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Protein

Orotate phosphoribosyltransferase 2

Gene

URA10

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).By similarity

Catalytic activityi

Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate.

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes UMP from orotate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Orotate phosphoribosyltransferase 1 (URA5), Orotate phosphoribosyltransferase 2 (URA10)
  2. Orotidine 5'-phosphate decarboxylase (URA3)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UMP from orotate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei109 – 10915-phosphoribose 1-diphosphate; shared with dimeric partnerBy similarity
Binding sitei110 – 11015-phosphoribose 1-diphosphateBy similarity
Binding sitei113 – 11315-phosphoribose 1-diphosphate; shared with dimeric partnerBy similarity
Binding sitei115 – 11515-phosphoribose 1-diphosphate; shared with dimeric partnerBy similarity
Binding sitei139 – 1391OrotateBy similarity
Binding sitei167 – 1671OrotateBy similarity

GO - Molecular functioni

  • orotate phosphoribosyltransferase activity Source: SGD

GO - Biological processi

  • 'de novo' pyrimidine nucleobase biosynthetic process Source: SGD
  • 'de novo' UMP biosynthetic process Source: UniProtKB-UniPathway
  • pyrimidine ribonucleoside biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Pyrimidine biosynthesis

Enzyme and pathway databases

BioCyciYEAST:YMR271C-MONOMER.
UniPathwayiUPA00070; UER00119.

Names & Taxonomyi

Protein namesi
Recommended name:
Orotate phosphoribosyltransferase 2 (EC:2.4.2.10)
Short name:
OPRT 2
Short name:
OPRTase 2
Gene namesi
Name:URA10
Ordered Locus Names:YMR271C
ORF Names:YM8156.13C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR271C.
SGDiS000004884. URA10.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 227227Orotate phosphoribosyltransferase 2PRO_0000110805Add
BLAST

Proteomic databases

MaxQBiP30402.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

BioGridi35449. 15 interactions.
DIPiDIP-2790N.
IntActiP30402. 1 interaction.
MINTiMINT-492469.

Structurei

3D structure databases

ProteinModelPortaliP30402.
SMRiP30402. Positions 8-226.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni41 – 422Orotate bindingBy similarity
Regioni79 – 8025-phosphoribose 1-diphosphate bindingBy similarity
Regioni135 – 14395-phosphoribose 1-diphosphate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00550000076056.
HOGENOMiHOG000037974.
InParanoidiP30402.
KOiK00762.
OMAiSAYAHTI.
OrthoDBiEOG76X69W.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01208. PyrE.
InterProiIPR023031. OPRT.
IPR004467. Or_phspho_trans_dom.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR00336. pyrE. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P30402-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSASTTSLEE YQKTFLELGL ECKALRFGSF KLNSGRQSPY FFNLSLFNSG
60 70 80 90 100
KLLANLATAY ATAIIQSELK FDVIFGPAYK GIPLAAIVCV KLAEIGGTKF
110 120 130 140 150
QGIQYAFNRK KVKDHGEGGI IVGASLEDKR VLIIDDVMTA GTAINEAFEI
160 170 180 190 200
ISIAQGRVVG CIVALDRQEV IHESDPERTS ATQSVSKRYN VPVLSIVSLT
210 220
QVVQFMGNRL SPEQKSAIEN YRKAYGI
Length:227
Mass (Da):24,811
Last modified:November 1, 1995 - v3
Checksum:iADA6C21380935EDD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti143 – 1431A → R in CAA36440 (PubMed:2182197).Curated
Sequence conflicti158 – 1581V → L in CAA36440 (PubMed:2182197).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52194 Genomic DNA. Translation: CAA36440.1.
X66375 Genomic DNA. Translation: CAA47016.1.
Z49260 Genomic DNA. Translation: CAA89254.1.
AY557976 Genomic DNA. Translation: AAS56302.1.
BK006946 Genomic DNA. Translation: DAA10171.1.
PIRiS48223. XJBY10.
RefSeqiNP_013998.1. NM_001182778.1.

Genome annotation databases

EnsemblFungiiYMR271C; YMR271C; YMR271C.
GeneIDi855313.
KEGGisce:YMR271C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52194 Genomic DNA. Translation: CAA36440.1.
X66375 Genomic DNA. Translation: CAA47016.1.
Z49260 Genomic DNA. Translation: CAA89254.1.
AY557976 Genomic DNA. Translation: AAS56302.1.
BK006946 Genomic DNA. Translation: DAA10171.1.
PIRiS48223. XJBY10.
RefSeqiNP_013998.1. NM_001182778.1.

3D structure databases

ProteinModelPortaliP30402.
SMRiP30402. Positions 8-226.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35449. 15 interactions.
DIPiDIP-2790N.
IntActiP30402. 1 interaction.
MINTiMINT-492469.

Proteomic databases

MaxQBiP30402.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR271C; YMR271C; YMR271C.
GeneIDi855313.
KEGGisce:YMR271C.

Organism-specific databases

EuPathDBiFungiDB:YMR271C.
SGDiS000004884. URA10.

Phylogenomic databases

GeneTreeiENSGT00550000076056.
HOGENOMiHOG000037974.
InParanoidiP30402.
KOiK00762.
OMAiSAYAHTI.
OrthoDBiEOG76X69W.

Enzyme and pathway databases

UniPathwayiUPA00070; UER00119.
BioCyciYEAST:YMR271C-MONOMER.

Miscellaneous databases

PROiP30402.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
HAMAPiMF_01208. PyrE.
InterProiIPR023031. OPRT.
IPR004467. Or_phspho_trans_dom.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR00336. pyrE. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequencing of URA10, a second gene encoding orotate phosphoribosyl transferase in Saccharomyces cerevisiae."
    de Montigny J., Kern L., Hubert J.-C., Lacroute F.
    Curr. Genet. 17:105-111(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The byp1-3 allele of the Saccharomyces cerevisiae GGS1/TPS1 gene and its multi-copy suppressor tRNA(GLN) (CAG): Ggs1/Tps1 protein levels restraining growth on fermentable sugars and trehalose accumulation."
    Hohmann S., Van Dijck P., Luyten K., Thevelein J.M.
    Curr. Genet. 26:295-301(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPYRX_YEAST
AccessioniPrimary (citable) accession number: P30402
Secondary accession number(s): D6W097
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: November 1, 1995
Last modified: June 8, 2016
This is version 131 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

There are two genes coding for OPRT in yeast.
Present with 815 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.