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P30372 (ACM2_CHICK) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Muscarinic acetylcholine receptor M2
Gene names
Name:CHRM2
Synonyms:CM2
OrganismGallus gallus (Chicken) [Reference proteome]
Taxonomic identifier9031 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length466 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is adenylate cyclase inhibition.

Subcellular location

Cell membrane; Multi-pass membrane protein. Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein.

Miscellaneous

This receptor has a high affinity for pirenzepine.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM2 sub-subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 466466Muscarinic acetylcholine receptor M2
PRO_0000069026

Regions

Topological domain1 – 2525Extracellular By similarity
Transmembrane26 – 4823Helical; Name=1; By similarity
Topological domain49 – 6214Cytoplasmic By similarity
Transmembrane63 – 8321Helical; Name=2; By similarity
Topological domain84 – 10017Extracellular By similarity
Transmembrane101 – 12222Helical; Name=3; By similarity
Topological domain123 – 14220Cytoplasmic By similarity
Transmembrane143 – 16523Helical; Name=4; By similarity
Topological domain166 – 18722Extracellular By similarity
Transmembrane188 – 21023Helical; Name=5; By similarity
Topological domain211 – 388178Cytoplasmic By similarity
Transmembrane389 – 40921Helical; Name=6; By similarity
Topological domain410 – 42314Extracellular By similarity
Transmembrane424 – 44320Helical; Name=7; By similarity
Topological domain444 – 46623Cytoplasmic By similarity

Amino acid modifications

Modified residue4461Phosphothreonine Potential
Modified residue4501Phosphothreonine Potential
Modified residue4651Phosphothreonine Potential
Glycosylation21N-linked (GlcNAc...) Potential
Glycosylation31N-linked (GlcNAc...) Potential
Glycosylation81N-linked (GlcNAc...) Potential
Disulfide bond99 ↔ 179 By similarity

Sequences

Sequence LengthMass (Da)Tools
P30372 [UniParc].

Last modified April 1, 1993. Version 1.
Checksum: 2E3D8241D6168995

FASTA46651,565
        10         20         30         40         50         60 
MNNSTYINSS SENVIALESP YKTIEVVFIV LVAGSLSLVT IIGNILVMVS IKVNRHLQTV 

        70         80         90        100        110        120 
NNYFLFSLAC ADLIIGIFSM NLYTLYTVIG YWPLGPVVCD LWLALDYVVS NASVMNLLII 

       130        140        150        160        170        180 
SFDRYFCVTK PLTYPVKRTT KMAGMMIAAA WVLSFILWAP AILFWQFIVG GRTVPDKDCY 

       190        200        210        220        230        240 
IQFFSNPAVT FGTAIAAFYL PVIIMTVLYW QISRASKSRI KKGKKEAAQN QDPVSPSLVQ 

       250        260        270        280        290        300 
GKIVKPNNNN IPTSSDGLEH NKVQNGKTTG ESVMENCVQG EEKDSSNDST SVSVVPSNTK 

       310        320        330        340        350        360 
EDEAAKDASQ ISASQDHLKV ENSKLTCIRI VTKSQKGDCC APTNTTVEIV GTNGDEKQNS 

       370        380        390        400        410        420 
VARKIVKMTK QPAKKKPPPS REKKVTRTIL AILLAFIITW TPYNVMVLIN SFCASCIPGT 

       430        440        450        460 
VWTIGYWLCY INSTINPACY ALCNATFKKT FKHLLMCHYK NIGATR

« Hide

References

[1]"Embryonic chick heart expresses multiple muscarinic acetylcholine receptor subtypes. Isolation and characterization of a gene encoding a novel m2 muscarinic acetylcholine receptor with high affinity for pirenzepine."
Tietje K.M., Nathanson N.M.
J. Biol. Chem. 266:17382-17387(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M73217 Genomic DNA. Translation: AAB04106.1.
IPIIPI00576864.
PIRA40972.
RefSeqNP_001025936.1. NM_001030765.1.
UniGeneGga.10240.
Gga.49677.

3D structure databases

ProteinModelPortalP30372.
ModBaseSearch...

Protein-protein interaction databases

STRING9031.ENSGALP00000020999.

Protein family/group databases

GPCRDBSearch...

Proteomic databases

PaxDbP30372.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSGALT00000021029; ENSGALP00000020999; ENSGALG00000012894.
GeneID418126.
KEGGgga:418126.

Organism-specific databases

CTD1129.

Phylogenomic databases

eggNOGNOG250863.
GeneTreeENSGT00690000101881.
HOGENOMHOG000231484.
HOVERGENHBG105720.
InParanoidP30372.
KOK04130.
OrthoDBEOG41JZCC.

Family and domain databases

InterProIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001065. Musac_M2_rcpt.
IPR000995. Musac_rcpt.
[Graphical view]
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR00237. GPCRRHODOPSN.
PR00243. MUSCARINICR.
PR00539. MUSCRINICM2R.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20821333.

Entry information

Entry nameACM2_CHICK
AccessionPrimary (citable) accession number: P30372
Entry history
Integrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: April 3, 2013
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries

SIMILARITY comments

Index of protein domains and families

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