P30411 (BKRB2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
B2 bradykinin receptor
Short name=B2R
Short name=BK-2 receptor

Gene names

Name:	BDKRB2
Synonyms:	BKR2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	391 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for bradykinin. It is associated with G proteins that activate a phosphatidylinositol-calcium second messenger system.
Subunit structure	Forms a complex with PECAM1 and GNAQ. Interacts with PECAM1. Ref.11
Subcellular location	Cell membrane; Multi-pass membrane protein.
Tissue specificity	Ubiquitous. Widespread in normal smooth muscle tissue and neurons. Ref.5
Sequence similarities	Belongs to the G-protein coupled receptor 1 family. Bradykinin receptor subfamily. BDKRB2 sub-subfamily.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	arachidonic acid secretion Inferred from direct assay PubMed 17077303. Source: BHF-UCL elevation of cytosolic calcium ion concentration Traceable author statement PubMed 10653985. Source: ProtInc inflammatory response Inferred by curator PubMed 17077303. Source: BHF-UCL negative regulation of apoptotic process Inferred from electronic annotation. Source: Compara negative regulation of peptidyl-serine phosphorylation Inferred from electronic annotation. Source: Compara regulation of vascular permeability Inferred by curator PubMed 17077303. Source: BHF-UCL regulation of vasoconstriction Inferred by curator PubMed 17077303. Source: BHF-UCL response to salt stress Inferred from electronic annotation. Source: Compara smooth muscle contraction Inferred by curator PubMed 17077303. Source: BHF-UCL transmembrane receptor protein tyrosine kinase signaling pathway Traceable author statement PubMed 10653985. Source: ProtInc
Cellular_component	endosome Inferred from direct assay PubMed 17077303. Source: BHF-UCL integral to plasma membrane Traceable author statement Ref.2. Source: ProtInc
Molecular_function	bradykinin receptor activity Inferred from direct assay PubMed 17077303. Source: BHF-UCL phosphatidylinositol phospholipase C activity Traceable author statement PubMed 8302267. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Long (identifier: P30411-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: P30411-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-27: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 391	391	B2 bradykinin receptor		PRO_0000069190
Regions
	Topological domain	1 – 60	60	Extracellular Potential
	Transmembrane	61 – 84	24	Helical; Name=1; Potential
	Topological domain	85 – 93	9	Cytoplasmic Potential
	Transmembrane	94 – 118	25	Helical; Name=2; Potential
	Topological domain	119 – 131	13	Extracellular Potential
	Transmembrane	132 – 153	22	Helical; Name=3; Potential
	Topological domain	154 – 175	22	Cytoplasmic Potential
	Transmembrane	176 – 198	23	Helical; Name=4; Potential
	Topological domain	199 – 221	23	Extracellular Potential
	Transmembrane	222 – 248	27	Helical; Name=5; Potential
	Topological domain	249 – 267	19	Cytoplasmic Potential
	Transmembrane	268 – 292	25	Helical; Name=6; Potential
	Topological domain	293 – 311	19	Extracellular Potential
	Transmembrane	312 – 335	24	Helical; Name=7; Potential
	Topological domain	336 – 391	56	Cytoplasmic Potential
Amino acid modifications
	Modified residue	156	1	Phosphotyrosine By similarity
	Modified residue	347	1	Phosphotyrosine By similarity
	Modified residue	366	1	Phosphoserine By similarity
	Modified residue	369	1	Phosphothreonine By similarity
	Modified residue	373	1	Phosphoserine; by GRK6 Ref.12
	Modified residue	375	1	Phosphoserine; by GRK6 Ref.12
	Lipidation	351	1	S-palmitoyl cysteine Potential
	Glycosylation	30	1	N-linked (GlcNAc...) Potential
	Glycosylation	39	1	N-linked (GlcNAc...) Potential
	Glycosylation	207	1	N-linked (GlcNAc...) Potential
	Disulfide bond	130 ↔ 211		By similarity
Natural variations
	Alternative sequence	1 – 27	27	Missing in isoform Short.		VSP_001865
	Natural variant	14	1	R → C. Ref.6 Ref.8 Corresponds to variant rs1046248 [ dbSNP | Ensembl ].		VAR_003457
	Natural variant	354	1	G → E. Ref.8 Corresponds to variant rs2227279 [ dbSNP | Ensembl ].		VAR_012284

