P30366 (PP11_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein phosphatase PP1 isozyme 1
EC=3.1.3.16

Gene names

Name:	TOPP1
Synonyms:	PP1
Ordered Locus Names:	At2g29400
ORF Names:	F16P2.22

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	318 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 manganese ion per subunit By similarity.
Sequence similarities	Belongs to the PPP phosphatase family. PP-1 subfamily.

Ontologies

Keywords
Ligand	Iron Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein dephosphorylation Inferred from genetic interaction Ref.1. Source: TAIR
Cellular_component	protein phosphatase type 1 complex Inferred from sequence or structural similarity Ref.2. Source: TAIR
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine phosphatase activity Inferred from direct assay PubMed 21222654. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 318	318	Serine/threonine-protein phosphatase PP1 isozyme 1		PRO_0000058797
Sites
	Active site	138	1	Proton donor By similarity
	Metal binding	77	1	Iron By similarity
	Metal binding	79	1	Iron By similarity
	Metal binding	105	1	Iron By similarity
	Metal binding	105	1	Manganese By similarity
	Metal binding	137	1	Manganese By similarity
	Metal binding	186	1	Manganese By similarity
	Metal binding	260	1	Manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	P30366 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: DAAA11674B148294	FASTA	318	36,279
	10 20 30 40 50 60 MAEKPAQEQE QKRAMEPAVL DDIIRRLVEF RNTRPGSGKQ VHLSEGEIRQ LCAVSKEIFL 70 80 90 100 110 120 QQPNLLELEA PIKICGDIHG QYSDLLRLFE YGGFPPEANY LFLGDYVDRG KQSLETICLL 130 140 150 160 170 180 LAYKIKYPEN FFLLRGNHES ASINRIYGFY DECKRRFNVR LWKIFTDCFN CLPVAALIDD 190 200 210 220 230 240 RILCMHGGIS PELKSLDQIR NIARPMDIPE SGLVCDLLWS DPSGDVGWGM NDRGVSYTFG 250 260 270 280 290 300 ADKVAEFLEK HDMDLICRAH QVVEDGYEFF AERQLVTVFS APNYCGEFDN AGAMMSIDES 310 LMCSFQILKP SEKKSPFL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complementation of the cs dis2-11 cell cycle mutant of Schizosaccharomyces pombe by a protein phosphatase from Arabidopsis thaliana." Nitschke K., Fleig U., Schell J., Palme K. EMBO J. 11:1327-1333(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Expression of multiple type 1 phosphoprotein phosphatases in Arabidopsis thaliana." Smith R.D., Walker J.C. Plant Mol. Biol. 21:307-316(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Arabidopsis PPP family of serine/threonine phosphatases." Farkas I., Dombradi V., Miskei M., Szabados L., Koncz C. Trends Plant Sci. 12:169-176(2007) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X64328 mRNA. Translation: CAA45611.1. M93408 mRNA. Translation: AAA32723.1. AC004561 Genomic DNA. Translation: AAC95198.1. CP002685 Genomic DNA. Translation: AEC08250.1. AY080871 mRNA. Translation: AAL87342.1. AY142593 mRNA. Translation: AAN13162.1.
IPI	IPI00529116.
PIR	S20882.
RefSeq	NP_180501.1. NM_128494.4.
UniGene	At.282.
3D structure databases
ProteinModelPortal	P30366.
SMR	P30366. Positions 20-311.
ModBase	Search...
Protein-protein interaction databases
IntAct	P30366. 1 interaction.
STRING	3702.AT2G29400.1-P.
Proteomic databases
PaxDb	P30366.
PRIDE	P30366.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G29400.1; AT2G29400.1; AT2G29400.
GeneID	817489.
KEGG	ath:AT2G29400.
Organism-specific databases
TAIR	At2g29400.
Phylogenomic databases
eggNOG	COG0639.
HOGENOM	HOG000172697.
InParanoid	P30366.
KO	K06269.
OMA	VPLMESE.
PhylomeDB	P30366.
ProtClustDB	CLSN2913212.
Gene expression databases
Genevestigator	P30366.
GermOnline	AT2G29400. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR004843. Metallo_PEstase_dom. IPR006186. Ser/Thr-sp_prot-phosphatase. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PRINTS	PR00114. STPHPHTASE.
SMART	SM00156. PP2Ac. 1 hit. [Graphical view]
PROSITE	PS00125. SER_THR_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PP11_ARATH

Accession

Primary (citable) accession number: P30366

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	May 1, 2013
This is version 110 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program