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Protein

Peptidyl-prolyl cis-trans isomerase F, mitochondrial

Gene

PPIF

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Involved in regulation of the mitochondrial permeability transition pore (mPTP). It is proposed that its association with the mPTP is masking a binding site for inhibiting inorganic phosphate (Pi) and promotes the open probability of the mPTP leading to apoptosis or necrosis; the requirement of the PPIase activity for this function is debated. In cooperation with mitochondrial TP53 is involved in activating oxidative stress-induced necrosis. Involved in modulation of mitochondrial membrane F1F0 ATP synthase activity and regulation of mitochondrial matrix adenine nucleotide levels. Has anti-apoptotic activity independently of mPTP and in cooperation with BCL2 inhibits cytochrome c-dependent apoptosis (By similarity).By similarity

Catalytic activityi

Peptidylproline (omega=180) = peptidylproline (omega=0).

Enzyme regulationi

Binds cyclosporin A (CsA). Is displaced by CsA from the mPTP leading to a lower open probability of the mPTP (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase, Rotamase
Biological processApoptosis, Necrosis

Names & Taxonomyi

Protein namesi
Recommended name:
Peptidyl-prolyl cis-trans isomerase F, mitochondrial (EC:5.2.1.8)
Short name:
PPIase F
Alternative name(s):
Cyclophilin D
Short name:
CyP-D
Short name:
CypD
Cyclophilin F
Rotamase F
Gene namesi
Name:PPIF
Synonyms:CYP3
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 28

Organism-specific databases

VGNCiVGNC:33198 PPIF

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 30MitochondrionAdd BLAST30
ChainiPRO_000006414931 – 208Peptidyl-prolyl cis-trans isomerase F, mitochondrialAdd BLAST178

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei68N6-acetyllysine; alternateBy similarity1
Modified residuei68N6-succinyllysine; alternateBy similarity1
Modified residuei87N6-succinyllysineBy similarity1
Modified residuei168N6-acetyllysineBy similarity1
Modified residuei176N6-succinyllysineBy similarity1
Modified residuei191N6-succinyllysineBy similarity1
Modified residuei204S-nitrosocysteineBy similarity1

Post-translational modificationi

Deacteylated at Lys-168 by SIRT3.By similarity

Keywords - PTMi

Acetylation, S-nitrosylation

Proteomic databases

PaxDbiP30404
PeptideAtlasiP30404
PRIDEiP30404

Expressioni

Gene expression databases

BgeeiENSBTAG00000016711

Interactioni

Subunit structurei

Associates with the mitochondrial membrane ATP synthase F1F0 ATP synthase; the association is increased by inorganic phosphate (Pi) and decreased by cyclosporin A (CsA) (By similarity). Interacts with ATP5F1B; ATP5H and ATP5O (PubMed:19801635). Interacts with SLC25A3; the interaction is impaired by CsA. Interacts with BCL2; the interaction is impaired by CsA. Interacts with TP53; the association implicates preferentially tetrameric TP53, is induced by oxidative stress and is impaired by CsA. Interacts with C1QBP. Interacts with MCUR1. Component of the mitochondrial permeability transition pore complex (mPTPC), at least composed of SPG7, VDAC1 and PPIF. Interacts with SPG7 (By similarity).By similarity1 Publication

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000022213

Structurei

3D structure databases

SMRiP30404
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini50 – 206PPIase cyclophilin-typePROSITE-ProRule annotationAdd BLAST157

Sequence similaritiesi

Belongs to the cyclophilin-type PPIase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0865 Eukaryota
COG0652 LUCA
GeneTreeiENSGT00760000119119
HOGENOMiHOG000065981
HOVERGENiHBG001065
InParanoidiP30404
KOiK09565
OMAiELKHTGS
OrthoDBiEOG091G0BGL

Family and domain databases

Gene3Di2.40.100.10, 1 hit
InterProiView protein in InterPro
IPR029000 Cyclophilin-like_dom_sf
IPR024936 Cyclophilin-type_PPIase
IPR020892 Cyclophilin-type_PPIase_CS
IPR002130 Cyclophilin-type_PPIase_dom
PANTHERiPTHR11071 PTHR11071, 1 hit
PfamiView protein in Pfam
PF00160 Pro_isomerase, 1 hit
PIRSFiPIRSF001467 Peptidylpro_ismrse, 1 hit
PRINTSiPR00153 CSAPPISMRASE
SUPFAMiSSF50891 SSF50891, 1 hit
PROSITEiView protein in PROSITE
PS00170 CSA_PPIASE_1, 1 hit
PS50072 CSA_PPIASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P30404-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMLALRCGPR LLGLLSGPRS AHLRLPAVRA CSSGSGSHGS SSSSGNPLVY
60 70 80 90 100
LDVGADGQPL GRVVLELKAD VVPKTAENFR ALCTGEKGFG YKGSTFHRVI
110 120 130 140 150
PSFMCQAGDF TNHNGTGGKS IYGSRFPDEN FKLKHEGPGV LSMANAGPNT
160 170 180 190 200
NGSQFFICTI KTDWLDGKHV VFGHVKEGMD VVKKIESFGS KSGKTSKKIV

ITDCGQLS
Length:208
Mass (Da):22,019
Last modified:March 6, 2013 - v3
Checksum:i087239A93D68A512
GO

Sequence cautioni

The sequence AAT02663 differs from that shown. Reason: Frameshift at several positions.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti30A → V in AAT02663 (Ref. 1) Curated1
Sequence conflicti30A → V in EH176460 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY594335 mRNA Translation: AAT02663.1 Sequence problems.
EH176460 mRNA No translation available.
AAFC03123307 Genomic DNA No translation available.
PIRiPC1237
RefSeqiNP_001001597.1, NM_001001597.1
UniGeneiBt.9791

Genome annotation databases

EnsembliENSBTAT00000022213; ENSBTAP00000022213; ENSBTAG00000016711
GeneIDi414346
KEGGibta:414346

Similar proteinsi

Entry informationi

Entry nameiPPIF_BOVIN
AccessioniPrimary (citable) accession number: P30404
Secondary accession number(s): Q6PMK8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: March 6, 2013
Last modified: March 28, 2018
This is version 123 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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