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 066940ED3D18BB66	FASTA	391	44,461
	10 20 30 40 50 60 MFSPWKISMF LSVREDSVPT TASFSADMLN VTLQGPTLNG TFAQSKCPQV EWLGWLNTIQ 70 80 90 100 110 120 PPFLWVLFVL ATLENIFVLS VFCLHKSSCT VAEIYLGNLA AADLILACGL PFWAITISNN 130 140 150 160 170 180 FDWLFGETLC RVVNAIISMN LYSSICFLML VSIDRYLALV KTMSMGRMRG VRWAKLYSLV 190 200 210 220 230 240 IWGCTLLLSS PMLVFRTMKE YSDEGHNVTA CVISYPSLIW EVFTNMLLNV VGFLLPLSVI 250 260 270 280 290 300 TFCTMQIMQV LRNNEMQKFK EIQTERRATV LVLVVLLLFI ICWLPFQIST FLDTLHRLGI 310 320 330 340 350 360 LSSCQDERII DVITQIASFM AYSNSCLNPL VYVIVGKRFR KKSWEVYQGV CQKGGCRSEP 370 380 390 IQMENSMGTL RTSISVERQI HKLQDWAGSR Q
	Isoform Short [UniParc]. Checksum: 8FDC67EFC6600FB6	FASTA	364	41,441
	10 20 30 40 50 60 MLNVTLQGPT LNGTFAQSKC PQVEWLGWLN TIQPPFLWVL FVLATLENIF VLSVFCLHKS 70 80 90 100 110 120 SCTVAEIYLG NLAAADLILA CGLPFWAITI SNNFDWLFGE TLCRVVNAII SMNLYSSICF 130 140 150 160 170 180 LMLVSIDRYL ALVKTMSMGR MRGVRWAKLY SLVIWGCTLL LSSPMLVFRT MKEYSDEGHN 190 200 210 220 230 240 VTACVISYPS LIWEVFTNML LNVVGFLLPL SVITFCTMQI MQVLRNNEMQ KFKEIQTERR 250 260 270 280 290 300 ATVLVLVVLL LFIICWLPFQ ISTFLDTLHR LGILSSCQDE RIIDVITQIA SFMAYSNSCL 310 320 330 340 350 360 NPLVYVIVGK RFRKKSWEVY QGVCQKGGCR SEPIQMENSM GTLRTSISVE RQIHKLQDWA GSRQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and pharmacological characterization of a human bradykinin (BK-2) receptor." Hess J.F.R., Borkowski J.A., Young G.S., Strader C.D., Ransom R.W. Biochem. Biophys. Res. Commun. 184:260-268(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
[2]	"Human bradykinin B2 receptor: nucleotide sequence analysis and assignment to chromosome 14." Powell S.J., Slynn G., Thomas C., Hopkins B., Briggs I., Graham A. Genomics 15:435-438(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM SHORT).
[3]	"Cloned murine bradykinin receptor exhibits a mixed B1 and B2 pharmacological selectivity." McIntyre P., Phillips E., Skidmore E., Brown M., Webb M. Mol. Pharmacol. 44:346-355(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM SHORT).
[4]	"Molecular cloning, functional expression and pharmacological characterization of a human bradykinin B2 receptor gene." Eggerickx D., Raspe E., Bertrand D., Vassart G., Parmentier M. Biochem. Biophys. Res. Commun. 187:1306-1313(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM SHORT).
[5]	"Structure and chromosomal localization of the gene (BDKRB2) encoding human bradykinin B2 receptor." Ma J.-X., Wang D.-Z., Ward D.C., Chen L., Dessai T., Chao J., Chao L. Genomics 23:362-369(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM LONG), TISSUE SPECIFICITY.
[6]	"Identification of polymorphic sites of the human bradykinin B2 receptor gene." Braun A., Kammerer S., Boehme E., Mueller B., Roscher A.A. Biochem. Biophys. Res. Commun. 211:234-240(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM LONG), VARIANT CYS-14.
[7]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Warren C.N., Aronstam R.S., Sharma S.V. Submitted (APR-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG). Tissue: Lung.
[8]	SeattleSNPs variation discovery resource Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS CYS-14 AND GLU-354.
[9]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM LONG). Tissue: Lung.
[10]	"Structure of the bradykinin B2 receptors' amino terminus." Abdalla S., Godovac-Zimmermann J., Braun A., Roscher A.A., Mueller-Esterl W., Quitterer U. Biochemistry 35:7514-7519(1996) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-9 AND 17-30. Tissue: Foreskin.
[11]	"Regulation of G protein-coupled receptor activities by the platelet-endothelial cell adhesion molecule, PECAM-1." Yeh J.C., Otte L.A., Frangos J.A. Biochemistry 47:9029-9039(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH PECAM1.
[12]	"Determination of bradykinin B2 receptor in vivo phosphorylation sites and their role in receptor function." Blaukat A., Pizard A., Breit A., Wernstedt C., Alhenc-Gelas F., Muller-Esterl W., Dikic I. J. Biol. Chem. 276:40431-40440(2001) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION AT SER-373 AND SER-375.
+	Additional computationally mapped references.

