S., Sharma S.V.
      Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
      Tissue: Lung.
    8. SeattleSNPs variation discovery resource
      Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS CYS-14 AND GLU-354.
    9. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG).
      Tissue: Lung.
    10. Cited for: PROTEIN SEQUENCE OF 1-9 AND 17-30.
      Tissue: Foreskin.
    11. "Regulation of G protein-coupled receptor activities by the platelet-endothelial cell adhesion molecule, PECAM-1."
      Yeh J.C., Otte L.A., Frangos J.A.
      Biochemistry 47:9029-9039(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PECAM1.
    12. "Determination of bradykinin B2 receptor in vivo phosphorylation sites and their role in receptor function."
      Blaukat A., Pizard A., Breit A., Wernstedt C., Alhenc-Gelas F., Muller-Esterl W., Dikic I.
      J. Biol. Chem. 276:40431-40440(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT SER-373 AND SER-375.

    Entry informationi

    Entry nameiBKRB2_HUMAN
    AccessioniPrimary (citable) accession number: P30411
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 1, 1993
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 153 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. Human chromosome 14
      Human chromosome 14: entries, gene names and cross-references to MIM
    3. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    4. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    5. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3