Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Serine/threonine-protein phosphatase PP1 isozyme 1

Gene

TOPP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine-protein phosphatase that possesses phosphatase activity toward para-nitrophenyl phosphate (pNPP) in vitro (PubMed:21222654, PubMed:26943172). Acts as negative regulator of abscisic acid (ABA) signaling. In vitro, can dephosphorylate and inhibit the kinase activity of SRK2E/SNRK2.6, an activator of ABA signaling.2 Publications

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.1 Publication

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Enzyme regulationi

Phosphatase activity is strongly reduced by the protein phosphatase inhibitor 2 (I-2) (PubMed:21222654, PubMed:26943172). Phosphatase activity is strongly reduced by PYL11, an abscisic acid (ABA) receptor (PubMed:26943172).2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi77Manganese 1By similarity1
Metal bindingi79Manganese 1By similarity1
Metal bindingi105Manganese 1By similarity1
Metal bindingi105Manganese 2By similarity1
Metal bindingi137Manganese 2By similarity1
Active sitei138Proton donorBy similarity1
Metal bindingi186Manganese 2By similarity1
Metal bindingi260Manganese 2By similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • protein serine/threonine phosphatase activity Source: TAIR

Keywordsi

Molecular functionHydrolase, Protein phosphatase
LigandManganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PP1 isozyme 1Curated (EC:3.1.3.161 Publication)
Alternative name(s):
Type one protein phosphatase 11 Publication
Gene namesi
Name:TOPP11 Publication
Synonyms:PP1Imported
Ordered Locus Names:At2g29400Imported
ORF Names:F16P2.22Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G29400.
TAIRilocus:2043122. AT2G29400.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions, but mutant seedlings exhibit hypersensitivity to abscisic acid (ABA) or salt treatments.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000587971 – 318Serine/threonine-protein phosphatase PP1 isozyme 1Add BLAST318

Proteomic databases

PaxDbiP30366.
PRIDEiP30366.

Expressioni

Tissue specificityi

Expressed in cotyledons, leaves and flowers.1 Publication

Gene expression databases

ExpressionAtlasiP30366. baseline and differential.
GenevisibleiP30366. AT.

Interactioni

Subunit structurei

Interacts with I-2, SRK2D/SNRK2.2, SRK2I/SNRK2.3, SRK2A/SNRK2.4, SRK2E/SNRK2.6, SRK2C/SNRK2.8, PYL4, PYL9 and PYL11.1 Publication

Protein-protein interaction databases

BioGridi2839. 1 interactor.
IntActiP30366. 1 interactor.
STRINGi3702.AT2G29400.1.

Structurei

3D structure databases

ProteinModelPortaliP30366.
SMRiP30366.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-1 subfamily.Curated

Phylogenomic databases

eggNOGiENOG410IN84. Eukaryota.
ENOG410XXN0. LUCA.
HOGENOMiHOG000172697.
InParanoidiP30366.
KOiK06269.
OMAiHSVENII.
OrthoDBiEOG09360F05.
PhylomeDBiP30366.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiView protein in InterPro
IPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
IPR031675. STPPase_N.
PfamiView protein in Pfam
PF00149. Metallophos. 1 hit.
PF16891. STPPase_N. 1 hit.
PRINTSiPR00114. STPHPHTASE.
SMARTiView protein in SMART
SM00156. PP2Ac. 1 hit.
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiView protein in PROSITE
PS00125. SER_THR_PHOSPHATASE. 1 hit.

Sequencei

Sequence statusi: Complete.

P30366-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEKPAQEQE QKRAMEPAVL DDIIRRLVEF RNTRPGSGKQ VHLSEGEIRQ
60 70 80 90 100
LCAVSKEIFL QQPNLLELEA PIKICGDIHG QYSDLLRLFE YGGFPPEANY
110 120 130 140 150
LFLGDYVDRG KQSLETICLL LAYKIKYPEN FFLLRGNHES ASINRIYGFY
160 170 180 190 200
DECKRRFNVR LWKIFTDCFN CLPVAALIDD RILCMHGGIS PELKSLDQIR
210 220 230 240 250
NIARPMDIPE SGLVCDLLWS DPSGDVGWGM NDRGVSYTFG ADKVAEFLEK
260 270 280 290 300
HDMDLICRAH QVVEDGYEFF AERQLVTVFS APNYCGEFDN AGAMMSIDES
310
LMCSFQILKP SEKKSPFL
Length:318
Mass (Da):36,279
Last modified:April 1, 1993 - v1
Checksum:iDAAA11674B148294
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64328 mRNA. Translation: CAA45611.1.
M93408 mRNA. Translation: AAA32723.1.
AC004561 Genomic DNA. Translation: AAC95198.1.
CP002685 Genomic DNA. Translation: AEC08250.1.
AY080871 mRNA. Translation: AAL87342.1.
AY142593 mRNA. Translation: AAN13162.1.
PIRiS20882.
RefSeqiNP_180501.1. NM_128494.5.
UniGeneiAt.282.

Genome annotation databases

EnsemblPlantsiAT2G29400.1; AT2G29400.1; AT2G29400.
GeneIDi817489.
GrameneiAT2G29400.1; AT2G29400.1; AT2G29400.
KEGGiath:AT2G29400.

Similar proteinsi

Entry informationi

Entry nameiPP11_ARATH
AccessioniPrimary (citable) accession number: P30366
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 1, 1993
Last sequence update: April 1, 1993
Last modified: October 25, 2017
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families