P30363 (ASPG_BACLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
L-asparaginase
Short name=L-ASNase
EC=3.5.1.1

Alternative name(s):
L-asparagine amidohydrolase

Gene names

Name:

ansA

Organism

Bacillus licheniformis

Taxonomic identifier

1402 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	322 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-asparagine + H2O = L-aspartate + NH3.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the asparaginase 1 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	cellular amino acid metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	asparaginase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 322	322	L-asparaginase		PRO_0000171076
Regions
	Region	89 – 90	2	Substrate binding By similarity
Sites
	Active site	13	1	O-isoaspartyl threonine intermediate By similarity
	Binding site	56	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P30363 [UniParc]. Last modified April 1, 1993. Version 1. Checksum: 7048BEC52D0B1DFB	FASTA	322	35,442
	10 20 30 40 50 60 MNKKVALITT GGTIASRKTE SGRLAAGAIS GPELAEMCSL PEDVQIDVYP AFQLPSMHIT 70 80 90 100 110 120 FQHLLELKQT IERVFQDGGY DGAVVTHGTD TLEETAYFLD LTIEDERPVV VTGSQRAPEQ 130 140 150 160 170 180 QGTDAYTNIR HAVYTACSPD IKGAGTVVVF NERIFNARYV KKVHASNLQG FDVFGFGYLG 190 200 210 220 230 240 IIDNDKVYVY QKLLKRDVHQ LQRPLPAVDI VKCYLDGDGK FIRAAVREGV EGIVLEGVGR 250 260 270 280 290 300 GQVPPNMMAD IEQALNQGVY IVITTSAEEG EVYTTYDYAG SSYDLAKKGV ILGKDYDSKK 310 320 ARMKLAVLLA SYKEGIKDKF CY

References

[1]	"Lack of specific hybridization between the lep genes of Salmonella typhimurium and Bacillus licheniformis." van Dijl J.M., de Jong A., Smith H., Bron S., Venema G. FEMS Microbiol. Lett. 65:345-351(1991) [PubMed: 1916233] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z11497 Genomic DNA. Translation: CAA77574.1.
PIR	S18999.
3D structure databases
ProteinModelPortal	P30363.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR006034. Asparaginase/glutaminase. IPR020827. Asparaginase/glutaminase_CS. [Graphical view]
PANTHER	PTHR11707. Asp/Glutamnse. 1 hit.
Pfam	PF00710. Asparaginase. 1 hit. [Graphical view]
PIRSF	PIRSF001220. L-ASNase_gatD. 1 hit.
PRINTS	PR00139. ASNGLNASE.
SMART	SM00870. Asparaginase. 1 hit. [Graphical view]
SUPFAM	SSF53774. Asp/Glutamnse. 1 hit.
PROSITE	PS00144. ASN_GLN_ASE_1. 1 hit. PS00917. ASN_GLN_ASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASPG_BACLI

Accession

Primary (citable) accession number: P30363

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 1, 1993
Last sequence update:	April 1, 1993
Last modified:	September 21, 2011
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program