Web resources

Wikipedia

Bradykinin receptor entry

SeattleSNPs

Cross-references

Sequence databases
EMBL GenBank DDBJ	M88714 mRNA. Translation: AAB02793.1. S56772 Genomic DNA. Translation: AAB25765.1. X69680 mRNA. Translation: CAA49360.1. S45489 Genomic DNA. Translation: AAB23467.1. L27594 Genomic DNA. No translation available. X86165 mRNA. Translation: CAA60109.1. X86164 mRNA. Translation: CAA60108.1. AY275465 mRNA. Translation: AAP32297.1. AF378542 Genomic DNA. Translation: AAK56376.1. BC074894 mRNA. Translation: AAH74894.1. BC074895 mRNA. Translation: AAH74895.1.
IPI	IPI00026534. IPI00218209.
PIR	JQ1488. JH0712.
RefSeq	NP_000614.1. NM_000623.3.
UniGene	Hs.654542. Hs.726620.
3D structure databases
ProteinModelPortal	P30411.
ModBase	Search...
Protein-protein interaction databases
STRING	9606.ENSP00000307713.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P30411.
Polymorphism databases
DMDM	2506481.
Proteomic databases
PaxDb	P30411.
PRIDE	P30411.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000306005; ENSP00000307713; ENSG00000168398. ENST00000539359; ENSP00000438376; ENSG00000168398. ENST00000542454; ENSP00000439459; ENSG00000168398. ENST00000554311; ENSP00000450482; ENSG00000168398.
GeneID	624.
KEGG	hsa:624.
UCSC	uc001yfg.2. human.
Organism-specific databases
CTD	624.
GeneCards	GC14P096671.
HGNC	HGNC:1030. BDKRB2.
HPA	HPA050841.
MIM	113503. gene.
neXtProt	NX_P30411.
PharmGKB	PA80.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG148810.
HOGENOM	HOG000010314.
HOVERGEN	HBG103183.
InParanoid	P30411.
KO	K03916.
OMA	GHNVTAC.
OrthoDB	EOG4DR9CD.
PhylomeDB	P30411.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
Bgee	P30411.
CleanEx	HS_BDKRB2.
Genevestigator	P30411.
GermOnline	ENSG00000168398. Homo sapiens.
Family and domain databases
InterPro	IPR001504. Brdyknn_2_rcpt. IPR000496. Brdyknn_rcpt. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00425. BRADYKININR. PR00994. BRADYKINNB2R. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P30411.
ChEMBL	CHEMBL3157.
ChiTaRS	BDKRB2. human.
GenomeRNAi	624.
NextBio	2522.
SOURCE	Search...

Entry information

Entry name

BKRB2_HUMAN

Accession

Primary (citable) accession number: P30411

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 138 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.